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| 1 | 5-Methylcytosine RNA Methylation in Arabidopsis Thaliana显示文摘5-Methylcytosine (m 5 C ) 是描绘得好的 DNA 修正,并且主要也在初核质和优核质在丰富的非编码的 RNA 被报导。然而,分发和 m 5 在植物 mRNAs 的 C 仍然保持大部分未知。这里,我们报导 RNA m 5 在由使用 m 5 C RNA immunoprecipitation 由一条深定序的途径列在后面(m 5 C-RIP-seq ) 。LC-MS/MS 和点污点分析揭示 m 5 在各种各样的纸巾并且在不同发展阶段的 C mRNA 修正。m 5 C-RIP-seq 分析识别了 6045 m 5 在 4465 的 C 山峰在年轻幼苗表示了基因。我们发现那 m 5 C 在编码序列,二座山峰在开始 codons 以后并且在站 codons 前立即定位了被充实,并且随低翻译与 mRNAs 被联系活动。我们进一步表明了那 RNA (cytosine-5 )-methyltransferase,tRNA 特定的 methyltransferase 4B (TRM4B ) ,展览 m 5 C RNA methyltransferase 活动。在 TRM4B 的变化在根开发和减少的 m 5 C 山峰。TRM4B 影响涉及根开发的基因的抄本层次,它断然与他们的 mRNA 稳定性和 m 5 C 层次。我们的结果建议那 m 5 在 mRNA 的 C 是新 epitranscriptome 标记 inArabidopsis,并且这修正的那条规定是基因的不可分的部分规章的网络位于 \O 下面植物开发。 | Xuean Cui Zhe Liang Lisha Shen Qian Zhang Shengjie Bao Yuke Geng Bin Zhang Vonny Leo Leah A. Vardy Tiegang Lu Xiaofeng Gu Hao Yu | 2017 | Molecular Plant2017，10，11： | 19 |
| 2 | N6-Methyladenine DNA Methylation in Japonica and Indica Rice Genomes and Its Association with Gene Expression,Plant Development, and Stress Responses显示文摘N6-Methyladenine (6mA)DNA methylation has recently been implicated as a potential new epigenetic marker in eukaryotes,including the dioot modelArabidopsis thaliana.However,the conservation and divergence of 6mA distribution patterns and functions in plants remain elusive.Here we report high-quality 6mA methylomes at single-nucleotide resolution in rice based on substantially improved genome sequences of two rice cultivars,Nipponbare (Nip;Japonica)and 93-11 (Indica).Analysis of 6mA genomic distribution and its association with transcription suggest that 6mA distribution and function is rather conserved between rice and Arabidopsis.We found that 6mA levels are positively correlated with the expression of key stressrelated genes,which may be responsible for the difference in stress tolerance between Nip and 93-11. Moreover,we showed that mutations in DDM1 cause defects in plant growth and decreased 6mA level. Our results reveal that 6mA is a conserved DNA modification that is positively associated with gene expression and contributes to key agronomic traits in plants. | Qian Zhang Zhe Liang Xuean Cui Changmian Ji Yun Li Pingxian Zhang Jingrong Liu Adeel Riaz Pu Yao Min Liu Yunpeng Wang Tiegang Lu Hao Yu Donglei Yang Hongkun Zheng Xiaofeng Gu | 2018 | Molecular Plant2018，11，12： | 18 |
| 3 | The RNA Editing Factor WSP1 Is Essentia for Chloroplast Development in Rice显示文摘尽管在 Arabidopsis thaliana 的质体编辑因素(MORF ) 的多重 organellar RNA 是位于下面 MORF 怎么在另外的种类影响植物开发的广泛地学习的、分子的细节,特别地在米饭,仍然保持大部分未知。这里,我们描述 wsp1 的描述,有白条纹的叶子和圆锥花序的米饭异种。尤其是, wsp1 在出发舞台展出了将近白的不成熟的圆锥花序。传播电子显微镜学分析和叶绿素内容测量揭示了叶绿体在 wsp1 的发展缺点和减少的叶绿素累积。在 Os04g51280 发现一个点变化的 WSP1 的位置的克隆,份额其通常认为的产品高与 MORF 蛋白质定序类似。互补实验证明 WSP1 为 wsp1 的使成杂色的显型负责。WSP1 对叶绿体局部性,在 wsp1 的点变化影响编辑多重 organellar RNA 地点。由于在编辑的质体 RNA 的缺点,叶绿体核糖体生物的续生说并且 ndhA 拼接也在 wsp1 被损害,它可以在出发阶段在叶子和圆锥花序影响正常叶绿体开发。一起,我们的结果在叶绿体开发表明米饭 WSP1 蛋白质的重要性并且在米饭关于 MORF 家庭成员拓宽我们的知识。 | Zhiguo Zhang Xuean Cui Yanwei Wang JinxiaWu Xiaofeng Gu Tiegang Lu | 2017 | Molecular Plant2017，10，1： | 15 |
| 4 | Bevacizumab biosimilar LY01008 compared with bevacizumab(Avastin)as first-line treatment for Chinese patients with unresectable,metastatic,or recurrent non-squamous non-small-cell lung cancer:A multicenter,randomized,double-blinded,phase Ⅲ trial显示文摘Background:Previous studies have demonstrated the preclinical pharmacological and toxicological consistency,and clinical pharmacokinetic equivalence of bevacizumab biosimilar LY01008 with reference bevacizumab(Avastin).This randomized controlled trial aimed to compare the efficacy and safety of LY01008 with Avastin in first-line treatment of Chinese patients with advanced or recurrent non-squamous non-small cell lung cancer(NSCLC).Methods:StageⅢB-ⅣNSCLC patients with evaluable lesions,good physical status,and adequate organ functions from 67 centers across China were randomized in a ratio of 1:1 to receive LY01008 or Avastin 15 mg/kg intravenously in combination with paclitaxel/carboplatin(combined treatment)for 4-6 cycles,followed by maintenance monotherapy with LY01008 until disease progression,intolerable toxicity,or death.The primary endpoint was objective response rate(ORR)in accordance with Response Evaluation Criteria in Solid Tumors(RECIST)version 1.1 confirmed by independent radiological review committees(IRRC).Secondary endpoints included disease control rate(DCR),duration of response(DoR),progression-free survival(PFS),overall survival(OS),and safety.This study was registered in Clinical Trials.gov(NCT03533127).Results:Between December 15^(th),2017,and May 15^(th),2019,a total of 649 patients were randomized to the LY01008(n=324)or Avastin(n=325)group.As of September 25th,2019 for primary endpoint analysis,589 patients received ORR evaluation,with a median number of combined treatment cycles of 5(range 1-6)andmedian duration of treatment of 3.0(range 0.0-5.1)months.ORRof responseevaluable patients in the LY01008 and Avastin groups were 48.5% and 53.0%,respectively.The stratified ORR ratio was 0.91(90%CI 0.80-1.04,within the prespecified equivalence margin of 0.75-1.33).Up to May 15^(th),2020,with a median follow-up of 13.6(range 0.8-28.4)months,no notable differences in DCR,median DoR,median PFS,median OS,and 1-year OS rate were observed between the LY01008 and Avastin groups.There were no clinically meaningful differences in safety and immunogenicity across treatment groups.Conclusions:LY01008 demonstrated similarity to Avastin in terms of efficacy and safety in Chinese patients with advanced or recurrent non-squamous NSCLC.LY01008 combined with paclitaxel/carboplatin is expected to become a new treatment option for unresectable,metastatic,LY01008 and Avastin groups.There were no clinically meaningful differences in safety and immunogenicity across treatment groups.Conclusions:LY01008 demonstrated similarity to Avastin in terms of efficacy and safety in Chinese patients with advanced or recurrent non-squamous NSCLC.LY01008 combined with paclitaxel/carboplatin is expected to become a new treatment option for unresectable,metastatic,or recurrent non-squamous NSCLC patients in the first-line setting. | Yuankai Shi Kaijian Lei Yuming Jia Bingqiang Ni Zhiyong He Minghong Bi Xicheng Wang Jianhua Shi Ming Zhou Qian Sun Guolei Wang Dongji Chen Yongqian Shu Lianke Liu Zhongliang Guo Yong Liu Junquan Yang Ke Wang Ke Xiao LinWu Tienan Yi Debin Sun Mafei Kang Tianjiang Ma Yimin Mao Jinsheng Shi Tiegang Tang Yan Wang Puyuan Xing Dongqing Lv Wangjun Liao Zhiguo Luo Bin Wang Xiaohong Wu Xiaoli Zhu Shuhua Han Qisen Guo Rongyu Liu Zhiwei Lu Jianyong Zhang Jian Fang Changlu Hu Yinghua Ji Guolong Liu Hong Lu Dedong Wu Junhong Zhang Shuyang Zhu Zheng Liu Wensheng Qiu Feng Ye Yan Yu Yanqiu Zhao Qinhong Zheng Jun Chen Zhanyu Pan Yiping Zhang Wenjuan Lian Bo Jiang Bo Qiu Guojun Zhang Hua Zhang Yanju Chen Yuan Chen Hongbing Duan Manxiang Li Shengming Liu Lijun Ma Hongming Pan Xia Yuan Xueli Yuan Yulong Zheng Emei Gao Li Zhao Shumin Wang Can Wu | 2021 | Cancer Communications2021，41，9： | 5 |
| 5 | Proteomic Study Identifies Proteins Involved in Brassinosteroid Regulation of Rice Growth显示文摘Brassinosteroids (BRs) are essential hormones for growth and development of plant. In rice, BRs regulate multiple developmental processes and affect many important traits such as height, leaf angle, fertility and seed filling. We identified brassinosteroid-regulated proteins in rice using proteomic approaches and performed functional analysis of some BR-regulated proteins by overexpression experiments. Using two-dimensional difference gel electrophoresis (2-D DIGE) followed by protein identification by mass spectrometry, we compared proteomic differences in the shoots and roots of the BR-insensitive mutant d61-4 and BR-deficient mutant brd1-3. We identified a large number of proteins differentially expressed in the mutants compared with wild type control. These include a glycine-rich RNA-binding protein (OsGRP1) and a DREPP2 protein, which showed reduced levels in the BR mutants. Overexpression of these two proteins partially suppressed the dwarf phenotype of the Arabidopsis BR-insensitive mutant bri1-5. In contrast to the reduced protein level, the RNA level of OsGRP1 was not significantly affected in the BR mutants or by BR treatment, suggesting BR regulation of OsGRP1 at the posttranslational level. This study identifies many BR-regulated proteins and demonstrates that OsGRP1 functions downstream in the BR signal transduction pathway to promote cell expansion. | Fengru Wang Ming-Yi Bai Zhiping Deng Juan A. Oses-Prieto Alma L. Burlingame Tiegang Lu Kang Chong Zhi-Yong Wang | 2010 | Journal of Integrative Plant Biology2010，52，12： | 4 |
| 6 | Paleoproductivity and paleoredox conditions during late Pleistocene accumulation of laminated diatom mats in the tropical West Pacific显示文摘 | Zhifang Xiong Tiegang Li Thomas Algeo Qingyun Nan Bin Zhai Bo Lu | 2012 | Chemical Geology2012，，： | 1 |
| 7 | Activation tagging, an efficient tool for functional analysis of the rice genome显示文摘 | Shuyan Wan Jinxia Wu Zhiguo Zhang Xuehui Sun Yaci Lv Ci Gao Yingda Ning Jun Ma Yupeng Guo Qian Zhang Xia Zheng Caiying Zhang Zhiying Ma Tiegang Lu | 2009 | Plant Molecular Biology (-)2009，，1： | 1 |
| 8 | Effects of Straw and Biochar Returned to the Soil on Soil Physical Properties and pH Value in Cold Rice Region显示文摘[Objectives]In order to explore the feasibility of using straw and biochar returned to the soil to improve soil physical properties and pH value in cold rice regions of China.[Methods]the effects of straw directly returned to the soil and charred straw(biochar)returned to the soil on soil bulk density,porosity,temperature and pH value of cold paddy soil were studied in this paper.[Results]The results showed that compared with conventional production,straw(6 t/ha),a small amount of biochar(2 t/ha)and a large amount of biochar(40 t/ha)returned to the soil reduced paddy soil bulk density at different growth stages by 6.02%-11.86%,2.69%-6.67%and 8.58%-11.32%,respectively,increased total porosity by 7.41%-14.93%,3.19%-8.38%and 9.81%-14.27%,respectively,and increased aeration porosity by 22.28%-192.11%,17.80%-92.11%and 52.44%-157.11%,respectively.Straw and a small amount of biochar returned to the soil had no significant effect on soil temperature and pH value of paddy field,but a large amount of biochar returned to the soil could significantly increase soil temperature by 5.13%-8.79%and pH value by 3.15%-5.96%in the later stage of rice growth.[Conclusions]The straw and biochar returned to the soil could reduce soil bulk density,increase total porosity and aeration porosity,and only a large amount of biochar returned to the soil could significantly increase soil temperature and pH value. | Yuefeng CUI Hongru SHI Aonan GUO Guocai SUN Guiyan WANG Jian WANG Wenjia HUANG Tiegang LU | 2021 | Asian Agricultural Research2021，13，2： | 1 |
| 9 | Structural and functional characteristics of microbiota in oropharynx of sub-healthy children with gastrointestinal heat retention syndrome differentiated by traditional Chinese medicine显示文摘Objective:To explore the structural and functional characteristics of microbiota in oropharynx of subhealthy children with gastrointestinal heat retention syndrome(GHRS)differentiated by traditional Chinese medicine,and screen the biometric operational taxonomic units(OTUs)to assist the clinical diagnosis.Methods:We recruited children according to the“GHRS diagnostic scale”,collected their oropharyngeal swabs,and sequenced the 16 SrDNA V4 region.We described the bacterial structure with alpha-indexes,beta-distances,and relative abundances;moreover,we screened the differential genera/OTUs with Wilcoxon rank-sum test,Metagenome Seq analysis,and linear discriminant analysis effect size(LEf Se)analysis,in which biometric OTUs were selected to construct the receiver operating characteristic curve to verify the diagnostic value.The bacterial function was predicted with Kyoto Encyclopedia of Genes and Genomes pathways according to 16S rDNA gene by using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.Results:The study population was composed of 10 children with GHRS and 10 healthy control children.GHRS children were more likely to overeat(gluttony,P=.033).Alpha-indexes,such as Sobs,abundancebased coverage estimator,Bootstrap,and Qstat,were significantly higher in the GHRS group,while betadistances did not exhibit any significant intergroup differences.There were 9 differently distributed nonpredominant genera between the groups in Wilcoxon rank-sum test,as well as 13 non-predominant genera in Metagenome Seq analysis and 3 non-predominant OTUs in LEfSe analysis.OTU44 and OTU196 were used to construct the receiver operating characteristic curve,and the area under curve was 0.92.Predicted functions showed that pathways related to oxidative phosphorylation and carbon metabolism were enriched in healthy control samples,while the pathway related to renin secretion was remarkably enriched in GHRS samples.Conclusion:Unique oropharyngeal microbial structure and function were identified in GHRS children.OTU44 and OTU196 were specific OTUs,which could be used as biomarkers of GHRS to assist clinical diagnosis. | Jianhua Zhen He Yu Xiaofei Li Fei Dong Zi'an Zheng Xueyan Ma Yuxiang Wan Tiegang Liu Lu Fan Xiaohong Gu | 2022 | Journal of Traditional Chinese Medical Sciences2022，9，3： | 1 |
| 10 | The Differences of Physiological Characteristics and Yield of Northern Japonica Rice in Different Nitrogen Application Patterns显示文摘The research performed analysis on differences of physiological property and yield of Tiejing No. 11 by different nitrogen fertilization patterns. The results indicated that while the nitrogen of basal: tillering: panicle was 6 ∶3∶1, the higher amount of nitrogen improved the chlorophyll content, photosynthetic capacity, effective panicle, grain weight and yield; the medium nitrogen level had advantage under the ratio 4:3:3; nitrogen application at lategrowth stage was suitable for low or medium nitrogen level,which could increase photosynthetic capacity and optimize the yield components and increase yield; under the same ratio for basic tiller and ear fertilizer, the increase of nitrogen could improve total nitrogen uptake and protein content, decrease nitrogen physiological efficiency and milled rice and tasting, but nitrogen recovery and harvest index changes had difference; as application at late growth stage in same nitrogen rate, total nitrogen uptake and protein content increased, nitrogen physiological efficiency and milled rice and amylose content and tasting decreased, nitrogen recovery and harvest index increased under low or medium nitrogen level and decreased under high nitrogen level. Nitrogen application 210 kg/hm^2 and the ratio 4 ∶3 ∶3 of basal:tillering: panicle made the yield increase by 2.8%-11.3%, and this is the optimal mode of nitrogen fertilizer application. | Yuefeng CUI Tiegang LU Guocai SUN Junru WANG Xiaoqiu WU Guiyan WANG Jian WANG Wenjia HUANG | 2016 | Agricultural Science & Technology2016，17，4： | 0 |
| 11 | Development and drought tolerance assay of marker-free transgenic rice with OsAPX2 using biolistic particle-mediated co-transformation显示文摘Abiotic stresses such as drought, salinity, and low temperature cause–losses in rice production worldwide. The emergence of transgenic technology has enabled improvements in the drought resistance of rice plants and helped avert crop damage due to drought stress.Selectable marker genes conferring resistance to antibiotics or herbicides have been widely used to identify genetically modified plants. However, the use of such markers has limited the public acceptance of genetically modified organisms. Marker-free materials (i.e., those containing a single foreign gene) may be more easily accepted by the public and more likely to find common use. In the present study, we created marker-free drought-tolerant transgenic rice plants using particle bombardment. Overall, 842 T_0plants overexpressing the rice ascorbate peroxidase-coding gene OsAPX2 were generated. Eight independentmarker-free lines were identified from T_1 seedlings using the polymerase chain reaction.The molecular characteristics of these lines were examined, including the expression level,copy number, and flanking sequences of OsAPX2, in the T_2 progeny. A simulated drought test using polyethylene glycol and a drought-tolerance test of seedlings confirmed that the marker-free lines carrying OsAPX2 showed significantly improved drought tolerance in seedlings. In the field, the yield of the wild-type plant decreased by 60% under drought conditions compared with normal conditions. However, the transgenic line showed a yield loss of approximately 26%. The results demonstrated that marker-free transgenic lines significantly improved grain yield under drought-stressed conditions. | Dan Feng Yanwei Wang Jinxia Wu Tiegang Lu Zhiguo Zhang | 2017 | The Crop Journal2017，5，4： | 0 |
| 12 | Effects of Nitrogen Fertilization on Growth and Development and Nitrogen Utilization of Japonica Super Rice显示文摘With the super japonica rice Shennong 265 as the tested material, the effects of different nitrogen application amounts and basic tiller fertilizer(BTF)-ear granule fertilizer(EGF) ratios on the yield and nitrogen utilization characteristics of Shennong265 were investigated. The results showed that when the BTF-EGF ratio was 8:2 or 7:3, the yield of Shennong 265 increased with the increased nitrogen application amount; when the BTF-EGF ratio was 6:4, medium nitrogen level was more conducive to improving the yield of Shennong 265; under the condition of same BTF-EGF ratio, with the increased nitrogen application amount, the total nitrogen uptake increased, and the nitrogen physiological efficiency and harvest index decreased. At low and medium nitrogen levels, the higher the proportion of EGF was, the higher the yield, total nitrogen uptake and nitrogen recovery ratio were; at high nitrogen level, the BTF-EGF ratio of 7:3 was more favorable; at the same nitrogen level, the higher the proportion of EGF was, the lower the nitrogen physiological efficiency and harvest index were. Under conditions of nitrogen application level of 255 kg/hm^2 and BTF-EGF ratio of 7:3, the dry matter accumulation in the leaves, stems and spikes of Shennong265 was higher in the late growth period. Higher effective panicle number and grain number per panicle led to higher yield(9581.5 kg/hm^2, 2.4%-20.1% higher than those in the other treatment groups) and higher nitrogen use efficiency. | Yuefeng CUI Guocai SUN Tiegang LU Xiaoqiu WU Helin LUAN | 2017 | Agricultural Science & Technology2017，18，2： | 0 |
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