2篇
您的检索式：作者名="Xuling Zhu"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | simple and effective aerosol pathogen disinfection test for a flowing air disinfector显示文摘Aerosol transmission is an important disease transmission route and has been especially pertinent to hospital and biosafety laboratories during the SARS-CoV-2 pandemic.The thermal resistance of airborne SARS-CoV-2 is lower than that of Bacillus subtilis spores,which are often used to test the effectiveness of SARS-CoV-2 and other pathogen disinfection methods.Herein,we propose a new method to test the disinfection ability of a flowing air disinfector(a digital electromagnetic induction air heater)using B.subtilis spores.The study provides an alternative air disinfection test method.The new test system combined an aerosol generator and a respiratory filter designed in-house and could effectively recover spores on the filter membrane at the air outlet after passing through the flowing air disinfector.The total number of bacterial spores used in the test was within the range of 5×10^(5)–5×10^(6)colony-forming units(CFUs)specified in the technical standard for disinfection.The calculation was based on the calculation method in Air Disinfection Effect Appraisal Test in Technical Standard for Disinfection(2002 Edition).At an air speed of 3.5 m/s,we used a digital electromagnetic induction air heater to disinfect flowing air containing 4.100×10^(6)CFUs of B.subtilis spores and determined that the minimum disinfection temperature was 350℃for a killing rate of 99.99%.At 400℃,additional experiments using higher spore concentrations(4.700×10^(6)±1.871×10^(5)CFU)and a higher airspeed(4 m/s)showed that the killing rate remained>99.99%.B.subtilis spores,as a biological indicator for testing the efficiency of dry-heat sterilization,were killed by the high temperatures used in this system.The proposed method used to test the flowing air disinfector is simple,stable,and effective.This study provides a reference for the development of test systems that can assess the disinfection ability of flowing air disinfectors. | Xuling Liu Zhiran Qin Linqing Wang Xiaoting Xie Yifang Fu Jianhai Yu Zuxin Liang Xiaoen He Jingshu Li Hong Dai Jinxiu Yao Qinghua Wu Weiwei Xiao Li Zhu Chengsong Wan Bao Zhang Wei Zhao | 2023 | Journal of Biosafety and Biosecurity2023，5，1： | 0 |
| 2 | AtMYBS1 negatively regulates heat tolerance by directly repressing the expression of MAX1 required for strigolactone biosynthesis in Arabidopsis显示文摘Heat stress caused by global warming requires the development of thermotolerant crops to sustain yield.It is necessary to understand the molecular mechanisms that underlie heat tolerance in plants.Strigolactones(SLs)are a class of carotenoid-derived phytohormones that regulate plant development and responses to abiotic or biotic stresses.Although SL biosynthesis and signaling processes are well established,genes that directly regulate SL biosynthesis have rarely been reported.Here,we report that the MYB-like transcription factor AtMYBS1/AtMYBL,whose gene expression is repressed by heat stress,functions as a negative regulator of heat tolerance by directly inhibiting SL biosynthesis in Arabidopsis.Overexpression of AtMYBS1 led to heat hypersensitivity,whereas atmybs1 mutants displayed increased heat tolerance.Expression of MAX1,a critical enzyme in SL biosynthesis,was induced by heat stress and downregulated in AtMYBS1-overexpression(OE)plants but upregulated in atmybs1 mutants.Overexpression of MAX1 in the AtMYBS1-OE background reversed the heat hypersensitivity of AtMYBS1-OE plants.Loss of MAX1 function in the atmyb1 background reversed the heat-tolerant phenotypes of atmyb1 mutants.Yeast one-hybrid assays,chromatin immunoprecipitation‒qPCR,and transgenic analyses demonstrated that AtMYBS1 directly represses MAX1 expression through the MYB binding site in the MAX1 promoter in vivo.The atmybs1d14 double mutant,like d14 mutants,exhibited hypersensitivity to heat stress,indicating the necessary role of SL signaling in AtMYBS1-regulated heat tolerance.Our findings provide new insights into the regulatory network of SL biosynthesis,facilitating the breeding of heat-tolerant crops to improve crop production in a warming world. | Xiang Li Jianhua Lu Xuling Zhu Yanqi Dong Yanli Liu Shanshan Chu Erhui Xiong Xu Zheng Yongqing Jiao | 2023 | Plant Communications2023，4，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员