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| 1 | 用现行国产HBsAg EIA检测乙肝S基因突变体问题的研究显示文摘目的探讨乙型肝炎表面抗原(HBsAg)突变体对抗体结合能力的影响,寻找鉴别变异株的办法和规律,并为改进试剂质量进行基础研究。方法用23家国产HBsAgEIA检测10株重组表达HBsAg突变体和1株原型HBsAg。结果23家国产试剂均能检测出原型、重组表达HBsAg。表达的10株突变体,1号试剂可全部检出,其它试剂的检出率分别为:对突变体抗原145精氨酸、133亮氨酸、126天冬酰胺的检出率为91%,142丝氨酸为86%,142亮氨酸为82%,129组氨酸为78%,141谷氨酸为34%,126丝氨酸为30%,144丙氨酸为26%,145精氨酸+126丝氨酸为17%。结论乙肝S基因不同位点变异可导致与某些单抗的结合力降低,为进一步发展检测鉴别HBsAg突变体的诊断试剂提供了理论基础。 | 钟熙 王佑春 RLing 倪健 梁争论 祁自柏 TJHarrison 李河民 | 1998 | 中华微生物学和免疫学杂志1998,18,2: | 27 |
| 2 | 风生紊流导致微囊藻群体破碎和形态变化显示文摘微囊藻群体大小和形态决定其垂向迁移能力,从而影响着水华的形成.为了探讨湖泊中风生紊流对微囊藻群体大小和形态的影响,本研究于2012年8月26日至9月7日在太湖梅梁湾的围隔内进行了12 d的昼夜不间断的高频采样(采样间隔每2小时一次).研究期间,水面微囊藻密度呈现4次周期性消涨,藻密度变化范围为4×10^(4)~2671×10^(4)cells/m L.而整个水柱中的藻密度变化范围仅为3×10^(4)~18×10^(4)cells/m L.皮尔逊相关性分析表明微囊藻的原位生长速率与表面藻密度呈负相关而与风速呈正相关.强风速使微囊藻在水柱中均匀分散,增强了透光性,促进了微囊藻的生长.微囊藻群体粒径随着风速的增大逐渐减小,反之亦然.其中值粒径(D50)变化范围为66.2~768.0μm.在此期间微囊藻群体形态主要以鱼害微囊藻、不规则的惠氏微囊藻、球状的惠氏微囊藻和铜绿微囊藻群体形态为主,其占比也呈现出波动状态.皮尔逊相关分析结果显示微囊藻群体大小与风速呈负相关,说明湖泊中风生紊流会影响微囊藻群体大小.当紊流强度为2.33×10^(-5)m^(2)/s3时,微囊藻群体会发生破碎现象,该紊流强度相当于5 m/s的风在30 m深的水库或湖泊中所产生的紊流强度.微囊藻群体被风生紊流破碎后最大粒径与该风速下紊流的最小涡旋尺度相近,表明紊流的最小涡旋尺度决定了微囊藻所能形成群体的最终大小.监测期间,整水柱中不同群体形态的微囊藻占比发生了明显变化,在监测初期以鱼害微囊藻群体形态为主,随后不规则的惠氏微囊藻和铜绿微囊藻群体形态的比例不断增加,最后鱼害微囊藻群体形态又占据主导地位.球状的惠氏微囊藻群体形态在整个监测期中的比例随时间的增加而逐渐降低.不同群体形态微囊藻之间比例的大幅变化无法用微囊藻生长演替来解释.而皮尔逊相关分析结果显示鱼害微囊藻与惠氏微囊藻(不规则的和球状的惠氏微囊藻之和)群体形态之间存在负相关,且惠氏微囊藻与铜绿微囊藻群体形态呈负相关.但在今后研究中需进一步关注在微囊藻群体形态的动态变化过程中细胞大小、胶被、产毒特性和基因序列等特征,从而验证不同种微囊藻群体是否存在形态转换这一猜想.总而言之,普通强度的风生紊流能够破碎微囊藻群体,而气候变化导致的内陆湖泊周边风速下降会促使微囊藻形成更大的群体,从而有利于水华的形成. | 朱伟 陈怀民 肖曼 Lürling Miquel 李明 | 2021 | 湖泊科学2021,33,2: | 2 |
| 3 | 食物种类对萼花臂尾轮虫种群增长率的影响(英文)显示文摘在短期慢性观测过程中,食物类型可能是造成萼花臂尾轮虫(Brachionuscalyciflorus)种群繁殖率变化的一种原因。共观测了分别单独投喂10种不同绿藻对轮虫种群增长率的影响。为验证藻青菌是藻类饵料(如绿藻Scenedesmus)有价值的佐剂这一假说,还用蓝细菌单独投喂或与斜生栅藻(Scenedesmusobliquus)混合投喂轮虫进行实验观测。结果发现食物种类对萼花臂尾轮虫种群增长率影响显著。斜生栅藻组获得最大种群增长(1.6/d),而Desmodesmus组增长率最低(0.3/d)。以占斜生栅藻组最大增长的百分率来表示,其它几种绿藻组种群增长由高到低依次为:Desmodesmussubspicatus88%,小球藻(Chlorellavulgaris)83%,单壳缝藻(Monoraphidiumminutum)77%,D.quadricauda74%,S.falcatus71%,S.acuminatus69%,S.pectinatus64%,莱茵衣藻(Chlamydomonasreinhardtii)57%,D.abundans19%。轮虫增长率的差异不能用藻类饵料的大小差异来解释。蓝细菌(Microcystisaeruginosa)和(Synechococcuselongates)不论是单独投喂还是与优良藻类饵料(斜生栅藻)混合投喂都对萼花臂尾轮虫种群增长有抑制作用。这种副作用似乎与微囊藻素无关。该结果不支持无毒蓝细菌可作为与其他绿藻饵料配合使用的优良佐剂这一假说。本研究所观察到的生长变化显示了饵料种类对萼花臂尾轮虫种群增长的影响。 | M.LüRLING W.BEEKMAN | 2006 | 动物学报2006,52,1: | 2 |
| 4 | Antioxidant treatment with N-acet ylcyst eine and vitamin C in patients with chronic bronchitis显示文摘 | Lukas R Scha rling B Sehultze Weminghaus G | 2005 | Dt sch Med Wochensehr2005,130,11: | 1 |
| 5 | Empirical tests of a model of determinants of scriptbased driving choice显示文摘 | G?RLING T FUJII S BOE O | 2001 | Transportation Research Part F:Traffic Psychology and Behaviour2001,4,2: | 1 |
| 6 | The relationships between awareness of consequences, environmental concern, and value orientations显示文摘 | André Hansla Amelie Gamble Asgeir Juliusson Tommy G?rling | 2007 | Journal of Environmental Psychology2007,,1: | 1 |
| 7 | Application of attitude theory for improved predictive accuracy of stated preference methods in travel demand analysis显示文摘 | Satoshi Fujii Tommy G(a)rling | | 0,,: | 1 |
| 8 | Moderating effects of social value orientation on determinants of proenvironmental behavior intention显示文摘 | Tommy G?rling Satoshi Fujii Anita G?rling Cecilia Jakobsson | 2003 | Journal of Environmental Psychology2003,,1: | 1 |
| 9 | Selective Attention and Heart Rate Responses to Natural and Urban Environments显示文摘 | Laumann K G?rling T Stormark K M | 2003 | Journal of Environmental Psychology2003,23,2: | 1 |
| 10 | Interaction effects of mood induction and nominal representation of price on consumer choice显示文摘 | AMELIE GAMBLE TOMMY GA' RLING DANIEL VA'STFJA' LL AGNETA MARELL | | 0,,: | 1 |
| 11 | Rating Scale Measures of Restorative Components of Environments显示文摘 | Laumann K G?rling T Stormark K M | 2001 | Journal of Environmental Psychology2001,21,1: | 1 |
| 12 | Recombinant human Fab fragments neutralizehuman type 1 immunodeficiency virus in vitro显示文摘 | Barbas CF 3rd Bjrling E Chiodi F | 1992 | Proc Natl Acad Sci1992,89,19: | 1 |
| 13 | Reintroducing attitude theory in travel behavior research: The validity of an interactive interview procedure to predict car use显示文摘 | Tommy G?rling Robert Gillholm Anita G?rling | 1998 | Transportation1998,,2: | 1 |
| 14 | Moderating effects of social value orientation on determinants of proenvironmental behavior intention显示文摘 | G(a)rling T Fujii S G.rling A Jakobsson C | | 0,,01: | 1 |
| 15 | Daphnia growth on microcystin-producing and microcystin-free Microcystis aeruginosa in different mixtures with the green alga Scenedesmus obliquus 显示文摘 | Lürling M | 2003 | Limnol Oceanogr2003,48,6: | 1 |
| 16 | Effects of the cyanobacterium Cylindrospermopsis raciborskii on feeding and life-history characteristics of the grazer Daphnia magna 显示文摘 | Soares MCS Lürling M Panosso R | 2009 | Ecotoxicol Environ Saf2009,72,4: | 1 |
| 17 | Contributions of the Cerebellum to Disturbed Central Processing of Visceral Stimuli in Irritable Bowel Syndrome显示文摘 | Christina Rosenberger Markus Thürling Michael Forsting Sigrid Elsenbruch Dagmar Timmann Elke R. Gizewski | 2013 | The Cerebellum2013,,2: | 1 |
| 18 | House-hold choices of car-use reduction measures显示文摘 | Tommy G rling Anita G rling Anders Johansson | | 0,,: | 1 |
| 19 | An Analysis of International Business-to-Business Relationships Based on the Commitment--Trust Theory 显示文摘 | FRIMAN M G~RLING T MILLETT B | 2002 | Industrial Marketing Management2002,31,5: | 1 |
| 20 | Phosphorus leaching in relation to soil type and soil phosphorus content显示文摘 | Djodjic Faruk Brling Katarina Bergstrm Lars | 2004 | J Environ Qual2004,33,: | 1 |