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| 1 | A review on phospholipids and their main applications in drug delivery systems显示文摘Phospholipids have the characteristics of excellent biocompatibility and a especial amphiphilicity.These unique properties make phospholipids most appropriate to be employed as important pharmaceutical excipients and they have a very wide range of applications in drug delivery systems.The aim of this review is to summarize phospholipids and some of their related applications in drug delivery systems,and highlight the relationship between the properties and applications,and the effect of the species of phospholipids on the efficiency of drug delivery.We refer to some relevant literatures,starting from the structures,main sources and properties of phospholipids to introduce their applications in drug delivery systems.The present article focuses on introducing five types of carriers based on phospholipids,including liposomes,intravenous lipid emulsions,micelles,drug-phospholipids complexes and cochleates. | Jing Li Xuling Wang Ting Zhang Chunling Wang Zhenjun Huang Xiang Luo Yihui Deng | 2015 | Asian Journal of Pharmaceutical Sciences2015，10，2： | 23 |
| 2 | Identification of an endogenous glutamatergic transmitter system controlling excitability and conductivity of atrial cardiomyocytes显示文摘As an excitatory transmitter system,the glutamatergic transmitter system controls excitability and conductivity of neurons.Since both cardiomyocytes and neurons are excitable cells,we hypothesized that cardiomyocytes may also be regulated by a similar system. | Duanyang Xie Ke Xiong Xuling Su Guanghua Wang Qiang Ji Qicheng Zou Lingling Wang Yi Liu Dandan Liang Jinfeng Xue Luxin Wang Xueting Gao Xingdong Gu Hongyu Liu r Xiaoyu He Li Li Jian Yang Youming Lu Luying Peng Yi-Han Chen | 2021 | Cell Research2021，31，9： | 5 |
| 3 | A New Indicator for Global Food Security Assessment: Harvested Area Rather Than Cropland Area显示文摘Cropland area has long been used as a key indicator of food security.However,grain yield is not solely controlled by the area of the cropland.Therefore,we proposed a new indicator to assess food security.Results show that from 1992 to 2004,the global cropland area increased by 840200 km^(2)(99.4%),but the grain yield increased only by 310 million t(29.1%);and from 2004 to 2015,the cropland area decreased by 39000 km^(2)(4.64%),but the grain yield increased by 370 million t(70.84%).This result showed that grain yield was not linearly correlated with cropland area,and delimiting the threshold of cropland protection may not guarantee food security.Combined with further correlation analysis,we found that the increase in the global grain yield was more closely related to the harvested area(R^(2)=0.94),which indicated that the harvested area is a more scientific and accurate indicator than cropland area in terms of guaranteeing food security.Therefore,if governments want to ensure the food security,they should choose a new and more accurate indicator:harvested area rather than cropland area. | SONG Fengjiao WANG Shijie BAI Xiaoyong WU Luhua WANG Jinfeng LI Chaojun CHEN Huan LUO Xuling XI Huipeng ZHANG Sirui LUO Guofeng YAN Mengqi ZHEN Qingqing | 2022 | Chinese Geographical Science2022，32，2： | 2 |
| 4 | The anticancer efficacy of pixantrone-loaded liposomes decorated with sialic acid-octadecylamine conjugate显示文摘 | Zhennan She Ting Zhang Xuling Wang Xuan Li Yanzhi Song Xiaobo Cheng Zhenjun Huang Yihui Deng | 2014 | Biomaterials2014，，： | 2 |
| 5 | Research and development of a novel subunit vaccine for the currently circulating pseudorabies virus variant in China显示文摘Pseudorabies(PR)is a devastating viral disease which leads to fatal encephalitis and respiratory disorders in pigs.Commercial gE-deleted live pseudorabies virus(PRV)vaccine has been widely used to control this disease in China.However,the new-emerging variants of PRV compromises the protection provided by current vaccines and lead to the outbreak of PR in vaccinated pig herds.Several killed and live vaccine candidates based on current PRV variants have been reported to be effective to control the disease.A subunit vaccine based on gB protein,one major PRV glycoprotein which elicits strong humoral and cellular immune responses,however,was never evaluated for protection against the current circulating PRV variants.In this study,full-length PRV gB protein was successfully expressed in baculovirus/insect cells in the soluble format and was tested on 3-week-old piglets as a subunit vaccine.Compared with unvaccinated pigs,the gB-vaccinated pigs developed specific antibody-mediated responses and were protected from the virulent PRV HN1201 challenge.All vaccinated pigs survived without showing any PRV-specific respiratory and neurological signs,but all unvaccinated pigs died within 7 days after HN1201 challenge.Hence,this novel gB-based vaccine could be applied as an effective subunit vaccine to control PRV variant in China. | Yuzhou WANG Tongyan WANG He YAN Fanli YANG Linghua GUO Qingyuan YANG Xule HU Feifei TAN Yan XIAO Xiangdong LI Kegong TIAN | 2015 | Frontiers of Agricultural Science and Engineering2015，2，3： | 2 |
| 6 | A simple inorganic-solvent-thermal route to nanocrystalline niobium diboride显示文摘 | Jianhua Ma Yihong Du Meining Wu Guoxing Li Zhiqing Feng Ming Guo Yixiu Sun Weihua Song Mingjin Lin Xule Guo | 2008 | Journal of Alloys and Compounds2008，，： | 1 |
| 7 | Sinoatrial node pacemaker cells share dominant biological properties with glutamatergic neurons显示文摘Activation of the heart normally begins in the sinoatrial node(SAN).Electrical impulses spontaneously released by SAN pacemaker cells(SANPCs)trigger the contraction of the heart.However,the cellular nature of SANPCs remains controversial.Here,we report that SANPCs exhibit glutamatergic neuron-like properties.By comparing the single-cell transcriptome of SANPCs with that of cells from primary visual cortex in mouse,we found that SANPCs co-clustered with cortical neurons.Tissue and cellular imaging confirmed that SANPCs contained key elements of glutamatergic neurotransmitter system,expressing genes encoding glutamate synthesis pathway(G/s),ionotropic and metabotropic glutamate receptors(Grina,Gria3,Grm1 and Grm5)t and glutamate transporters(Slc17a7).SANPCs highly expressed cell markers of glutamatergic neurons(Snap25 and S/-c17a7)t whereas Gad1,a marker of GABAergic neurons,was negative.Functional studies revealed that inhibition of glutamate receptors or transporters reduced spontaneous pacing frequency of isolated SAN tissues and spontaneous Ca2+transients frequency in single SANPC.Collectively,our work suggests that SANPCs share dominant biological properties with glutamatergic neurons,and the glutamatergic neurotransmitter system may act as an intrinsic regulation module of heart rhythm,which provides a potential intervention target for pacemaker cell-associated arrhythmias. | Dandan Liang Zhigang Xue Jinfeng Xue Duanyang Xie Ke Xiong Huixing Zhou Fulei Zhang Xuling Su Guanghua Wang Qicheng Zou Yi Liu Jian Yang Honghui Ma Luying Peng Chunyu Zeng Gang Li Li Wang Yi-Han Chen | 2021 | Protein & Cell2021，12，7： | 1 |
| 8 | A new method to de- termine the projected coordinate origin of cone - beam CT system using elliptical projection显示文摘 | Yang Min Jin Xuling Li Baolei | 2010 | Chinese Physics C (Eng)2010，34，10： | 1 |
| 9 | Analysis of the Immune Response by Standardized Whole‑Blood Stimulation with Metabolism Modulation显示文摘The immune system defends the body from infection and plays a vital role in a wide range of health conditions.Metabolism afects a series of physiological processes,including those linked to the function of human immune system.Cellular metabolism modulates immune cell activation and cytokine production.Understanding the relationship between metabolism and immune response has important implications for the development of immune-based therapeutics.However,the deployment of large-scale functional assays to investigate the metabolic regulation of immune response has been limited by the lack of standardized procedures.Here,we present a protocol for the analysis of immune response using standardized whole-blood stimulation with metabolism modulation.Diverse immune stimuli including pattern recognition receptor(PRR)ligands and microbial stimuli were incubated with fresh human whole blood.The metabolic inhibitors were used to modulate metabolic status in the immune cells.The variable immune responses after metabolic interventions were evaluated.We described in detail the main steps involved in the whole-blood stimulation and cytokines quantifcation,namely,collection and treatment of whole blood,preparation of samples and controls,cytokines detection,and stimulation with metabolic interventions.The metabolic inhibitors for anabolic pathways and catabolic pathways exert selective efects on the production of cytokines from immune cells.In addition to a robust and accurate assessment of immune response in cohort studies,the standardized whole-blood stimulation with metabolic regulation might provide new insights for modulating immunity. | Jialin Zhao Xuling Han Helian Li Yali Luo Yan Fang Yun Wang Jian Gao Yiran Zhao Jingxuan Han Feng Qian | 2024 | Phenomics2024，4，1： | 0 |
| 10 | simple and effective aerosol pathogen disinfection test for a flowing air disinfector显示文摘Aerosol transmission is an important disease transmission route and has been especially pertinent to hospital and biosafety laboratories during the SARS-CoV-2 pandemic.The thermal resistance of airborne SARS-CoV-2 is lower than that of Bacillus subtilis spores,which are often used to test the effectiveness of SARS-CoV-2 and other pathogen disinfection methods.Herein,we propose a new method to test the disinfection ability of a flowing air disinfector(a digital electromagnetic induction air heater)using B.subtilis spores.The study provides an alternative air disinfection test method.The new test system combined an aerosol generator and a respiratory filter designed in-house and could effectively recover spores on the filter membrane at the air outlet after passing through the flowing air disinfector.The total number of bacterial spores used in the test was within the range of 5×10^(5)–5×10^(6)colony-forming units(CFUs)specified in the technical standard for disinfection.The calculation was based on the calculation method in Air Disinfection Effect Appraisal Test in Technical Standard for Disinfection(2002 Edition).At an air speed of 3.5 m/s,we used a digital electromagnetic induction air heater to disinfect flowing air containing 4.100×10^(6)CFUs of B.subtilis spores and determined that the minimum disinfection temperature was 350℃for a killing rate of 99.99%.At 400℃,additional experiments using higher spore concentrations(4.700×10^(6)±1.871×10^(5)CFU)and a higher airspeed(4 m/s)showed that the killing rate remained>99.99%.B.subtilis spores,as a biological indicator for testing the efficiency of dry-heat sterilization,were killed by the high temperatures used in this system.The proposed method used to test the flowing air disinfector is simple,stable,and effective.This study provides a reference for the development of test systems that can assess the disinfection ability of flowing air disinfectors. | Xuling Liu Zhiran Qin Linqing Wang Xiaoting Xie Yifang Fu Jianhai Yu Zuxin Liang Xiaoen He Jingshu Li Hong Dai Jinxiu Yao Qinghua Wu Weiwei Xiao Li Zhu Chengsong Wan Bao Zhang Wei Zhao | 2023 | Journal of Biosafety and Biosecurity2023，5，1： | 0 |
| 11 | Deep Immunophenotyping of Human Whole Blood by Standardized Multi‑parametric Flow Cytometry Analyses显示文摘Immunophenotyping is proving crucial to understanding the role of the immune system in health and disease.High-through-put flow cytometry has been used extensively to reveal changes in immune cell composition and function at the single-cell level.Here,we describe six optimized 11-color flow cytometry panels for deep immunophenotyping of human whole blood.A total of 51 surface antibodies,which are readily available and validated,were selected to identify the key immune cell populations and evaluate their functional state in a single assay.The gating strategies for effective flow cytometry data analysis are included in the protocol.To ensure data reproducibility,we provide detailed procedures in three parts,including(1)instrument characterization and detector gain optimization,(2)antibody titration and sample staining,and(3)data acquisition and quality checks.This standardized approach has been applied to a variety of donors for a better understanding of the complexity of the human immune system. | Jian Gao Yali Luo Helian Li Yiran Zhao Jialin Zhao Xuling Han Jingxuan Han Huiqin Lin Feng Qian | 2023 | Phenomics2023，3，3： | 0 |
| 12 | Acetyl-L-carnitine protects adipose-derived stem cells exposed to H2O2 through regulating AMBRA1-related autophagy显示文摘The cell activity of adipose-derived stem cells(ADSCs)is affected by the intracellular reactive oxygen species(ROS)and the level of autophagy.Previous studies reveal that acetyl-L-carnitine(ALC)possesses capacities of resisting oxidative stress and regulating autophagy.Activating molecule in Beclin1-regulated autophagy protein 1(AMBRA1)plays a key role in initiating Beclin1-regulated autophagy.In the present study,we discovered ALC pretreatment(1 mM,24 h)significantly increased the activity of ADSCs exposed to H_(2)O_(2)(100μM,2 h)in vitro with improved stemness,and reduced the production of intracellular ROS.In addition,we found for the first time that ALC treatment up-regulated autophagy of ADSCs through strengthening the expressions of Beclin1 and AMBRA1 synchronously,which might be involved in the protective effect of ALC. | YAO QIAN JINGPING WANG ZIWAN JI HAO CHEN YUCANG HE XULING LV ZIKAI ZHANG TIAN LI TIANYUN PAN LIQUN LI MING LIN | 2021 | BIOCELL2021，45，1： | 0 |
| 13 | Long noncoding RNA lnc_217 regulates hepatic lipid metabolism by modulating lipogenesis and fatty acid oxidation显示文摘Nonalcoholic fatty liver disease (NAFLD) is considered a major health epidemic with an estimated 32.4% worldwide prevalence. No drugs have yet been approved and therapeutic nodes remain a major unmet need. Long noncoding RNAs are emerging as an important class of novel regulators influencing multiple biological processes and the pathogenesis of NAFLD. Herein, we described a novel long noncoding RNA, lnc_217, which was liver enriched and upregulated in high-fat diet-fed mice, and a genetic animal model of NAFLD. We found that liver specific knockdown of lnc_217 was resistant to high-fat diet-induced hepatic lipid accumulation and decreased serum lipid in mice. Mechanistically, we demonstrated that knockdown of lnc_217 not only decreased de novo lipogenesis by inhibiting sterol regulatory element binding protein-1c cleavage but also increased fatty acid β- oxidation through activation of peroxisome proliferator-activated receptor α and carnitine palmitoyltransferase-1α. Taken together, we conclude that lnc_217 may be a novel regulator of hepatic lipid metabolism and a potential therapeutic target for the treatment of hepatic steatosis and NAFLD-related metabolic disorders. | Xiaoqing Yuan Yawei Liu Xule Yang Yun Huang Xuan Shen Hui Liang Hongwen Zhou Qian Wang Xu Zhang John Zhong Li | 2023 | The Journal of Biomedical Research2023，37，6： | 0 |
| 14 | Mixed PEGylated surfactant modifying system decrease the accelerated blood clearance phenomenon of nanoemulsions in rats显示文摘The accelerated blood clearance(ABC) phenomenon which is induced by repeated injection of poly(ethylene glycol)(PEG)-coated colloidal carriers gives clinical challenge to the promising drug delivery system. It is necessary to decrease this unexpected immunological response.A novel 4-arm poly(ethylene glycol-5000)4-cholesteryl methyl amide(4-arm PEG5000-CHMA)has been synthesized. The structure of 4-arm PEG5000-CHMA was confirmed by IR and 1H-NMR spectrum. The pharmacokinetics of the tocopheryl nicotinate(TN)-loaded nanoemulsions modified with 4-arm PEG5000-CHMA or/and 1, 2-distearoyl-Sn-glycero-3-phosphoethanolamine-n-[methoxy(poly-ethyleneglycol)-2000](mPEG2000-DSPE) have been studied. Furthermore, the ABC phenomenon has been detailed investigated in rats by TN-loaded nanoemulsions modified with 4-arm PEG5000-CHMA and mPEG2000-DSPE(CPNE). The plasma levels of TN and anti-PEG IgM antibody were determined by HPLC and ELISA, respectively.The circulation time of the CPNEs were comparable to the mPEG2000-DSPE coated nanoemulsions. Moreover, the ABC phenomenon can be decreased by CPNEs. This study designs a method to decrease the ABC phenomenon and develops a clinical promising nanoemulsion for therapeutic or imaging purpose. | Yuqing Su Wenya Tang Yanzhi Song Chunling Wang Qingjing Tian Xuling Wang Jingjing Quan Buoqun Li Shaoning Wang Yihui Deng | 2017 | Asian Journal of Pharmaceutical Sciences2017，12，1： | 0 |
| 15 | A CBL4-CIPK6 module confers salt tolerance in cucumber显示文摘Soil salinization is a major threat to cucumbers grown under protected cultivation.Under stressful environments,calcineurin B-like proteins(CBLs)can sense and bind Ca2+signals and regulate CBL-interacting protein kinases(CIPKs)to transmit signals and induce cellular responses.Although CBL-CIPK modules play central roles in plant development and response to various abiotic stresses in Arabidopsis,little is known about their functions in cucumber.In this study,we demonstrate that CsCBL4 interacts with CsCIPK6,which exhibited similar responses to salt stress in cucumber.Furthermore,salt stress resulted in greater accumulation of CsCBL4 and CsCIPK6.Comprehensive phenotype analysis demonstrated that silencing CsCBL4 or CsCIPK6 reduced the salt tolerance of cucumber,and overexpression of CsCBL4 increased the salt tolerance of Arabidopsis.Collectively,these results indicate that the CsCBL4-CsCIPK6 module plays an important role in the resistance of cucumber to salt stress.The information provides insights for the genetic breeding of salt tolerance in cucumber in the future. | Mingfei Wang Songlin Yang Lei Sun Zhongxuan Feng Yiming Gao Xuling Zhai Yuming Dong Haoying Wu Yuanyuan Cui Sen Li Shuo Xu Ezra S.Bartholomew Huazhong Ren Xingwang Liu | 2022 | Vegetable Research2022，2，1： | 0 |
| 16 | AtMYBS1 negatively regulates heat tolerance by directly repressing the expression of MAX1 required for strigolactone biosynthesis in Arabidopsis显示文摘Heat stress caused by global warming requires the development of thermotolerant crops to sustain yield.It is necessary to understand the molecular mechanisms that underlie heat tolerance in plants.Strigolactones(SLs)are a class of carotenoid-derived phytohormones that regulate plant development and responses to abiotic or biotic stresses.Although SL biosynthesis and signaling processes are well established,genes that directly regulate SL biosynthesis have rarely been reported.Here,we report that the MYB-like transcription factor AtMYBS1/AtMYBL,whose gene expression is repressed by heat stress,functions as a negative regulator of heat tolerance by directly inhibiting SL biosynthesis in Arabidopsis.Overexpression of AtMYBS1 led to heat hypersensitivity,whereas atmybs1 mutants displayed increased heat tolerance.Expression of MAX1,a critical enzyme in SL biosynthesis,was induced by heat stress and downregulated in AtMYBS1-overexpression(OE)plants but upregulated in atmybs1 mutants.Overexpression of MAX1 in the AtMYBS1-OE background reversed the heat hypersensitivity of AtMYBS1-OE plants.Loss of MAX1 function in the atmyb1 background reversed the heat-tolerant phenotypes of atmyb1 mutants.Yeast one-hybrid assays,chromatin immunoprecipitation‒qPCR,and transgenic analyses demonstrated that AtMYBS1 directly represses MAX1 expression through the MYB binding site in the MAX1 promoter in vivo.The atmybs1d14 double mutant,like d14 mutants,exhibited hypersensitivity to heat stress,indicating the necessary role of SL signaling in AtMYBS1-regulated heat tolerance.Our findings provide new insights into the regulatory network of SL biosynthesis,facilitating the breeding of heat-tolerant crops to improve crop production in a warming world. | Xiang Li Jianhua Lu Xuling Zhu Yanqi Dong Yanli Liu Shanshan Chu Erhui Xiong Xu Zheng Yongqing Jiao | 2023 | Plant Communications2023，4，6： | 0 |
| 17 | Analysis of the Implementation of Integrated Healthcare Management in Preoperative Preparation显示文摘Objective:To strengthen preoperative preparation management and reduce preoperative preparation defects.Methods:We set up a special medical care management team to investigate the preoperative preparation in surgical departments,analyze the causes of the shortcomings in preoperative preparation,and propose countermeasures.The medical staff jointly formulated plans related to preoperative preparation management including clear outline of responsibilities,strengthening of training,integrated medical care,timely feedback,and continuous improvement.Besides,the preoperative preparation of patients before and after the improvement measures were compared using statistical analysis.Results:The rate of inadequate preoperative preparation was 11.14 per thousand before the integrated management model was implemented.After the implementation of the integrated management model,the rate of inadequate preoperative preparation decreased to 2.99 per thousand,and the difference was statistically(P<0.05).Conclusion:The integrated management of preoperative preparation can help reduce the rate of preoperative complications and ensure the safety of surgery. | Huiling Liu Xuling Li Xinyi Liu Yuhua Ma Haomin Li Cuiqing Liu | 2023 | Journal of Clinical and Nursing Research2023，7，3： | 0 |
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