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| 1 | UHPLC-MS/MS analysis of cAMP and cGMP in rat plasma as potential biomarkers of Yin-Yang disharmony in traditional Chinese medicine显示文摘Cyclic 3’,5’-adenosine monophosphate(cAMP)and cyclic 3’,5’-guanosine monophosphate(cGMP)are considered as potential biomarkers for Yin-Yang disharmony in traditional Chinese medicine.However,phosphodiesterase-mediated ex vivo degradation of these molecules in biological samples may result in their underestimation.In the present study,a ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)method was developed for determination of cAMP and cGMP in rat plasma,with special consideration of their stability ex vivo.Following precipitation of proteins from plasma samples with 0.4 M perchloric acid,the analytes were chromatographed on a Shimadzu Shimpack-XR-ODS II column with 2.5 mM ammonium acetate and methanol in gradient mode.The MS/MS detection was performed using multiple reaction monitoring in the positive electrospray ionization mode.The lower limit of quantification was 0.27 ng/mL for cAMP and 0.37 ng/mL for cGMP.The method was used to determine the plasma cAMP and cGMP levels in normal and Yin deficiency diabetic rats treated with or without Rehmannia glutinosa.The developed method may be useful for evaluating the regulatory effects of Chinese herbal medicine on the levels of cAMP and cGMP in the body. | Xin Wang Yue Du Cuiting Wu Ming Xu Youping Liu Xin Di | 2021 | Journal of Pharmaceutical Analysis2021，11，4： | 3 |
| 2 | Ultra-short-course and intermittent TB47-containing oral regimens produce stable cure against Buruli ulcer in a murine model and prevent the emergence of resistance for Mycobacterium ulcerans显示文摘Buruli ulcer(BU),caused by Mycobacterium ulcerans,is currently treated with rifampin estreptomycin or rifampineclarithromycin daily for 8 weeks recommended by World Health Organization(WHO).These options are lengthy with severe side effects.A new anti-tuberculosis drug,TB47,targeting QcrB in cytochrome bc1:aa3 complex is being developed in China.TB47-containing regimens were evaluated in a well-established murine model using an autoluminescent M.ulcerans strain.Highlevel TB47-resistant spontaneous M.ulcerans mutants were selected and their qcrB genes were sequenced.The in vivo activities of TB47 against both low-level and high-level TB47-resistant mutants were tested in BU murine model.Here,we show that TB47-containing oral 3-drug regimens can completely cure BU in 2 weeks for daily use or in 3 weeks given twice per week(6 doses in total).All high-level TB47-resistant mutants could only be selected using the low-level mutants which were still sensitive to TB47 in mice.This is the first report of double mutations in QcrB in mycobacteria.In summary,TB47-containing regimens have promise to cure BU highly effectively and prevent the emergence of drug resistance.Novel QcrB mutations found here may guide the potential clinical molecular diagnosis of resistance and the discovery of new drugs against the high-level resistant mutants. | Yamin Gao HMAdnan Hameed Yang Liu Lingmin Guo Cuiting Fang Xirong Tian Zhiyong Liu Shuai Wang Zhili Lu Md Mahmudul Islam Tianyu Zhang | 2021 | Acta Pharmaceutica Sinica B2021，11，3： | 0 |
| 3 | Catalytic adsorptive desulfurization of mercaptan,sulfide and disulfide using bifunctional Ti-based adsorbent for ultra-clean oil显示文摘Ultra-deep desulfurization of transformer oil is of great demand among power industry.In this work,the effective and deep removal of various types of organosulfurs,including mercaptan,sulfide and disulfide via catalytic adsorptive desulfurization(CADS)using bifunctional Ti-based adsorbent is reported.Compared to adsorptive desulfurization(ADS),dramatically improvement of the organosulfur uptakes were achieved under CADS process.The equilibrium adsorption capacity at 5 μg∙g^(-1) S reached up to 15.7,33.4,11.6 and 11.9 mg∙g^(-1) for propyl mercaptan(n-PM),dimethyl sulfide(DMS),di-t-butyl disulfide(DTBDS)and dibenzyl disulfide(DBDS),which was 262,477,97 and 128 times to that of ADS process,respectively,and was the highest among the reported desulfurization adsorbents.Moreover,it achieved superior breakthrough capacity of 2050,530 and 210 ml F·(g A)^(-1) at the breakthrough S concentration of 1 μg∙g^(-1) of the commercial transformer oil S containing 10,50 and 150 μg∙g^(-1),respectively.The effectiveness of CADS is associated to the transformation of sulfur species to higher polar sulfonic species with the assistance of mild oxidant,which can be readily captured by silanol groups on SiO2 through Hbonding interaction.The excellent recyclability of the adsorbent can be realized through solvent washing or oxidative air treatment.This work provides an effective and economic approach for the elimination of trace amount of mercaptan,sulfide and disulfide from transformer oil. | Cuiting Yang Bowen Wu Zewei Liu Guang Miao Qibin Xia Zhong Li Michael J.Janik Guoqing Li Jing Xiao | 2022 | Chinese Journal of Chemical Engineering2022，35，2： | 0 |
| 4 | Nonalcoholic steatohepatitis critically rewires the ischemia/ reperfusion-induced dysregulation of cardiolipins and sphingolipids in mice显示文摘Background:Lipid dysregulation plays a fundamental role in nonalcoholic steatohepatitis(NASH),which is an emerging critical risk factor that aggravates hepatic ischemia/reperfusion(I/R)injury.However,the specific lipids that mediate the aggressive I/R injury in NASH livers have not yet been identified.Methods:The mouse model of hepatic I/R injury on NASH was established on C56B/6J mice by first feeding the mice with a Western-style diet to induce NASH,then the NASH mice were subjected to surgical procedures to induce hepatic I/R injury.Untargeted lipidomics were performed to determine hepatic lipids in NASH livers with I/R injury through ultra-high performance liquid chromatography coupled with mass spectrometry.The pathology associated with the dysregulated lipids was examined.Results:Lipidomics analyses identified cardiolipins(CL)and sphingolipids(SL),including ceramides(CER),glycosphingolipids,sphingosines,and sphingomyelins,as the most relevant lipid classes that characterized the lipid dysregulation in NASH livers with I/R injury.CER were increased in normal livers with I/R injury,and the I/R-induced increase of CER was further augmented in NASH livers.Metabolic pathway analysis revealed that the enzymes involved in the synthesis and degradation of CER were highly upregulated in NASH livers with I/R injury,including serine palmitoyltransferase 3(Sptlc3),ceramide synthase 2(Cers2),neutral sphingomyelinase 2(Smpd3),and glucosylceramidase beta 2(Gba2)that produced CER,and alkaline ceramidase 2(Acer2),alkaline ceramidase 3(Acer3),sphingosine kinase 1(Sphk1),sphingosine-1-phosphate lyase(Sgpl1),and sphingosine-1-phosphate phosphatase 1(Sgpp1)that catalyzed the degradation of CER.CL were not affected by I/R challenge in normal livers,but CL was dramatically reduced in NASH livers with I/R injury.Consistently,metabolic pathway analyses revealed that the enzymes catalyzing the generation of CL were downregulated in NASH-I/R injury,including cardiolipin synthase(Crls1)and tafazzin(Taz).Notably,the I/R-induced oxidative stress and cell death were found to be aggravated in NASH livers,which were possibly mediated by the reduction of CL and accumulation of CER.Conclusions:The I/R-induced dysregulation of CL and SL were critically rewired by NASH,which might potentially mediate the aggressive I/R injury in NASH livers. | Sheng Yu Kai Wang Qingping Li Yiran Wei Yiyi Li Qifan Zhang Pengxiang Huang Hanbiao Liang Hang Sun Hongxian Peng Xixin Huang Cuiting Liu Jie Zhou Jianping Qian Chuanjiang Li | 2023 | Hepatobiliary Surgery and Nutrition2023，12，1： | 0 |
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