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| 1 | A novel m6A reader Prrc2a controls oligodendroglial specification and myelination显示文摘While N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic mRNA, is linked to cell differentiation and tissue development, the biological significance of m6A modification in mammalian glial development remains unknown. Here, we identify a novel m6A reader, Prrc2a (Proline rich coiled-coil 2A), which controls oligodendrocyte specification and myelination. Nestin-Cre-mediated knockout of Prrc2a induces significant hypomyelination, decreased lifespan, as well as locomotive and cognitive defects in a mouse model. Further analyses reveal that Prrc2a is involved in oligodendrocyte progenitor cells (OPCs) proliferation and oligodendrocyte fate determination. Accordingly, oligodendroglial-lineage specific deletion of Prrc2a causes a similar phenotype of Nestin-Cre-mediated deletion. Combining transcriptome-wide RNA-seq, m6A-RIP-seq and Prrc2a RIP-seq analysis, we find that Olig2 is a critical downstream target gene of Prrc2a in oligodendrocyte development. Furthermore, Prrc2a stabilizes Olig2 mRNA through binding to a consensus GGACU motif in the Olig2 CDS (coding sequence) in an m6A-dependent manner. Interestingly, we also find that the m6A demethylase, Fto, erases the m6A modification of Olig2 mRNA and promotes its degradation. Together, our results indicate that Prrc2a plays an important role in oligodendrocyte specification through functioning as a novel m6A reader. These findings suggest a new avenue for the development of therapeutic strategies for hypomyelination-related neurological diseases. | Rong Wu Ang Li Baofa Sun Jian-Guang Sun Jinhua Zhang Ting Zhang Yusheng Chen Yujie Xiao Yuhao Gao Qingyang Zhang Jun Ma Xin Yang Yajin Liao Wei-Yi Lai Xiaolong Qi Shukun Wang Yousheng Shu Hai-Lin Wang Fengchao Wang Yun-Gui Yang Zengqiang Yuan | 2019 | Cell Research2019，29，1： | 32 |
| 2 | Prevalence of Thyroid Nodules and Its Relationship with Iodine Status in Shanghai:a Population-based Study显示文摘Objective This study was designed to evaluate the prevalence of thyroid nodules(TNs) and its relationship with urine iodine concentrations(UICs) after the regional rapid economic growth and lifestyle changes. Methods A cross-sectional survey was conducted in the general population aged 15-69 years. A questionnaire regarding general and personal characteristics and relevant information was administered. Ultrasonography of the thyroid was performed, and serum triiodothyronine(T3), tetraiodothyronine(T4), serum thyroid stimulating hormone(TSH), free triiodothyronine(FT3), free tetraiodothyronine(FT4), thyroglobulin antibody(Tg Ab), thyroid peroxidase antibody(TPOAb), and TSH receptor antibody(TRAb) levels were measured for each individual subject. Results The prevalence rates of TNs in the whole population, females and males were 27.76%, 34.04%, and 21.60%, respectively. The prevalence of multiple nodules increased with age, whereas the prevalence peaks differed between males and females. The median UICs in the whole population and females with non-TNs were higher than those of subjects with TNs(P=0.0035, P=0.0068). The median UICs in subjects with a single TN were higher than those in subjects with multiple TNs(P=0.0164, P=0.0127). The result showed a U-shaped curve relationship between UIC and prevalence of TNs. The prevalence of TNs was the lowest when the UIC was 140-400 μg/L. Conclusion The prevalence of TNs was nearly 30% and increased with age. The relationship between UIC and prevalence of TNs is U-shaped, with an increase in risk when the UIC was <140 μg/L and >400 μg/L. Very low or high UIC levels need attention and correction. | SONG Jun ZOU Shu Rong GUO Chang Yi ZANG Jia Jie ZHU Zhen Ni MI Ming HUANG Cui Hua YU Hui Ting LU Xi RUAN Ye WU Fan | 2016 | Biomedical and Environmental Sciences2016，29，6： | 25 |
| 3 | Hermetia illucens larvae as a potential dietary protein source altered the microbiota and modulated mucosal immune status in the colon of finishing pigs显示文摘Background: Insects, such as Hermetia illucens larvae, are rich in chitin and proteins, and represent a suitable feed ingredient replacement for animals. However, little is known about the effect of administering H. illucens larvae on intestinal microbiota, bacterial metabolite profiles, and mucosal immune status in animals. This study aimed to investigate the effects of administering H. illucens larvae on colonic microbiota and bacterial metabolites production in finishing pigs. Seventy-two crossbred(Duroc × Landrace × Large White) female pigs(initial body weight, 76.0 ± 0.52 kg) were randomly allocated to three different dietary treatments: a control diet(Control group) and two diets corresponding to 4%(H1 group) and 8%(H2 group) H. illucens larvae inclusion levels, respectively. Each treatment consisted of eight pens(replicates), with three pigs per pen. After 46 days of feeding, eight pigs per treatment(n =8) were slaughtered, and the colonic digesta and mucosa were collected for microbial composition and microbial fermentation products, and genes expression analyses.Results: The results showed that the H1 diet significantly increased the abundance of Lactobacillus,Pseudobutyrivibrio, Roseburia, and Faecalibacterium compared with those in the control group(P < 0.05), with a decrease in the abundance of Streptococcus. The numbers of Lactobacillus, Roseburia, and Clostridium cluster XIVa were significantly greater in the H1 group than in the control group(P < 0.05). Meanwhile, H2 diet increased the number of Clostridium cluster XIVa compared with the control group(P < 0.05). For colonic metabolites, total short chain fatty acids, butyrate, and isobutyrate concentrations were significantly higher in the H1 group than those in the control group(P < 0.05);the H1 treatment caused a striking decrease in protein fermentation compared with the control group, as the concentrations of total amines, cadaverine, tryptamine, phenol, p-cresol, and skatole were significantly lower(P < 0.05). Additionally, H2 diet also increased butyrate concentration compared with control group(P < 0.05), while decreased the concentrations of phenol, p-cresol, and skatole(P < 0.05). Pigs in the H1 group down-regulated the expression of TLR-4 and pro-inflammatory cytokines(IFN-γ) compared with pigs in the control group(P < 0.05), and up-regulated anti-inflammatory cytokine(IL-10) and intestinal barrier genes(ZO-1, occludin, and mucin-1). H2 diet up-regulated the expression of ZO-1 compared with control group(P < 0.05). Furthermore, the changes in the colonic mucosal gene expression were associated with changes in the bacterial composition and their metabolites.Conclusions: Collectively, dietary inclusion of Hermetia illucens larvae may enhance mucosal immune homeostasis of pigs via altering bacterial composition and their metabolites. These findings provide a new perspective on insect meal as a sustainable protein source rich in nutrient ingredients for swine. | Miao Yu Zhenming Li Weidong Chen Ting Rong Gang Wang Xianyong Ma | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 21 |
| 4 | Different dietary starch sources alter the carcass traits, meat quality, and the profile of muscle amino acid and fatty acid in finishing pigs显示文摘Background: With increasing health awareness among consumers, the demand for healthier, tastier, higher quality and nutritional value pork is increasing. It has been shown that different dietary starch sources can alter the carcass traits and meat quality. However, research on the effects of different starch sources with clear different amylose/amylopectin ratio on the amino acid and fatty acid composition in Longissimus thoracis(L. thoracis) muscle of pigs is limited. This study aimed to investigate the effects of different dietary starch sources on carcass traits, meat quality, muscle amino acid and fatty acid composition, and the mRNA expression levels of genes involved in lipid metabolism and muscle fiber characteristics in finishing pigs. A total of 72 Duroc × Landrace × Large White barrows were randomly allocated to 3 different dietary treatment groups with 8 replicate pens/group and 3 pigs per pen.Tapioca starch(TS), corn starch(CS), and pea starch(PS), with amylose/amylopectin ratio of 0.11, 0.25, and 0.44,respectively, were used as their dietary starch sources for 40 days.Results: Results showed that the PS diet significantly increased(P < 0.05) the final body weight, average daily gain,loin-eye area, and fat-free lean index compared with the TS diet, but significantly decreased(P < 0.05) the feed to gain ratio and backfat thickness. Compared with the TS diet, PS diet also increased(P < 0.05) the pH45 min, marbling scores, the content of intramuscular fat and inosine monophosphate in the L. thoracis, and decreased(P < 0.05) the drip loss and shear force. In addition, compared with the TS diet, PS diet increased(P < 0.05) the proportions of flavor amino acids, DHA, EPA, and n-3 polyunsaturated fatty acid(PUFA) in the L. thoracis compared with TS diet,but decreased(P < 0.05) the ratio of n-6/n-3 PUFA. Furthermore, compared with the TS diet, PS diet also upregulated(P < 0.05) the lipogenic genes(FAS, LPL, SCD, ACCα) and myosin heavy-chain(MyHC)-IIa mRNA expression levels compared with the TS diet, but downregulated(P < 0.05) the CPT1 B and MyHC-IIb mRNA levels.Conclusions: In conclusion, these results provided compelling evidence that the different dietary starch source altered the carcass traits, meat flavor and quality in finishing pigs, and consumption of a diet with higher amylose/amylopectin ratio results in the production of a healthy, higher quality, and nutritional value pork. | Miao Yu Zhenming Li Ting Rong Gang Wang Zhichang Liu Weidong Chen Jiazhou Li Jianhao Li Xianyong Ma | 2021 | Journal of Animal Science and Biotechnology2021，12，1： | 13 |
| 5 | Secretory/releasing proteome-based identification of plasma biomarkers in HBV-associated hepatocellular carcinoma显示文摘For successful therapy, hepatocellular carcinoma (HCC) must be detected at an early stage. Herein, we used a proteomic approach to analyze the secretory/releasing proteome of HCC tissues to identify plasma biomarkers. Serum-free conditioned media (CM) were collected from primary cultures of cancerous tissues and surrounding noncancerous tissues. Proteomic analysis of the CM proteins permitted the identification of 1365 proteins. The enriched molecular functions and biological processes of the CM proteins, such as hydrolase activity and catabolic processes, were consistent with the liver being the most important metabolic organ. Moreover, 19% of the proteins were characterized as extracellular or membrane-bound. For validation, secretory proteins involved in transforming growth factor-β signaling pathways were validated in plasma samples. Alphafetoprotein (AFP), metalloproteinase (MMP)1, osteopontin (OPN), and pregnancy-specific beta-1-glycoprotein (PSG)9 were significantly increased in HCC patients. The overall performance of MMP1 and OPN in the diagnosis of HCC remained greater than that of AFP. In addition, this study represents the first report of MMP1 as a biomarker with a higher sensitivity and specificity than AFP. Thus, this study provides a valuable resource of the HCC secretome with the potential to investigate serological biomarkers. MMP1 and OPN could be used as novel biomarkers for the early detection of HCC and to improve the sensitivity of biomarkers compared with AFP. | YANG Lei RONG WeiQi XIAO Ting ZHANG Ying XU Bin LIU Yu WANG LiMing WU Fan QI Jun ZHAO XiuYing WANG HongXia HAN NaiJun GUO SuPing WU JianXiong GAO YanNing CHENG ShuJun | 2013 | Science China(Life Sciences)2013，56，7： | 9 |
| 6 | Comparison of Clinical Efficacy of Three Methods for Cervical Ripening followed by Surgical Evacuation in Early Missed Abortion显示文摘Objective To compare clinical efficacy of three cervical preparations for surgical evacuation in first trimester missed abortion.Methods Two hundred and ten women with early missed abortion were equally randomized into three groups.Three cervical preparations,Dilapan-dilator method(group A,n=70),mifepristone method(group B,n=70),and misoprostol method(group C,n=70),were used 24 h before curettage for terminating missed abortion,respectively.Clinical outcomes and complications for these three methods were compared.Results No differences were found in rates of successful treatment among the three groups(P>0.05).The incidence of fitting number 8 Hegar dilator was slightly high in group A,but it was not different compared with that in group B or group C(P>0.05).Bleeding in previous 24 h in group C was significantly greater than that in group A or group B(P<0.01).The cases of blood loss(>50 ml) during evacuation in group B were more than those in group A(P>0.05).Women in group B had less abdominal pain than those in group A or group C(P<0.01).Side effects in group C were more than those in group A on nausea(P<0.01),vomiting(P<0.05),and diarrhea(P<0.01).Group B had the highest acceptability among the three groups(P<0.05).Conclusion Three methods have the similar clinical efficacies in cervical ripening for surgical treatment of early missed abortion.Based on individual characteristics,different approach would be chosen to avoid some adverse events and improve the clinical application of Dilapan-dilator. | Yu-xia WANG Mi-jiao HUANG Ting HE Rong ZENG Wei-jie ZHU Mei TU | 2012 | Journal of Reproduction and Contraception2012，23，2： | 6 |
| 7 | Inhalation of Roman chamomile essential oil attenuates depressive-like behaviors in Wistar Kyoto rats显示文摘The idea of aromatherapy, using essential oils, has been considered as an alternative antidepressant treatment. In the present study, we investigated the effect of Roman chamomile essential oil inhalation for two weeks on depressive-like behaviors in Wistar-Kyoto(WKY) rats. We found that inhalation of either Roman chamomile or one of its main components α-pinene,attenuated depressive-like behavior in WKY rats in the forced swim test. Using isobaric tags for relative and absolute quantitation analysis(iTRAQ), we found that inhalation of α-pinene increased expression of proteins that are involved in oxidative phosphorylation, such as cytochrome c oxidase subunit 6C-2, cytochrome c oxidase subunit 7A2, ATPase inhibitor in the hippocampus, and cytochrome c oxidase subunit 6C-2, ATP synthase subunit e, Acyl carrier protein, and Cytochrome b-c1 complex subunit 6 in the PFC(prefrontal cortex). In addition, using the quantitative real-time polymerase chain reaction technique, we confirmed an increase of parvalbumin mRNA expression in the hippocampus, which was shown to be upregulated by 2.8-fold in iTRAQ analysis, in α-pinene treated WKY rats. These findings collectively suggest the involvement of mitochondrial functions and parvalbumin-related signaling in the antidepressant effect of α-pinene inhalation. | Yingying Kong Ting Wang Rong Wang Yichuan Ma Shanshan Song Juan Liu Weiwei Hu Shengtian Li | 2017 | Science China(Life Sciences)2017，60，6： | 6 |
| 8 | Post-surgical resection prognostic value of combined OPN, MMP7, and PSG9 plasma biomarkers in hepatocellular carcinoma显示文摘Biomarkers for hepatocellular carcinoma (HCC) following curative resection are not currently sufficient for prognostic indication of overall survival (OS) and disease-free survival (DFS). The aim of this study was to investigate the prognostic performance of osteopontin (OPN), matrix metalloproteinase 7 (MMP7), and pregnancy specific glycoprotein 9 (PSG9) in patients with HCC. A total of 179 prospective patients with HCC provided plasma before hepatectomy. Plasma OPN, MMP7, and PSG9 levels were determined by enzyme-linked immunosorbent assay. Correlations between plasma levels, clinical parameters, and outcomes (OS and DFS) were overall analyzed. High OPN (≥149.97 ng/mL), MMP7 (≥2.28 ng/mL), and PSG9 (≥45.59 ng/mL) were prognostic indicators of reduced OS (P<0.001, P<0.001, and P=0.007, respectively). Plasma PSG9 protein level was an independent factor in predicting OS (P=0.008) and DFS (P=0.038). Plasma OPN+MMP7+PSG9 elevation in combination was a prognostic factor for OS (P<0.001). OPN was demonstrated to be a risk factor-associated OS in stage I patients with HCC and patients with low α-fetoprotein levels (<20 ng/mL). These findings suggested that OPN, MMP7, PSG9 and their combined panels may be useful for aiding in tumor recurrence and mortality risk prediction of patients with HCC, particularly in the early stage of HCC carcinogenesis. | Weiqi Rong Yang Zhang Lei Yang Lin Feng Baojun Wei Fan Wu Liming Wang Yanning Gao Shujun Cheng Jianxiong Wu Ting Xiao | 2019 | Frontiers of Medicine2019，13，2： | 5 |
| 9 | Fargeted elimination of mutant mitochondria DNA in MELAS-iPSCs by mitoTALENs显示文摘 | Yi Yang Han Wu Xiangjin Kang Yanhui Liang Ting Lan Tianjie Li Tao Tan Jiangyun Peng Quanjun Zhang Geng An Yali Liu Qian Yu Zhenglai Ma Ying Lian Boon Seng Soh Qingfeng Chen Ping Liu Yaoyong Chen Xiaofang Sun Rong Li Xiumei Zhen Yang Yu Xiaoping Li Yong Fan | 2018 | Protein & Cell2018，9，3： | 4 |
| 10 | Arctic Climate Changes Based on Historical Simulations(1900-2013) with the CAMS-CSM显示文摘The Chinese Academy of Meteorological Sciences Climate System Model(CAMS-CSM) is a newly developed global climate model that will participate in the Coupled Model Intercomparison Project phase 6. Based on historical simulations(1900-2013), we evaluate the model performance in simulating the observed characteristics of the Arctic climate system, which includes air temperature, precipitation, the Arctic Oscillation(AO), ocean temperature/salinity,the Atlantic meridional overturning circulation(AMOC), snow cover, and sea ice. The model-data comparisons indicate that the CAMS-CSM reproduces spatial patterns of climatological mean air temperature over the Arctic(60°-90°N) and a rapid warming trend from 1979 to 2013. However, the warming trend is overestimated south of the Arctic Circle, implying a subdued Arctic amplification. The distribution of climatological precipitation in the Arctic is broadly captured in the model, whereas it shows limited skills in depicting the overall increasing trend. The AO can be reproduced by the CAMS-CSM in terms of reasonable patterns and variability. Regarding the ocean simulation, the model underestimates the AMOC and zonally averaged ocean temperatures and salinity above a depth of 500 m, and it fails to reproduce the observed increasing trend in the upper ocean heat content in the Arctic. The largescale distribution of the snow cover extent(SCE) in the Northern Hemisphere and the overall decreasing trend in the spring SCE are captured by the CAMS-CSM, while the biased magnitudes exist. Due to the underestimation of the AMOC and the poor quantification of air–sea interaction, the CAMS-CSM overestimates regional sea ice and underestimates the observed decreasing trend in Arctic sea–ice area in September. Overall, the CAMS-CSM reproduces a climatological distribution of the Arctic climate system and general trends from 1979 to 2013 compared with the observations, but it shows limited skills in modeling local trends and interannual variability. | Ting WEI Jian LI Xinyao RONG Wenjie DONG Bingyi WU Minghu DING | 2018 | Journal of Meteorological Research2018，32，6： | 4 |
| 11 | Evaluation of Xpert MTB/RIF for the Diagnosis of Extrapulmonary Tuberculosis in China显示文摘We evaluate the performance of Xpert MTB/RIF for the diagnosis of extrapulmonary tuberculosis(EPTB)in China.The performance of Xpert was evaluated compared to the composite reference standard(CRS),drug susceptibility testing(DST),and imaging examination.The overall sensitivity and specificity of Xpert were 64.1%(195/304)and 100%(24/24),respectively,using CRS as the gold standard.The sensitivity | YUAN Mei LYU Yan CHEN Su Ting CAi Chao LI Yuan ZHANG Zhi Guo LI Yun Xu DONG Ling Ling FU Yu Hong HUANG Hai Rong GAO Ji Min LI Wei Min | 2016 | Biomedical and Environmental Sciences2016，29，8： | 3 |
| 12 | Three Gorges Dam: friend or foe of riverine greenhouse gases?显示文摘Dams are often regarded as greenhouse gas(GHG) emitters. However, our study indicated that the world’s largest dam, the Three Gorges Dam(TGD), has caused significant drops in annual average emissions of CO, CHand NO over 4300 km along the Yangtze River, accompanied by remarkable reductions in the annual export of CO(79%), CH(50%) and NO(9%) to the sea. Since the commencement of its operation in 2003, the TGD has altered the carbonate equilibrium in the reservoir area, enhanced methanogenesis in the upstream, and restrained methanogenesis and denitrification via modifying anoxic habitats through long-distance scouring in the downstream. These findings suggest that ‘large-dam effects’ are far beyond our previous understanding spatiotemporally, which highlights the fundamental importance of whole-system budgeting of GHGs under the profound impacts of huge dams. | Jinren Ni Haizhen Wang Tao Ma Rong Huang Philippe Ciais Zhe Li Yao Yue Jinfeng Chen Bin Li Yuchun Wang Maosheng Zheng Ting Wang Alistair G.L.Borthwick | 2022 | National Science Review2022，9，6： | 3 |
| 13 | PSG9 promotes angiogenesis by stimulating VEGFA production and is associated with poor prognosis in hepatocellular carcinoma显示文摘Hepatocellular carcinoma(HCC) is a common malignant solid tumor characterized by rich vascularization. Pregnancy-specific glycoprotein 9(PSG9) is a member of the carcinoembryonic antigen(CEA)/PSG family and is produced at high levels during pregnancy. We previously identified PSG9 as an HCC-related protein. However, the expression of PSG9 and its regulation during HCC carcinogenesis remain poorly explored. In the present study, we first found that the levels of PSG9 protein were significantly increased in the plasma of HCC patients. PSG9 overexpression also increased the proliferation ability of an HCC cell line. High expression of PSG9 was associated with angiogenesis by accelerating VEGFA expression. In addition, Cox's proportional hazards model analysis revealed that the plasma level of PSG9 was an independent prognostic factor for overall survival. We propose that PSG9 is a novel indicator of prognosis in patients with HCC and could serve as a novel therapeutic target for HCC. Furthermore,our results indicate that PSG9 protein may facilitate the development of HCC by fostering angiogenesis via promoting VEGFA production in cancer cells. | Weiqi Rong Lei Yang Liyuan Yin Yanning Gao Ting Xiao Shujun Cheng | 2017 | Science China(Life Sciences)2017，60，5： | 3 |
| 14 | dentification of DW532 as a novel anti-tumor agent targeting both kinases and tubulin显示文摘 | Ting PENG Jian-rui WU Lin-jiang TONG Meng-yuan LI Fang CHEN Yi-xin LENG Rong QU Kun HAN Yi SU Yi CHEN Wen-hu DUAN Hua XIE Jian DING | 2014 | Acta Pharmacologica Sinica2014，35，7： | 3 |
| 15 | Synergistic catalysis of isolated Fe^(3+) and Fe_2O_3 on FeO_x/HZSM-5 catalysts for Friedel-Crafts benzylation of benzene显示文摘FeO_x/HZSM-5 catalyst with 8 wt.%Fe-loading(8-FeZ) exhibited significantly higher reactivity in the benzylation of benzene with benzyl chloride than FeO_x/HZSM-5 catalyst with 2.5 wt.%Fe-loading(2.5-FeZ) because the synergistic catalysis between isolated Fe^(3+) and superfine Fe_2O_3 occurred on 8-FeZ in the reaction. | Tao Lin Xin Zhang Rong Li Ting Bai Si Ying Yang | 2011 | Chinese Chemical Letters2011，22，6： | 3 |
| 16 | Markov Chain-based Degree Distributions of Evolving Networks显示文摘In this paper, we study a class of stochastic processes, called evolving network Markov chains, in evolving networks. Our approach is to transform the degree distribution problem of an evolving network to a corresponding problem of evolving network Markov chains. We investigate the evolving network Markov chains, thereby obtaining some exact formulas as well as a precise criterion for determining whether the steady degree distribution of the evolving network is a power-law or not. With this new method, we finally obtain a rigorous, exact and unified solution of the steady degree distribution of the evolving network. | Xiang Xing KONG Zhen Ting HOU Ding Hua SHI Quan Rong CHEN Qing Gui ZHAO | 2012 | Acta Mathematica Sinica,English Series2012，28，10： | 2 |
| 17 | Inhibition of KU70 and KU80 by CRISPR interference,not NgAgo interference,increases the efficiency of homologous recombination in pig fetal fibroblasts显示文摘Non-homologous end-joining(NHEJ) is a predominant pathway for the repair of DNA double-strand breaks(DSB). It inhibits the efficiency of homologous recombination(HR) by competing for DSB targets. To improve the efficiency of HR, multiple CRISPR interference(CRISPRi) and Natronobacterium gregoryi Argonaute(NgAgo) interference(NgAgoi) systems have been designed for the knockdown of NHEJ key molecules, KU70, KU80, polynucleotide kinase/phosphatase(PNKP), DNA ligase IV(LIG4), and NHEJ1. Suppression of KU70 and KU80 by CRISPRi dramatically promoted(P<0.05) the efficiency of HR to 1.85-and 1.58-fold, respectively, whereas knockdown of PNKP, LIG4, and NHEJ1 repair factors did not significantly increase(P>0.05) HR efficiency. Interestingly, although the NgAgoi system significantly suppressed(P<0.05) KU70, KU80, PNKP, LIG4, and NHEJ1 expression, it did not improve(P>0.05) HR efficiency in primary fetal fibroblasts. Our result showed that both NgAgo and catalytically inactive Cas9(dCas9) could interfere with the expression of target genes, but the downstream factors appear to be more active following CRISPR-mediated interference than that of NgAgo. | LI Guo-ling QUAN Rong WANG Hao-qiang RUAN Xiao-fang MO Jian-xin ZHONG Cui-li YANG Huaqiang LI Zi-cong GU Ting LIU De-wu WU Zhen-fang CAI Geng-yuan ZHANG Xian-wei | 2019 | Journal of Integrative Agriculture2019，18，2： | 2 |
| 18 | A glutathione activatable pro-drug-photosensitizer for combined chemotherapy and photodynamic therapy显示文摘During cancer treatment,chemotherapeutic drugs always result in severe side-effects and drug resistance.Therefore,combining cheomtherapy with other therapeutic modalities,such as photodynamic therapy(PDT)and designing an activable platform is promising for precise and efficient anticancer treatment.Herein,we report a“pro-drug-photosensitizer”agent,LMB-S-CPT,bearing a disulfide bond as the glutathione(GSH)-activatable linker.LMB-S-CPT can be selectively activated by GSH to release activated drug,camptothecin(CPT),for chemotherapy and activated photosensitizer,methylene blue(MB),for PDT.LMB-S-CPT exhibits excellent tumor-activatable performance when injected into tumor-bearing mice,as well as specific cancer therapy with negligible toxic side effects.The activatable pro-drug-photosensitizer offers a new strategy for chemo-photodynamic therapy and displays precise,selective and excellent antitumor effect. | Yanjun Yang Yifeng Zhang Ran Wang Xiang Rong Ting Liu Xiang Xia Jiangli Fan Wen Sun Xiaojun Peng | 2022 | Chinese Chemical Letters2022，33，10： | 2 |
| 19 | Numerical Simulation of Jet Behavior and Impingement Characteristics of Preheating Shrouded Supersonic Jets显示文摘As a novel supersonic jet technology,preheating shrouded supersonic jet was developed to deliver oxygen into molten bath efficiently and affordably.However,there has been limited research on the jet behavior and impingement characteristics of preheating shrouded supersonic jets.Computational fluid dynamics(CFD)models were established to investigate the effects of main and shrouding gas temperatures on the characteristics of flow field and impingement of shrouded supersonic jet.The preheating shrouded supersonic jet behavior was simulated and measured by numerical simulation and jet measurement experiment respectively.The influence of preheating shrouded supersonic jet on gas jet penetration and fluid flow in liquid bath was calculated by the CFD model which was validated against water model experiments.The results show that the uptrend of the potential core length of shrouded supersonic jet would be accelerated with increasing the main and shrouding gas temperatures.Also,preheating supersonic jets demonstrated significant advantages in penetrating and stirring the liquid bath. | Guang-sheng WEI Rong ZHU Ting CHENG Fei ZHAO | 2016 | Journal of Iron and Steel Research(International)2016，23，10： | 2 |
| 20 | LiFePO_4/C via fluoride doping显示文摘Non-stoichiometric compound fluoride-doped LiFePO4/C cathode materials were synthesized via solid-state reaction using MgF2 and AlF3 as dopant. The fluoride-doped LiFePO4/C samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and electrochemical testing. The results show that the materials are well crystallized and fluoride doping cannot change the space structure of LiFePO4. Slight amounts of Fe2O3 with no fluoride impurity were detected. Charge-discharge curves show that fluoride-doped samples have higher capacity at low rates compared with undoped LiFePO4/C. AlF3-doped samples have highest capacity at high discharge current. Both doped samples have larger polarization voltage than undoped samples. All samples exhibit good cycle stability. | Gu Yuan Zhang Xiangjun Lu Shigang Zhao Ting Jiang Danping Yang Rong Wu Aide | 2012 | Rare Metals2012，31，6： | 2 |
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