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| 1 | Exosomes Released from Rabies Virus-Infected Cells May be Involved in the Infection Process显示文摘Exosomes are cell-derived vesicles that are secreted by many eukaryotic cells. It has recently attracted attention as vehicles of intercellular communication. Virus-infected cells release exosomes, which contain viral proteins, RNA, and pathogenic molecules. However, the role of exosomes in virus infection process remains unclear and needs to be further investigated.In this study, we aimed to evaluate the effects of exosomes on rabies virus infection. OptiPrep^(TM) density gradient centrifugation was used to isolate exosomes from rabies virus-infected cell culture supernatants. A rabies virus G protein enzyme-linked immunosorbent assay and acetylcholinesterase activity assays were performed to verify the centrifugation fractions. Exosomes were then characterized using transmission electron microscopy and Western blotting. Our results showed that rabies virus infection increased the release of exosomes. Treatment with GW4869 and si-Rab27 a, two exosomal secretion inhibitors, inhibited exosome release. Furthermore, the inhibitors reduced the levels of extracellular and intracellular viral RNA. These data indicated that exosomes may participate in the viral infection process. Moreover, our results establish a basis for future research into the roles of exosomes in rabies virus infection and as potential targets for developing new antiviral strategies. | Jingyu Wang Fan Wu Chuntian Liu Wenwen Dai Yawei Teng Weiheng Su Wei Kong Feng Gao Linjun Cai Ali Hou Chunlai Jiang | 2019 | Virologica Sinica2019，34，1： | 3 |
| 2 | Assays of urine levels of HNL/NGAL in patients undergoing cardiac surgery and the impact of antibody configuration on their clinical performances显示文摘 | Linjun Cai Jan Borowiec Shengyuan Xu Wenyu Han Per Venge | 2009 | Clinica Chimica Acta2009，，1： | 1 |
| 3 | Characterization and Candidate Gene Analysis of the Yellow-Green Leaf Mutant ygl16 in Rice(Oryza sativa L.)显示文摘Leaf color mutants are ideal materials for studying many plant physiological and metabolic processes such as photosynthesis,photomorphogenesis,hormone physiology and disease resistance.In this study,the genetically stable yellow-green leaf mutant ygl16 was identified from mutated“Xinong 1B”.Compared with the wild type,the pigment concentration and photosynthetic capacity of the ygl16 decreased significantly.The ultrastructural observation showed that the distribution of thylakoid lamellae was irregular in ygl16 chloroplasts,and the grana and matrix lamellae were blurred and loose in varied degrees,and the chloroplast structure was disordered,while the osmiophilic corpuscles increased.The results of the genetic analysis and mapping showed that the phenotype of ygl16 was controlled by a pair of recessive nuclear gene.The gene located in the 56Kb interval between RM25654 and R3 on the long arm of chromosome 10.The sequencing results showed that the 121st base of the first intron of the candidate gene OsPORB/FGL changed from A to T in the interval.qRT-PCR results showed that the expression of chlorophyll synthase-related genes in the mutant decreased. | Linjun Cai Junhua Liu Han Yun Dan Du Xiaolong Zhong Zhenlin Yang Xianchun Sang Changwei Zhang | 2021 | Phyton-International Journal of Experimental Botany2021，90，4： | 1 |
| 4 | Transcriptome-wide profiling of RNA N^(4)-cytidine acetylation in Arabidopsis thaliana and Oryza sativa显示文摘Acetylation of N^(4)-cytidine(ac^(4)C)has recently been discovered as a novel modification of mRNA.RNA ac^(4)C modification has been shown to be a key regulator of RNA stability,RNA translation,and the thermal stress response.However,its existence in eukaryotic mRNAs is still controversial.In plants,the existence,distribution pattern,and potential function of RNA ac^(4)C modification are largely unknown.Here we report the presence of ac^(4)C in the mRNAs of both Arabidopsis thaliana and rice(Oryza sativa).By comparing two ac^(4)C sequencing methods,we found that RNA immunoprecipitation and sequencing(acRIP-seq),but not ac^(4)C sequencing,was suitable for plant RNA ac^(4)C sequencing.We present transcriptome-wide atlases of RNA ac^(4)C modification in A.thaliana and rice mRNAs obtained by acRIP-seq.Analysis of the distribution of RNA ac^(4)C modifications showed that ac^(4)C is enriched near translation start sites in rice mRNAs and near translation start sites and translation end sites in Arabidopsis mRNAs.The RNA ac^(4)C modification level is positively correlated with RNA half-life and the number of splicing variants.Similar to that in mammals,the translation efficiency of ac^(4)C target genes is significantly higher than that of other genes.Our in vitro translation results confirmed that RNA ac^(4)C modification enhances translation efficiency.We also found that RNA ac^(4)C modification is negatively correlated with RNA structure.These results suggest that ac^(4)C is a conserved mRNA modification in plants that contributes to RNA stability,splicing,translation,and secondary structure formation. | Bin Li Donghao Li Linjun Cai Qiting Zhou Cong Liu Jianzhong Lin Yixing Li Xiaoying Zhao Li Li Xuanming Liu Chongsheng He | 2023 | Molecular Plant2023，16，6： | 0 |
| 5 | A Cross-Border E-Commerce Intellectual Property Rights显示文摘As an intangible property right,intellectual property is a very important economic resource,which is of great significance for merchants to enter the international market.With the development of The Times,more and more merchants begin to look globally and enter the overseas market.In order to gain a foothold and develop in the international market where intellectual property rights are more strictly protected,intellectual property rights have important significance that cannot be ignored by all businesses.Writing significance:Taking NetEase Koala as an example to further understand the IPR protection of cross-border e-commerce. | Linjun Cai | 2020 | Journal of Finance Research2020，4，1： | 0 |
| 6 | Functionalization of Magnetic Titanium Dioxide for Targeted Drug Delivery and UV-induced Release显示文摘Magnetic titanium dioxide core-shell nanoparticles(Fe3O4@TiO2 NPs) were synthesized as particles withappropriate size for intravenous injection. They subsequently were functionalized with p-aminothiophenol(PATP) tofacilitate the covalent attachment of target drugs through azo coupling reaction, which can be universally observed ina variety of drugs containing phenols, anilines, and imidazoles. The proposed drug nanocarrier was designed to po-ssess the ability to release drugs upon UV irradiation because of the photocatalytic property of the TiO2 shells. All theresults indicate the potential application of the Fe3O4@TiO2 NPs in targeted drug delivery with greater chemical sta-bility and universality. | CAI Linjun LI Junbo WANG Shipeng ZHAO Mengzhe ZHAO Bing JIANG Chunlai KONG Wei | 2017 | Chemical Research in Chinese Universities2017，33，2： | 0 |
| 7 | RASGRP1 targeted by H3K27me3 regulates myoblast proliferation and differentiation in mice and pigs显示文摘Skeletal muscle is not only the largest organ in the body that is responsible for locomotion and exercise but also crucial for maintaining the body’s energy metabolism and endocrine secretion.The trimethylation of histone H3 lysine 27(H3K27me3)is one of the most important histone modifications that participates in muscle development regulation by repressing the transcription of genes.Previous studies indicate that the RASGRP1 gene is regulated by H3K27me3 in embryonic muscle development in pigs,but its function and regulatory role in myogenesis are still unclear.In this study,we verify the crucial role of H3K27me3 in RASGRP1 regulation.The gain/loss function of RASGRP1 in myogenesis regulation is performed using mouse myoblast C2C12 cells and primarily isolated porcine skeletal muscle satellite cells(PSCs).The results of qPCR,western blot analysis,EdU staining,CCK-8 assay and immunofluorescence staining show that overexpression ofRASGRP1 promotes cell proliferation and differentiation in both skeletal muscle cell models,while knockdown of RASGRP1 leads to the opposite results.These findings indicate that RASGRP1 plays an important regulatory role in myogenesis in both mice and pigs. | Liyao Xiao Jiaxin Qiao Yiyang Huang Baohua Tan Linjun Hong Zicong Li Gengyuan Cai Zhenfang Wu Enqin Zheng Shanshan Wang Ting Gu | 2024 | Acta Biochimica et Biophysica Sinica2024，56，3： | 0 |
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