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| 1 | Hippo/Yap signaling controls epithelial progenitor cell proliferation and differentiation in the embryonic and adult lung显示文摘The Hippo/Yap pathwayis a wll-conserved signaling cascade that regulates cell proliferation and dfferentiationto control organsize and stem/progenitor cell behavior.Following airway injury,Yap was dynamically regulated in regenerating airway epithelial clls.To deter.mine the role ofHippo signaling in the lung,the mammalian Hippo kinases,Mst1 and Mst2,were deleted in epithelial cells ofthe embry-onic and mature mouse lung.Mst1/2 deletion in the fetal lung enhanced proliferation and inhibited sacculation and epithelial cell differentiation.The transcriptional inhibition of cell proliferation and activation of differentiation during normal perinatal lung maturation were inversely regulated followving embryonic Mst1/2 deletion.Ablation of Mst1/2 from bronchiolar epithelial cells in the adult lung caused airway hyperplasia and altered differentiation.Inhibitory Yap phosphorylation was decreased and Yap nuclear localization and transcriptional targets were increased after Mst1/2 deletion,consistent with canonical Hippo/Yap signaling.YAP potentiated cell prolif-eration and inhibited dfferentiation of human bronchial epithelial cells in vitro.Loss of Mst1/2 and expression ofYAP regulated transcrip-tional targets controlling cell proliferation and diferentiation,including Ajuba LIM protein.Ajuba was required forthe effects ofYAP oncell proliferation in vitro.Hippo/Yapsignaling regulates Ajuba and controls proliferation and diferentiation oflung epithelial progenitor cells. | Alexander W.Lange Anusha Sridharan Yan Xu Barry R.Stripp Anne-Karina Perl Jeffrey A.Whitsett | 2015 | Journal of Molecular Cell Biology2015，7，1： | 4 |
| 2 | 欧洲和中国的心脏与肾脏远程缺血预适应研究:一项随机对照试验显示文摘远程缺血预适应(remote ischemic preconditioning,RIPC)对经皮冠状动脉介入治疗(percutaneous coronary intervention,PCI)术后造影剂肾病(contrast-induced nephropathy,CIN)的潜在保护作用尚待确定。该研究为双盲、随机、安慰剂对照的多中心研究,纳入年龄〈85岁,肾清除率为30~60mL/(min·1.73m^2)的有临床适应证的患者,除了直接PCI外均按1∶1给予RIPC或标准治疗。主要终点是CIN的发生率。次要终点是围手术期心肌梗死(peri-procedural myocardial infarction,PMI)的发生率。 | 刘青 叶鹏 Moretti C Cerrato E Cavallero E Lin S Rossi ML Picchi A Sanguineti F Ugo F Palazzuoli A Bertaina M Presbitero P Shao-Liang C Pozzi R Giammaria M Limbruno U Lefèvre T Gasparetto V Garbo R OmedèP Sheiban I Escaned J Biondi-Zoccai G Gaita F Perl L D'Ascenzo F | 2018 | 中华高血压杂志2018，26，4： | 3 |
| 3 | Cloning and Characterization of WOX4 Gene from Vitis vinifera L. Involved in Stem Cell Regulation显示文摘Wuschel-related homeobox (WOX) genes play essential, specific, and sometimes redundant roles in plant embryo development, shoot and root meristem maintenance, and plant development. Though much information was quickly gained with members of the WOX gene family of Arabidopsis, monocotyledonous crops, and gymnospermous conifers, little is known about perennial woody plants. In this study, we isolated the first WOX gene family member from grape (Vitis vinifera L. cv. Cabernet Sauvignon), and named it VvWOX4 based on its characteristic domains and phylogenetic analysis. The identity of VvWOX4 was validated by MALDI-TOF MS and Western blot with polyclonal antibody against Arabidopsis thaliana Wuschel. Functional analysis showed that VvWOX4 markedly increased shoot primordia structures when overexpressed under CaMV 35S promoter in tobacco. A different expression pattern was found for VvVOX4 compared with AtWUCHEL and its expression was detected in unique organs of grapevines. Besides the expression in the vegetative shoot apical meristem (SAM) of grape shoot tips, VvWOX4 is expressed in dormant winter buds, inflorescence, young leaves, and tendril tips, but not in root tips. In young leaves, the expression of VvWOX4 is strongly upregulated by wounding, and also by plant growth regulators such as 2 mg L-1 2,4-D, 1 mg L-1 NAA and 1 mg L-1 BAP treatments, while downregulation was monitored by 1 mg L-1 IBA treatment, and there was no response to 0.5 mg L-1 GA3 treatment. Together, our results revealed the first member of grape WOX gene family and indicated different roles and regulation of VvWOX4 in the perennial woody crop grapevine. | DAI Ru JIN Hai-peng WANG Zeng Avihai Perl XU Hai-ying ZHANG Wen CHEN Shang-wu MA Hui-qin | 2011 | Agricultural Sciences in China2011，10，12： | 3 |
| 4 | The Common Feature of Leukemia-Associated IDH1 and IDH2 Mutations Is a Neomorphic Enzyme Activity Converting α-Ketoglutarate to 2-Hydroxyglutarate显示文摘 | Patrick S. Ward Jay Patel David R. Wise Omar Abdel-Wahab Bryson D. Bennett Hilary A. Coller Justin R. Cross Valeria R. Fantin Cyrus V. Hedvat Alexander E. Perl Joshua D. Rabinowitz Martin Carroll Shinsan M. Su Kim A. Sharp Ross L. Levine Craig B. Thompson | 2010 | Cancer Cell2010，，3： | 2 |
| 5 | The Establishment of an Agrobacterium-Mediated Transformation Platform for the Non-Embryogenic Calli of Vitis vinifera L.显示文摘Non-embryogenic calli (NEC) was inevitably and heavily produced when grape embryogenic calli (EC) was induced from explants or during the subculture of EC.A stable and highly efficient NEC transformation platform is required to further sort out and verify key genes which determine/switch the identity of NEC and EC.In this research,a vector pA5 containing a chitinase signal sequence fused to gfp (green fluorescent protein) and an HDEL motive was used to target and immobilize into Agrobacterium strain EHA105 to establish a transformation platform for Vitis vinifera L.cv.Chardonnay NEC.It was determined that NEC 10 d after subculture was the best target tissue;30 min for inoculation followed by 3 d co-cultivation with the addition of 200 μmol L-1 acetosyringone (AS) was optimized as protocol.The use of bacterial densities as 1.0 at OD600 did not result in serious tissue hypersensitive reaction and it had higher efficiency.Kanamycin at 200 mg L-1 was picked for positive expression selection.The stable transformation of NEC was proved by reverse transcription-polymerase chain reaction techniques (RT-PCR) and fluorescent microscopy after three sub-cultures of the selected cell line.Highly efficient genetic transformation protocol of grape NEC was achieved and some of the optimized parameters were different from that reported for EC.This transformation platform could facilitate the verification of candidate somatic embryogenesis (SE) decisive genes,and the successfully transformed NEC with certain genes can also be used as bioreactors for the production of functional products,as NEC not only proliferates fast,but also keeps in a rather stable condition. | ZHAO Feng-xia CHEN Shang-wu Perl Avihai DAI Ru XU Hai-ying MA Hui-qin | 2011 | Agricultural Sciences in China2011，10，5： | 2 |
| 6 | Intracardiac Echogenic Foci Have No Hemodynamic Significance in the Fetus显示文摘 | Zeev Perles Amiram Nir Sagui Gavri Julius Golender Azaria J. J. T. Rein | 2010 | Pediatric Cardiology2010，，1： | 2 |
| 7 | Comparison of narrow band (311nm) UVB and UVA after oral or bath water 8-methoxy poralen in the treatment of poriasis显示文摘 | Orteo B Perl S Kinaciyan T | 1993 | J Am Acad Dermatol1993，29，51： | 1 |
| 8 | A causal role for E-cadherin in the transition from adenoma to carcinoma显示文摘 | PERL A K WILGENBUS P DAHL U | 1998 | Nature1998，392，6672： | 1 |
| 9 | Improvement of plant regeneration and GUS expression in scutellar wheat calli by optimization of culture conditions and DNA-microprojectile delivery procedures显示文摘 | Perl A Kless H Galili G | 1992 | Molecular and General Genetics1992，235，23： | 1 |
| 10 | Pulmonary contusion induces alveolar type 2 epithelial cell apoptosis: role of alveolar macrophages and neutro- phils显示文摘 | Seitz DH Perl M Marigold S | 2008 | Shock2008，30，5： | 1 |
| 11 | NT-pro-BNP as a marker for persistent cardiac disease in children with history of dilated cardiomyopathy and myocarditis显示文摘 | Nasser N Perles Z Rein A J | 2006 | Pediatr Cardiol2006，27，： | 1 |
| 12 | A causal role for E-cadherin in the transition from adenoma to carcinoma显示文摘 | Perl AK Wilgenbus P Dahl U | 1998 | Nature1998，392，11： | 1 |
| 13 | Cardiomyogenesis in the adult human heart显示文摘 | Kajstura J Urbanek K Perl S | 2010 | Circ Res2010，107，2： | 1 |
| 14 | Epithelial cell apoptosis and neutrophil recruitment in acute lung injury- a unifying hypothesis? What we have learned from small interfering rnas 显示文摘 | Perl M Lomas-Neira J Chung C S | 2008 | Mol Med2008，14，78： | 1 |
| 15 | The complexity of finding maximum disjoint paths with length constraints 显示文摘 | Itai A Perl Y Shiloah Y | 1982 | Networks1982，，12： | 1 |
| 16 | Acinetobacter baumannii: epidemiology, antimicrobial resistance,and treatment options 显示文摘 | Maragakis L L Perl T M | 2008 | Clin Infect Dis2008，46，12： | 1 |
| 17 | Group contribution analysis of the damping behavior of homopolymers,statistical copolymers and interpenetrating polymer networks based on acrylic,vinyl and styrenicmers显示文摘 | CHANG M C O THOMAS D PERLING L H | | 0，，： | 1 |
| 18 | Hemodialysis vascular access modifies the association between dialysis modality and survival显示文摘 | Perl J Wald R McFarlane P | | 0，，06： | 1 |
| 19 | Dextran sodium sulphate colitis mouse model: traps and tricks 显示文摘 | Perle M Cerar A | 2012 | J Biomed Biotechnol2012，，71： | 1 |
| 20 | A causal role for E-cadherin in the transition from adenoma to carcinoma显示文摘 | Perl AK Wilgenbus P Dahl U | 1998 | Nature1998，392，6672： | 1 |
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