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| 1 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 2 | In vivo subsurface morphological and functional cellular and subcellular imaging of the gastrointestinal tract with confocal mini-microscopy显示文摘瞄准:为在活体内评估一根最新发达的手持的共焦的探针在啮齿类动物的完全的胃肠道的显微镜的成像。方法:一根新奇僵硬共焦的探针(直径 7 公里) 为 fluorophore 刺激用 488 nm 单身者线激光在人为使用与类似于灵活 endomicroscopy 系统的光特征被设计。轻排放在 505 ~ 750 nm 被检测。看法的地多样地是 475 妈妈 475 妈妈。光片厚度是有 0.7 妈妈的侧面的分辨率的 7 妈妈。在不同深度(到 250 妈妈的表面) 的表面下的连续图象多样地在 1024 点在实时被产生由在轻轻的、稳定的接触把探查放到织物上的 1024 个象素(0.8 frames/s ) 。织物标本为组织病理学说的关联被取样。结果:食管,胃,小并且大肠和中央,肝,胰和胆囊是在在 n = 的高分辨率的视觉 ised 在活体内 48 个鼠标。有共焦的微型显微镜学探查的实时显微镜的成像是容易的完成。不同染色协议(荧光黄,吖啶黄,标记 FITC 的葡聚糖和 L。可食用嗯植物凝血素) 织物的每个加亮的特定的方面,和在活体内成像与常规组织学最优地相关。在活体内血流监视把功能的质量加到词法成像。结论:共焦的显微镜学是在甚至表面下的织物结构的高分辨率允许完全的官方补给的道的可视化的可行在活体内。在这研究评估的新共焦的探查设计与腹腔镜检查兼容并且显著地扩展可能的应用的地到 intra 腹的机关。它允许新在活体内染色和申请选择的立即的测试因此在病人从动物研究允许快速的转移到临床的使用。 | Martin Goetz Beena Memadathil Stefan Biesterfeld Constantin Schneider Sebastian Gregor Peter R Galle Markus F Neurath Ralf Kiesslich | 2007 | World Journal of Gastroenterology2007，13，15： | 6 |
| 3 | Digital templating in total hip arthroplasty:Additional anteroposterior hip view increases the accuracy显示文摘AIM To analyze planning total hip arthroplasty(THA) with an additional anteroposterior hip view may increases the accuracy of preoperative planning in THA.METHODS We conducted prospective digital planning in 100 consecutive patients: 50 of these procedures were planned using pelvic overview only(first group), and the other 50 procedures were planned using pelvic overview plus antero-posterior(a.p.) hip view(second group). The planning and the procedure of each patient were performed exclusively by the senior surgeon. Fifty procedures with retrospective analogues planning were used as the control group(group zero). After the procedure, the planning was compared with the eventually implanted components(cup and stem). For statistic analysis the x^2 test was used for nominal variables and the t test was used for a comparison of continuous variables.RESULTS Preoperative planning with an additional a.p. hip view(second group) significantly increased the exact component correlation when compared to pelvic overview only(first group) for both the acetabular cup and the femoral stem(76% cup and 66% stem vs 54% cup and 32% stem). When considering planning ± 1 size, the accuracy in the second group was 96%(48 of 50 patients) for the cup and 94% for the stem(47 of 50patients). In the analogue control group(group zero), an exact correlation was observed in only 1/3 of the cases.CONCLUSION Digital THA planning performed by the operating surgeon and based on additional a.p. hip view significantly increases the correlation between preoperative planning and eventual implant sizes. | Sophia K Stigler Franz J Müller Sebastian Pfaud Michael Zellner Bernd Füchtmeier | 2017 | World Journal of Orthopedics2017，8，1： | 3 |
| 4 | A novel in vitro as- say for electrophysiological research on human skin fibroblasts: degenerate electrical waves downregulate collagen I expression in keloid fibroblasts显示文摘 | Sebastian A Syed F McGrouther DA | 2011 | Exp Dermatol2011，20，1： | 1 |
| 5 | Sedative and sleep-en hancing properties of linarin, a flavonoid-isolated from Valeriana offi c inalis 显示文摘 | Sebastian F C~istina W Alejandro C | 2004 | Pharmacology Biochemistry and Behavior2004，77，2： | 1 |
| 6 | The influence of resilience on fatigue in cancer patients undergoing radiation therapy (RT)显示文摘 | Bernhard Strauss Christina Brix Sebastian Fischer Karena Leppert Jürgen Füller Bernd Roehrig Christine Schleussner Thomas G. Wendt | 2007 | Journal of Cancer Research and Clinical Oncology2007，，8： | 1 |
| 7 | Delayed umbilical cord clamping for reducing anaemia in low birthweight infants:implications for developing countries显示文摘 | PATRICK F VAN R SEBASTIAN G | 2006 | Annals of Tropical Paediatrics2006，26，： | 1 |
| 8 | Long-term outcomes of low-dose catheter-directed thrombolytic thera- py: a 5-year single-center experience 显示文摘 | Sebastian A J Robinson G J Dyet J F | 2010 | J Vase Interv Radiol2010，21，7： | 1 |
| 9 | Porcine reproductive and respiratory syndrome virus (PRRSV) infection in wild boars 显示文摘 | Gerald R Christina F Sebastian B | 2009 | Veterinary microbiology2009，136，： | 1 |
| 10 | Significance of Morphological Abnormalities Detected by MRI in Patients Undergoing Successful Ablation of Right Ventricular Outflow Tract Tachycardia 显示文摘 | Sebastian G Gerhard K Herbert F | 1997 | Circulation1997，96，3： | 1 |
| 11 | Multiplication and division over the dual basis显示文摘 | SEBASTIAN T J F MOHAMMED B DAVID T | 1996 | IEEE Trans on computers1996，，45： | 1 |
| 12 | 查看详情显示文摘 | Schaefer T Chan W K Sebastian R Schurko R Hruska F E | | 0，，： | 1 |
| 13 | Improving dyna- mic response of power-factor preregulators by using two-input high-efficient postregulators显示文摘 | SEBASTIAN J VILLEGAS P J NUNO F | 1997 | IEEE Transactions on Power Electronic s1997，12，6： | 1 |
| 14 | Performance of a stiff support system in soft clay显示文摘 | RICHARD J F SEBASTIAN B | 2002 | Journal of Geotechnical and Geoenvironmental Engineering2002，128，8： | 1 |
| 15 | Impairments of the protein C system and fibrinolysis in infection-associated stroke 显示文摘 | Richard F Sebastian F Andras G | 1996 | Stroke1996，2711，18： | 1 |
| 16 | Total Cost management of US National Health Care显示文摘 | Dovi Sebastian F | | 0，，11： | 1 |
| 17 | Optimizing the design of single-stage power-factor correctors显示文摘 | Villarejo J Sebastian A J Soto F | 2007 | IEEE Transactions on Industrial Electronics2007，54，3： | 1 |
| 18 | Well - known serial buses for distributed control of backup power plants RS- 485 versus controller area network (CAN) solutions显示文摘 | Castro M Sebastian R Yeves F | 2002 | Industrial Electronics Society2002，3，58： | 1 |
| 19 | Hypoxia in-ducible factor-1 a correlates with VEGF-C expression and lym-phangiogenesis in breast cancer 显示文摘 | Sebastian F? Schoppmannl? Alexandra Fenzl | 2006 | Breast cancer Res Treat2006，99，2： | 1 |
| 20 | Traumatic intervertebral disc lesion – magnetic resonance imaging as a criterion for or against intervertebral fusion显示文摘 | Sebastian Fürderer Klaus Wenda Norbert Thiem Rolf Hachenberger Peer Eysel | 2001 | European Spine Journal2001，，2： | 1 |
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