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| 1 | Ellagic acid induces apoptosis through inhibition of nuclear factor κB in pancreatic cancer cells显示文摘AIM:To determine the effect of ellagic acid on apop-tosis and proliferation in pancreatic cancer cells and to determine the mechanism of the pro-survival effects of ellagic acid. METHODS:The effect of ellagic acid on apoptosis was assessed by measuring Phosphatidylserine externalization,caspase activity,mitochondrial membrane potential and DNA fragmentation;and proliferation by measuring DNA thymidine incorporation. Mitochondrial membrane potential was measured in permeabilized cells,and in isolated mitochondria. Nuclear factor kB(NF-kB) activity was measured by electromobility shift assay(EMSA) . RESULTS:We show that ellagic acid,a polyphenolic compound in fruits and berries,at concentrations 10 to 50 mmol/L stimulates apoptosis in human pancreatic adenocarcinoma cells. Further,ellagic acid decreases proliferation by up to 20-fold at 50 mmol/L. Ellagic acid stimulates the mitochondrial pathway of apoptosis associated with mitochondrial depolarization,cytochrome C release,and the downstream caspaseactivation. Ellagic acid does not directly affect mitochondria. Ellagic acid dose-dependently decreased NF-kB binding activity. Furthermore,inhibition of NF-kB activity using IkB wild type plasmid prevented the effect of ellagic acid on apoptosis. CONCLUSION:Our data indicate that ellagic acid stimulates apoptosis through inhibition of the prosu-rvival transcription factor NF-kB. | Mouad Edderkaoui Irina Odinokova Izumi Ohno Ilya Gukovsky Vay Liang W Go Stephen J Pandol Anna S Gukovskaya | 2008 | World Journal of Gastroenterology2008，14，23： | 30 |
| 2 | A Starring Role for Stellate Cells in the Pancreatic Cancer Microenvironment显示文摘 | Minoti V. Apte Jeremy S. Wilson Aurelia Lugea Stephen J. Pandol | 2013 | Gastroenterology2013，，6： | 6 |
| 3 | XIAP抑制剂Embelin体外对人胰腺癌细胞株的抑制效应与机制研究显示文摘目的探讨XIAP小分子抑制剂Embelin体外对人胰腺癌细胞株MIAPaCa-2的抑制效应与机制。方法将生长至第4-12代之间的MIAPaca-2细胞分为DMSO对照组与Embelin实验组,在实验组细胞中加入不同浓度的Embelin共孵育72h,收集细胞并裂解后取上清液用细胞凋亡试剂盒检测组蛋白(凋亡指标)的OD值水平。MIAPaca-2细胞加入不同浓度的Embelin共孵育60h后再加入3H胸腺嘧啶继续孵育12h,取细胞裂解液用同位素γ闪烁计数仪检测3H-CPM数值以判断细胞增殖程度。对DMSO对照组与Embelin实验组的组蛋白OD值与3H-CPM数值分别进行比较分析。结果Embelin实验组的MIAPaCa-2细胞株组蛋白OD值显著高于DMSO对照组而3H-CPM数值明显低于DMSO对照组(P<0.01),且实验组Embe-lin的作用浓度与组蛋白OD值呈正相关(r=0.996,P<0.01),而与3H-CPM数值呈负相关(r=-0.993,P<0.01)。结论Embelin呈剂量依赖性地诱导人胰腺癌细胞株MIAPaCa-2凋亡并抑制其增殖,有肿瘤抑制效应。 | 郑玲 吕寒静 Mouad Edderkaoui Stephen J Pandol | 2009 | 山西医科大学学报2009，40，1： | 4 |
| 4 | Alcoholic hepatitis: The pivotal role of Kupffer cells显示文摘Kupffer cells play a central role in the pathogenesis of alcoholic hepatitis(AH). It is believed that alcohol increases the gut permeability that results in raised levels of serum endotoxins containing lipopolysaccharides(LPS). LPS binds to LPS-binding proteins and presents it to a membrane glycoprotein called CD14, which then activates Kupffer cells via a receptor called tolllike receptor 4. This endotoxin mediated activation of Kupffer cells plays an important role in the inflammatory process resulting in alcoholic hepatitis. There is no effective treatment for AH, although notable progress has been made over the last decade in understanding the underlying mechanism of alcoholic hepatitis. We specifically review the current research on the role of Kupffer cells in the pathogenesis of AH and the treatment strategies. We suggest that the imbalance between the pro-inflammatory and the anti-inflammatory process as well as the increased production of reactive oxygen species eventually lead to hepatocyte injury, the final event of alcoholic hepatitis. | Duminda B Suraweera Ashley N Weeratunga Robert W Hu Stephen J Pandol Richard Hu | 2015 | World Journal of Gastrointestinal Pathophysiology2015，6，4： | 3 |
| 5 | Emerging concepts for the mechanism of alcoholic pancreatitis from experimental models显示文摘 | Stephen J. Pandol Ilya Gukovsky Akihiko Satoh Aurelia Lugea Anna S. Gukovskaya | 2003 | Journal of Gastroenterology2003，，7： | 2 |
| 6 | Acute Pancreatitis: Bench to the Bedside显示文摘 | Stephen J. Pandol Ashok K. Saluja Clement W. Imrie Peter A. Banks | 2007 | Gastroenterology2007，，3： | 2 |
| 7 | Substance P mediates cerulein -inducedpancreatic microcirculatory dysfunction in mice 显示文摘 | Ito Y Lugea A Pandol SJ | 2007 | Pancreas2007，34，1： | 1 |
| 8 | Acute pancreatitis: bench to the bedside 显示文摘 | Pandol SJ Saluja AK Imrie CW | 2007 | Gastroenterology2007，132，3： | 1 |
| 9 | Grp78 Heterozygosity Regulates Chaperone Balance in Exocrine Pancreas with Differential Response to Cerulein-Induced Acute Pancreatitis显示文摘 | Risheng Ye Olga A. Mareninova Ernesto Barron Miao Wang David R. Hinton Stephen J. Pandol Amy S. Lee | 2010 | The American Journal of Pathology2010，，6： | 1 |
| 10 | Ethanol dietincreases the sensitivith of rats to pancreatitis induced by cholecystokinin oc- tapeptide显示文摘 | Pandol ST Periskic S Gukovsky I | 1999 | Gastrenterology1999，117，： | 1 |
| 11 | Reactive oxygen species produced by NAD(P)H oxidase inhibit apoptosis in pancreatic cancer cells显示文摘 | Vaquero EC Edderkaoui M Pandol SJ Gukovasky I | 2004 | J Biol Chem2004，279，34： | 1 |
| 12 | Mitochondrial injury in pancreatitis显示文摘 | R. Mukherjee D.N. Criddle A. Gukvoskaya S. Pandol O.H. Petersen R. Sutton | 2007 | Cell Calcium2007，，1： | 1 |
| 13 | Acute Pancreatitis: Bench to the Bedside显示文摘 | Stephen J. Pandol Ashok K. Saluja Clement W. Imrie Peter A. Banks | 2007 | Gastroenterology2007，，3： | 1 |
| 14 | Ethanol diet increases the sensitivity of rats to pancreatitis induced by cholecystokinin octapeptide显示文摘 | Pandol ST Periskic S Gukovsky I | | 0，，： | 1 |
| 15 | Reactive oxygen species produced by NAD(P)H oxidase inhibit apoptosis in pancreatic cancer cells显示文摘 | Vaquero EC Edderkaoui M Pandol SJ | 2004 | J Biol Chem2004，279，34： | 1 |
| 16 | Substance P mediates cerulein-induced panceatic microciculatory dysfunction in mice显示文摘 | Ito Y Lugea A Pandol S J | 2007 | Pancreas2007，34，1： | 1 |
| 17 | Acute pancreatitis 显示文摘 | Pandol SJ | 2005 | Curr Opiu Gastroenterol2005，21，10： | 1 |
| 18 | Mechanisms of cell death after pancreatic duct obstruction in the opossum and the rat显示文摘 | AS Gukovskaya P Perkins V Zaninovic D Sandoval R Rutherford T Fitzsimmons SJ Pandol S Poucell-Hatton | 1996 | Gastroenterology1996，，3： | 1 |
| 19 | Parathyroid hormone- related protein expression in human gastric adenocarcinomas not associated with hypercalcemia显示文摘 | Abdeen O Pandol SJ Burton DW | 1995 | Am J Gastroenterol1995，90，10： | 1 |
| 20 | Acute pancreatitis:bench to the bedside显示文摘 | Pandol SJ Saluja AK lmrie CW | | 0，，03： | 1 |
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