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| 1 | Proteome analysis of peroxisomes from dark-treated senescent Arabidopsis leaves显示文摘Peroxisomes compartmentalize a dynamic suite of biochemical reactions and play a central role in plant metabolism, such as the degradation of hydrogen peroxide,metabolism of fatty acids, photorespiration, and the biosynthesis of plant hormones. Plant peroxisomes have been traditionally classified into three major subtypes, and in-depth mass spectrometry(MS)-based proteomics has been performed to explore the proteome of the two major subtypes present in green leaves and etiolated seedlings. Here, we carried out a comprehensive proteome analysis of peroxisomes from Arabidopsis leaves given a 48-h dark treatment.Our goal was to determine the proteome of the third major subtype of plant peroxisomes from senescent leaves, and further catalog the plant peroxisomal proteome. We identifieda total of 111 peroxisomal proteins and verified the peroxisomal localization for six new proteins with potential roles in fatty acid metabolism and stress response by in vivo targeting analysis. Metabolic pathways compartmentalized in the three major subtypes of peroxisomes were also compared, which revealed a higher number of proteins involved in the detoxification of reactive oxygen species in peroxisomes from senescent leaves. Our study takes an important step towards mapping the ful function of plant peroxisomes. | Ronghui Pan Sigrun Reumann Piotr Lisik Stefanie Tietz Laura J.Olsen Jianping Hu | 2018 | Journal of Integrative Plant Biology2018，60，11： | 5 |
| 2 | Caffeic acid phenethyl ester protects against oxidative stress and dampens inflammation via heme oxygenase 1显示文摘Periodontal disease is associated with chronic oxidative stress and inflammation. Caffeic acid phenethyl ester(CAPE), which is a potent inducer of heme oxygenase 1(HO1), is a central active component of propolis, and the application of propolis improves periodontal status in diabetic patients. Here, primary murine macrophages were exposed to CAPE. Target gene expression was assessed by whole-genome microarray, RT-PCR and Western blotting. The antioxidative and anti-inflammatory activities of CAPE were examined by exposure of the cells to hydrogen peroxide, saliva and periodontal pathogens. The involvement of HO1 was investigated with the HO1 inhibitor tin protoporphyrin(SnPP) and knockout mice for Nrf2, which is a transcription factor for detoxifying enzymes. CAPE increased HO1 and other heat shock proteins in murine macrophages. A p38 MAPK inhibitor and Nrf2 knockout attenuated CAPE-induced HO1 expression in macrophages. CAPE exerted strong antioxidative activity. Additionally, CAPE reduced the inflammatory response to saliva and periodontal pathogens. Blocking HO1 decreased the antioxidative activity and attenuated the anti-inflammatory activity of CAPE. In conclusion, CAPE exerted its antioxidative effects through the Nrf2-mediated HO1 pathway and its anti-inflammatory effects through NF-κB inhibition. However, preclinical models evaluating the use of CAPE in periodontal inflammation are necessary in future studies. | Alexandra St?hli Ceeneena Ubaidha Maheen Franz Josef Strauss Sigrun Eick Anton Sculean Reinhard Gruber | 2019 | International Journal of Oral Science2019，11，1： | 4 |
| 3 | The anti-microbial peptide LL-37 modulates immune responses in the palatine tonsils where it is exclusively expressed by neutrophils and a subset of dendritic cells显示文摘 | Sigrun L. Sigurdardottir Ragna H. Thorleifsdottir Andrew M. Guzman Gudmundur H. Gudmundsson Helgi Valdimarsson Andrew Johnston | 2011 | Clinical Immunology2011，，2： | 2 |
| 4 | Shifts in physiological capabilities of the micro biota during the decomposition of leaf litter in a black alder forest 显示文摘 | Oliver D Sigrun B Petra R | 2001 | Soil Biology Biochemistry2001，33，78： | 1 |
| 5 | Asymptotic Behavior of Solutions of Dynamic Equations显示文摘 | Sigrun Bodine Martin Bohner Donald Lutz | 2004 | Journal of Mathematical Sciences2004，，4： | 1 |
| 6 | Use of Cohesive Features in ESL Students’ E-mail and Word-Processed Texts: A Comparative Study显示文摘 | Sigrun Biesenbach-Lucas Christine Meloni Donald Weasenforth | 2000 | Computer Assisted Language Learning2000，，3： | 1 |
| 7 | Fish oil supplementation in the parenteral nutrition of critically ill medical patients: a randomized controlled trial显示文摘 | SIGRUN F CHRISTIAN L JENNY K | 2008 | Intensive Care Med2008，34，8： | 1 |
| 8 | Aerobic moving bed biofilm reactor treating thermomechanical pulping white water under thermophilic conditions显示文摘 | Sigrun J J Jukka A R Hallvard O | 2002 | Water Research2002，36，： | 1 |
| 9 | Induction of specific T cell immunity in patients with prostate cancer by vaccination with PSA146–154 peptide显示文摘 | Supriya Perambakam Sigrun Hallmeyer Samarth Reddy Nadim Mahmud Linda Bressler Phillip DeChristopher Delores Mahmud Rafael Nunez Jeffrey A. Sosman David J. Peace | 2006 | Cancer Immunology Immunotherapy2006，，9： | 1 |
| 10 | The influence of freeze-thaw cycles and soil moisture on aggregate stability of three soils in Norway显示文摘 | SIGRUN H K LILLIAN O | | 0，，： | 1 |
| 11 | A unified treatment of nonclassical nonlinear effects in the propagation of ultrasound in heterogeneous media显示文摘 | Pier Paolo Delsanto Sigrun Hirsekom | 2004 | Ultrasonics2004，42，1： | 1 |
| 12 | New guidelines for fluorophore application in peroxisome targeting analyses in transient plant expression systems显示文摘Peroxisome research has been revolutionized by proteome studies combined with in vivo subcellular targeting analyses. Yellow and cyan fluorescent protein(YFP and CFP) are the classical fluorophores of plant peroxisome research. In the new transient expression system of Arabidopsis seedlings co-cultivated with Agrobacterium we detected the YFP fusion of one candidate protein in peroxisomes, but only upon co-transformation with the peroxisome marker, CFP-PTS1. The data suggested that the YFP fusion was directed to peroxisomes due to its weak heterodimerization ability with CFP-PTS1,allowing piggy-back import into peroxisomes. Indeed, if co-expressed with monomeric Cerulean-PTS1(mCer-PTS1),the YFP fusion was no longer matrix localized. We systematically investigated the occurrence and extent of dimerization-based piggy-back import for different fluorophore combinations in five major transient plant expression systems. In Arabidopsis seedlings and tobacco leaves both untagged YFP and monomeric Venus were imported into peroxisomes if co-expressed with CFP-PTS1 but not with mCer-PTS1. By contrast, piggy-back import of cytosolic proteins was not observed in Arabidopsis and tobacco protoplasts or in onion epidermal cells for any fluorophore combination at any time point. Based on these important results we formulate new guidelines for fluorophore usage and experimental design to guarantee reliable identification of novel plant peroxisomal proteins. | Christian Falter Nguyen Binh Anh Thu Saugat Pokhrel Sigrun Reumann | 2019 | Journal of Integrative Plant Biology2019，61，7： | 1 |
| 13 | Elastici- ty mapping of precipitates in polycrystalline materials using atomic force acoustic microscopy 显示文摘 | Kumar Anish Rabe Ute Hirsekorn Sigrun | 2008 | Applied Physics Let-ten2008，92，18： | 1 |
| 14 | Treatment of thermomechanical pulping (TMP) whitewater in thermophilie (55 ℃) anaerobic-aerobic moving bed biofilm reactors 显示文摘 | Jahren Sigrun J Odegaard HaIlvard | 1999 | Water Sci Technol1999，40，8： | 1 |
| 15 | Induction of specific T cell immunity in patients with prostate cancer by vaccination with PSA146–154 peptide显示文摘 | Supriya Perambakam Sigrun Hallmeyer Samarth Reddy Nadim Mahmud Linda Bressler Phillip DeChristopher Delores Mahmud Rafael Nunez Jeffrey A. Sosman David J. Peace | 2006 | Cancer Immunology Immunotherapy2006，，9： | 1 |
| 16 | The influence of freeze-thaw cycles and soil moisture on aggregate stability of three soils in Norway显示文摘 | Sigrun H K Lillian (O) | | 0，，： | 1 |
| 17 | Sympathetic activation in broadly defined generalized anxiety disorder显示文摘 | Walton T. Roth Sigrun Doberenz Anja Dietel Ansgar Conrad Anett Mueller Eileen Wollburg Alicia E. Meuret C. Barr Taylor Sunyoung Kim | 2007 | Journal of Psychiatric Research2007，，3： | 1 |
| 18 | Impact of holmium: YAG and neodymium: YAG lasers on the efficacy of DNA delivery in transitional cell carcinoma显示文摘 | Thomas Knoll Lutz Trojan Sigrun Langbein | 2004 | Lasers in Medical Science2004，19，： | 1 |
| 19 | Profils ins Hochschulmanagement -Pl(a)doyer für die Schaffung von hauptamtlichen Karrierewegen für Hochschul-und Fakult (a) tsleitungen显示文摘 | Ziegele Frank | 2006 | Hochschulmanagement2006，，1： | 1 |
| 20 | A double-blind placebo-controlled trial to study therapeutic effects of probiotic Escherichia coli Nissle 1917 in subgroups of patients with irritable bowel syndrome显示文摘 | Wolfgang Kruis Sigrun Chrubasik Stephan Boehm Christiane Stange Juergen Schulze | 2012 | International Journal of Colorectal Disease2012，，4： | 1 |
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