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| 1 | DNA-informed breeding of rosaceous crops: promises, progress and prospects显示文摘Crops of the Rosaceae family provide valuable contributions to rural economies and human health and enjoyment.Sustained solutions to production challenges and market demands can be met with genetically improved new cultivars.Traditional rosaceous crop breeding is expensive and time-consuming and would benefit from improvements in efficiency and accuracy.Use of DNA information is becoming conventional in rosaceous crop breeding,contributing to many decisions and operations,but only after past decades of solved challenges and generation of sufficient resources.Successes in deployment of DNA-based knowledge and tools have arisen when the‘chasm’between genomics discoveries and practical application is bridged systematically.Key steps are establishing breeder desire for use of DNA information,adapting tools to local breeding utility,identifying efficient application schemes,accessing effective services in DNA-based diagnostics and gaining experience in integrating DNA information into breeding operations and decisions.DNA-informed germplasm characterization for revealing identity and relatedness has benefitted many programs and provides a compelling entry point to reaping benefits of genomics research.DNA-informed germplasm evaluation for predicting trait performance has enabled effective reallocation of breeding resources when applied in pioneering programs.DNA-based diagnostics is now expanding from specific loci to genome-wide considerations.Realizing the full potential of this expansion will require improved accuracy of predictions,multi-trait DNA profiling capabilities,streamlined breeding information management systems,strategies that overcome plant-based features that limit breeding progress and widespread training of current and future breeding personnel and allied scientists. | Cameron P Peace | 2017 | Horticulture Research2017，4，1： | 11 |
| 2 | Elucidation of the ‘Honeycrisp’ pedigree through haplotype analysis with a multi-family integrated SNP linkage map and a large apple (Malus×domestica) pedigree-connected SNP data set显示文摘The apple(Malus×domestica)cultivar Honeycrisp has become important economically and as a breeding parent.An earlier study with SSR markers indicated the original recorded pedigree of‘Honeycrisp’was incorrect and‘Keepsake’was identified as one putative parent,the other being unknown.The objective of this study was to verify‘Keepsake’as a parent and identify and genetically describe the unknown parent and its grandparents.A multi-family based dense and high-quality integrated SNP map was created using the apple 8 K Illumina Infinium SNP array.This map was used alongside a large pedigree-connected data set from the RosBREED project to build extended SNP haplotypes and to identify pedigree relationships.‘Keepsake’was verified as one parent of‘Honeycrisp’and‘Duchess of Oldenburg’and‘Golden Delicious’were identified as grandparents through the unknown parent.Following this finding,siblings of‘Honeycrisp’were identified using the SNP data.Breeding records from several of these siblings suggested that the previously unreported parent is a University of Minnesota selection,MN1627.This selection is no longer available,but now is genetically described through imputed SNP haplotypes.We also present the mosaic grandparental composition of‘Honeycrisp’for each of its 17 chromosome pairs.This new pedigree and genetic information will be useful in future pedigree-based genetic studies to connect‘Honeycrisp’with other cultivars used widely in apple breeding programs.The created SNP linkage map will benefit future research using the data from the Illumina apple 8 and 20 K and Affymetrix 480 K SNP arrays. | Nicholas P Howard Eric van de Weg David S Bedford Cameron P Peace Stijn Vanderzande Matthew D Clark Soon Li Teh Lichun Cai James J Luby | 2017 | Horticulture Research2017，4，1： | 9 |
| 3 | Prunus genetics and applications after de novo genome sequencing:achievements and prospects显示文摘Prior to the availability of whole-genome sequences,our understanding of the structural and functional aspects of Prunus tree genomes was limited mostly to molecular genetic mapping of important traits and development of EST resources.With public release of the peach genome and others that followed,significant advances in our knowledge of Prunus genomes and the genetic underpinnings of important traits ensued.In this review,we highlight key achievements in Prunus genetics and breeding driven by the availability of these whole-genome sequences.Within the structural and evolutionary contexts,we summarize:(1)the current status of Prunus whole-genome sequences;(2)preliminary and ongoing work on the sequence structure and diversity of the genomes;(3)the analyses of Prunus genome evolution driven by natural and man-made selection;and(4)provide insight into haploblocking genomes as a means to define genome-scale patterns of evolution that can be leveraged for trait selection in pedigree-based Prunus tree breeding programs worldwide.Functionally,we summarize recent and ongoing work that leverages whole-genome sequences to identify and characterize genes controlling 22 agronomically important Prunus traits.These include phenology,fruit quality,allergens,disease resistance,tree architecture,and self-incompatibility.Translationally,we explore the application of sequence-based marker-assisted breeding technologies and other sequence-guided biotechnological approaches for Prunus crop improvement.Finally,we present the current status of publically available Prunus genomics and genetics data housed mainly in the Genome Database for Rosaceae(GDR)and its updated functionalities for future bioinformatics-based Prunus genetics and genomics inquiry. | Maria JoséAranzana Véronique Decroocq Elisabeth Dirlewanger Iban Eduardo Zhong Shan Gao Ksenija Gasic Amy Iezzoni Sook Jung Cameron Peace Humberto Prieto Ryutaro Tao Ignazio Verde Albert G.Abbott Pere Arús | 2019 | Horticulture Research2019，6，1： | 8 |
| 4 | 海藻糖快速检测方法的建立与初步应用显示文摘海藻糖是生物体合成的、具有抗逆保护作用的天然二糖,在食品加工与贮藏、微生物发酵过程具有重要作用。该文采用前期获得的专一性海藻糖酶,建立了一种定量测定海藻糖的快速方法。该方法对海藻糖的检测限为0.019 mg/m L,定量限为0.1 mg/m L;海藻糖的线性检测范围为0.1 mg/m L^150.0 mg/m L,回收率为98.80%~101.33%。该方法操作简便快捷、特异性高、重复性好,在菌种改良与食品原料营养筛查等海藻糖快速定量检测中具有良好的应用前景。 | 苗佳 王彩喆 牛丹丹 Nokuthula Peace Mchunu 田康明 Suren Singh Kugenthiren Permaul 王正祥 | 2019 | 食品与发酵工业2019，45，23： | 5 |
| 5 | Is in vivo measurement of size of polyps during colonoscopy accurate?显示文摘 | Narasimh Gopalswamy Vishwanath N. Shenoy Umesh Choudhry Ronald J. Markert Nancy Peace Manoop S. Bhutani Christopher J. Barde | 1997 | Gastrointestinal Endoscopy1997，，6： | 3 |
| 6 | 疏绵状嗜热丝孢菌外切β-葡聚糖酶的基因克隆与酶学特征显示文摘【目的】疏绵状嗜热丝孢菌是一种嗜热丝状真菌,具有合成与分泌多种耐热酶的能力,从中找寻酶活高、耐热性能优良的β-葡聚糖酶。【方法】对疏绵状嗜热丝孢菌其中一个外切β-葡聚糖酶的编码基因gln B进行克隆,并在毕赤酵母GS115中表达。【结果】在摇瓶水平上重组菌的产酶活为11.5 U/m L,重组酶在SDS-PAGE中的大小约为48 k D。重组Gln B最适作用温度为65°C,最适作用p H为5.0;在低于50°C或p H 3.0-10.0之间具有良好稳定性。重组酶水解海带三糖,首先生成单糖和二糖,延长酶作用时间,可以进一步将其中的二糖部分水解为单糖。【结论】疏绵状嗜热丝孢菌来源的重组酶Gln B为外切β-葡聚糖酶,具有较好的耐热性能和p H稳定性。 | 王瑞杰 Nokuthula Peace Mchunu 牛丹丹 Kugenthiren Permaul Suren Singh 刘晓光 路福平 | 2016 | 微生物学通报2016，43，2： | 3 |
| 7 | RosBREED:bridging the chasm between discovery and application to enable DNA-informed breeding in rosaceous crops显示文摘The Rosaceae crop family(including almond,apple,apricot,blackberry,peach,pear,plum,raspberry,rose,strawberry,sweet cherry,and sour cherry)provides vital contributions to human well-being and is economically significant across the U.S.In 2003,industry stakeholder initiatives prioritized the utilization of genomics,genetics,and breeding to develop new cultivars exhibiting both disease resistance and superior horticultural quality.However,rosaceous crop breeders lacked certain knowledge and tools to fully implement DNA-informed breeding—a“chasm”existed between existing genomics and genetic information and the application of this knowledge in breeding.The RosBREED project(“Ros”signifying a Rosaceae genomics,genetics,and breeding community initiative,and“BREED”,indicating the core focus on breeding programs),addressed this challenge through a comprehensive and coordinated 10-year effort funded by the USDA-NIFA Specialty Crop Research Initiative.RosBREED was designed to enable the routine application of modern genomics and genetics technologies in U.S.rosaceous crop breeding programs,thereby enhancing their efficiency and effectiveness in delivering cultivars with producer-required disease resistances and market-essential horticultural quality.This review presents a synopsis of the approach,deliverables,and impacts of RosBREED,highlighting synergistic global collaborations and future needs.Enabling technologies and tools developed are described,including genome-wide scanning platforms and DNA diagnostic tests.Examples of DNA-informed breeding use by project participants are presented for all breeding stages,including pre-breeding for disease resistance,parental and seedling selection,and elite selection advancement.The chasm is now bridged,accelerating rosaceous crop genetic improvement. | Amy F.Iezzoni Jim McFerson James Luby Ksenija Gasic Vance Whitaker Nahla Bassil Chengyan Yue Karina Gallardo Vicki McCracken Michael Coe Craig Hardner Jason D.Zurn Stan Hokanson Eric van de Weg Sook Jung Dorrie Main Cassia da Silva Linge Stijn Vanderzande Thomas M.Davis Lise L.Mahoney Chad Finn Cameron Peace | 2020 | Horticulture Research2020，7，1： | 2 |
| 8 | The Hard Work of Being a Soft Manager显示文摘 | Peace William H | 2001 | Harvard Business Review2001，79，11： | 1 |
| 9 | Microsurgical anatomy of the superior cerebellar artery 显示文摘 | Hardy DG Peace DA Rhoton AL Jr | 1980 | Neurosurgery1980，6，1： | 1 |
| 10 | 高转苷活性乳糖酶编码基因的克隆与酶学特征研究显示文摘运用鸟枪克隆术,从环状芽胞杆菌B2301基因组中克隆出乳糖酶编码基因,其完整读框大小为5133 bp,编码1710个氨基酸残基,不含典型细菌信号肽序列,与已有报道的β-半乳糖苷酶的最高一致性为93.6%。初步表达和纯化了此乳糖酶,重组乳糖酶在50℃(ONPG)/60℃(乳糖)和pH 6.0~6.5下表现出最高催化活性,Zn 2+、Fe 2+、Cu 2+、EDTA和SDS对重组酶表现出不同程度的抑制作用;该酶在50和55℃下催化合成低聚半乳糖的V max分别为2.21和2.47 g/(L·h),K m分别为10.46和14.37 g/L。所获得的乳糖酶具有以乳糖为底物酶法合成低聚半乳糖的优良应用属性,可为后续针对此酶的高效表达与工业化应用奠定基础。 | 田康明 赵继华 牛丹丹 Nokuthula Peace MCHUNU 王彩喆 王正祥 | 2020 | 食品与发酵工业2020，46，15： | 1 |
| 11 | Microsurgieal anatomy of the deep venous system of the brain显示文摘 | ONO M RHOTON A L JR PEACE D | 1984 | Neurosurgery1984，15，： | 1 |
| 12 | Identification of three related hu- man GRO genes encoding cytokine functions 显示文摘 | Haskill S Peace A Morris J | 1990 | Proc Natl Acad Sci1990，87，： | 1 |
| 13 | Distal posterior inferior cerebellar artery aneurysms:clinical features and management显示文摘 | Lewis SB Chang DJ Peace DA | 2002 | J Neurosurg2002，97，4： | 1 |
| 14 | Angiotensin converting-enzyme inhibition in stable coronary artery disease显示文摘 | The PEACE Trial Investigators | 2004 | N Engl J Med2004，351，： | 1 |
| 15 | Microsurgery of the third ventricle:Part 2-Operative approaches显示文摘 | Rhoton AL Jr Yamamoto I Peace DA | 1981 | Neurosurgery1981，8，4： | 1 |
| 16 | Stochastic combination of load effects 显示文摘 | PEACE H T WEN Y K | 1984 | Journal of Structural Engineering1984，110，7： | 1 |
| 17 | Met - related receptor tyrosine kinase Ron in tumor growth and metastasis 显示文摘 | Wagh PK Peace BE Waltz SE | 2008 | Adv Cancer Res2008，100，： | 1 |
| 18 | Comparable kinetics of myeloablation between fludarabine/full-dose busulfan and fludarabine/melphalan conditioning regimens in allogeneic peripheral blood stem cell transplantation 显示文摘 | Chunduri S Dobogai LC Peace D | 2006 | Bone Marrow Transplantation2006，38，： | 1 |
| 19 | The coming carbon market and its impact on the Amer/can economy 显示文摘 | PEACE J JULIANI T | 2009 | Policy and Society2009，，27： | 1 |
| 20 | Lichens and hryophyte communities of planted and semi-natural forests in Britaint the influence of site type,stand structure and dead wood显示文摘 | Humphrey J W Davey S Peace A J | 2002 | Biological Conservation2002，，107： | 1 |
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