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| 1 | A novel m6A reader Prrc2a controls oligodendroglial specification and myelination显示文摘While N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic mRNA, is linked to cell differentiation and tissue development, the biological significance of m6A modification in mammalian glial development remains unknown. Here, we identify a novel m6A reader, Prrc2a (Proline rich coiled-coil 2A), which controls oligodendrocyte specification and myelination. Nestin-Cre-mediated knockout of Prrc2a induces significant hypomyelination, decreased lifespan, as well as locomotive and cognitive defects in a mouse model. Further analyses reveal that Prrc2a is involved in oligodendrocyte progenitor cells (OPCs) proliferation and oligodendrocyte fate determination. Accordingly, oligodendroglial-lineage specific deletion of Prrc2a causes a similar phenotype of Nestin-Cre-mediated deletion. Combining transcriptome-wide RNA-seq, m6A-RIP-seq and Prrc2a RIP-seq analysis, we find that Olig2 is a critical downstream target gene of Prrc2a in oligodendrocyte development. Furthermore, Prrc2a stabilizes Olig2 mRNA through binding to a consensus GGACU motif in the Olig2 CDS (coding sequence) in an m6A-dependent manner. Interestingly, we also find that the m6A demethylase, Fto, erases the m6A modification of Olig2 mRNA and promotes its degradation. Together, our results indicate that Prrc2a plays an important role in oligodendrocyte specification through functioning as a novel m6A reader. These findings suggest a new avenue for the development of therapeutic strategies for hypomyelination-related neurological diseases. | Rong Wu Ang Li Baofa Sun Jian-Guang Sun Jinhua Zhang Ting Zhang Yusheng Chen Yujie Xiao Yuhao Gao Qingyang Zhang Jun Ma Xin Yang Yajin Liao Wei-Yi Lai Xiaolong Qi Shukun Wang Yousheng Shu Hai-Lin Wang Fengchao Wang Yun-Gui Yang Zengqiang Yuan | 2019 | Cell Research2019，29，1： | 32 |
| 2 | Hyperbaric oxygen therapy improves cognitive functioning after brain injury显示文摘Hyperbaric oxygen therapy has been widely applied and recognized in the treatment of brain injury;however,the correlation between the protective effect of hyperbaric oxygen therapy and changes of metabolites in the brain remains unclear.To investigate the effect and potential mechanism of hyperbaric oxygen therapy on cognitive functioning in rats,we established traumatic brain injury models using Feeney’s free falling method.We treated rat models with hyperbaric oxygen therapy at 0.2 MPa for 60 minutes per day.The Morris water maze test for spatial navigation showed that the average escape latency was significantly prolonged and cognitive function decreased in rats with brain injury.After treatment with hyperbaric oxygen therapy for 1 and 2 weeks,the rats’spatial learning and memory abilities were improved.Hydrogen proton magnetic resonance spectroscopy analysis showed that the N-acetylaspartate/creatine ratio in the hippocampal CA3 region was significantly increased at 1 week,and the N-acetylaspartate/choline ratio was significantly increased at2 weeks after hyperbaric oxygen therapy.Nissl staining and immunohistochemical staining showed that the number of nerve cells and Nissl bodies in the hippocampal CA3 region was significantly increased,and glial fibrillary acidic protein positive cells were decreased after a 2-week hyperbaric oxygen therapy treatment.Our findings indicate that hyperbaric oxygen therapy significantly improves cognitive functioning in rats with traumatic brain injury,and the potential mechanism is mediated by metabolic changes and nerve cell restoration in the hippocampal CA3 region. | Su Liu Guangyu Shen Shukun Deng Xiubin Wang Qinfeng Wu Aisong Guo | 2013 | Neural Regeneration Research2013，8，35： | 16 |
| 3 | A case-control study of risk factors for severe hand-foot-mouth disease in Yuxi, China, 2010–2012显示文摘Dear Editor,Here,we report the risk factors for severe hand-foot-mouth disease(HFMD)determined by our case-controlstudy.Our findings could help disease prevention and in-tervention initiatives.Patients with severe HFMD displayfatal clinical manifestations with sequelae,requiring≥7days of hospitalization.A tota1 of 249 severe cases treat-ed at Yuxi Children’s Hospital were included in the | Ze Liu Shukun Wang Rusong Yang Xia Ou | 2014 | Virologica Sinica2014，29，2： | 14 |
| 4 | WEAR FAILURE MECHANISM AND MULTI-IMPACT PROPERTY OF AUTOMOTIVE ENGINE CHAIN显示文摘The multi-impact characteristics and failure mechanism of two kinds of automotive enginechain made in China are studied through engine assembly and road-drive tests. The worn surfacemorphologies of rubbing area between pin, bush and roller are also analyzed based on scanning elec-tron microscope. The results show that the main wear mechanism of automotive engine chain is fa-tigue wear, and its failure mechanism is the forming, extending and flaking of cracks on top layer ofpin and bush. In addition, the material, hot-treatment method and shaping technique for roller have agreat influence upon the resistance to multi-impact. Ensuring sufficient strength and plasticity ofroller, as well as adopting suitable shaping technique are the effective method to increase its resistanceto multi-impact. | Meng Fanzhong Wang Shukun Lan Hong Xu Rongjin Xu Hanxue | 2005 | Chinese Journal of Mechanical Engineering2005，18，3： | 12 |
| 5 | Large numbersLarge numbers of new bacterial taxa found by Yunnan Institute of Microbiology显示文摘Extreme environments, primeval forest, sea sediments and plants in China yielded members of a new suborder, Jiangellineae, a new family Sinobacteraceae, and 23 new genera of actinomycetes and other bacteria. Three suggestions were made for further discoveries of new actinomycetes. First, natural habitats such as old growth forests, and extreme environments, should be given sampling priority; second, culturing procedures need to be constantly improved to mimic natural habitats; third, less than 98.5% similarity of 16S rRNA sequence to that of a known species could be used as an indicator of a new species. | JIANG Yi CAO YanRu ZHAO LiXing TANG ShuKun WANG Yun LI WenJun XU Ping LOU Kai MAO PeiHong XU LiHua | 2011 | Chinese Science Bulletin2011，56，8： | 5 |
| 6 | 控制气管插管套囊压力与术后并发症关系的多中心研究显示文摘背景与气管插管相关的术后呼吸系统并发症通常表现为咳嗽、咽喉痛、声嘶、痰中带血。该研究中,我们研究了短时间(数小时)测量和控制气管插管套囊(endotracheal tube cuff,ETrc)压力对术后并发症的影响。方法选择中国上海4所大学附属三级医院拟在全身麻醉下行择期手术的患者509例,分为不测量ETTc压力的对照组和测量并调整ETTc压力的研究组,记录手术时间和插管时间。每组选择插管时间在120—180分钟的20例患者,拔管后即刻行纤维支气管镜检查。拔管后24小时记录咳嗽、咽喉痛、声嘶、痰中带血等气管插管相关并发症。结果两组间性别、年龄、身高、体重、手术时间和插管时问均无显著性差异。研究组调整前通过触摸指示气囊估计的平均ETTc压力为43±23.3mmHg(最高210mmHg),调整后为20±3.1mmHg(P〈0.001)。对照组术后咽喉痛、声嘶、痰中带血的发生率明显高于研究组。随着插管时间的增加,对照组中咽喉痛和痰中带血的发生率增加。研究组中咽喉痛的发生率也随插管时间增加而增加。对40例患者的纤维支气管镜检查结果显示支气管黏膜均有不同程度的损伤,对照组的损伤比研究组更严重。结论依据个人经验通过触捏估计ETTc压力明显高于测量结果或最适压力。即使在短时间(1—3小时)手术中,通过压力计合理控制ETTc压也有助于减少E]丌相关的咳嗽、咽喉痛、声嘶、痰中带血等术后呼吸系统并发症。 | Jianhui Liu Xiaoqing Zhang Wei Gong Shitong Li Fen Wang Shukun Fu Mazhong Zhang Yannan Hang 安博(译) 杜金(校) | 2013 | 麻醉与镇痛2013，，1： | 3 |
| 7 | Changes in extreme events of temperature and precipitation over Xinjiang, northwest China, during 1960–2009显示文摘 | Baolong Wang Mingjun Zhang Junlin Wei Shengjie Wang Shanshan Li Qian Ma Xiaofei Li Shukun Pan | 2012 | Quaternary International2012，，： | 1 |
| 8 | 来源于人膀胱癌细胞株EJ的HSP70负载的DC所激发的特异性CTL细胞的体外应答效应(英文)显示文摘Objective: To investigate whether human dendritic cells (DC) derived from peripheral blood mononuclear cells (PBMC), which were pulsed by heat shock protein 70 (HSP70) isolated from human bladder tumor cell lines of EJ, were able to induce peptide specific cytotoxic T-lymphocytes (CTL) response in vitro and give the experimental foundation for the future clinical trials of immunotherapy in bladder tumor. Methods: The EJ-derived HSP70 co-cultured with DC from the healthy volunteers' PBMC, along with the crude lysate (the supernatant before HSP70 purification) from EJ cells were used as the experimental groups and DC not pulsed by any tumor cells antigen were the blank control. The autologous T-lymphocytes were added into the above various DC groups, and after incubation, the stimulation indexes (SI) and interferon-γ (IFN-γ) were detected to evaluate the immune activities of various DC groups. The killing effects of CTL to target cells, EJ and Hela cells, were determined with 51Cr releasing test. Results: Both DC/HSP70 and DC/the crude lysate could effectively activate CTL in vitro and kill target cells EJ. The killing effect of DC/HSP70 to EJ was much stronger than DC/the crude lysate (the supernatant before HSP70 purification) (P < 0.05). DC without any tumor cell antigens had a lower killing power to EJ. Meanwhile, DC/ HSP70 had little killing power to Hela non-relevant to bladder tumor histopathologically as compared with EJ cells (P < 0.05). Conclusion: The DC pulsed by HSP70 derived from the autologous tumor cells could induce a peptide complexes specific CTL response to tumor cells, and the CTL response induced by the DC/HSP70 was stronger, which display the basis of the possible clinical application of DC/HSP70 for bladder tumor. | Lingfeng He Jianhua Wang Xiaofeng Wang Xiangjun He Zheng Yan Kexin Xu Kaopeng Guan Shukun Hou | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，12： | 1 |
| 9 | Visceral sensitivity to gastric stimulation and its correlation with alterations in gastric emptying and accommodation in humans 显示文摘 | Shukun Yao Meiyun Ke Zhifeng Wang | 2005 | Obesity Surgery2005，15，： | 1 |
| 10 | Changes in extreme events of temperature and precipitation over Xinjiang, northwest China, during 1960–2009显示文摘 | Baolong Wang Mingjun Zhang Junlin Wei Shengjie Wang Shanshan Li Qian Ma Xiaofei Li Shukun Pan | 2012 | Quaternary International2012，，： | 1 |
| 11 | Study on blood cultures and bacteria counts in blood of paratyphoid fever A patients显示文摘 | SHUKUN WANG CONGJIA CHU PEISHI SUN | 2009 | Eur J Clin Mierobiol Infect Dis2009，28，10： | 1 |
| 12 | Oligomerization and Polymerization of Ethylene Initiated by a Novel Ni(Ⅱ)-Based Acetyliminopyridine Complexes as Single-Site Catalysts显示文摘新奇的镍( II )基于 acetyliminopyridine 建筑群 1b , 2b , 3b ( 1 ¨C 3b )从 ligands 1a 被综合, 2a , 3a ( 1-3a )并且[ NiCl2 ( DME )],是为为乙烯 oligomerization 和聚合反应是必要的催化剂的合适的先锋,由 methylaluminoxane (毛)激活。对待 MAO 1 ¨ C 3b 介绍一个活跃催化中心,它可以分别地生产线性 | á - 石蜡和聚乙烯的 oligomerize 和聚合乙烯。被获得的 oligomers 的分子量分布在对 oligomers 的 Schulz-Flory 规则的好同意 > C4。3b-MAO 建筑群的活动是 6.3 ? á 10 (7 ) 在 50 点的 g/(molNi-h )??6 号元素碳的化学符号。oligomers 的活动和分子量分布在催化剂先锋的结构上显示出重要信赖。给词调音:oligomerization;聚合;乙烯;镍(II ) 基于催化剂;| á - | Baojun Zhang Yanji Wang Gang Wang Jun Cao Shukun Sun Lihua Xing Yongcheng Sun Yunguang Han | 2007 | Journal of Natural Gas Chemistry2007，16，1： | 1 |
| 13 | A physical model study of surrounding rock failure near a fault under the influence of footwall coal mining显示文摘A study of the deformation of the surrounding rock and coal pillars near a fault under the influence of mining is conducted on a physical model for the design of coal pillars to support and maintain the roofs of adjacent fault roadways.This research is based on the 15101 mining face in the Baiyangling Coal Mine,Shanxi,China,and uses simulation tests similar to digital speckle test technology to analyse the displacement,strain and vertical stress fields of surrounding rocks near faults to determine the influence of the coal pillar width.The results are as follows.The surrounding rock of the roadway roof fails to form a balance hinge for the massive rock mass.The vertical displacement,vertical strain and other deformation of the surrounding rock near the fault increase steeply as the coal pillar width decreases.The steep increase in deformation corresponds to a coal pillar width of 10 m.When the coal pillar width is 7.5 m,the pressure on the surrounding rock near the footwall of the fault suddenly increases,while the pressure on the hanging wall near the fault increases by only 0.35 MPa.The stress of the rock mass of the hanging wall is not completely shielded by the fault,and part of the load disturbance is still transmitted to the hanging wall via friction.The width of the fault coal pillars at the 15101 working face is determined to be 7.5 m,and the monitoring data verify the rationality of the fault coal pillars. | Shukun Zhang Lu Lu Ziming Wang Shuda Wang | 2021 | International Journal of Coal Science & Technology2021，8，4： | 1 |
| 14 | Synthesis,growth and spectral properties of Tm3+/Yb3+ -doped YVO4 crystal显示文摘 | Zhang Lizhen Wang Guofu Lin Shukun | 2002 | Journal of Crystal Growth2002，241，： | 1 |
| 15 | Spatial-Temporal Variation Characteristics and Cause Analysis of Water Quality in Yinma River Basin显示文摘In order to comprehensively understand the water quality in Yinma River Basin,and to analyze the spatial-temporal variation characteristics of water quality over the years and the causes of water pollution,15 sections were selected in the Yinma River Basin.Based on the water quality monitoring data in the past decade,7 evaluation indexes including dissolved oxygen(DO),5-day biochemical oxygen demand(BOD_(5)),permanganate index(COD_(MN)),chemical oxygen demand(COD),ammonia nitrogen(NH_(4)-N),total phosphorus(TP)and total nitrogen(TN)were determined,according to the Environmental Quality Standard for Surface Water(GB3838-2002)and the historical monitoring data of rivers.The water quality of 15 sections in Yinma River Basin was comprehensively evaluated,and the water quality categories and the evolution of river water quality were analyzed.The pollution sources of Yinma River Basin were analyzed,and the constructive countermeasures were put forward according to the conclusions. | Xiaolai WU Jing WANG Shukun JIANG LeiXU Xiangzhe MENG | 2022 | Asian Agricultural Research2022，14，4： | 0 |
| 16 | Spatiotemporal Variation Characteristics of Water Quality in the Yinma River显示文摘As an important river in the central part of Jilin Province, the Yinma River plays a crucial role in the daily lives of the people in Jilin Province. In this paper, 15 cross sections were selected in the Yinma River basin. Based on the water quality monitoring data from 2012 to 2021, referring to the Environmental Quality Standards for Surface Water (GB 3838-2002) and historical monitoring data of the river, dissolved oxygen (DO), five-day biochemical oxygen demand (BOD 5), permanganate index (COD Mn ), chemical oxygen demand (COD), ammonia nitrogen (NH 4-N), total phosphorus (TP), and total nitrogen (TN) were determined as 7 evaluation indicators, and the water quality of 15 cross sections in the Yinma River basin was comprehensively evaluated. A characteristic analysis was conducted on the water quality of the Yinma River, and its pollution sources were identified. Based on the conclusions, constructive control measures were proposed. | Shukun JIANG Jing WANG Yinglin XIE Chunsheng WEI Xin TIAN Lunwen WANG | 2023 | Meteorological and Environmental Research2023，14，6： | 0 |
| 17 | Prevalence, Phenotypes and Virulence Factors Characteristics of Escherichia coli O157 strains in Yuxi City显示文摘 | WANG Shukun PANG Bo JING Huaiqi XIANG Zhenghua YAO Yunbo LIU Hongyan LI Shunxiang | 2005 | 美国中华健康卫生杂志2005，8，4： | 0 |
| 18 | Sudden cardiac death of Duchenne muscular dystrophy with NT-proBNP in pericardial fluid as a useful biomarker for diagnosis of the cause of death: a case report显示文摘Duchenne muscular dystrophy(DMD)is one of the most common and severest muscular dystrophies.Although it can be a cause of death when associated with cardiac muscle and/or respiratory muscles,no cases of sudden deaths in the setting of undiagnosed DMD with cardiac involvement have been reported in the literatures.Previous studies showed that N-terminal-proBNP(NT-proBNP)was a robust laboratory biomarker to diagnose and monitor cardiac failure in clinical situations,suggesting that it may be used as an auxiliary indicator for diagnosis on left ventricular dysfunction in sudden cardiac deaths in forensic settings.Here,we reported a case of 29-year-old man who died suddenly.Autopsy revealed that muscles of the body were almost replaced by fatty and fibrotic tissues.The heart was enlarged with disarray and degeneration of cardiomyocytes in cardiac muscle.Total absence of dystrophin was detected by immunohistochemical staining,which confirmed DMD.Postmortem biochemical test of pericardial fluid revealed a high level of NT-proBNP,indicating dysfunction of the left ventricle before death.The cause of death was certified as an early dilated cardiomyopathy(DCM)/dysfunction of the left ventricle secondary to DMD,suggesting that sudden cardiac death with cardiac dysfunction could be identified by immunohistochemical method in combination with pericardial fluid NT-proBNP determination after systemic autopsy. | Mengzhou Zhang Rui Zhao Tianshui Yu Jiaoyong Li Miao Zhang Shukun Jiang Linlin Wang Guohua Zhang Rubo Li Baoli Zhu Dawei Guan | 2020 | Forensic Sciences Research2020，5，2： | 0 |
| 19 | Review and Prospect of Development Capability of New Generation Farmers显示文摘In the context of dual economic structure,there occurs shift of numerous rural surplus labors in China. As a special group lingering about in the edges of urban and rural areas,the new generation farmers become a hot spot of research. How to help them smoothly become successors of China's agricultural modernization construction is a focus of academic circles. Based on literature review,this paper analyzed connotation of development capability of new generation farmers from agricultural modernization,thinking ability construction,system and policy,and new farmers. Finally,it came up with prospect for development of new generation farmers. | Shukun WANG Shaojun CHEN | 2015 | Asian Agricultural Research2015，7，11： | 0 |
| 20 | Biological Evaluation of Water Quality for Benthos in Typical Rivers of Eastern Jilin Province显示文摘Benthos are aquatic organisms living at the bottom of water bodies for all or most of their life history.Apart from the settled and moving living,their forms of habitat are mostly attached to the hard substrate like rocks or soft bases such as mud and sand.In addition,there are benthic species that attach to the plant surfaces or other benthic animals,and inhabit the intertidal zone.In terms of feeding methods,most benthic animals feed on suspended matter and sediment.Most of them are invertebrates and are a complex ecological group.According to the size,they can be divided into macrobenthos and meio-benthos[1].Benthos are an important part of the water ecosystem.Using a typical river(Gaya River)in eastern Jilin Province,this paper analyzed the water quality,the components of benthic organisms and their biological density through sampling surveys of three typical locations in the upper,middle and lower reaches of the Gaya River.In addition,it made a biological evaluation of the water quality of the Gaya River. | Lei XU Jing WANG Xiaolai WU Shukun JIANG Chunsheng WEI | 2021 | Asian Agricultural Research2021，13，7： | 0 |
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