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| 1 | Mesenchymal stem cell-derived exosomes promote neurogenesis and cognitive function recovery in a mouse model of Alzheimer’s disease显示文摘Studies have shown that mesenchymal stem cell-derived exosomes can enhance neural plasticity and improve cognitive impairment.The purpose of this study was to investigate the effects of mesenchymal stem cell-derived exosomes on neurogenesis and cognitive capacity in a mouse model of Alzheimer’s disease.Alzheimer’s disease mouse models were established by injection of beta amyloid 1?42 aggregates into dentate gyrus bilaterally.Morris water maze and novel object recognition tests were performed to evaluate mouse cognitive deficits at 14 and 28 days after administration.Afterwards,neurogenesis in the subventricular zone was determined by immunofluorescence using doublecortin and PSA-NCAM antibodies.Results showed that mesenchymal stem cells-derived exosomes stimulated neurogenesis in the subventricular zone and alleviated beta amyloid 1?42-induced cognitive impairment,and these effects are similar to those shown in the mesenchymal stem cells.These findings provide evidence to validate the possibility of developing cell-free therapeutic strategies for Alzheimer’s disease.All procedures and experiments were approved by Institutional Animal Care and Use Committee(CICUAL)(approval No.CICUAL 2016-011)on April 25,2016. | Edwin E. Reza-Zaldivar Mercedes A. Hernández-Sapiéns Yanet K. Gutiérrez-Mercado Sergio Sandoval-ávila Ulises Gomez-Pinedo Ana L. Márquez-Aguirre Estefanía Vázquez-Méndez Eduardo Padilla-Camberos Alejandro A. Canales-Aguirre | 2019 | Neural Regeneration Research2019，14，9： | 19 |
| 2 | Interplay between post-translational cyclooxygenase-2 modifications and the metabolic and proteomic profile in a colorectal cancer cohort显示文摘BACKGROUND Colorectal cancer(CRC) is the second most common cause of cancer death worldwide. It is broadly described that cyclooxygenase-2(COX-2) is mainly overexpressed in CRC but less is known regarding post-translational modifications of this enzyme that may regulate its activity, intracellular localization and stability. Since metabolic and proteomic profile analysis is essential for cancer prognosis and diagnosis, our hypothesis is that the analysis of correlations between these specific parameters and COX-2 state in tumors of a high number of CRC patients could be useful for the understanding of the basis of this cancer in humans.AIM To analyze COX-2 regulation in colorectal cancer and to perform a detailed analysis of their metabolic and proteomic profile.METHODS Biopsies from both healthy and pathological colorectal tissues were taken under informed consent from patients during standard colonoscopy procedure in the University Hospital of Bellvitge(Barcelona, Spain) and Germans Trias i Pujol University Hospital(Campus Can Ruti)(Barcelona, Spain). Western blot analysis was used to determine COX-2 levels. Deglycosylation assays were performed in both cells and tumor samples incubating each sample with peptide N-glycosidase F(PNGase F). Prostaglandin E2(PGE2) levels were determined using a specific ELISA. 1 H high resolution magic angle spinning(HRMAS) analysis was performed using a Bruker AVIII 500 MHz spectrometer and proteomic analysis was performed in a nano-liquid chromatography-tandem mass spectrometer(nano LC-MS/MS) using a QExactive HF orbitrap MS.RESULTS Our data show that COX-2 has a differential expression profile in tumor tissue of CRC patients vs the adjacent non-tumor area, which correspond to a glycosylated and less active state of the protein. This fact was associated to a lesser PGE2 production in tumors. These results were corroborated in vitro performing deglycosylation assays in HT29 cell line where COX-2 protein profile was modified after PNGase F incubation, showing higher PGE2 levels. Moreover,HRMAS analysis indicated that tumor tissue has altered metabolic features vs non-tumor counterparts, presenting increased levels of certain metabolites such as taurine and phosphocholine and lower levels of lactate. In proteomic experiments, we detected an enlarged number of proteins in tumors that are mainly implicated in basic biological functions like mitochondrial activity,DNA/RNA processing, vesicular trafficking, metabolism, cytoskeleton and splicing.CONCLUSION In our colorectal cancer cohort, tumor tissue presents a differential COX-2 expression pattern with lower enzymatic activity that can be related to an altered metabolic and proteomic profile. | Patricia Prieto Rafael I Jaén Daniel Calle María Gómez-Serrano Estefanía Nú?ez María Fernández-Velasco Paloma Martín-Sanz Sergio Alonso Jesús Vázquez Sebastián Cerdán Miguel ángel Peinado Lisardo Boscá | 2019 | World Journal of Gastroenterology2019，25，4： | 4 |
| 3 | Minimum probability of error for asynchronous gaussian multiple-access channels显示文摘 | SERGIO V | 1986 | IEEE Trans Inform Theory1986，32，1： | 2 |
| 4 | Diabetic cardiomyopathy: mechanisms and therapeutic targets显示文摘 | Pavan K Battiprolu Thomas G Gillette Zhao V Wang Sergio Lavandero Joseph A Hill | 2010 | Drug Discovery Today: Disease Mechanisms2010，，2： | 2 |
| 5 | Deregulation of microRNA-503 Contributes to Diabetes Mellitus–Induced Impairment of Endothelial Function and Reparative Angiogenesis After Limb Ischemia显示文摘 | Andrea Caporali Marco Meloni Christine V?llenkle Desiree Bonci Graciela B. Sala-Newby Roberta Addis Gaia Spinetti Sergio Losa Rachel Masson Andrew H. Baker Reuven Agami Carlos le Sage Gianluigi Condorelli Paolo Madeddu Fabio Martelli Costanza Emanueli | 2011 | Circulation2011，，3： | 2 |
| 6 | Relief of pruritus and decrease in plasma histamine concentrations during erythropoietin therapy in patients with uremia显示文摘 | Sergio D Emanuela C Danilo V et a1 | 1992 | N Eng J Med1992，326，1： | 1 |
| 7 | Numerical sim- ulation of gas-liquid dynamics in cylindrical bubble col- umn reactors显示文摘 | Sanyal J Sergio V Shantanu R | 1999 | Chem Eng Sci1999，54，： | 1 |
| 8 | Transesterifica- tion of Vegetable Oils:Simulating the Replacement of Batch Reactors with Continuous Reactors 显示文摘 | Felipe A S F Jose A V Sergio P R | 2010 | Bioresource Technology2010，101，： | 1 |
| 9 | Method development for the simultaneous determination of methylmercury and inorganic mercury in seafood显示文摘 | Ariane V Z Sergio C Carmen I P | 2014 | Food Control2014，46，1： | 1 |
| 10 | Benign convulsions associated with mild gastroenteritis:a multicenter clinical study显示文摘 | Alberto V Giulian N Sergio A | | 0，，2： | 1 |
| 11 | Comparison of the usefulness of cardiac resynchronization therapy in three age-groups (<65, 65-74 and ≥75 Years) (from the InSync/InSync ICD Italian Registry)显示文摘 | Stefano F Sergio V Giuseppe B | 2011 | Am J Cardiol2011，107，10： | 1 |
| 12 | A model-based direct power control for three-phase power converters显示文摘 | SERGIO V Juan A S Juan M C | | 0，，04： | 1 |
| 13 | Predictive control strategy for DC/AC converters based on direct power control显示文摘 | Sergio A L Miguel A R V Estanis O | 2007 | IEEE Trans on Industrial Electronics2007，54，3： | 1 |
| 14 | Relief of pruritus anti decrease in plasma histamine concentrations during erythropoietin therapy in patients with uremia显示文摘 | Sergio D Emanuela C Danilo V | 1992 | N Engl J Med1992，326，： | 1 |
| 15 | Relief of pruritus and decrease in plasma histamine concentrations during erythropoietin therapy in patients with uremia显示文摘 | Sergio D Emanuela C Danilo V | 1992 | N Engl J Med1992，326，： | 1 |
| 16 | Relief of pruritus and decrease inplasma histamine concentrations during erythropoietin therapy in patientswith uremia显示文摘 | Sergio D Emanuela C Danilo V | 1992 | JMed1992，326，： | 1 |
| 17 | Relief of Pruritu and Decrease in Plasma Histamine Comcertrations during Ery 2 Thropoietin Therapy in Patients with Uremia显示文摘 | Sergio D Emanuela C Danilo V | 1992 | N Engl J Med1992，326，15： | 1 |
| 18 | Optimi-zation of the hydrolytic-acidogenic anaerobic digestion stage(55℃)of sewage sludge:Influence of pH and solid content显示文摘 | Sergio Ponsáa Ivet Ferrerb Felícitas Vázquezc | 2008 | WaterResearch2008，42，14： | 1 |
| 19 | Cardiovascular risk as- sessment of pulmonary embolism with the GRACE Risk Score 显示文摘 | Luis V P Rui C P Sergio N B | 2013 | Am J Cardiol2013，111，2： | 1 |
| 20 | QTL analysis of plant development and fruit traits in pepper and performance of selective phenotyping显示文摘 | Lorenzo Barchi Véronique Lefebvre Anne-Marie Sage-Palloix Sergio Lanteri Alain Palloix | 2009 | Theoretical and Applied Genetics2009，，6： | 1 |
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