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| 1 | H pylori (CagA) and Epstein-Barr virus infection in gastric carcinomas:Correlation with p53 mutation and c-Myc,Bcl-2 and Bax expression显示文摘AIM: To investigate the interrelationship between H pylori and Epstein-Barr virus (EBV) infection in the gastric carcinogenesis having in focus the p53 mutation and the c-Myc, Bcl-2 and Bax expression. METHODS: seventy-one gastric carcinoma tissues were assessed by polymerase chain reaction (PCR) for H pylori and in situ hybridization for EBV. c-Myc, Bcl-2 and Bax expression were detected by immunohistochemistry and single-stranded conformational polymorphism (SSCP) for p53 mutation. RESULTS: The positivity rates for H pylori and EBV were 94.4% and 8.45%, respectively. The majority of the cases displayed only the H pylori presence. All EBV positive cases were also H pylori positive. None infectious agent was observed in 5.55% of the cases. The intestinal type tumor was more frequent in the co-infected and non-infected groups. The female predominated in the non-infected group showing statistical significance (70.4% vs 29.6%, P=0.039). The Bcl-2 was only detected in the group exclusively infected by H pylori. However, c-Myc and Bax were detected in the three groups but with a low frequency in the co-infected group. Mutation of p53 was present in all groups, with the highest frequencies in the H pylori positive groups. CONCLUSION: The frequency of H pylori infection in gastric carcinomas was high. The presented data indicated that gastric carcinogenesis has different pathways depending of the presence of the two investigated infectious agents, suggesting a possible involvement of H pylori with apoptotic process. The low expression of c-Myc and Bax in the EBV-positive groups suggests that EBV may inhibit the expression of these proteins. Nevertheless, p53 mutation shows to be a relevant alteration, independent of both infectious agents. | Valeska Portela Lima Marcos Antonio Pereira de Lima Angela Rosa André Márcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Sílvia Helena Barem Rabenhorst | 2008 | World Journal of Gastroenterology2008，14，6： | 17 |
| 2 | Phase angle obtained by bioelectrical impedance analysis independently predicts mortality in patients with cirrhosis显示文摘AIM To evaluate the prognostic value of the phase angle(PA)obtained from bioelectrical impedance analysis(BIA) for mortality prediction in patients with cirrhosis. METHODS In total, 134 male cirrhotic patients prospectively completed clinical evaluations and nutritional assessment by BIA to obtain PAs during a 36-mo follow-up period. Mortality risk was analyzed by applying the PA cutoff point recently proposed as a malnutrition marker(PA ≤ 4.9°) in Kaplan-Meier curves and multivariate Cox regression models. RESULTS The patients were divided into two groups according to the PA cutoff value(PA > 4.9°, n = 73; PA ≤ 4.9°, n = 61). Weight, height, and body mass index were similar in both groups, but patients with PAs > 4.9° were younger and had higher mid-arm muscle circumference, albumin, and handgrip-strength values and lower severe ascites and encephalopathy incidences, interleukin(IL)-6/IL-10 ratios and C-reactive protein levels than did patients with PAs ≤ 4.9°(P ≤ 0.05). Forty-eight(35.80%) patients died due to cirrhosis, with a median of 18 mo(interquartile range, 3.3-25.6 mo) follow-up until death. Thirty-one(64.60%) of these patients were from the PA ≤ 4.9° group. PA ≤ 4.9° significantly and independently affected the mortality model adjusted for Model for End-Stage Liver Disease score and age(hazard ratio = 2.05, 95%CI: 1.11-3.77, P = 0.021). In addition, Kaplan-Meier curves showed that patients with PAs ≤ 4.9° were significantly more likely to die. CONCLUSION In male patients with cirrhosis, the PA ≤ 4.9° cutoff was associated independently with mortality and identified patients with worse metabolic, nutritional, and disease progression profiles. The PA may be a useful and reliable bedside tool to evaluate prognosis in cirrhosis. | Giliane Belarmino Maria Cristina Gonzalez Raquel S Torrinhas Priscila Sala Wellington Andraus Luiz Augusto Carneiro D'Albuquerque Rosa Maria R Pereira Valéria F Caparbo Graziela R Ravacci Lucas Damiani Steven B Heymsfield Dan L Waitzberg | 2017 | World Journal of Hepatology2017，9，7： | 11 |
| 3 | Nat Biotechnol:将人星形胶质细胞重编程为多巴胺能神经元,有助治疗帕金森病显示文摘帕金森病是一种主要影响运动系统的神经退行性疾病。它的特征在于大脑中的多巴胺能神经元(dopaminergic neuron)渐进性丧失。尽管当前的疗法旨在补充多巴胺水平,但是没有一种疗法能够恢复这些丢失的细胞。如今。在一项新的研究中,来自瑞典、奥地利、西班牙和美国的研究人员开发出一种方法:将神经胶质细胞(glialcell)转化为活性的多巴胺能神经元,并且所产生的多巴胺能神经元能够部分恢复帕金森病模式小鼠的运动功能。这项概念验证研究可能为开发出一种治疗这种疾病的新方法铺平道路。 | Pia Rivetti di Val Cervo, Elisa Martín-Montañez, Enrique M Toledo, Gioele La Manno, Sara Padrell Sánchez, Sten Linnarsson Ernest Arenas Roman A Romanov, Christian Pifl Tibor Harkany Roman A Romanov, Giada Spigolon, Débora Masini, Michael Feyder, Tibor Harkany Gilberto Fisone Elisa Martín-Montañez Yi-Han Ng Marius Wernig | 2017 | 现代生物医学进展2017，17，17： | 11 |
| 4 | 土壤中的生物多样性与生态系统功能显示文摘我们回顾了目前关于土壤生物多样性及其在生态系统过程中的作用、对于人类目标的重要性及对胁迫和扰动的恢复能力等的研究进展。现存物种的数量远远大于人们已经描述的数量,即使是对于肉眼可见的分类群也是如此。而且也普遍缺乏在生物地理学上的综合描述。迫切需要在分类学和对新一代系统科学家培训上做更大努力。尤其是应当集中致力于就我们所知。对生态系统功能起关键作用的土壤生物类群方面。为了确定这种类群,需要对土壤生物区系的影响圈(sphere of Influence,SOI),如根的生物区系、有机物质破碎者(shredder)和土壤生物扰动者(bioturbator)加以识别,这些影响圈比如可能通过与植物的相互作用而控制着生态系统的过程。在这些SOI中,我们分析出了土壤有机体的功能类群。最迫切需要研究的问题是将物种按功能类群进行区分,并确定功能类群中的物种冗余。这种迫切性是根据为阐述与集约化农业、森林扰动、环境污染和全球环境变化有关的土壤功能丧失程度的需要而确定的。目前认为最处于危险的土壤生物区系是那些大型动物群的有机物破碎者、土壤生物扰动者、某些专门的细菌(如硝化细菌和固氮菌)以及形成真菌的菌根中贫乏物种的功能类群。在开展这些优先性研究时,需要使用长期的和大尺度的田间试验,以及地理统计学和地理信息系统等现代方法进行试验研究。 | Lijbert Brussaard Val M.Behan-Pelletier David E.Bignell Valerie K.Brown Wim Didden Patrica Folgarait Carlos Fragoso Diana Wall Freckman Vadakattu V.S.R.Gupta Tsutomu Hattori's David L.Hawkworth Carole Klopatek Patric Lavelle David W.Malloch Josef Rusek Bengt Sderstrom James M.Tiedje Ross A.Virginia 闵庆文 | 1997 | 人类环境杂志1997，26，8： | 6 |
| 5 | Helicobacter pylori and EBV in gastric carcinomas:Methylation status and microsatellite instability显示文摘AIM:To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA+ and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H. pylori and genotyping were performed by PCR, using specifi c primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the χ2 or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA+ in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H. pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. | Adriana Camargo Ferrasi Nídia Alice Pinheiro Silvia Helena Barem Rabenhorst Otávia Luisa Caballero Maria Aparecida Marchesan Rodrigues Fabrício de Carvalho Celso Vieira de Souza Leite Marcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Maria Inês de Moura Campos Pardini | 2010 | World Journal of Gastroenterology2010，16，3： | 6 |
| 6 | Diffuse intestinal ganglioneuromatosis an uncommon manifestation of Cowden syndrome显示文摘Diffuse intestinal ganglioneuromatosis is a hamartomatous polyposis characterized by a disseminated, intramural or transmural proliferation of neural elements involving the enteric plexuses. It has been associated with MEN Ⅱ, neurofibromatosis type 1 and hamartomatous polyposis associated with phosphatase and tensin homolog mutation. We report the case of a female patient with a history of a breast and endometrial tumor who presented in a colonoscopy performed for rectal bleeding diffuse ganglioneuromatosis, which oriented the search for other characteristic findings of Cowden syndrome given the personal history of the patient. The presence of an esophagogastric polyposis was also noted. Cowden syndrome is characterized by skin lesions, but it is rarely diagnosed by these lesions, because they are usually overlooked. Intestinal polyposis is not a major diagnostic criterion but it is very useful for early diagnosis. The combination of colonic polyposis and glucogenic acanthosis should orient the diagnosis to Cowden syndrome. | Maria Teresa Herranz Bachiller Jesus Barrio Andrés Fernando Pons Noelia Alcaide Suárez Rafael Ruiz-Zorrilla Lorena Sancho del Val Sara Lorenzo Pelayo Carlos De La Serna Higuera Ramon Atienza Sánchez Manuel Perez Miranda | 2013 | World Journal of Gastrointestinal Oncology2013，5，2： | 5 |
| 7 | Intraocular complications of IFN-α and ribavirin therapy in patients with chronic viral hepatitis C显示文摘We report a panel of severe inflammatory and vascular intraocular disorders occurring during interferon-alpha (IFN-α) treatment in eight hepatitis C virus (HCV)-infected patients. These events include three cases of Vogt-Koyanagi-Harada like (VKH) disease (an association of panuveitis, retinal detachment, ear and meningeal detachment and skin and hair changes), two cases of central retinal vein occlusion, one case of central retinal artery occlusion, one case of severe hypertensive retinopathy and one case of bilateral ischemic optic neuropathy with severe visual impairment. Rare as they are, such severe ophthalmological complications require a close follow-up of HCV-infected patients under IFN-α treatment with ophthalmological monitoring if any ocular manifestation occurs. | Damien Sène Valérie Touitou Bahram Bodaghi David Saadoun Gabriel Perlemuter Nathalie Cassoux Jean-Charles Piette Phuc Le Hoang Patrice Cacoub | 2007 | World Journal of Gastroenterology2007，13，22： | 5 |
| 8 | Safety and Efficacy of Antigen-Specific Regulatory T-Cell Therapy for Patients With Refractory Crohn’s Disease显示文摘 | Pierre Desreumaux Arnaud Foussat Matthieu Allez Laurent Beaugerie Xavier Hébuterne Yoram Bouhnik Maria Nachury Valérie Brun Hervé Bastian Nathalie Belmonte Michel Ticchioni Agnès Duchange Patricia Morel–Mandrino Virginie Neveu Nathalie Clerget–Chossat Mig | 2012 | Gastroenterology2012，，5： | 4 |
| 9 | Crohn's disease environmental factors in the developing world: A case-control study in a statewide catchment area in Brazil显示文摘AIM To identify environmental risk factors associated with the development of Crohn's disease(CD) in order to reassess the hygiene hypothesis.METHODS A hospital-based, case-control study was carried out with CD patients(n = 145) and controls(n = 163)representing a socioeconomically diverse statewide catchment area in Brazil. Controls were recruited from caregivers of patients seen in different outpatient clinics at the same hospital. A multi-item survey with 94 questions regarding family history of CD, perinatal and childhood circumstances, living conditions, tobacco use and familial socioeconomic status was carried out by interviewers. RESULTS On the univariate analysis, predictive variables for CD included being male, under age of 40, a high education level, urban dweller, smaller family size, exposure to enteric pathogens and user of treated water(P < 0.005). On the multivariate analysis, variables significantly associated with CD were male gender(OR = 2.09), under age 40(OR = 3.10), white(OR = 2.32), from a small family in childhood(OR = 2.34) and adulthood(OR = 3.02), absence of viral infections in childhood(OR = 2.23), exposure to enteric pathogens(OR = 2.41), having had an appendectomy(OR = 2.47) and prior or current smoker(OR = 2.83/1.12). CONCLUSION Most variables supporting the 'hygiene hypothesis' are associated with the development of CD but are not independent predictors of the diagnosis. | Valéria Cristina Loureiro Salgado Ronir Raggio Luiz Neio Boechat Bianca C Schorr Isabella S Leao Tiago Nunes Cyrla Zaltman | 2017 | World Journal of Gastroenterology2017，23，30： | 2 |
| 10 | Positron emission tomography scanning in the evaluation of hepatocellular carcinoma显示文摘 | M.Akram Khan Connie S Combs Elizabeth M Brunt Val J Lowe Michael K Wolverson Harvey Solomon Brian T Collins Adrian M.Di Bisceglie | 2000 | Journal of Hepatology2000，，5： | 2 |
| 11 | Oxidative stress promotes pathologic polyploidization in nonalcoholic fatty liver disease显示文摘 | Gentric Géraldine Maillet Vanessa Paradis Valérie Couton Dominique L’Hermitte Antoine Panasyuk Ganna Fromenty Bernard Celton-Morizur Séverine Desdouets Chantal | 2015 | Journal of Clinical Investigation2015，，： | 2 |
| 12 | Early switch to pentoxifylline in patients with severe alcoholic hepatitis is inefficient in non-responders to corticosteroids显示文摘 | Alexandre Louvet Emmanuel Diaz Sébastien Dharancy Hugues Coevoet Frédéric Texier Thierry Thévenot Pierre Deltenre Valérie Canva Christophe Plane Philippe Mathurin | 2007 | Journal of Hepatology2007，，3： | 2 |
| 13 | The role of bacterial vaginosis in preterm labor and preterm birth: A case-control study 显示文摘 | Damien S I Val rie D Fran oise L G | 2002 | Euro J Obstet Gynecol Reprod Biol2002，101，1： | 1 |
| 14 | ldiometric stability of the Multi-angle Imaging SpectroRa- diometer (MISR) following 15 years on-orbit 显示文摘 | Bruegge C J Val S Diner D J | 2014 | Earth Observing Systems2014，9218，92： | 1 |
| 15 | Optimization of mono- and dia-cyglycerols production fromenzymatic glycerolysis in solvent-flee systems显示文摘 | VAL RIO A ROVANI S TREICHEL H | 2010 | Bioprocess Biosyst Eng2010，，33： | 1 |
| 16 | The complexity of interactions of the tumour growth process 显示文摘 | Val~iskov~t Z Kinovh S Danihel L | 2009 | Vnitr Lek2009，55，12： | 1 |
| 17 | The application of pH-stat leaching tests to assess the pH-dependent release of trace metals from soils, sediments and waste materials显示文摘 | Val~rie C Rudy S | 2008 | Journal of Hazardous Materials2008，158，： | 1 |
| 18 | Variations in the major envelope glycoprotein GP5 of Czech strains of porcine reproductive and respiratory syndrome virus显示文摘 | Indik S Valí〖AKcˇ〗ek L Klein D | 2000 | Journal of General Virology2000，81，10： | 1 |
| 19 | Optimisation of wave energy extraction with the Archimedes wave swing显示文摘 | VAL(E)RIO D BEIR(A)O P S(A)DA COSTA J | | 0，，17： | 1 |
| 20 | New macroporous calcium phosphate glass ceramic for guided bone regeneration显示文摘 | Navarro M del Val e S Martinez S | | 0，，18： | 1 |
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