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| 1 | Antineoplastic effects of deoxyelephantopin,a sesquiterpene lactone from Elephantopusscaber, on lung adenocarcinoma (A549) cells显示文摘OBJECTIVE: Deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, showed inhibition of the growth of various tumor cells in vitro. In the present study, we investigated the cytotoxicity and apoptosis-inducing capacity of deoxyelephantopin on lung adenocarcinoma (A549) cells. METHODS: The cytotoxic effect of deoxyelephantopin on A549 cells and normal lymphocytes was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and 50% inhibitory concentration (IC50) value was determined. The self-renewal and proliferating potential of A549 cells after treatment with deoxyelephantopin were examined by colony formation assay. Cellular morphology of deoxyelephantopin-treated cells was observed using phasecontrast microscopy. The induction of apoptosis was evaluated using acridine orange and ethidium bromide staining, Hoechst 33342 staining, terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labeling (TUNEL) assay, DNA fragmentation analysis and Annexin V-fluorescein isothiocyanate staining by flow cytometry. Activation of caspases was detected using fluorogenic substrate specific to caspases 2, 3, 8 and 9 and flow cytometric analysis. The total cellular DNA content and expression of cleaved poly (ADP-ribose) polymerase was also analyzed. RESULTS: Deoxyelephantopin exhibited cytotoxicity to A549 cells (IC50=12.287 μg/mL), however, there was no toxicity towards normal human lymphocytes. Deoxyelephantopin suppressed the colony-forming ability of A549 cells in a dose-dependent manner. Acridine orange, ethidium bromide and Hoechst 33342 staining showed cell shrinkage, chromosomal condensation and nuclear fragmentation, indicating induction of apoptosis. Deoxyelephantopin increased apoptosis of A549 cells, as evidenced by more TUNEL-positive cells. DNA fragmentation and Annexin V staining revealed late-stage apoptotic cell population. Deoxyelephantopin inhibited A549 cell growth by cell cycle arrest at G2/M phase and induced apoptosis through both extrinsic and intrinsic pathways. CONCLUSION: These results suggest that deoxyelephantopin has great potential as a new chemotherapeutic agent to be developed further for the treatment of lung cancer. | Farha A. Kabeer Geetha B. Sreedevi Mangalam S. Nair Dhanya S. Rajalekshmi LathaP. Gopalakrishnan Sujathan Kunjuraman Remani Prathapan | 2013 | Journal of Integrative Medicine2013，11，4： | 4 |
| 2 | 采用遗传算法和基于断裂准则的破坏机理进行复合板优化设计显示文摘基于破坏机制、最大应力和Tsai-Wu破坏准则,提出复合层压板的最小质量设计。其目的是论证复合材料设计中最近提出的FMB破坏准则的有效性。破坏准则认为不同的破坏机制,如,纤维破坏、模具破裂、纤维压缩破坏以及模具压碎,与不同的荷载条件相关。将遗传算法用于优化研究中,当层压板受到压-压荷载时,与破坏机制和最大破坏应力准则相比,在破坏包络图的第三象限,Tsai-Wu破坏准则预测超过预期层压板质量达到86%。破坏机制以及最大破坏应力标准给出了可比较的质量标准。破坏准则可以考虑用于复合结构的强度设计。 | G. Narayana Naik S. Gopalakrishnan Ranjan Ganguli | 2008 | 钢结构2008，23，9： | 4 |
| 3 | Synthesis, characterization and corrosion protection property of terpolymers derived from poly(MAn- co -MMA) containing benzimidazole derivative as pendant group显示文摘 | S. Anne Pauline S. Sahila C. Gopalakrishnan S. Nanjundan N. Rajendran | 2011 | Progress in Organic Coatings2011，，3： | 1 |
| 4 | Periodontal diseases in Europe显示文摘 | Aubrey Sheiham Gopalakrishnan S. Netuveli | 2002 | Periodontology 20002002，，1： | 1 |
| 5 | Effect of Feronia limonia on mosquito larvae显示文摘 | A.Abdul Rahuman Geetha Gopalakrishnan B.Saleem Ghouse S. Arumugam B. Himalayan | 2000 | Fitoterapia2000，，5： | 1 |
| 6 | Molecular identification and phylogenetic relationships of seven Indian Sciaenids (Pisces: Perciformes, Sciaenidae) based on 16S rRNA and cytochrome c oxidase subunit I mitochondrial genes显示文摘 | Wazir S. Lakra M. Goswami A. Gopalakrishnan | 2009 | Molecular Biology Reports2009，，5： | 1 |
| 7 | Nature inspired optimization techniques for the design optimization of laminated composite structures using failure criteria显示文摘 | G. Narayana Naik S.N. Omkar Dheevatsa Mudigere S. Gopalakrishnan | 2010 | Expert Systems With Applications2010，，3： | 1 |
| 8 | Artificial Bee Colony (ABC) for multi-objective design optimization of composite structures显示文摘 | S.N. Omkar J. Senthilnath Rahul Khandelwal G. Narayana Naik S. Gopalakrishnan | 2009 | Applied Soft Computing Journal2009，，1： | 1 |
| 9 | Jugular phlebectasia: theory of pathogenesis and review of literature显示文摘 | Vinidh Paleri S. Gopalakrishnan | 2001 | International Journal of Pediatric Otorhinolaryngology2001，，2： | 1 |
| 10 | Incorporating delayed and infrequent measurements in Extended Kalman Filter based nonlinear state estimation显示文摘 | Ajit Gopalakrishnan Niket S. Kaisare Shankar Narasimhan | 2010 | Journal of Process Control2010，，1： | 1 |
| 11 | Increased Fibroblast Growth Factor 21 in Obesity and Nonalcoholic Fatty Liver Disease显示文摘 | Jody Dushay Patricia C. Chui Gosala S. Gopalakrishnan Marta Varela–Rey Meghan Crawley Ffolliott M. Fisher Michael K. Badman Maria L. Martinez–Chantar Eleftheria Maratos–Flier | 2010 | Gastroenterology2010，，2： | 1 |
| 12 | Coincident idiopathic focal segmental glomerulosclerosis collapsing variant and diabetic nephropathy in an African American homozygous for MYH9 risk variants显示文摘 | Isai Gopalakrishnan Samy S. Iskandar Pirouz Daeihagh Jasmin Divers Carl D. Langefeld Donald W. Bowden Pamela J. Hicks Michael V. Rocco Barry I. Freedman | 2011 | Human Pathology2011，，2： | 1 |
| 13 | Surgical Management of Rectourethral Fistula显示文摘 | Gaurav Gupta Santosh Kumar Nitin S. Kekre Ganesh Gopalakrishnan | 2008 | Urology2008，，2： | 1 |
| 14 | Synthesis, mechanical, thermal and chemical properties of polyurethanes based on cardanol显示文摘 | C. V. Mythili A. Malar Retna S. Gopalakrishnan | 2004 | Bulletin of Materials Science2004，，3： | 1 |
| 15 | Jugular phlebectasia: theory of pathogenesis and review of literature显示文摘 | Vinidh Paleri S. Gopalakrishnan | 2001 | International Journal of Pediatric Otorhinolaryngology2001，，2： | 1 |
| 16 | High content screen microscopy analysis of Aβ 1–42 -induced neurite outgrowth reduction in rat primary cortical neurons: Neuroprotective effects of α7 neuronal nicotinic acetylcholine receptor ligands显示文摘 | Min Hu Mark E. Schurdak Pamela S. Puttfarcken Rachid El Kouhen Murali Gopalakrishnan Jinhe Li | 2007 | Brain Research2007，，： | 1 |
| 17 | Cloud Condensation Nuclei over the Bay of Bengal during the Indian Summer Monsoon显示文摘The first measurements of cloud condensation nuclei(CCN) at five supersaturations were carried out onboard the research vessel 'Sagar Kanya'(cruise SK-296) from the south to the head-bay of the Bay of Bengal as part of the Continental Tropical Convergence Zone(CTCZ) Project during the Indian summer monsoon of 2012. In this paper, we assess the diurnal variation in CCN distributions at supersaturations from 0.2% to 1%(in steps of 0.2%) and the power-law fit at supersaturation of 1%.The diurnal pattern shows peaks in CCN concentration(NCCN) at supersaturations from 0.2% to 1% between 0600 and 0700 LST(local standard time, UTC+0530), with relatively low concentrations between 1200 and 1400 LST, followed by a peak at around 1800 LST. The power-law fit for the CCN distribution at different supersaturation levels relates the empirical exponent(k) of supersaturation(%) and the NCCNat a supersaturation of 1%. The NCCNat a supersaturation of 0.4% is observed to vary from 702 cm^(-3) to 1289 cm^(-3), with a mean of 961 ± 161 cm^(-3)(95% confidence interval), representing the CCN activity of marine air masses. Whereas, the mean NCCNof 1628 ± 193 cm^(-3) at a supersaturation of 1% is higher than anticipated for the marine background. When the number of CCN spectra is 1293, the value of k is 0.57 ± 0.03(99% confidence interval)and its probability distribution shows cumulative counts significant at k ≈ 0.55 ± 0.25. The results are found to be better at representing the features of the marine environment(103 cm^(-3) and k ≈ 0.5) and useful for validating CCN closure studies for Indian sea regions. | D. M. CHATE R. T. WAGHMARE C. K. JENA V. GOPALAKRISHNAN P. MURUGAVEL Sachin D. GHUDE Rachana KULKARNI P. C. S. DEVARA | 2018 | Advances in Atmospheric Sciences2018，35，2： | 1 |
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