|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Rapid reconstitution of NK1 cells after allogeneic transplantation is associated with a reduced incidence of graft-versus-host disease显示文摘The balance between immunostimulation and immunoregulation in T cell immunity is achieved by maintaining specific ratios of Th1, Th2, Th3 and Tr1 cells. Here, we investigate levels of type 1(IFN-gamma; NK1), type 2(IL-13; NK2), type 3(TGF-beta;NK3) and regulatory(IL-10; NKr) cytokines in peripheral blood to assess the cytokine profiles of natural killer(NK) cells following human allogeneic hematopoietic stem cell transplantation(allo-HSCT). NK2 and NK3 cell expansion was observed after allo-HSCT; levels of NKr cells reached donor levels at day 15, though levels of NK1 cells were consistently lower than donor levels until day 60 after allo-HSCT. Multivariate analysis showed that a higher level of NK1 cells by day 15 was associated with a lower overall risk of acute graft-versus-host disease(GVHD)(HR 0.157, P=0.010) as well as II-IV acute GVHD(HR0.260, P=0.059). Furthermore, higher levels of NK1 cells by day 15 were correlated with lower rates of cytomegalovirus(CMV)reactivation(HR 0.040, 0.005–0.348, P=0.003). These results indicate that rapid reconstitution of NK cells, especially NK1 cells,can help prevent the development of GVHD as well as CMV reactivation after allogeneic transplantation. | Xingxing Yu Lingling Xu Yingjun Chang Xiaojun Huang Xiangyu Zhao | 2018 | Science China(Life Sciences)2018，61，8： | 9 |
| 2 | Everyone has a donor:contribution of the Chinese experience to global practice of haploidentical hematopoietic stem cell transplantation显示文摘Human leukocyte antigen (HLA)-matched donors for hematopoietic stem cell transplantation (HSCT) have long been scarce in China. Haploidentical (haplo) donors are available for the vast majority of patients, but toxicity has limited this approach. Three new approaches for haplo-HSCT originated from Italy, China, and USA in 1990 and have been developed to world-renowned system up to now. The Chinese approach have been greatly improved by implementing new individualized conditioning regimens, donor selection based on non-HLA systems, risk-directed strategies for graft-versus-host disease and relapse, and infection management. Haplo-HSCT has exhibited similar efficacy to HLA-matched HSCT and has gradually become the predominant donor source and the first alternative donor choice for allo-HSCT in China. Registry-based analyses and multicenter studies adhering to international standards facilitated the transformation of the unique Chinese experience into an inspiration for the refinement of global practice.This review will focus on how the new era in which 'everyone has a donor' will become a reality in China. | Meng Lv Yingjun Chang Xiaojun Huang | 2019 | Frontiers of Medicine2019，13，1： | 8 |
| 3 | Platelet transfusion refractoriness after T-cell-replete haploidentical transplantation is associated with inferior clinical outcomes显示文摘Haploidentical stem cell transplantation (haplo-SCT) has been an alternative source of bone marrow for patients without human leukocyte antigen (HLA)-matched donors. The aim of this study was to investigate the relationships between platelet transfusion refractoriness (PTR) and clinical outcomes in the setting of haplo-SCT. Between May 2012 and March 2014, 345 patients who underwent unmanipulated haplo-SCT were retrospectively enrolled. PTR occurred in 20.6% of all patients. Patients in the PTR group experienced higher transplant-related mortality (TRM, 43.7% vs. 13.5%, P<0.001), lower overall survival (OS, 47.9%vs. 76.3%, P<0.001) and lower leukemia-free survival (LFS, 47.9% vs. 72.3%, P<0.001) compared to patients in the non-PTR group. The multivariate analysis showed that PTR was associated with TRM (P=0.002), LFS (P<0.001), and OS (P<0.001).The cumulative incidences of PTR in patients receiving >12 platelet (PLT) transfusions (third quartile of PLT transfusions) were higher than in patients receiving either >6 (second quartile) or >3 (first quartile) PLT transfusions (56.1% vs. 41.6% vs. 28.2%,respectively; P<0.001). The multivariate analysis also showed that PTR was associated with the number of PLT transfusions(P<0.001). PTR could predict poor transplant outcomes in patients who underwent haploidentical SCT. | Qiang Fu Lanping Xu Xiaohui Zhang Yu Wang Yingjun Chang Kaiyan Liu Xiaojun Huang | 2018 | Science China(Life Sciences)2018，61，5： | 7 |
| 4 | Improved clinical outcomes of rhG-CSF-mobilized blood and marrow haploidentical transplantation compared to propensity score-matched rhG-CSF-primed peripheral blood stem cell haploidentical transplantation:a multicenter study显示文摘The effects of haploidentical rhG-CSF-mobilized blood and marrow transplantation(HBMT) on hematological malignances are well established. Previous prospective single-center studies have demonstrated better survival after HBMT versus haploidentical rhG-CSF-mobilized peripheral blood stem cell transplantation(HPBSCT) for acute leukemia(AL) not in remission(NR) or in more than the second complete remission(>CR2). To test the hypothesis that HBMT is still superior to HPBSCT for patients with AL, multiple myeloma(MM), or non-Hodgkin lymphoma(NHL) in CR1/CR2 and for patients with chronic myeloid leukemia in the first and second chronic phase lacking a matched donor, we designed a propensity score method-based multicenter study.Hematopoietic recovery, acute graft-versus-host disease(aGVHD), and chronic GVHD were comparable between the HBMT group(n=168) and the HPBSCT group(n=42). No significant differences were found in non-relapse mortality rate(20.17%±3.58%and 27.24%±7.16%, P=0.18) or relapse rate(19.96%±3.72% and 28.49%±8.25%, P=0.32) between the HBMT group and the HPBSCT group. HBMT recipients had better overall survival(65.0%±4.2% and 54.2%±8.3%, P=0.037) and disease-free survival(59.9%±4.6% and 44.3%±8.7%, P=0.051). Multivariate analysis showed that HPBSCT was associated with poorer DFS(HR(95%CI), 1.639(0.995–2.699), P=0.052). Our comparisons showed that HBMT was superior to HPBSCT as a post-remission treatment for patients lacking an identical donor. | Xiangyu Zhao Feng Gao Xiaohui Zhang Yu Wang Lanping Xu Kaiyan Liu Xiaosu Zhao Yingjun Chang Han Wei Huan Chen Yuhong Chen Zhengfan Jiang Xiaojun Huang | 2016 | Science China(Life Sciences)2016，59，11： | 7 |
| 5 | Insight into vitronectin structural evolution on material surface chemistries: The mediation for cell adhesion显示文摘Biomaterial surface chemistry engenders profound consequences on cell adhesion and the ultimate tissue response by adsorbing proteins from extracellular matrix,where vitronectin(Vn)is involved as one of the crucial mediator proteins.Deciphering the adsorption behaviors of Vn in molecular scale provides a useful account of how to design biomaterial surfaces.But the details of structural dynamics and consequential biological effect remain elusive.Herein,both experimental and computational approaches were applied to delineate the conformational and orientational evolution of Vn during adsorption onto self-assembled monolayers(SAMs)terminating with-COOH,-NH2,-CH3 and-OH.To unravel the interplay between cell binding and the charge and wettability of material surface,somatomedin-B(SMB)domain of Vn holding the RGD cell-binding motif was employed in molecular dynamics(MD)simulations,with orientation initialized by Monte Carlo(MC)method.Experimental evidences including protein adsorption,cell adhesion and integrin gene expressions were thoroughly investigated.The adsorption of Vn on different surface chemistries showed very complex profiles.Cell adhesion was enabled on all Vn-adsorbed surfaces but with distinct mechanisms mostly determined by conformational change induced reorientation.Higher amount of Vn was observed on negatively charged surface(COOH)and hydrophobic surface(CH3).However,advantageous orientations defined by RGD loop conditions were only obtained on the charged surfaces(COOH and NH2).Specifically,COOH surface straightened up the Vn molecules and accumulated them into a higher density,whereas CH3 surface squashed Vn and stacked them into higher density multilayer by tracking adsorption but with the RGD loops restrained.These findings may have a broad implication on the understanding of Vn functionality and would help develop new strategies for designing advanced biomaterials. | Tianjie Li Lijing Hao Jiangyu Li Chang Du Yingjun Wang | 2020 | Bioactive Materials2020，5，4： | 4 |
| 6 | A wave of Foxp3^(+) regulatory T cell accumulation in the neonatal liver plays unique roles in maintaining self-tolerance显示文摘Newborn animals require tightly regulated local and systemic immune environments to govern the development and maturation of multiple organs/tissues even though the immune system itself is far from mature during the neonatal period.Regulatory T cells(Tregs)are essential for maintaining immune tolerance/homeostasis and modulating inflammatory responses.The features of Tregs in the neonatal liver under steady-state conditions are not well understood.The present study aimed to investigate the phenotype,functions,and significance of neonatal Tregs in the liver.We found a wave of thymus-derived Treg influx into the liver during 1–2 weeks of age.Depletion of these Tregs between days 7 and 11 after birth rapidly resulted in Th1-type liver inflammation and metabolic disorder.More Tregs in the neonatal liver than in the spleen underwent MHC II-dependent activation and proliferation,and the liver Tregs acquired stronger suppressive functions.The transcriptomic profile of these neonatal liver Tregs showed elevated expression of PPARγand T-bet and features of Tregs that utilize lipid metabolic machinery and are capable of regulating Th1 responses.The accumulation of Tregs with unique features in the neonatal liver is critical to ensure self-tolerance and liver maturation. | Mingyang Li Weijia Zhao Yifan Wang Lixue Jin Gaowen Jin Xiuyuan Sun Wei Wang Ke Wang Xi Xu Jie Hao Rong Jin Wenxian Fu Ying Sun Yingjun Chang Xiaojun Huang Xuyu Zhou Hounan Wu Kunshan Zhang Qing Ge | 2020 | Cellular & Molecular Immunology2020，17，5： | 4 |
| 7 | Two competitive nucleation mechanisms of calcium carbonate biomineralization in response to surface functionality in low calcium ion concentration solution显示文摘Four self-assembled monolayer surfaces terminated with–COOH,–OH,–NH_(2)and–CH_(3)functional groups are used to direct the biomineralization processes of calcium carbonate(CaCO_(3))in low Ca^(2+)concentration,and the mechanism of nucleation and initial crystallization within 12 h was further explored.On-COOH surface,nucleation occurs mainly via ion aggregation mechanism while prenucleation ions clusters may be also involved.On-OH and-NH_(2)surfaces,however,nucleation forms via calcium carbonate clusters,which aggregate in solution and then are adsorbed onto surfaces following with nucleation of amorphous calcium carbonate(ACC).Furthermore,strongly negative-charged-COOH surface facilitates the direct formation of calcites,and the-OH and-NH_(2)surfaces determine the formation of vaterites with preferred crystalline orientations.Neither ACC nor crystalline CaCO_(3)is observed on-CH_(3)surface.Our findings present a valuable model to understand the CaCO_(3)biomineralization pathway in natural system where functional groups composition plays a determining role during calcium carbonate crystallization. | Hua Deng Shuo Wang Xiumei Wang Chang Du Xingcan Shen Yingjun Wang Fuzhai Cui | 2015 | Regenerative Biomaterials2015，2，3： | 3 |
| 8 | Mechanistic insights into the adsorption and bioactivity of fibronectin on surfaces with varying chemistries by a combination of experimental strategies and molecular simulations显示文摘Fibronectin(Fn)is significant to the performance of biomaterials,and the chemistry of biomaterial surface play important roles in Fn adsorption and subsequent cell behavior.However,the“molecular scale”mechanism is still unclear.Herein,we combined experimental strategies with molecular simulations to solve this problem.We prepared self-assembled monolayers with varying chemistries,i.e.,SAMs-CH3,SAMs-NH2,SAMs-COOH and SAMs-OH,and characterized Fn adsorption and cell behaviors on them.Next,Monte Carlo method and all-atom molecular dynamics simulations were employed to reveal the orientation/conformation of Fn on surfaces.We found that SAMs-CH3 strongly adsorbed Fn via hydrophobic interactions,but show poor bioactivity as the low exposure of RGD/PHSRN motifs and the deformation of Fn.SAMs-NH2 and SAMs-COOH could adsorb Fn efficiently via vdW interactions,electrostatic interactions,hydrogen bonds and salt bridges.Fn exhibited excellent bioactivity for cell adhesion,proliferation and osteogenic differentiation as high exposure of bioactive motifs on SAMs-NH2,or as the activation of other inferior cell-binding motifs on SAMs-COOH.SAMs-OH showed poor Fn adsorption as the water film.However,the adsorbed Fn displayed non-negligible bioactivity due to high exposure of PHSRN motif and large degree of protein flexibility.We believe that the revealed mechanism presents great potential to rationally design Fn-activating biomaterials. | Lijing Hao Tianjie Li Lin Wang Xuetao Shi Yan Fan Chang Du Yingjun Wang | 2021 | Bioactive Materials2021，6，10： | 3 |
| 9 | Monodomain Liquid Crystals of Two-Dimensional Sheets by Boundary-Free Sheargraphy显示文摘Eliminating topological defects to achieve monodomain liquid crystals is highly significant for the fundamental studies of soft matter and building long-range ordered materials.However,liquid crystals are metastable and sensitive to external stimuli,such as flow,confinement,and electromagnetic fields,which cause their intrinsic polycrystallinity and topological defects.Here,we achieve the monodomain liquid crystals of graphene oxide over 30 cm through boundary-free sheargraphy.The obtained monodomain liquid crystals exhibit large-area uniform alignment of sheets,which has the same optical polarized angle and intensity.The monodomain liquid crystals provide bidirectionally ordered skeletons,which can be applied as lightweight thermal management materials with bidirectionally high thermal and electrical conductivity.Furthermore,we extend the controllable topology of two-dimensional colloids by introducing singularities and disclinations in monodomain liquid crystals.Topological structures with defect strength from−2 to+2 were realized.This work provides a facile methodology to study the structural order of soft matter at a macroscopic level,facilitating the fabrication of metamaterials with tunable and highly anisotropic architectures. | Min Cao Senping Liu Qingli Zhu Ya Wang Jingyu Ma Zeshen Li Dan Chang Enhui Zhu Xin Ming Yingjun Liu Yanqiu Jiang Zhen Xu Chao Gao | 2022 | Nano-Micro Letters2022，14，12： | 2 |
| 10 | Negative association of donor age with CD34+ cell dose in mixture allografts of G-CSF-primed bone marrow and G-CSF-mobilized peripheral blood harvests显示文摘 | Li Yan Chang Yingjun Xu Lanping Zhang Xiaohui Huang Xiaojun | 2014 | Chinese Medical Journal2014，，20： | 2 |
| 11 | Dynamic dispersion stability of graphene oxide with metal ions显示文摘Graphene oxide(GO),an important chemical precursor of graphene,can stably disperse in aqueous surrounding and undergo aggregation as metal cations introduced.The usual instability of GO with ions is caused by the shielding effect of ions and crosslinking between GO and ions.However,the dynamic stability of GO under ions exchange still remains unclear.Here,we investigated the dynamic dispersion stability of GO with metal ions and observed a redispersion behavior in concentrated Fe3+solution,other than permanent aggregation.The exchange with Fe3+ions drives the reversion of zeta(ζ)potential and enables the redispersion to individual GO-Fe3+complex sheets,following a dynamic electric double layer(EDL)mechanism.It is found that the specifically strong electrostatic shielding effect and coordination attraction between Fe3+and functional oxygen groups allows the selective redispersion of GO in concentrated Fe3+solution.The revealed dynamic dispersion stability complements our understanding on the dispersive stability of GO and can be utilized to fabricate graphene-metal hybrids for rich applications. | Yinghui He Yingjun Liu Fan Guo Kai Pang Bo Fang Ya Wang Dan Chang Zhen Xu Chao Gao | 2020 | Chinese Chemical Letters2020，31，6： | 2 |
| 12 | Performance of a two-stage multi-effect desalination system based on humidification-dehumidification process 显示文摘 | Kang Huifang Yang Yingjun Chang Zehui | 2014 | Desalination2014，344，1： | 1 |
| 13 | Experimental investigation of a novel multi-effect solar desalination system based on humidification- dehumidification process 显示文摘 | Chang Zehui Zheng Hongfei Yang Yingjun | 2014 | Renewalbe Energy2014，，： | 1 |
| 14 | Precise Thermoplastic Processing of Graphene Oxide Layered Solid by Polymer Intercalation显示文摘The processing capability is vital for the wide applications of materials to forge structures as-demand.Graphene-based macroscopic materials have shown excellent mechanical and functional properties.However,different from usual polymers and metals,graphene solids exhibit limited deformability and processibility for precise forming.Here,we present a precise thermoplastic forming of graphene materials by polymer intercalation from graphene oxide(GO)precursor.The intercalated polymer enables the thermoplasticity of GO solids by thermally activated motion of polymer chains.We detect a critical minimum containing of intercalated polymer that can expand the interlayer spacing exceeding 1.4 nm to activate thermoplasticity,which becomes the criteria for thermal plastic forming of GO solids.By thermoplastic forming,the flat GO-composite films are forged to Gaussian curved shapes and imprinted to have surface relief patterns with size precision down to 360 nm.The plastic-formed structures maintain the structural integration with outstanding electrical(3.07×10^(5) S m^(−1))and thermal conductivity(745.65 W m^(−1) K^(−1))after removal of polymers.The thermoplastic strategy greatly extends the forming capability of GO materials and other layered materials and promises versatile structural designs for more broad applications. | Zeshen Li Fan Guo Kai Pang Jiahao Lin Qiang Gao Yance Chen Dan Chang Ya Wang Senping Liu Yi Han Yingjun Liu Zhen Xu Chao Gao | 2022 | Nano-Micro Letters2022，14，1： | 1 |
| 15 | Controlling the strontium-doping in calcium phosphate microcapsules through yeastregulated biomimetic mineralization显示文摘Yeast cells have controllable biosorption on metallic ions during metabolism.However,few studies were dedicated to using yeast-regulated biomimetic mineralization process to control the strontium-doped positions in calcium phosphate microcapsules.In this study,the yeast cells were allowed to pre-adsorb strontium ions metabolically and then served as sacrificing template for the precipitation and calcination of mineral shell.The pre-adsorption enabled the microorganism to enrich of strontium ions into the inner part of the microcapsules,which ensured a slow-release profile of the trace element from the microcapsule.The co-culture with human marrow stromal cells showed that gene expressions of alkaline phosphatase and Collagen-I were promoted.The promotion of osteogenic differentiation was further confirmed in the 3D culture of cell-material complexes.The strategy using living microorganism as‘smart doping apparatus’to control incorporation of trace element into calcium phosphate paved a pathway to new functional materials for hard tissue regeneration. | Miaojun Huang Tianjie Li Ting Pan Naru Zhao Yongchang Yao Zhichen Zhai Jiaan Zhou Chang Du Yingjun Wang | 2016 | Regenerative Biomaterials2016，3，5： | 1 |
| 16 | Th2 polarization in target organs is involved in the alleviation of pathological damage mediated by transplanting granulocyte colony-stimulating factor-primed donor T cells显示文摘Acute graft-versus-host disease(a GVHD)is caused by allo-activated donor T cells infiltrating target organs.As a regulator of immune function,granulocyte colony-stimulating factor(G-CSF)has been demonstrated to relieve the a GVHD reaction.However,the role of G-CSF-primed donor Tcells in specific target organs is still unknown.In this study,we employed a classical MHC-mismatched transplantation mouse model(C57BL/6 into BALB/c)and found that recipient mice transplanted with GCSF-primed T cells exhibited prolonged survival compared with that of the PBS-treated group.This protective function against GVHD mediated by G-CSF-primed donor T cells was further confirmed by decreased clinical and pathological scores in this a GVHD mouse model,especially in the lung and gut.Moreover,we found that Tcells polarized towards Th2 cells and regulatory T cells were increased in specific target organs.In addition,G-CSF treatment inhibited inducible co-stimulator(ICOS)expression and increased the expression of tolerance-related genes in recipient mice.Our study provides new insight into the immune regulatory effects of G-CSF on T cell-mediated a GVHD,especially for its precise regulation in GVHD target organs. | Yang Zhoui Leqing Cao Huidong Guo Yan Hong Ming Wang Ke Wang Xiaojun Huang Yingjun Chang | 2021 | Science China(Life Sciences)2021，64，7： | 1 |
| 17 | Early stage structural evolution of PLLA porous scaffolds in thermally induced phase separation process and the corresponding biodegradability and biological property显示文摘 | Jundong Shao Cong Chen Yingjun Wang Xiaofeng Chen Chang Du | 2012 | Polymer Degradation and Stability2012，，6： | 1 |
| 18 | PHBV microspheres - PLGA matrix composite scaffold for bone tissue engineering显示文摘 | Wei huang Xuetao Shi Li Ren Chang Du Yingjun Wang | 2010 | Biomaterials2010，31，15： | 1 |
| 19 | PHBV microspheres – PLGA matrix composite scaffold for bone tissue engineering显示文摘 | Wei Huang Xuetao Shi Li Ren Chang Du Yingjun Wang | 2010 | Biomaterials2010，，15： | 1 |
| 20 | MRD-directed and risk-adapted individualized stratified treatment of AML显示文摘Measurable residual disease(MRD)has been widely recognized as a biomarker for deeply evaluating complete remission(CR),predicting relapse,guiding pre-emptive interventions,and serving as an endpoint surrogate for drug testing.However,despite the emergence of new technologies,there remains a lack of comprehensive understanding regarding the proper techniques,sample materials,and optimal time points for MRD assessment.In this review,we summarized the MRD methods,sample sources,and evaluation frequency according to the risk category of the European Leukemia Net(ELN)2022.Additionally,we emphasize the importance of properly utilizing and combining these technologies.We have also refined the flowchart outlining each time point for preemptive interventions and intervention paths.The evaluation of MRD in acute myeloid leukemia(AML)is sophisticated,clinically applicable,and technology-dependent,and necessitates standardized approaches and further research. | Yijing Zhao Hanfei Guo Yingjun Chang | 2023 | Chinese Journal of Cancer Research2023，35，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员