|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Loss of YTHDF2-mediated m6A-dependent mRNA clearance facilitates hematopoietic stem cell regeneration显示文摘 | Hu Wang Hongna Zuo Jin Liu Fei Wen Yawei Gao Xudong Zhu Bo Liu Feng Xiao Wengong Wang Gang Huang Bin Shen Zhenyu JU | 2018 | Cell Research2018，28，10： | 16 |
| 2 | Geochemistry and Zircon U-Pb Dating of Changshagou Adakite from the South Altyn UHPM Terrane: Evidence of the Partial Melting of the Lower Crust显示文摘Changshagou adakite, an outcrop in the middle segment of the South Altyn Tagh ultra-high pressure metamorphism(UHPM) terrane, contains medium-K cal-alkaline and weakly peraluminous compositions(Si O2 = 66.79% to 68.65%, Al2O3 = 17.48% to 18.31%, K2 O + Na2 O = 6.32% to 6.88%, K2O/Na2 O = 0.25 to 0.33, A/CNK = 1.01 to 1.06). This outcrop is also enriched with large ion lithophile elements but with depleted high-field strength elements(HFSE) showing clearly negative Nb, Ta, and Ti anomalies. REE distribution patterns show a positive anomaly of Eu(δEu = 1.15 to 1.31) and weakly enriched with LREE compared with HREE(LREE/HREE = 1.02 to 4.20). Experimental results and several characteristics, including relatively low Nb/Ta ratios(6.03 to 8.45) and high Sr, Sr/Y,(La/Yb)N and low Y and Yb, which indicate the presence of residual garnet and the absence of plagioclase in the source region, show that adakite may form at a pressure ranging from 1.2 GPa to 1.5 GPa and at a temperature of approximately 900°C. Low Cr, Ni, and Mg# values, trace element patterns, and Si O2–Mg# and Si O2–Mg O diagrams indicate that rocks are formed by the partial melting of a thickened lower continental crust. LA-ICP-MS in situ U-Pb dating yields two group ages: 503.1 ± 1.7 Ma(core) and 453.1 ± 3.0 Ma(rim). The Th/U ratios of the core and the rim are 0.11 to 0.40 and 0.03 to 0.07, respectively. Considering the zircon CL image characteristics, Th/U ratios, and previous studies on regional UHPM rocks, adakite formed at 503.1 ± 1.7 Ma and underwent a tectothermal event as a result of the break-off of the Altyn deep subducted continental crust at 453.1 ± 3.0 Ma. | KANG Lei LIU Liang WANG Chao CAO Yuting YANG Wenqiang WANG Yawei LIAO Xiaoying | 2014 | Acta Geologica Sinica(English Edition)2014，88，5： | 15 |
| 3 | AOX contamination status and genotoxicity of AOX-bearing pharmaceutical wastewater显示文摘Adsorbable organic halogens(AOX) are a general indicator for the total amount of compounds containing organically bonded halogens. AOX concentrations and components were investigated along the wastewater treatment process in four large-scale pharmaceutical factories of China, and genotoxicity based on the SOS/umu test was also evaluated. The results showed that AOX concentrations in wastewater of four factories ranged from 4.6 to 619.4 mg/L, which were high but greatly different owing to differences in the raw materials and products. The wastewater treatment process removed 50.0%–89.9% of AOX, leaving 1.3–302.5 mg/L AOX in the effluents. Genotoxicity levels ranged between 2.1 and 68.0 μg 4-NQO/L in the raw wastewater and decreased to 1.2–41.2 μg 4-NQO/L in the effluents of the wastewater treatment plants(WWTPs). One of the main products of factory I, ciprofloxacin, was identified as the predominant contributor to its genotoxicity. However, for the other three factories, no significant relationship was observed between genotoxicity and detected AOX compounds. | Yawei Xie Lujun Chen Rui Liu | 2017 | Journal of Environmental Sciences2017，29，2： | 15 |
| 4 | Single-cell RNA-seq reveals the diversity of trophoblast subtypes and patterns of differentiation in the human placenta显示文摘胎盘为成功的怀孕和胎儿和怀孕女人的健康是关键的。然而,人的 trophoblast 系怎么被调整,包括胎盘的房间的分类,子类型糟糕被理解。这里,我们在排序的胎盘的房间上执行了定序的单个房间的 RNA (RNA-seq ) 从第一三个月并且秒三个月人胎盘。已知的 cytotrophoblast 房间(CTB ) 的房间的新子类型, extravillous trophoblast 房间(EVT ) , Hofbauer 房间,和间充质的 stromal 房间被识别, cell-type-specific 基因签名被定义。机能上地,这研究揭示了人的胎盘的许多以前未知的功能。尤其是, 102 多肽荷尔蒙基因被发现被胎盘的房间的各种各样的子类型表示,它在调整母亲的生理学的胎儿的生长和改编到怀孕建议建筑群和这些荷尔蒙的重要角色。这些结果在单个房间的分辨率记录人的胎盘的 trophoblast 区别并且因此在怀孕的早阶段期间推进我们人的胎座式的理解。 | Yawei Liu Xiaoying Fan Rui Wang Xiaoyin Lu Yan-Li Dang Huiying Wang Hai-Yan Lin Cheng Zhu Hao Ge James C. Cross Hongmei Wang | 2018 | Cell Research2018，28，8： | 15 |
| 5 | Recent developments in nanofiltration membranes based on nanomaterials显示文摘Nanofiltration membranes are the core elements for nanofiltration process. The chemical structures and physical properties of nanofiltration membranes determine water permeability, solute selectivity, mechanical/thermal stability, and antifouling properties, which greatly influence the separation efficiency and operation cost in nanofiltration applications. In recent years, a great progress has been made in the development of high performance nanofiltration membranes based on nanomaterials. Considering the increasing interest in this field, this paper reviews the recent studies on the nanofiltration membranes comprising various nanomaterials, including the metal and metal oxide nanoparticles, carbon-based nanomaterials, metal–organic frameworks(MOFs), water channel proteins, and organic micro/nanoparticles. Finally, a perspective is given on the further exploitation of advanced nanomaterials and novel strategy for fabricating nano-based nanofiltration membranes. Moreover,the development of precision instruments and simulation techniques is necessary for the characterization of membrane microstructure and investigation of the separation and antifouling mechanism of nanofiltration membranes prepared with nanomaterials. | Yanli Ji Weijie Qian Yawei Yu Quanfu An Lifen Liu Yong Zhou Congjie Gao | 2017 | Chinese Journal of Chemical Engineering2017，25，11： | 12 |
| 6 | Carbon pools and fluxes in the China Seas and adjacent oceans显示文摘The China Seas include the South China Sea, East China Sea, Yellow Sea, and Bohai Sea. Located off the Northwestern Pacific margin, covering 4700000 km^2 from tropical to northern temperate zones, and including a variety of continental margins/basins and depths, the China Seas provide typical cases for carbon budget studies. The South China Sea being a deep basin and part of the Western Pacific Warm Pool is characterized by oceanic features; the East China Sea with a wide continental shelf, enormous terrestrial discharges and open margins to the West Pacific, is featured by strong cross-shelf materials transport; the Yellow Sea is featured by the confluence of cold and warm waters; and the Bohai Sea is a shallow semiclosed gulf with strong impacts of human activities. Three large rivers, the Yangtze River, Yellow River, and Pearl River, flow into the East China Sea, the Bohai Sea, and the South China Sea, respectively. The Kuroshio Current at the outer margin of the Chinese continental shelf is one of the two major western boundary currents of the world oceans and its strength and position directly affect the regional climate of China. These characteristics make the China Seas a typical case of marginal seas to study carbon storage and fluxes. This paper systematically analyzes the literature data on the carbon pools and fluxes of the Bohai Sea,Yellow Sea, East China Sea, and South China Sea, including different interfaces(land-sea, sea-air, sediment-water, and marginal sea-open ocean) and different ecosystems(mangroves, wetland, seagrass beds, macroalgae mariculture, coral reefs, euphotic zones, and water column). Among the four seas, the Bohai Sea and South China Sea are acting as CO_2 sources, releasing about0.22 and 13.86–33.60 Tg C yr^(-1) into the atmosphere, respectively, whereas the Yellow Sea and East China Sea are acting as carbon sinks, absorbing about 1.15 and 6.92–23.30 Tg C yr^(-1) of atmospheric CO_2, respectively. Overall, if only the CO_2 exchange at the sea-air interface is considered, the Chinese marginal seas appear to be a source of atmospheric CO_2, with a net release of 6.01–9.33 Tg C yr^(-1), mainly from the inputs of rivers and adjacent oceans. The riverine dissolved inorganic carbon (DIC) input into the Bohai Sea and Yellow Sea, East China Sea, and South China Sea are 5.04, 14.60, and 40.14 Tg C yr^(-1),respectively. The DIC input from adjacent oceans is as high as 144.81 Tg C yr^(-1), significantly exceeding the carbon released from the seas to the atmosphere. In terms of output, the depositional fluxes of organic carbon in the Bohai Sea, Yellow Sea, East China Sea, and South China Sea are 2.00, 3.60, 7.40, and 5.92 Tg C yr^(-1), respectively. The fluxes of organic carbon from the East China Sea and South China Sea to the adjacent oceans are 15.25–36.70 and 43.93 Tg C yr^(-1), respectively. The annual carbon storage of mangroves, wetlands, and seagrass in Chinese coastal waters is 0.36–1.75 Tg C yr^(-1), with a dissolved organic carbon(DOC) output from seagrass beds of up to 0.59 Tg C yr^(-1). Removable organic carbon flux by Chinese macroalgae mariculture account for 0.68 Tg C yr^(-1) and the associated POC depositional and DOC releasing fluxes are 0.14 and 0.82 Tg C yr^(-1), respectively. Thus, in total, the annual output of organic carbon, which is mainly DOC, in the China Seas is 81.72–104.56 Tg C yr^(-1). The DOC efflux from the East China Sea to the adjacent oceans is 15.00–35.00 Tg C yr^(-1). The DOC efflux from the South China Sea is 31.39 Tg C yr^(-1). Although the marginal China Seas seem to be a source of atmospheric CO_2 based on the CO_2 flux at the sea-air interface, the combined effects of the riverine input in the area, oceanic input, depositional export,and microbial carbon pump(DOC conversion and output) indicate that the China Seas represent an important carbon storage area. | Nianzhi JIAO Yantao LIANG Yongyu ZHANG Jihua LIU Yao ZHANG Rui ZHANG Meixun ZHAO Minhan DAI Weidong ZHAI Kunshan GAO Jinming SONG Dongliang YUAN Chao LI Guanghui LIN Xiaoping HUANG Hongqiang YAN Limin HU Zenghu ZHANG Long WANG Chunjie CAO Yawei LUO Tingwei LUO Nannan WANG Hongyue DANG Dongxiao WANG Si ZHANG | 2018 | Science China Earth Sciences2018，61，11： | 12 |
| 7 | Development of a static test apparatus for evaluating the performance of three PM2.5 separators commonly used in China显示文摘PM2.5 separator directly affects the accuracy of PM2.5 sampling.The specification testing and evaluation for PM2.5 separator is particularly important,especially under China’s wide variation of terrain and climate.In this study,first a static test apparatus based on polydisperse aerosol was established and calibrated to evaluate the performance of the PM2.5 separators.A uniform mixing chamber was developed to make particles mix completely.The aerosol concentration relative standard deviations of three test points at the same horizontal chamber position were less than 0.57%,and the particle size distribution obeyed logarithmic normal distribution with an R2 of 0.996.The flow rate deviation between the measurement and the set point flow rate agreed to within±1.0%in the range of-40 to 50℃.Secondly,the separation,flow and loading characteristics of three cyclone separators(VSCC-A,SCC-A and SCC112)were evaluated using this system.The results showed that the 50%cutoff sizes(D50)of the three cyclones were 2.48,2.47 and 2.44μm when worked at the manufacturer’s recommended flow rates,respectively.The geometric standard deviation(GSD)of the capture efficiency of VSCCA was 1.23,showed a slightly sharper than SCC-A(GSD=1.27),while the SCC112 did not meet the relevant indicator(GSD=1.2±0.1)with a GSD=1.44.The flow rate and loading test had a great effect on D50,while the GSD remained almost the same as before.In addition,the maintenance frequency under different air pollution conditions of the cyclones was summarized according to the loading test. | Peng Du Jianguo Liu Huaqiao Gui Jiaoshi Zhang Tongzhu Yu Jie Wang Yin Cheng Yihuai Lu Yawei Yao Qiang Fu ChihChieh Chen | 2020 | Journal of Environmental Sciences2020，32，1： | 9 |
| 8 | Primarily screening and analyzing ESTs differentially expressed in rats' primary liver cancer显示文摘Objective: To screen and analyze key express sequence tags (ESTs) which were differentially displayed in every period of SD rats' primary hepatic carcinoma and reveal the molecular mechanism of carcinogenesis. Methods: Using diethylnitrosamine (DENA) as a cancerigenic agent, animal models with different phases of primary hepatic cancer were constructed in SD rats. Rats were respectively sacrificed at d 14, d 28, d 56, d 77, d 105 and d 112 after the rats received DENA by gavage, then the livers were harvested. One part of the livers was classified according to their pathological changes, while the other was reserved for molecular mechanism studies on hepatocarcinogenesis. The differentially expressed genes were isolated from both normal and morbid tissues by mRNA differential display technique (DDRT-PCR). After the fragments were sequenced, bioinformatics were used to analyze the results. Results: Twelve differentially expressed cDNA fragments were obtained. Nine fragments had the homology with known cDNA clones, especially EST-7 was similar to BN/SsNHsdMCW mitochondrion gene and the identity was 100% which suggested EST-7 may be the part of BN/SsNHsdMCW mitochondrion gene. In contrast, other three fragments (EST-1, EST-3 and EST-5) had extremely low identity to any genes registered in GENBANK databases. Conclusions: BN/SsNHsdMCW mitochondrion gene was expressed in different periods of hepatocarcinogenesis. Moreover, EST-1, EST-3 and EST-5 were suggested to contribute to the development of rat hepatocarcinogenesis, and thus may be candidates of new targets of oncogenes or cancer suppressor genes. | Dandan Liu Lijuan Zhi Mingxia Ma Dan Qiao Meijuan Wang Yawei Wang Baijie Jin Anqi Li Guting Liu Yiqing Zhang Yanyan Song Hongxu Zhang | 2013 | Chinese Journal of Cancer Research2013，25，1： | 9 |
| 9 | Ce and Er Co-doped TiO2 for rapid bacteria- killing using visible light显示文摘Bacterial infection and related diseases are threatening the health of human beings.Photocatalytic disinfection as a simple and low-cost disinfection strategy is attracting more and more attention.In this work,TiO2 nanoparticles(NPs)were modified by co-doping of Ce and Er using the sol-gel method,which endowed TiO2 NPs with enhanced visible light photocatalytic performance but not pure ultraviolet photocatalytic properties compared the untreated TiO2.Our results disclosed that as the doping content of Er increased,the photocatalytic activity of modified TiO2 NPs initially increased and subsequently decreased.The same trend occurred for Ce doping.When the doping dose of Er and Ce is 0.5 mol%and 0.2 mol%,the 0.5Ce0.2Ti-O calcined at 800℃ presented the best antibacterial properties,with the antibacterial efficiency of 91.23%and 92.8%for Staphylococcus aureus and Escherichia coli,respectively.The existence of Er ions is thought to successfully turn the near-infrared radiation into visible region,which is easier to be absorbed by TiO2 NPs.Meanwhile,the addition of Ce ions can effectively extend spectral response range and inhibit the recombination of electrons and holes,enhancing the photocatalytic disinfection activity of co-doped TiO2. | Yawei Ren Yajing Han Zhaoyang Li Xiangmei Liu Shengli Zhu Yanqin Liang Kelvin Wai Kwok Yeung Shuilin Wu | 2020 | Bioactive Materials2020，5，2： | 7 |
| 10 | Remaining Useful Life Model and Assessment of Mechanical Products: A Brief Review and a Note on the State Space Model Method显示文摘The remaining useful life(RUL) prediction of mechanical products has been widely studied for online system performance reliability, device remanufacturing, and product safety(safety awareness and safety improvement). These studies incorporated many di erent models, algorithms, and techniques for modeling and assessment. In this paper, methods of RUL assessment are summarized and expounded upon using two major methods: physics model based and data driven based methods. The advantages and disadvantages of each of these methods are deliberated and compared as well. Due to the intricacy of failure mechanism in system, and di culty in physics degradation observation, RUL assessment based on observations of performance variables turns into a science in evaluating the degradation. A modeling method from control systems, the state space model(SSM), as a first order hidden Markov, is presented. In the context of non-linear and non-Gaussian systems, the SSM methodology is capable of performing remaining life assessment by using Bayesian estimation(sequential Monte Carlo). Being e ective for non-linear and non-Gaussian dynamics, the methodology can perform the assessment recursively online for applications in CBM(condition based maintenance), PHM(prognostics and health management), remanufacturing, and system performance reliability. Finally, the discussion raises concerns regarding online sensing data for SSM modeling and assessment of RUL. | Yawei Hu Shujie Liu Huitian Lu Hongchao Zhang | 2019 | Chinese Journal of Mechanical Engineering2019，32，1： | 6 |
| 11 | Effects of chlortetracycline,Cu and their combination on the performance and microbial community dynamics in swine manure anaerobic digestion显示文摘Swine manure was typical for the combined pollution of heavy metals and antibiotics. The effects of widely used veterinary antibiotic chlortetracycline(CTC), Cu and their combination on swine manure anaerobic digestion performance and microbial community have never been investigated. Thus, four 2 L anaerobic digestion reactors were established including reactor A(control), B(CTC spiked by 0.5 g/kg dry weight, dw), C(Cu spiked by 5 g/kg dw) and D(combination of CTC, 0.5 g/kg dw, and Cu, 5 g/kg dw), and dynamics of bacterial and archaeal community structure was investigated using high throughput sequencing method. Results showed that addition of CTC and Cu separately could increase the total biogas production by21.6% and 15.8%, respectively, while combination of CTC and Cu severely inhibited anaerobic digestion(by 30.3%). Furthermore, corresponding to different stages and reactors, four kinds of microbes including bacteria and archaea were described in detail, and the effects of CTC, Cu and their combination mainly occurred at hydrolysis and acidification phases. The addition of Cu alone changed the dynamics of archaeal community significantly. It was genus Methanomassiliicoccus that dominated at the active methane production for A, B and D, while it was genus Methanobrevibacter and Methanoculleus for C. | Rui Wang Junya Zhang Jibao Liu Dawei Yu Hui Zhong Yawei Wang Meixue Chen Juan Tong Yuansong Wei | 2018 | Journal of Environmental Sciences2018，30，5： | 5 |
| 12 | Nuclear m^(6)A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos显示文摘N^(6)-methyladenosine(m^(6)A)on chromosome-associated regulatory RNAs(carRNAs),including repeat RNAs,plays important roles in tuning the chromatin state and transcription,but the intrinsic mechanism remains unclear.Here,we report that YTHDC1 plays indispensable roles in the self-renewal and differentiation potency of mouse embryonic stem cells(ESCs),which highly depends on the m^(6)A-binding ability.Ythdcl is required for sufficient rRNA synthesis and repression of the 2-cell(2C)transcriptional program in ESCs,which recapitulates the transcriptome regulation by the LINE1 scaffold.Detailed analyses revealed that YTHDC1 recognizes m^(6)A on LINE1 RNAs in the nucleus and regulates the formation of the LINE1-NCL partnership and the chromatin recruitment of KAP1.Moreover,the establishment of H3K9me3 on 2C-related retrotrans-posons is interrupted in Ythdcl-depleted ESCs and inner cell mass(ICM)cells,which consequently increases the transcriptional activities.Our study reveals a role of m^(6)A in regulating the RNA scaffold,providing a new model for the RNA-chromatin cross-talk. | Chuan Chen Wenqiang Liu Jiayin Guo Yuanyuan Liu Xuelian Liu Jun Liu Xiaoyang Dou Rongrong Le Yixin Huang Chong Li Lingyue Yang Xiaochen Kou Yanhong Zhao You Wu Jiayu Chen Hong Wang Bin Shen Yawei Gao Shaorong Gao | 2021 | Protein & Cell2021，12，6： | 4 |
| 13 | Development of matrix solid-phase dispersion method for the extraction of short-chain chlorinated paraffins in human placenta显示文摘Chlorinated paraffins(SCCPs) are widely used worldwide, and they can be released into the environment during their production, transport, usage and disposal, which pose potential risks for human health. In this work, an efficient, reliable and rapid pretreatment method based on matrix solid-phase dispersion(MSPD) was developed for the analysis of short-chain CPs(SCCPs) in human placenta by gas chromatograph-electron capture negative ion low-resolution mass spectrometry(GC-ECNI-LRMS) and gas chromatography–quadrupole time-of-flight mass spectrometry(GC–QTOF-HRMS). The MSPD-relevant parameters including dispersing sorbent,sample-to-sorbent mass ratio, and elution solvent were optimized using the orthogonal test.Silica gel was found to be the optimal dispersing sorbent among the selected matrices. Under the optimal conditions, 44% acidic silica gel can be used as the co-sorbent to remove lipid and eluted by the mixture of hexane and dichloromethane(7:3, V/V). The spiked recoveries of the optimized method were 77.4% and 91.4% for analyzing SCCPs in human placenta by GC-ECNI-LRMS and GC–QTOF-HRMS, and the corresponding relative standard deviations were10.2% and 5.6%, respectively. The method detection limit for the total SCCPs was 36.8 ng/g(dry weight, dw) and 19.2 ng/g(dw) as measured by GC-ECNI-LRMS and GC–QTOF-HRMS,respectively. The concentrations of SCCPs in four human placentas were in the range of | Ying Wang Wei Gao Jing Wu Huijin Liu Yingjun Wang Yawei Wang Guibin Jiang | 2017 | Journal of Environmental Sciences2017，29，12： | 4 |
| 14 | Exosomes Released from Rabies Virus-Infected Cells May be Involved in the Infection Process显示文摘Exosomes are cell-derived vesicles that are secreted by many eukaryotic cells. It has recently attracted attention as vehicles of intercellular communication. Virus-infected cells release exosomes, which contain viral proteins, RNA, and pathogenic molecules. However, the role of exosomes in virus infection process remains unclear and needs to be further investigated.In this study, we aimed to evaluate the effects of exosomes on rabies virus infection. OptiPrep^(TM) density gradient centrifugation was used to isolate exosomes from rabies virus-infected cell culture supernatants. A rabies virus G protein enzyme-linked immunosorbent assay and acetylcholinesterase activity assays were performed to verify the centrifugation fractions. Exosomes were then characterized using transmission electron microscopy and Western blotting. Our results showed that rabies virus infection increased the release of exosomes. Treatment with GW4869 and si-Rab27 a, two exosomal secretion inhibitors, inhibited exosome release. Furthermore, the inhibitors reduced the levels of extracellular and intracellular viral RNA. These data indicated that exosomes may participate in the viral infection process. Moreover, our results establish a basis for future research into the roles of exosomes in rabies virus infection and as potential targets for developing new antiviral strategies. | Jingyu Wang Fan Wu Chuntian Liu Wenwen Dai Yawei Teng Weiheng Su Wei Kong Feng Gao Linjun Cai Ali Hou Chunlai Jiang | 2019 | Virologica Sinica2019，34，1： | 3 |
| 15 | Ultralight and ordered lamellar polyimide-based graphene foams with efficient broadband electromagnetic absorption显示文摘The development of high-performance microwave absorption materials with strong absorption capacity and broad bandwidth is highly desirable in the field of electromagnetic pollution protection.Herein,ultralight polyimide-based graphene foam with ordered lamellar structure is precisely designed and controllably constructed by bidirectional freezing process.More lamellar interfaces formed inside the foam per unit volume effectively facilitate the layer-by-layer dissipation for the vertical incident electromagnetic waves,thereby endowing the foam with efficient broadband electromagnetic absorption performance.More importantly,electromagnetic absorption performance can be controllably adjusted by optimizing impedance distribution and microstructure of skeletons.As a result,the optimized foam with an ultralow density of 9.10 mg/cm^(3)presents a minimum reflection loss value of-61.29 dB at 9.25 GHz and an effective absorption bandwidth of 5.51 GHz(7.06-12.57 GHz,covering the whole X band) when the thickness is 4.75 mm. | Yawei Zhang Shuangshuang Li Xinwei Tang Wei Fan Qianqian Lan Le Li Piming Ma Weifu Dong Zicheng Wang Tianxi Liu | 2022 | Journal of Materials Science & Technology2022，，7： | 3 |
| 16 | Identification of novel Lynch syndrome mutations in Chinese patients with endometriod endometrial cancer显示文摘Objective:Lynch syndrome(LS)predisposes patients to early onset endometrioid endometrial cancer(EEC).However,little is known about LS-related EEC in the Chinese population.The aim of this study was to investigate the prevalence of LS and to identify the specific variants of LS in Chinese patients with EEC.Methods:We applied universal immunohistochemistry screening to detect the expression of mismatch repair(MMR)proteins,which was followed by MLH1 methylation analysis to identify suspected LS cases,next-generation sequencing(NGS)to confirm LS,and microsatellite instability(MSI)analysis to verify LS.Results:We collected 211 samples with EEC.Twenty-seven(27/211,12.8%)EEC cases had a loss of MMR protein expression.After MLH1 methylation analysis,16 EEC cases were suggested to be associated with LS.Finally,through NGS and MSI analysis,we determined that 10 EEC(10/209,4.78%)cases were associated with LS.Among those cases,3 unreported mutations(1 frameshift and 2 nonsense)were identified.M SH6 c.597_597delC,found in 4 patients,is likely to be a founder mutation in China.Conclusions:We demonstrated the feasibility of a process for LS screening in Chinese patients with EEC,by using universal immunohistochemistry screening followed by MLH1 methylation analysis and confirmation through NGS and MSI analysis.The novel mutations identified in this study expand knowledge of LS. | Caixia Ren Yan Liu Yuxiang Wang Yan Tang Yawei Wei Congrong Liu Hongquan Zhang | 2020 | Cancer Biology & Medicine2020，17，2： | 3 |
| 17 | Proteomic survey towards the tissue-specific proteins of mouse mitochondria显示文摘Mitochondrion plays the key functions in mammalian cells. It is believed that mitochondrion exerts the common biologic functions in many tissues, but also performs some specific functions correspondent with tissues where it is localized. To identify the tissue-specific mitochondrial proteins, we carried out a systematic survey towards mitochondrial proteins in the tissues of C57BL/6J mouse, such as liver, kidney and heart. The mitochondrial proteins were separated by 2DE and identified by MALDI-TOF/TOF MS. Total of 87 unique proteins were identified as the tissue-specific ones, and some representatives were further verified through ICPL quantification and Western blot. Because these issue-specific proteins are coded from nuclear genes, real-time PCR was employed to examine the mRNA status of six typical genes found in the tissues.With combining of the expression data and the co-localization images obtained from confocal microscope, we came to the conclusion that the tissue-specifically mitochondrial proteins were widely distributed among the mouse tissues. Our investigation, therefore, indeed provides a solid base to further explore the biological significance of the mitochondrial proteins with tissue-orientation. | WANG Yuan SUN HaiDan RU YaWei YIN SongYue YIN Liang LIU SiQi | 2011 | Science China(Life Sciences)2011，54，1： | 3 |
| 18 | Alterations of endogenous metabolites in urine of rats exposed to decabromodiphenyl ether using metabonomic approaches显示文摘There is large usage of polybrominated diphenyl ethers(PBDEs) especially for decabromodiphenyl ether(BDE-209, Deca-BDE) in controlling the risks of fire. The toxicological effects of PBDEs are worth being concerned about. Female SD rats were daily gavaged with BDE-209 ether at the dose of 100 mg/kg for 20 days. Histological observation was performed for the screening of the target organs for BDE-209 exposure. The distribution and metabolism of PBDEs in the exposed main organs were evidenced by HRGC-HRMS. Alterations of the endogenous metabolite concentrations in urine were investigated using metabonomic approaches based on1H NMR spectrum. Histopathological changes including serious edema in kidney, hepatocellular spotty necrosis and perivasculitis in liver indicated that BDE-209 caused potential influences on endogenous metabolism in the exposed liver and the kidney. BDE-209 was found to be highly accumulated in lipid, ovary, kidney and liver after 20 days' exposure. Occurrence of other lower brominated PBDEs in the rats demonstrated that reductive debromination process happened in vivo. Hydroxylated and methoxylated–BDEs, as metabolism products, were also detected in the rat tissues. A total of 12 different endogenous metabolites showed obvious alterations in urine from the exposed rats, indicating the disturbance of the corresponding internal biochemical processes induced by BDE-209 exposure. These findings in vivo suggested the potential health risk might be of concern due to the toxicological effects of BDE-209 as a ubiquitous compound in the environment. | Weijin Yang Jianjie Fu Thanh Wang Hanxia Liu Yawei Wang Qunfang Zhou Guibin Jiang | 2014 | Journal of Environmental Sciences2014，26，4： | 3 |
| 19 | Rare-earth based materials:an effective toolbox for brain imaging,therapy,monitoring and neuromodulation显示文摘Brain diseases, including tumors and neurodegenerative disorders, are among the most serious health problems. Non-invasively high-resolution imaging methods are required to gain anatomical structures and information of the brain. In addition, efficient diagnosis technology is also needed to treat brain disease. Rare-earth based materials possess unique optical properties, superior magnetism, and high X-ray absorption abilities, enabling high-resolution imaging of the brain through magnetic resonance imaging, computed tomography imaging, and fluorescence imaging technologies. In addition, rare-earth based materials can be used to detect, treat, and regulate of brain diseases through fine modulation of their structures and functions. Importantly, rare-earth based materials coupled with biomolecules such as antibodies, peptides, and drugs can overcome the blood-brain barrier and be used for targeted treatment. Herein, this review highlights the rational design and application of rare-earth based materials in brain imaging, therapy, monitoring, and neuromodulation. Furthermore, the development prospect of rare-earth based materials is briefly introduced. | Zheng Wei Yawei Liu Bo Li Jingjing Li Shuang Lu Xiwen Xing Kai Liu Fan Wang Hongjie Zhang | 2022 | Light(Science & Applications)2022，11，8： | 3 |
| 20 | Long Noncoding RNA MALAT-1 is a New Potential Therapeutic Target for Castration-Resistant Prostate Cancer显示文摘 | Shancheng Ren Yawei Liu Weidong Xu Yi Sun Ji Lu Fubo Wang Min Wei Jian Shen Jianguo Hou Xu Gao Chuanliang Xu Jiaoti Huang Yi Zhao Yinghao Sun | 2013 | The Journal of Urology2013，，： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员