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| 1 | Comparison of lentiviruses pseudotyped with S proteins from coronaviruses and cell tropisms of porcine coronaviruses显示文摘The surface glycoproteins of coronaviruses play an important role in receptor binding and cell entry. Different coronaviruses interact with their specific receptors to enter host cells. Lentiviruses pseudotyped with their spike proteins(S) were compared to analyze the entry efficiency of various coronaviruses. Our results indicated that S proteins from different coronaviruses displayed varied abilities to mediate pseudotyped virus infection. Furthermore, the cell tropisms of porcine epidemic diarrhea virus(PEDV) and transmissible gastroenteritis virus(TGEV) have been characterized by live and pseudotyped viruses. Both live and pseudoviruses could infected VeroCCL-81(monkey kidney), Huh-7(human liver), and PK-15(pig kidney) cells efficiently. CCL94(cat kidney) cells could be infected efficiently by TGEV but not PEDV. Overall, our study provides new insights into the mechanisms of viral entry and forms a basis for antiviral drug screening. | Jingjing Wang Feng Deng Gang Ye Wanyu Dong Anjun Zheng Qigai He Guiqing Peng | 2016 | Virologica Sinica2016，31，1： | 2 |
| 2 | Inhibition of porcine circovirus type 1 and type 2 production in PK-15 cells by small interfering RNAs targeting the Rep gene显示文摘 | Minxuan Sun Xueqin Liu Shengbo Cao Qigai He Rui Zhou Jing Ye Yaoming Li Huanchun Chen | 2007 | Veterinary Microbiology2007，，1： | 1 |
| 3 | The development and application of the Latex agglutination test to detect sera antibodies against Japanese encephalitis virus显示文摘 | Jia xinglin Chen Huanchun He qigai | 2002 | Veternary Resaerch Communication2002，26，6： | 1 |
| 4 | Characterization of monoelonal antibody against SARS eoronavirus nucleoeapsid antigen and development of an antigen capture ELISA显示文摘 | He Qigai Du Qingyun Lau Suelyn | 2005 | Journal of Virological Methods2005，129，： | 1 |
| 5 | Molecular characterization, induced expression, and transcriptional regulation of porcine S100A12 gene显示文摘 | Hongbo Chen Lei Cheng Songbai Yang Xiangdong Liu Ying Liu Juan Tang Xinyun Li Qigai He Shuhong Zhao | 2010 | Molecular Immunology2010，，7： | 1 |
| 6 | The co-administrating of recombinant porcine IL-2 could enhance protective immune responses to PRV inactivated vaccine in pigs显示文摘 | Lin Y Qigai H Xiaolan Y | 2005 | Vaccine2005，23，35： | 1 |
| 7 | Occurrence and investigation of enteric viral infections in pigs with diarrhea in China显示文摘 | Qian Zhang Ruiming Hu Xibiao Tang Chenglong Wu Qigai He Zhanqin Zhao Huanchun Chen Bin Wu | 2013 | Archives of Virology2013，，： | 1 |
| 8 | The co-administrating of recombinant porcine IL-2 could enhance protective immune responses to PRV inactivated vaccine in pigs显示文摘 | Lin Y Qigai H Xiaolan Y | 2005 | Vaccine2005，23，35： | 1 |
| 9 | The co-administrating of recombinant porcine IL-2 could enhance protective immune responses to PRV inactivated vaccine in pigs显示文摘 | Lin Y Qigai H Xiaolan Y Weicheng B | 2005 | Vaccine2005，23，35： | 1 |
| 10 | Broad activity against porcine bacterial pathogens displayed by two insect antimicrobial peptides moricin and cecropin B显示文摘 | Han Hu Chunmei Wang Xiaozhen Guo Wentao Li Yang Wang Qigai He | 2013 | Molecules and Cells2013，，2： | 1 |
| 11 | The co-administrating of recombinant porcine IL-2 could enhance protective immune responses to PRV inactivated vaccine in pigs显示文摘 | Lin Y Qigai H Xiaolan Y | | 0，，35： | 1 |
| 12 | Construction and characterization of a live, attenuated apxIICA inactivation mutant of Actinobacillus pleuropneumoniae lacking a drug resistance marker显示文摘 | Weicheng Bei Qigai He Lin Yan Liurong Fang Yadi Tan Shaobo Xiao Rui Zhou Meilin Jin Aizhen Guo Jianqiang Lv Hongliang Huang Huanchun Chen | 2004 | FEMS Microbiology Letters2004，，1： | 1 |
| 13 | Occurrence and investigation of enteric viral infections in pigs with diarrhea in China显示文摘 | Qian Zhang Ruiming Hu Xibiao Tang Chenglong Wu Qigai He Zhanqin Zhao Huanchun Chen Bin Wu | 2013 | Archives of Virology2013，，8： | 1 |
| 14 | The co-administrating of recombinant porcine IL-2 could enhance protective immune responses to PRV inactivated vaccine in pigs显示文摘 | Yan Lin He Qigai Yu Xiaolan Bei Weicheng Chen Huanchun | 2005 | Vaccine2005，，35： | 1 |
| 15 | The development and the application of latex agglutination test to detect serum antibodies against Japanese encephalitis virus 显示文摘 | Xinglin J Huanchun C Qigai H | | Vet Res Commun0，26，6： | 1 |
| 16 | Broad activity against porcine bacterial pathogens displayed by two insect antimicrobial peptides moricin and cecropin B显示文摘 | Han Hu Chunmei Wang Xiaozhen Guo Wentao Li Yang Wang Qigai He | 2013 | Molecules and Cells2013，，2： | 1 |
| 17 | Development of an indirect immunofluorescence assay for PCV3 antibody detection based on capsid protein显示文摘Porcine circovirus type 3(PCV3)is a novel porcine circovirus associated with porcine dermatitis and nephritis syndrome(PDNS),reproductive failure,and multisystemic inflammation.Capsid protein(Cap)encoded by PCV3 ORF2 gene has been identified as an immunogenic protein.Currently,there is no immunofluorescence assay(IFA)available for serological diagnosis.Here,the N-terminal 33 amino acids of Cap protein were predicted to serve as a PCV3 nuclear localization signal(NLS).Two types of recombinant plasmids were constructed for recombinant protein expression in 5f9 cells by using a baculovirus expression system:plasmid rvBac-Pc for full-length Cap protein expression and rvBac-Sc for Cap protein expression with a honeybee melittin signal peptide in place of the predicted NLS sequence.Expression of the nuclear localization sequences was further analyzed by IFA.Strong and specific fluorescence signals were observed in the nucleus of rvBac-Pc-transfected cells and in the cytoplasm of rvBac-Sc-transfected cells.No cross-reactivity was observed with porcine circovirus type 2,porcine pseudorabies virus,classical swine fever virus,or porcine reproductive and respiratory syndrome virus.In summary,we developed two fluorescence detection modes for Cap protein that can be used to detect PCV3 antibodies.This method is suitable for the diagnosis and epidemiological investigation of PCV3.This study provides a reliable detection method for monitoring PCV3 antibody level in pigs in the future. | Lun Yao Chang Li Junwei Wang Yufang Cheng Ahmed H.Ghonaim Qi Sun Xuexiang Yu Weijie Niu Shengxian Fan Qigai He | 2021 | Animal Diseases2021，1，2： | 0 |
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