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| 1 | m^6A-dependent biogenesis of circular RNAs in male germ cells显示文摘The majority of circular RNAs(circRNAs)spliced from coding genes contain open reading frames(ORFs)and thus,have protein coding potential.However,it remains unknown what regulates the biogenesis of these ORF-containing circRNAs,whether they are actually translated into proteins and what functions they play in specific physiological contexts.Here,we report that a large number of circRNAs are synthesized with increasing abundance when late pachytene spermatocytes develop into round and then elongating spermatids during murine spermatogenesis.For a subset of circRNAs,the back splicing appears to occur mostly at m^6A-enriched sites,which are usually located around the start and stop codons in linear mRNAs.Consequently,approximately a half of these male germ cell circRNAs contain large ORFs with m^6A-modified start codons in their junctions,features that have been recently shown to be associated with protein-coding potential.Hundreds of peptides encoded by the junction sequences of these circRNAs were detected using liquid chromatography coupled with mass spectrometry,suggesting that these circRNAs can indeed be translated into proteins in both developing(spermatocytes and spermatids)and mature(spermatozoa)male germ cells.The present study discovered not only a novel role of m^6A in the biogenesis of coding circRNAs,but also a potential mechanism to ensure stable and long-lasting protein production in the absence of linear mRNAs,i.e.,through production of circRNAs containing large ORFs and m^6A-modified start codons in junction sequences. | Chong Tang Yeming Xie Tian Yu Na Liu Zhuqing Wang Rebekah J.Woolsey Yunge Tang Xinzong Zhang Weibing Qin Ying Zhang Ge Song Weiwei Zheng Juan Wang Weitian Chen Xiongyi Wei Zhe Xie Rachel Klukovich Huili Zheng David R.Quilici Wei Yan | 2020 | Cell Research2020，30，3： | 12 |
| 2 | Variations in leaf litter decomposition across contrasting forest stands and controlling factors at local scale显示文摘Aims litter decomposition is a critical pathway linking the above-and belowground processes.However,factors underlying the local spatial variations in forest litter decomposition are still not fully addressed.We investigated leaf litter decomposition across con-trasting forest stands in central China,with objective to determine the spatial variations and controlling factors in forest floor leaf lit-ter decomposition in relation to changes in forest stands in a tem-perate forest ecosystem.Methods leaf litter decomposition was studied by using litterbag method across several typical forest stand types in baotianman Nature reserve,central China,including pure stands of Quercus aliena var.acuteserrata,Q.glandulifera var.brevipetiolata and Q.vari-abilis,respectively,and mixed pine/oak stands dominated by Pinus armandii and Q.aliena var.acuteserrata,as well as stands of pure Q.aliena var.acuteserrata trees ranging in stand age from~40 to>160 years.measurements were made on litter mass remaining and changes in litter chemistry during decomposition over a 2-year period,along with data collections on selective biotic and environmental factors.a reciprocal transplant experiment involv-ing Q.aliena var.acuteserrata and Q.variabilis was concurrently carried out to test the occurrence of‘home-field advantage(HFa)’in local forests when only considering contrasting oak tree spe-cies.Correlation analyses and path analyses were performed to identify the dominant drivers and their relative contributions to variations in leaf litter decomposition.Important findingssignificant variations were found in the rate of leaf litter decomposi-tion among stands of different tree species but not among stand age classes.The values of decay constant,k,varied from 0.62 in Q.aliena var.acuteserrata stands to 0.56 in Q.variabilis stands.The reciprocal litter transplant experiment showed that the rate of leaf litter decom-position was on average 5%slower in home-fields than on recipro-cal sites.Path analysis identified litter acid-unhydrolyzable residue(AUR)to N ratio,soil microbial biomass carbon(MBC),soil pH and soil organic carbon(SOC)as most prominent factors controlling the rate of leaf litter decomposition,collectively accounting for 57.8%of the variations;AUR/N had the greatest negative effect on k value,followed by weaker positive effects of SOC and MBC.our findings suggest that tree species plays a primary role in affecting forest floor leaf litter decomposition by determining the litter quality,with site environment being a secondary factor contributing to the local vari-ations in leaf litter decomposition in this temperate forest ecosystem. | Juan Wang Yeming You Zuoxin Tang Shirong Liu Osbert Jianxin Sun | 2015 | Journal of Plant Ecology2015，8，3： | 6 |
| 3 | Conversion of pure Chinese fir plantation to multi-layered mixed plantation enhances the soil aggregate stability by regulating microbial communities in subtropical China显示文摘Background:Soil aggregates are the basic units of soil structure,and their stability is a key indicator of soil quality and capacity to support ecosystem functions.The impacts of various environmental factors on soil aggregates have been widely studied.However,there remains elusive knowledge on the synergistic effects of changing forest stand structure on soil aggregate stability(SAS),particularly in subtropical China where soil erosion remains a critical issue.Methods:We investigated variations in the components of soil humus(HS),including humic acids(HAs),fulvic acids(FAs),and humins(HMs),under pure Chinese fir(Cunninghamia lanceolata)plantation(PP)and multilayered mixed plantation(MP)comprising C.lanceolata,Castanopsis hystrix,and Michelia hedyosperma.The state of soil aggregate stability,was determined by three separate methods,i.e.,dry-sieving,wet-sieving,and the Le Bissonnais.High-throughput sequencing was used to determine the diversity and composition of microbial communities under PP and MP.We then built partial least squares path models(PLS-PM)for assessing the responses of SAS to the variations in soil microorganisms and HS components.Results:The MP stands had significantly greater SAS(P<0.05),higher content of HAs and more rapid organic matter humification within aggregates,than the PP stands.High-throughput sequencing confirmed that the Pielou andα-diversity index values(Chao1 and Shannon)for fungi were all significantly higher under MP than under PP,while no marked difference was found in bacterialα-diversity between the two plantation types.Moreover,there were markedly greater abundance of three bacterial phyla(Verrucomicrobia,Chloroflexi,and Gemmatimonadetes)and three fungal phyla(Ascomycota,Kickxellomycota,and Glomeromycota),and significantly less abundance of two bacterial phyla(Planctomycetes and Firmicutes)and four fungal phyla(Basidiomycota,Mortierellomycota,Mucoromycota,and Rozellomycota)under MP than under PP.The Chloroflexi and Ascomycota phyla appeared to be the primary drivers of soil aggregate distribution.Our findings revealed that the promotion of SAS under MP was mainly driven by increased soil organic matter(SOM)content,which altered bacterial communities and enhanced fungal diversity,thereby increasing HAs content and the rate of organic matter humification.Conclusions:Considering the combined effects of enhanced soil quality,productivity,and relevant economic costs,introducing broadleaved tree species into Chinese fir plantations can be an effective strategy for stabilizing soil structure against erosion in subtropical China.Our study elucidated the controls on variations of SAS in Chinese fir-dominated plantations and demonstrated the benefit of converting pure Chinese fir plantation to multi-layered mixed plantations in increasing soil structural stability and improving site quality. | Guannv Gao Xueman Huang Haocheng Xu Yi Wang Weijun Shen Wen Zhang Jinliu Yan Xiaoyan Su Shushou Liao Yeming You | 2022 | Forest Ecosystems2022，9，6： | 4 |
| 4 | A comparison of decomposition dynamics among green tree leaves,partially decomposed tree leaf litter and their mixture in a warm temperate forest ecosystem显示文摘Decomposition dynamics were compared among green tree leaves,partially decomposed tree leaf litter(i.e.,decayed tree leaf litter on forest floor) and a mixture of the two in a warm temperate forest ecosystem in central China to test the influence of litter chemical quality on the degree of decomposition.The study was conducted in situ at two contrasting forest sites,an oak forest dominated by Quercus aliena var.acuteserrata Maxim.,and a mixed pine and oak forest dominated by Pinus armandii Franch.and Q.aliena var.acuteserrata.We found marked differences in the rate of decomposition among litter types at both forest sites;the litter decomposition constant,k,was about 39 % greater at the oak forest site and more than 70 % greater at the pine-oak forest site,for green leaves than for partially decomposed leaf litter.The decomposition dynamics and temporal changes in litter chemistry of the three litter types also greatly differed between the two forest sites.At both forest sites,the higher rate of decomposition for the green leaves was associated with a higher nitrogen(N) content and lower carbon to N ratio(C/N) and acid-unhydrolyzable residue to N ratio(AUR/N).We did not find any nonadditive effects when mixing green leaves and partially decomposed leaf litter.Our findings support the contention that litter chemical quality is one of the most important determinants of litter decomposition in forest ecosystems at the local or regional scale,but the effect of litter chemical quality on decomposition differs between the contrasting forest types and may vary with the stage of decomposition. | Juan Wang Yeming You Zuoxin Tang Xiaolu Sun Osbert Jianxin Sun | 2016 | Journal of Forestry Research2016，27，5： | 2 |
| 5 | Use of angiotensin-converting enzyme inhibitors and angiotensin Ⅱ receptor blockers in context of COVID-19 outbreak:a retrospective analysis显示文摘The possible effects of angiotensin-converting enzyme inhibitors(ACEIs)or angiotensin Ⅱ receptor blockers(ARBs)on COVID-19 disease severity have generated considerable debate.We performed a single-center,retrospective analysis of hospitalized adult COVID-19 patients in Wuhan,China,who had definite clinical outcome(dead or discharged)by February 15,2020.Patients on anti-hypertensive treatment with or without ACEI/ARB were compared on their clinical characteristics and outcomes.The medical records from 702 patients were screened.Among the 101 patients with a history of hypertension and taking at least one anti-hypertensive medication,40 patients were receiving ACEI/ARB as part of their regimen,and 61 patients were on antihypertensive medication other than ACEI/ARB.We observed no statistically significant differences in percentages of in-hospital mortality(28%vs.34%,P=0.46),ICU admission(20%vs.28%,P=0.37)or invasive mechanical ventilation(18%vs.26%,P=0.31)between patients with or without ACEI/ARB treatment.Further multivariable adjustment of age and gender did not provide evidence for a significant association between ACEI/ARB treatment and severe COVID-19 outcomes.Our findings confirm the lack of an association between chronic receipt of reninangiotensin system antagonists and severe outcomes of COVID-19.Patients should continue previous antihypertensive therapy until further evidence is available. | Jiuyang Xu Chaolin Huang Guohui Fan Zhibo Liu Lianhan Shang Fei Zhou Yeming Wang Jiapei Yu Luning Yang Ke Xie Zhisheng Huang Lixue Huang Xiaoying Gu Hui Li Yi Zhang Yimin Wang Frederick G.Hayden Peter W.Horby Bin Cao Chen Wang | 2020 | Frontiers of Medicine2020，14，5： | 2 |
| 6 | Pliocene charcoals from Shanxi Province of China and their application to studies of prehistoric wildfires显示文摘Charcoals collected from the middle-late Pliocene sediments of the Taigu Basin,Shanxi Province,China,have been identified as Ulmus sp.(Ulmaceae),Prunus sp.,Maloidoxylon sp.(Rosaceae),and Maclura sp.(Moraceae).These taxa,along with the previously known fossils,indicate the occurrence of temperate climate and local wildfire at that time.Charcoals of trees and/or shrubs and the morphological changes of these charcoals demonstrate that crown fires and surface fires occurred in the Taigu Basin during the middle-late Pliocene. | CHENG YeMing JIANG XiaoMei LI ChengSen WANG YuFei | 2011 | Science China Earth Sciences2011，54，4： | 1 |
| 7 | Phosphate and R2D2 Restrict the Substrate Specificity of Dicer-2, an ATP-Driven Ribonuclease显示文摘 | Elif Sarinay Cenik Ryuya Fukunaga Gang Lu Robert Dutcher Yeming Wang Traci M. Tanaka Hall Phillip D. Zamore | 2011 | Molecular Cell2011，，2： | 1 |
| 8 | Detection of cancerspecific markers amid massive spectral data 显示文摘 | Zhu Wei Wang Xuena Ma Yeming | 2003 | PNAS2003，100，14： | 1 |
| 9 | Current status and challenge of landslide moni toring in Three Gorge reservoir area, China显示文摘 | Zhang Yeming Peng Xuanming Wang Fawu Huo Zhitao Huang Bolin | 2004 | Landslides2004，，： | 1 |
| 10 | Expression of Human Calcitonin by Microencapsulated Recombinant Myoblasts显示文摘 | Yeming Wang Bingfang Zeng Xiaolin Li | 2006 | Biotechnology Letters2006，，18： | 1 |
| 11 | The July 14,2003 Qiangjiangping landslide Three Gorges Reservoir,China显示文摘 | Wang Fawu Zhang Yeming Huo Zhitao etc | 2004 | Landslide2004，2，： | 1 |
| 12 | Interaction Rule between Rare Earths and LowMelting Point Elements in Fe-,Cu-,AI-,Ni-Base Liquid Solutions显示文摘Interaction rule between representative RE and Sn,Sb,Pb.Cu,S,P low-melting point elements respectively in Fe-,Cu-,Al-,Ni-base liquid solutions including totally 34 ternary and quarternary systems was investigated.For each system some thermodynamic properties were obtained,such as the standard free energies of equilibrium reactions,activity interaction coefficients etc.. | Du Ting Sun Yunyong Wu Yeming Wang Longmei | 1997 | Journal of Iron and Steel Research(International)1997，4，1： | 1 |
| 13 | Research Progress of Anaerobic Digestion Pretreatment of Antibiotic Waste显示文摘Anaerobic digestion is one of the effective ways to dispose of antibiotic pharmaceutical waste. However,the inhibition of antibiotics on anaerobic fermentation microorganisms seriously hinders the normal physiological activities of anaerobic microorganisms and then affects the efficiency of anaerobic digestion. In order to solve this problem,related scholars have done a lot of research. It has been found that pretreatment of anaerobic microorganisms and antibiotic pharmaceutical waste can significantly improve the efficiency of anaerobic digestion. In this paper,the current feasible pretreatment methods were summarized,and the application of different pretreatment methods was analyzed to provide reference for improving pretreatment methods and improving anaerobic biological treatment ability of antibiotic waste. | Shengnan ZHAO Yanru CUI Hai GAO Yeming ZHAO Fengxian PANG Xiaodong CHENG Jiao XIE Xin WANG Wei OU | 2018 | Asian Agricultural Research2018，10，5： | 0 |
| 14 | Environmental Risk Assessment of Chemical Projects——A Case Study of a Polystyrene Production Project显示文摘Taking a polystyrene production project as an example,according to Technical Guidelines for Environmental Risk Assessment of Construction Projects( HJ /T169-2004),we firstly identify the risk of substances and production process to determine the major source of danger,and then conduct source analysis and consequence prediction of the maximum credible accident,finally assess the environmental risk of the project and propose main risk management measures. The results reveal that the environmental risk of the project is acceptable,and it is suggested that some risk prevention and mitigation measures as well as contingency plans should be established. | Yeming Li Xin Wang Tieli Xiang Wei Liu | 2013 | Meteorological and Environmental Research2013，4，10： | 0 |
| 15 | Construction of 3,9-diazatetraasteranes and 3,9-diazatetracyclododecanes by photocycloaddition reaction of 1,4-dihydropyridines:Experimental and theoretical investigation显示文摘A photocycloaddition reaction of ethyl 1,4-diaryl-1,4-dihydropyridine-3-carboxylate for the construction of 3,9-diazatetraasteranes(Pr)and 3,9-diazatetracyclododecanes(P2)is reported for the first time.The types of reaction product clearly differ with solvent,regardless of the irradiation wavelength.The difference in Pi and P2 lies in the second step of the intramolecular[2+2]photocyclization.In order to further investigate this phenomenon and gain a deeper understanding of the photochemical behavior of 1,4-dihydropyridines,DFT and TDDFT theoretical calculations are performed.The results provide a good explanation for the formation of 3,9-diazatetraasteranes and 3,9-diazatetracyclododecanes. | Runzhi Sun Xiuqing Song Shijie Wang Xiaokun Zhang Hong Yan Yeming Wang | 2023 | Chinese Chemical Letters2023，34，12： | 0 |
| 16 | Cerebral ischemic injury after transcatheter aortic valve replacement in patients with pure aortic regurgitation显示文摘Considering the surgical risk stratification for patients with severe calcific aortic stenosis(AS),transcatheter aortic valve replacement(TAVR)is a reliable alternative to surgical aortic valve replacement(SAVR)(Fan et al.,2020,2021;Lee et al.,2021).Despite the favorable clinical benefits of TAVR,stroke remains a dreaded perioperative complication(Auffret et al.,2016;Kapadia et al.,2016;Kleiman et al.,2016;Huded et al.,2019).Ischemic overt stroke,identified in 1.4%to 4.3%of patients in TAVR clinical practice,has been associated with prolonged disability and increased mortality(Auffret et al.,2016;Kapadia et al.,2016;Levi et al.,2022).The prevalence of hyperintensity cerebral ischemic lesions detected by diffusion-weighted magnetic resonance imaging(DW-MRI)was reported to be about 80%,which is associated with impaired neurocognitive function and vascular dementia(Vermeer et al.,2003;Barber et al.,2008;Kahlert et al.,2010). | Xianbao LIU Hanyi DAI Jiaqi FAN Dao ZHOU Gangjie ZHU Abuduwufuer YIDILISI Jun CHEN Yeming XU Lihan WANG Jian'an WANG | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，6： | 0 |
| 17 | Impact of corticosteroids on initiation and half-year durability of humoral response in COVID-19 survivors显示文摘Background:The impact of corticosteroids on humoral responses in coronavirus disease 2019(COVID-19)sur-vivors during the acute phase and subsequent 6-month period remains unknown.This study aimed to determine how the use of corticosteroids influences the initiation and duration of humoral responses in COVID-19 survivors 6 months after infection onset.Methods:We used kinetic antibody data from the lopinavir-ritonavir trial conducted at Jin Yin-Tan Hospital in January 2020,which involved adults hospitalized with severe COVID-19(LOTUS,ChiCTR2000029308).Anti-body samples were collected from 192 patients during hospitalization,and kinetic antibodies were monitored at all available time points after recruitment.Additionally,plasma samples were collected from 101 COVID-19 survivors for comprehensive humoral immune measurement at the half-year follow-up visit.The main focus was comparing the humoral responses between patients treated with systemic corticosteroid therapy and the non-corticosteroid group.Results:From illness onset to day 30,the median antibody titre areas under the receiver operating characteristic curve(AUCs)of nucleoprotein(N),spike protein(S),and receptor-binding domain(RBD)immunoglobulin G(IgG)were significantly lower in the corticosteroids group.The AUCs of N-,S-,and RBD-IgM as well as neutralizing antibodies(NAbs)were numerically lower in the corticosteroids group compared with the non-corticosteroid group.However,peak titres of N,S,RBD-IgM and-IgG and NAbs were not influenced by corticosteroids.During 6-month follow-up,we observed a delayed decline for most binding antibodies,except N-IgM(��−0.05,95%CI[−0.10,0.00])in the corticosteroids group,though not reaching statistical significance.No significant difference was observed for NAbs.However,for the half-year seropositive rate,corticosteroids significantly accelerated the decay of IgA and IgM but made no difference to N-,S-,and RBD-IgG or NAbs.Additionally,corticosteroids group showed a trend towards delayed viral clearance compared with the non-corticosteroid group,but the results were not statistically significant(adjusted hazard ratio 0.71,95%CI 0.50-1.00;P=0.0508).Conclusion:Our findings suggested that corticosteroid therapy was associated with impaired initiation of the antibody response but this did not compromise the peak titres of binding and neutralizing antibodies.Throughout the decay phase,from the acute phase to the half-year follow-up visit,short-term and low-dose corticosteroids did not significantly affect humoral responses,except for accelerating the waning of short-lived antibodies. | Yeming Wang Li Guo Guohui Fan Yang Han Qiao Zhang Lili Ren Hui Zhang Geng Wang Xueyang Zhang Tingxuan Huang Weiyang Wang Lan Chen Lixue Huang Xiaoying Gu Xinming Wang Jingchuan Zhong Ying Wang Hui Li Jiapei Yu Zhibo Liu Chaolin Huang Bin Cao Jianwei Wang | 2024 | Chinese Medical Journal Pulmonary and Critical Care Medicine2024，2，1： | 0 |
| 18 | Quantitative Analysis of Kruppel‑Like Factor 5‑Related Messenger RNA Transcripts in Ischemic Myocardium for Discrimination of Death Causes显示文摘Background:Accumulated studies have demonstrated that Kruppel‑like factor 5(KLF5),a transcription factor,plays an important role in regulating cell proliferation and tissue remodeling through the expression of its downstream genes.KLF5‑related factors are expected to be involved in the healing process after myocardial injury or myocardial ischemic changes,especially for the forensic diagnosis of myocardial ischemic physiopathology.Aim and Objectives:This study aimed to explore the discrimination ability and applicability of KLF5-related factors in SCD caused by MI compared with other causes of death to provide further insights into the forensic diagnosis of myocardial ischemic pathology.Materials and Methods:The relative quantification of F‑Box and WD Repeat Domain Containing 7(FBW7),KLF5,factor‑binding protein(FGFBP)1,and FGFBP2 messenger RNAs(mRNAs)in myocardial tissue samples was performed using real‑time fluorescence quantitative reverse transcription polymerase chain reaction.KLF5 and FGFBP1/2 protein levels were examined using immunohistochemistry(IHC).The forensic autopsy cases(27 in total,autopsy within 72 h postmortem)included seven cases of acute myocardial infarction and 10 cases of acute myocardial ischemia.There were 10 cases in the control group,including four cases of traffic injury one case of injury by fall from height,one case of electric death,and four cases of blunt force injury.Results:Characteristic results were found in myocardial samples from three groups of deaths:KLF5 and FGFBP1 mRNA levels were significantly elevated in the infarction and ischemia groups,while FBW7 mRNA levels were significantly decreased.FBW7 is an important ubiquitin ligase that can mediate the degradation of KLF5 protein.In addition,FBW7 and FGFBP2 mRNA levels were decreased in the infarction group compared with the ischemia group.The IHC results were consistent with the observed mRNA expression patterns.Conclusions:Quantitative detection of FBW7,KLF5,FGFBP1,and FGFBP2 mRNA transcripts in myocardial tissues supports the pathophysiological study of myocardial ischemic diseases and provides molecular pathological evidence for forensic discrimination of death causes. | Xingyu Ma Yeming Li Ya Xi Liyang Su Yuxing Tong Chun Wang Tianshui Yu Dong Zhao | 2022 | Journal of Forensic Science and Medicine2022，8，4： | 0 |
| 19 | Long-term outcomes in COVID-19 patients who recovered from the first wave of the pandemic显示文摘This cross-sectional study evaluated the long-term health effects of coronavirus disease 2019(COVID-19)in Jianghan District(Wuhan,China).The results showed that 61.4%of COVID-19 patients reported at least one symptom and 8.8%had depressive symptoms at the 17-month follow-up.The proportion of patients with chest radiographic abnormalities in Fangcang shelter hospitals and designated COVID-19hospitals was 31.6%and 41.1%,respectively,and the proportion of patients with impaired pulmonary diffusion capacity in these hospitals was 52.8%and 60.9%,respectively.Female sex(odds ratio[OR]=1.48,95%confidence interval[CI]:1.16–1.88),severe disease(OR=1.46,95%CI:1.01–2.10)and a higher number of initial symptoms(OR=1.31,95%CI:1.23–1.40)were associated with the development of sequelae symptoms at 17 months.This study involving community-dwelling COVID-19adults may help determine the long-term effects of COVID-19 during the first pandemic wave.Nonetheless,larger follow-up studies are needed to characterize the post-COVID-19 condition. | Dan Cui Simiao Chen Luzhao Feng Mengmeng Jia Yeming Wang Weijun Xiao Yanxia Sun Qiangru Huang Libing Ma Zhiwei Leng Hao Wang Bin Cao Weizhong Yang Juntao Yang Chen Wang | 2022 | National Science Review2022，9，11： | 0 |
| 20 | Thermodynamic Behaviour of Fe-S-RE,Fe-Csat.-S-RE,Ni-S-RE,Cu-S-RE Solutions显示文摘The temperature dependences of the equilibrium constants for the reactions RES(S)=[RE]+[S].(RE=Ce,La,Nd,Sm,Y),of the standard Gibbs energies for the RES formed and of the interactioncoefficients between RE elements and S for the Fe-base,Ni-base and Cu-base solutions are sum-marized. | Ting DU Longmei WANG Yeming WU Yuqing ZHANG Aisheng LIU Central Iron and Steel Research Institute,Beijing,100081,China | 1993 | Journal of Materials Science & Technology1993，9，1： | 0 |
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