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| 1 | Insights into Cronobacter sakazakii Biofilm Formation and Control Strategies in the Food Industry显示文摘Cronobacter sakazakii(C.sakazakii)is a foodborne opportunistic pathogen that can cause life-threatening invasive diseases,such as necrotizing enterocolitis,meningitis,and sepsis in infants.The potential risk of C.sakazakii contamination of powdered infant formula(PIF)is an issue that has attracted considerable attention from manufacturers,regulators,and consumers.C.sakazakii biofilms on the surfaces of equipment and in diverse food-production environments constitute a mode of cell growth that protects the pathogen from hostile environments,and are an important source of persistent contamination of food products.Bacterial biofilms are difficult to remove due to their resistant properties.Conventional cleaning and sterilizing procedures may be insufficient for biofilm control,and may lead to further biofilm development and dispersal.Consequently,novel control strategies are being developed,such as nanotechnology-based delivery systems,interspecies interactions,antimicrobial molecules of microbial origin,natural extracts,and phages.This review focuses on describing the mechanisms underlying the biofilm formation and behavior of C.sakazakii and discussing potential control strategies. | Na Ling Stephen Forsythe Qingping Wu Yu Ding Jumei Zhang Haiyan Zeng | 2020 | Engineering2020，6，4： | 10 |
| 2 | Simultaneously Enhanced Cryogenic Tensile Strength,Ductility and Impact Resistance of Epoxy Resins by Polyethylene Glycol显示文摘Rubbers have been well accepted for modifying brittle epoxies but rubber modified epoxies usually posses lowered tensile strength though enhanced ductility and fracture resistance.In this work,a polyethylene glycol(PEG-4000) is used to modify diglycidyl ether of bisphenol A/methyltetrahydrophthalic anhydride system for enhancing cryogenic tensile strength,ductility and impact resistance.The results display that the cryogenic tensile strength,ductility(failure strain) and fracture resistance(impact strength) are all enhanced for the modified epoxy system at proper PEG contents.The maximum tensile strength(127.8 MPa) at the cryogenic temperature(77 K) with an improvement of 30.1%is observed for the modified system with the 15 wt%PEG content.The ductility and impact resistance at both room temperature and cryogenic temperature are all improved for the modified epoxy system with proper PEG-4000 contents.These observations are explained by the positron annihilation lifetime spectroscopy results and scanning electron microscopy results.Moreover,the glass transition temperature decreases slightly with increasing PEG content. | Qingping Feng Jiaoping Yang Yu Liu Hongmei Xiao Shaoyun Fu | 2014 | Journal of Materials Science & Technology2014，30，1： | 6 |
| 3 | Nuclear Aurora kinase A switches m^(6)A reader YTHDC1 to enhance an oncogenic RNA splicing of tumor suppressor RBM4显示文摘Aberrant RNA splicing produces alternative isoforms of genes to facilitate tumor progression,yet how this process is regulated by oncogenic signal remains largely unknown.Here,we unveil that non-canonical activation of nuclear AURKA promotes an oncogenic RNA splicing of tumor suppressor RBM4 directed by m^(6)A reader YTHDC1 in lung cancer.Nuclear translocation of AURKA is a prerequisite for RNA aberrant splicing,specifically triggering RBM4 splicing from the full isoform(RBM4-FL)to the short isoform(RBM4-S)in a kinase-independent manner. | SiSi Li YangFan Qi JiaChuan Yu YuChao Hao Bin He MengJuan Zhang ZhenWei Dai TongHui Jiang SuYi Li Fang Huang Ning Chen Jing Wang MengYing Yang DaPeng Liang Fan An JinYao Zhao WenJun Fan YuJia Pan ZiQian Deng YuanYuan Luo Tao Guo Fei Peng ZhiJie Hou ChunLi Wang FeiMeng Zheng LingZhi Xu Jie Xu QingPing Wen BiLian Jin Yang Wang Quentin Liu | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 4 |
| 4 | Alteration of P2X3 expression in dorsal root ganglia after sciatic nerve ligation显示文摘BACKGROUND:The expressions of P2X3 receptor in dorsal root ganglia(DRG)after different peripheral nerve injuries are diverse.It indicates the different roles of P2X3 in different models-caused neuropathologic pains.OBJECTIVE:To observe the expressions of P2X3 in corresponding DRG after sciatic nerve ligation in rats.DESIGN:Controlled observation experiment.SETTING:Department of Morphology,Hunan Traditional Chinese Medical College;Department of Human Anatomy and Neurobiology,Xiangya Medical College,Central South University.MATERIALS:Thirty-five healthy adult SD rats of clean grade an d either gender,weighing(200±20)g,were involved.According to the random digits table,the involved rats were randomized into 3 groups:normal group(n =5),sham-operated group(n =5)and experimental group(n =25).The experimental group were subdivided into 3,7,14,21,28 days groups according to different surviving time after operation,5 rats at each time point.Polyclonal rabbit anti-P2X3 antibody(ABCAM company);biotinylated goat anti-rabbit IgG(Zhongshanjingqiao Biotechnical Co.,Ltd.,Beijing);Motic fluorescence microscope(Motic,Germany).METHODS:The experiments were carried out in the Department of Human Anatomy and Neurobiology,Xiangya Medical College,Central South University from June to December 2006.① Rats of experimental group were created into models by ligation of right sciatic nerve according to the method of Seltzer et al.Left sciatic nerve was used as self-control.As for rats in the sham-operated group,ligation of sciatic nerve was omitted,but other procedures were the same as those in the experimental group.Rats of normal group were untouched.② Rats of the normal group and sham-operated group survived for 14 days separately,and those of experimental group survived for corresponding time.After being deeply anesthetized by intraperitoneal injection of over-dose sodium pentobarbital,the rats of experimental group were transcardially perfused.L4-6 corresponding DRG connected to sciatic nerve were taken for preparing transverse sections serially.③P2X3 expression in L4-6 DRG was detected by immunohistochemistry,immunofluorescence and image analysis techniques.MAIN OUTCOME MEASURES:P2X3 expression in L4-6 DRG of rats in each group.RESULTS:Thirty-five SD rats were involved in the final analysis.① P2X3 expression in DRG:In normal DRG of rats,there were abundant P2X3 immuno-positive small-and medium-sized primary sensory neurons,especially the small ones,which mostly received the input from C fibers.There were only a few large neurons expressing P2X3.The immuno-positive products mostly were located in the cytoplasm and processes.The expression of P2X3 had a slight but significant decrease in ipsilateral L4-6 DRG 3 days after sciatic nerve ligation,and a decreasing tendency was observed with the elongation of time.At 28 days,the expression had not returned to base line,and still maintained at a low level.② P2X3 immuno-positive gray scale in DRG:P2X3 immuno-positive gray scale in ipsilateral side L4-6 DRG was 117.74±2.38,129.12± 4.86,133.56±3.79,148.75±6.90 and 150.49±5.15,respectively at 3,7,14,21 and 28 days after sciatic nerve ligation,which was significantly higher than that in the normal group and sham-operated group(105.11±3.52,104.22±5.41,F =78.861,P < 0.05),also significantly higher than that in the contralateral side(105.53±5.85,108.54±3.70,104.07±4.16,106.55±2.02,106.29±5.19,t =3.48-13.95,P < 0.05);There were no significant differences when comparing sham-operated group or contralateral side at each time point with normal group(P > 0.05)CONCLUSION:P2X3 is significantly down regulated in L4-6 DRG after sciatic nerve ligation.It may exert certain effects in neuropathic pain. | Guoxing Zhou Lesi Xie Qiben Wang Qingping Yu Xiaofu Liu Qiumei Liu Wei Peng Zhicheng Zeng | 2007 | Neural Regeneration Research2007，2，4： | 3 |
| 5 | The detection of tetrahydrofuran hydrate formation and saturation using magnetic resonance imaging technique显示文摘Tetrahydrofuran(THF) hydrate was formed in bulk as well as in glass beads pack with a mean diameter of 3.0 mm by controlling the temperature under ambient pressure.Images of THF hydrate formation procedure were obtained using the magnetic resonance imaging(MRI) technique.The experiment results showed that MRI is an effective method for the detection of hydrate formation.Saturation of hydrate formed both in bulk and glass beads can be confirmed by intensity integration of MRI images. | Yu Liu Yongchen Song Yongjun Chen Lei Yao Qingping Li | 2010 | Journal of Natural Gas Chemistry2010，19，3： | 2 |
| 6 | Reduction-induced interface reconstruction to fabricate MoNi_(4) - based hollow nanorods for hydrazine oxidation assisted energysaving hydrogen production in seawater显示文摘Seawater electrolysis could address the water scarcity issue and realize the grid-scale production of carbon-neutral hydrogen,while facing the challenge of high energy consumption and chloride corrosion.Thermodynamically more favorable hydrazine oxidation reaction(HzOR)assisted water electrolysis is efficiency for energy-saving and chlorine-free hydrogen production.Herein,the MoNi alloys supported on MoO_(2) nanorods with enlarged hollow diameter on Ni foam(MoNi@NF)are synthesized,which is constructed by limiting the outward diffusion of Ni via annealing and thermal reduction of NiMoO_(4) nanorods.When coupling HzOR and hydrogen evolution reaction(HER)by employing MoNi@NF as both anode and cathode in two-electrode seawater system,a low cell voltage of 0.54 V is required to achieve 1,000 mA·cm^(−2) and with long-term durability for 100 h to keep above 100 mA·cm^(−2) and nearly 100%Faradaic efficiency.It can save 2.94 W·h to generate per liter H_(2) relative to alkaline seawater electrolysis with 37%lower energy equivalent input. | Lili Guo Qingping Yu Xuejun Zhai Jingqi Chi Tong Cui Yu Zhang Jianping Lai Lei Wang | 2022 | Nano Research2022，15，10： | 2 |
| 7 | Food Safety Risks and Contributing Factors of Cronobacter spp.显示文摘Cronobacter species are a group of Gram-negative opportunistic pathogens,which cause meningitis,sep-ticemia,and necrotizing enterocolitis in neonates and infants,with neurological sequelae in severe cases.Interest in Cronobacter has increased significantly in recent years due to its high virulence in children.In this review,we summarize the current understanding of the prevalence of Cronobacter species in several important food types.We discuss the response mechanisms enabling persistence in adverse growth con-ditions,as well as its pathogenicity.We emphasize the food safety concerns caused by Cronobacter and subsequent control methods and clinical treatments. | Na Ling Xiuting Jiang Stephen Forsythe Danfeng Zhang Yizhong Shen Yu Ding Juan Wang Jumei Zhang Qingping Wu Yingwang Ye | 2022 | Engineering2022，8，5： | 2 |
| 8 | 3D imaging application in the studies of micro air vehicles 显示文摘 | Yanan Yu Qingping Yang Xiangjun Wang | 2013 | Computers in Industry (S0166-3615)2013，64，9： | 1 |
| 9 | Finite element analysis of fluid-structure interaction for the design of MAV aerodynamic shape 显示文摘 | Yanan Yu Qingping Yang Xiangjun Wang | 2013 | Computers and Fluids (S0045-7930)2013，76，10： | 1 |
| 10 | A MICROMECHANICS CONSTITUTIVE THEORY FOR FORWARD TRANSFORMATION PLASTICITY WITH SHEAR AND DILATATION EFFECT:I,NONPROPORTIONAL LOADING HISTORY显示文摘A micromechanics constitutive theory which takes into account both the dilatation and shear ef-fects of the transformation is proposed to describe the macroscopic plastic behavior of structure ceramics duringforward transformation under different temperatures.Under some basic assumptions,the analytic expressions ofthe Helmholtz and complementary free energy of the constitutive element are derived in a self-consistentmanner by using the Mori-Tanaka’s method which takes into account the interaction between the transformedinclusions.In the framework of Hill-Rice’s internal variable constitutive theory,the forward transformationyield function and incremental stress strain relations,in analogy to the theory of metal plasticity,fornon-proportional loading histories are obtained. | Hwang Kehchih Sun Qingping Yu Shouwen (Department of Engineering Mechanics,Tsinghua University,Beijing,China.) | 1990 | Acta Mechanica Sinica1990，6，4： | 1 |
| 11 | Protection of exendin-4 analogue in early experimental diabetic retinopathy显示文摘 | Yu Zhang Qingping Wang Jingfa Zhang Xia Lei Guo-Tong Xu Wen Ye | 2009 | Graefe’s Archive for Clinical and Experimental Ophthalmology2009，，5： | 1 |
| 12 | A MICROMECHANICS CONSTITUTIVE MODEL FOR PURE DILATANT MARTENSITIC TRANSFORMATION OF ZrO2 CONTAINING CERAMICS显示文摘A new micromechanics constitutive model for pure dilatant transformation plasticity of structureceramics is proposed in this paper.Based on the thermodynamics,micromechanics and microscale t→ m trans-formation mechanism analysis of the TZP and PSZ ZrO2-containing ceramics,an analytic expressions of theHelmholtz and complementary free energy of the constitutive element for the case of pure dilatant transforma-tion is derived for the first time in a self-consistent manner.By the analysis of energy dissipation in the for-ward and reverse transformations,the mieromechanics constitutive law is derived in the framework of Hill-Rice’sinternal variable constitutive theory. | Sun Qingping Yu Shouwen Hwang Kehchih (Dept.of Engng.Mech.,Tsinghua University,Beijing,China) | 1990 | Acta Mechanica Sinica1990，6，2： | 1 |
| 13 | Protection of exendin-4 analogue in early experimental diabetic retinopathy显示文摘 | Yu Zhang Qingping Wang Jingfa Zhang Xia Lei Guo-Tong Xu Wen Ye | 2009 | Graefe’s Archive for Clinical and Experimental Ophthalmology2009，，5： | 1 |
| 14 | Abnormal expression of matrix metalloproteinase-9 (MMP9)correlates with clinical course in Chinese patients with endometrialcancer显示文摘 | Fang Yu Qingping Jiang Ying Zhou Zhen Yang Xiaoli Yu Hao Wang Zhen Liu Lijing Wang Weiyi Fang Suiqun Guo | 2012 | Disease Markers2012，，5： | 1 |
| 15 | Steering Against Wind: A New Network of NamiRNAs and Enhancers显示文摘Micro RNAs(miRNAs) are a class of endogenous non-coding RNAs with regulatory functions. Traditionally, miRNAs are thought to play a negative regulatory role in the cytoplasm by binding to the 30 UTR of target genes to degrade m RNA or inhibit translation. However, it remains a challenge to interpret the potential function of many miRNAs located in the nucleus.Recently, we reported a new type of miRNAs present in the nucleus, which can activate gene expression by binding to the enhancer, and named them nuclear activating miRNAs(NamiRNAs). The discovery of NamiRNAs showcases a complementary regulatory mechanism of mi RNA, demonstrating their differential roles in the nucleus and cytoplasm. Here, we reviewed miRNAs in nucleus to better understand the function of NamiRNAs in their interactions with the enhancers. Accordingly, we propose a Nami RNA–enhancer–target gene activation network model to better understand the crosstalk between NamiRNAs and enhancers in regulating gene transcription.Moreover, we hypothesize that NamiRNAs may be involved in cell identity or cell fate determination during development, although further study is needed to elucidate the underlying mechanisms in detail. | ying liang qingping zou wenqiang yu | 2017 | Genomics, Proteomics & Bioinformatics2017，15，5： | 1 |
| 16 | Quantum Dot Nanobeads-Labelled Lateral Flow Immunoassay Strip for Rapid and Sensitive Detection of Salmonella Typhimurium Based on Strand Displacement Loop-Mediated Isothermal Amplification显示文摘Rapid,sensitive,point-of-care detection of pathogenic bacteria is important for food safety.In this study,we developed a novel quantum dot nanobeads-labelled lateral flow immunoassay strip(QBs-labelled LFIAS)combined with strand displacement loop-mediated isothermal amplification(SD-LAMP)for quantitative Salmonella Typhimurium(ST)detection.Quantum dot nanobeads(QBs)served as fluorescence reporters,providing good detection efficiency.The customizable strand displacement(SD)probe was used in LAMP to improve the specificity of the method and prevent by-product capture.Detection was based on a sandwich immunoassay.A fluorescence strip reader measured the fluorescence intensity(FI)of the test(T)line and control(C)line.The linear detection range of the strip was 10^(2)–10^(8) colony forming units(CFU)·mL^(-1).The visual limit of detection was 10^(3) CFU·mL^(-1),indicating that the system was ten-fold more sensitive than AuNPs-labelled test strips.ST specificity was analyzed in accordance with agarose gel outputs of polymerase chain reaction(PCR)and SD-LAMP.We detected ST in foods with an acceptable recovery of 85%–110%.The method is rapid,simple,almost equipment-free,and suitable for bacterial detection in foods and for clinical diagnosis. | Yuting Shang Shuzhen Cai Qinghua Ye Qingping Wu Yanna Shao Xiaoying Qu Xinran Xiang Baoqing Zhou Yu Ding Moutong Chen Liang Xue Honghui Zhu Jumei Zhang | 2022 | Engineering2022，，12： | 0 |
| 17 | 睾丸的原发性NK/T细胞淋巴瘤一例报告及文献复习(英文)显示文摘Objective: To study the clinical and pathological features of primary NK/T cell lymphoma of testis and to investi- gate the effective diagnosis and treatment of this disease. Methods: The surgical specimens of a patient with primary NK/T cell lymphoma of the testis were observed by light microscopy, immunohistochemistry and examined by the polymerase chain reaction (PCR) for Epstein-Barr virus (EBV) DNA and T-cell receptor (TCR) gene rearrangement, and the literature were reviewed. Results: The patient presented with left-sided painless testicular enlargement and the lymphoma had a propensity to spread to the contralateral testis, spleen, central nervous system, and so on. The neoplastic cells were positive for CD56, CD45R0 and CD3ε, while the expressions of CD20, CD79α, CD5, Bcl-2 and PLAP were negative. In addition, the EBV DNA was detected in the lymphoma by PCR. And the results of gene rearrangement studies for the γ chain of the T-cell receptor were negative. The pathological diagnosis was NK/T cell lymphoma of the left testis. Conclusion: Primary NK/T cell lym- phoma of the testis is a rare entity and progressed rapidly. The histopathological, immunohistochemical, EBV examination and TCR gene rearrangement studies should be carried out as soon as possible in order to get the defined diagnosis. Currently, the therapeutic efficacy is poor and the new measures should be investigated to improve the survival rate. | Qinbing Zeng Fanjun Cheng Qingping Gao Zhengpeng Zhu Qihuan Liu Qiang Yu Jian Lu | 2007 | The Chinese-German Journal of Clinical Oncology2007，6，6： | 0 |
| 18 | Nonalcoholic steatohepatitis critically rewires the ischemia/ reperfusion-induced dysregulation of cardiolipins and sphingolipids in mice显示文摘Background:Lipid dysregulation plays a fundamental role in nonalcoholic steatohepatitis(NASH),which is an emerging critical risk factor that aggravates hepatic ischemia/reperfusion(I/R)injury.However,the specific lipids that mediate the aggressive I/R injury in NASH livers have not yet been identified.Methods:The mouse model of hepatic I/R injury on NASH was established on C56B/6J mice by first feeding the mice with a Western-style diet to induce NASH,then the NASH mice were subjected to surgical procedures to induce hepatic I/R injury.Untargeted lipidomics were performed to determine hepatic lipids in NASH livers with I/R injury through ultra-high performance liquid chromatography coupled with mass spectrometry.The pathology associated with the dysregulated lipids was examined.Results:Lipidomics analyses identified cardiolipins(CL)and sphingolipids(SL),including ceramides(CER),glycosphingolipids,sphingosines,and sphingomyelins,as the most relevant lipid classes that characterized the lipid dysregulation in NASH livers with I/R injury.CER were increased in normal livers with I/R injury,and the I/R-induced increase of CER was further augmented in NASH livers.Metabolic pathway analysis revealed that the enzymes involved in the synthesis and degradation of CER were highly upregulated in NASH livers with I/R injury,including serine palmitoyltransferase 3(Sptlc3),ceramide synthase 2(Cers2),neutral sphingomyelinase 2(Smpd3),and glucosylceramidase beta 2(Gba2)that produced CER,and alkaline ceramidase 2(Acer2),alkaline ceramidase 3(Acer3),sphingosine kinase 1(Sphk1),sphingosine-1-phosphate lyase(Sgpl1),and sphingosine-1-phosphate phosphatase 1(Sgpp1)that catalyzed the degradation of CER.CL were not affected by I/R challenge in normal livers,but CL was dramatically reduced in NASH livers with I/R injury.Consistently,metabolic pathway analyses revealed that the enzymes catalyzing the generation of CL were downregulated in NASH-I/R injury,including cardiolipin synthase(Crls1)and tafazzin(Taz).Notably,the I/R-induced oxidative stress and cell death were found to be aggravated in NASH livers,which were possibly mediated by the reduction of CL and accumulation of CER.Conclusions:The I/R-induced dysregulation of CL and SL were critically rewired by NASH,which might potentially mediate the aggressive I/R injury in NASH livers. | Sheng Yu Kai Wang Qingping Li Yiran Wei Yiyi Li Qifan Zhang Pengxiang Huang Hanbiao Liang Hang Sun Hongxian Peng Xixin Huang Cuiting Liu Jie Zhou Jianping Qian Chuanjiang Li | 2023 | Hepatobiliary Surgery and Nutrition2023，12，1： | 0 |
| 19 | Experimental simulation of gas hydrate decomposition in porous sediment显示文摘Gas hydrate decomposition in sediments involves complicated multiphase flow and heat and mass transfer processes because of heat absorption by solid hydrates. Factors affecting gas hydrate decomposition in sediments include sediment type, mineral composition, pore size distribution, particle size, pore water composition, hydrate saturation distribution, initial formation pressure and temperature and cement characteristics. In this paper, experimental simulations of gas hydrate decomposition are carried out on an artificial core to investigate the effects of initial pressure and temperature, particle size and pore size. The experiments show that the characteristics of gas hydrate decomposition in sediments differ completely from those in a pure water system. The decomposition rate of hydrate sediments increases with the initial pressure increasing and decreasing temperatures. Furthermore, the decomposition rate of hydrate sediments decreases with decreasing particle size and increasing pore size. | YU XiChong LI Gang LI QingPing LI XiaoSen ZHANG Yu PANG WeiXin BAI YuHu | 2013 | Science China Earth Sciences2013，56，4： | 0 |
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