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| 1 | Transplantation of UC-MSCs on collagen scaffold activates follicles in dormant ovaries of POF patients with long history of infertility显示文摘Premature ovarian failure(POF) is a refractory disease for clinical treatment with the goal of restoring fertility. In this study,umbilical cord mesenchymal stem cells on a collagen scaffold(collagen/UC-MSCs) can activate primordial follicles in vitro via phosphorylation of FOXO3 a and FOXO1. Transplantation of collagen/UC-MSCs to the ovaries of POF patients rescued overall ovarian function, evidenced by elevated estradiol concentrations, improved follicular development, and increased number of antral follicles. Successful clinical pregnancy was achieved in women with POF after transplantation of collagen/UC-MSCs or UC-MSCs. In summary, collagen/UC-MSC transplantation may provide an effective treatment for POF. | Lijun Ding Guijun Yan Bin Wang Lu Xu Yan Gu Tong Ru Xiaoying Cui Lei Lei Jingyu Liu Xiaoqiang Sheng Bin Wang Chunxue Zhang Yanjun Yang Ruiwei Jiang Jianjun Zhou Na Kong Feifei Lu Huaijun Zhou Yannan Zhao Bing Chen Yali Hu Jianwu Dai Haixiang Sun | 2018 | Science China(Life Sciences)2018，61，12： | 43 |
| 2 | Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman's syndrome显示文摘Asherman's syndrome(AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells(BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS. | Guangfeng Zhao Yun Cao Xianghong Zhu Xiaoqiu Tang Lijun Ding Haixiang Sun Juan Li Xinan Li Chenyan Dai Tong Ru Hui Zhu Jingjie Lu Caimei Lin Jingmei Wang Guijun Yan Huiyan Wang Lei Wang Yimin Dai Bin Wang Ruotian Li Jianwu Dai Yan Zhou Yali Hu | 2017 | Science China(Life Sciences)2017，60，4： | 39 |
| 3 | Assessment of duodenogastric reflux by combined continuous intragastric pH and bilirubin monitoring显示文摘AIM: To assess the diagnostic value of a combination of continuous intragastric pH and bilirubin monitoring in the detection of duodenogastric reflux (DGR), and the effects of diet on the bilirubin absorbance.METHODS: 30 healthy volunteers were divided into twogroups: standard diet group (Group 1) 18 cases, free diet group (Group 2) 12 cases. Each subjects were subjected to simultaneous 24 hour intragastric pH and spectrophotometric bilirubin concentration monitoring (Bilitec 2000).RESULTS: There was no difference of preprandial phasebilirubin absorbance between two groups. The absorbanceof postprandial phase was significantly increased in group 2than group 1. There was no difference between preprandialphase and postprandial phase absorbance in group 1.Postprandial phase absorbance was significantly higher ingroup 2. In a comparison of bile reflux with intragastric pHduring night time, there were 4 types of reflux:Simultaneous increase in absorbance and pH in only 19.6%, increase in bilirubin with unchanged pH 33. 3 %, pHincrease with unchanged absorbance 36. 3 %, and bothunchanged in 10. 8 %. Linear regression analysis showed nocorrelation between percertage total time of pH < 4 aridpercentage total time of absortance > 0. 14, r=0.068, P<0.05.CONCLUSION: Because of the dietary effect, highabsorbance fluids or foods should be avoided in detection.Intrsgastric pH and bilirubin monitoring separately predictthe presence of duodenal (and/or pancreatic) reflux and bilereflux. They can not substitute for each other. The detectionof DGR is improved if the two parameters are combinedsimultanoously. | Fei Dai Jun Gong Ru Zhang Jin-Yan Luo You-Ling Zhu Xue-Qin Wang,Department of Gastroenterology,Second Hospital of Xi’ an Jiaotong University,Xi’an 710004,Shaanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 30 |
| 4 | Collagen-binding basic fibroblast growth factor improves functional remodeling of scarred endometrium in uterine infertile women: a pilot study显示文摘Intrauterine adhesion(IUA) is a common cause of uterine infertility and one of the most severe clinical features is endometrial fibrosis namely endometrial scarring for which there are few cures currently. Blocked angiogenesis is the main pathological change in the scarred endometrium. The fibroblast growth factor 2(b FGF), a member of FGF family, is usually applied to promote healing of refractory ulcer and contributes to angiogenesis of tissues. In this study, the sustained-release system of b FGF100 μg was administrated around scarred endometrium guiding by ultrasound every 4 weeks in 18 patients(2–4 times). Results showed that after treatment, the menstrual blood volume, endometrial thickness and the scarred endometrial area were improved.Histological study showed blood vessel density increased obviously. Three patients(3/18) achieved pregnancy over 20 gestational weeks. Therefore, administrating the b FGF surrounding scarred endometrium may provide a new therapeutic approach for the patients with endometrial fibrosis. | Peipei Jiang Xiaoqiu Tang Huiyan Wang Chenyan Dai Jing Su Hui Zhu Minmin Song Jingyu Liu Ziqing Nan Tong Ru Yaling Li Jingmei Wang Jun Yang Bing Chen Jianwu Dai Yali Hu | 2019 | Science China(Life Sciences)2019，62，12： | 16 |
| 5 | Relaxant Effects of Matrine on Aortic Smooth Muscles of Guinea Pigs显示文摘Objective To determine whether matrine, a kind of traditional Chinese medicinal alkaloid, can relax the aortic smooth muscles isolated from guinea pigs and to investigate the mechanism of its relaxant effects. Methods Phenylephrine or potassium chloride concentration-dependent relaxation response of aortic smooth muscles to matrine was studied in the precontracted guinea pigs. Results Matrine (1×10-4 mol/L -3.3×10-3 mol/L) relaxed the endothelium-denuded aortic rings pre-contracted sub-maximally with phenylephrine, in a concentration-dependent manner, and its pre-incubation (3.3×10-3 mol/L) produced a significant rightward shift in the phenylephrine dose-response curve, but had no effects on the potassium chloride-induced contraction. The anti-contractile effect of matrine was not reduced by the highly selective ATP-dependent K+ channel blocker glibenclamide (10-5 mol/L), either by the non-selective K+ channel blocker tetraethylammonium (10-3 mol/L), or by the β-antagonist propranolol (10-5 mol/L). In either 'normal' or 'Ca2+-free' bathing medium, the phenylephrine-induced contraction was attenuated by matrine (3.3×10-3 mol/L), indicating that the vasorelaxation was due to inhibition of intracellular and extracellular Ca2+ mobilization. Conclusion Matrine inhibits phenylephrine-induced contractions by inhibiting activation of α-adrenoceptor and interfering with the release of intracellular Ca2+ and the influx of extracellular Ca2+. | JIE ZHENG PING ZHENG XU ZHOU LIN YAN RU ZHOU XUE-YAN FU GUI-DONG DAI | 2009 | Biomedical and Environmental Sciences2009，22，4： | 6 |
| 6 | Association between Metabolic Syndrome and Carotid Atherosclerosis: A Cross-sectional Study in Northern China显示文摘Objective Increased carotid artery intima-media thickness(CIMT)and carotid plaque as manifestations of carotid atherosclerosis have been used as markers of cardiovascular disease(CVD).The components of metabolic syndrome(Met S)are linked to CVD,but the association between Met S and CVD is controversial.Methods A total of 8,933 Chinese adults aged 40 years or older from 2010 to 2014 were selected from the Jidong and Kailuan communities.Met S was defined by the International Diabetes Federation criteria.CIMT and carotid plaque were measured using color Doppler ultrasound.Logistic regression models were used to assess the association of Met S with carotid plaque and CIMT.Results Met S was found among 3,461(3,461/8,933)participants.The odds ratio and 95%confidence internal(CI)for carotid plaques in participants with Met S was 1.16(1.03-1.30).The risk of carotid plaques increased with the number of Met S components.The average CIMT was higher in participants with Met S(β=0.020,95%CI,0.014-0.027)and in participants with more Met S components.Conclusion Individuals with Met S are at an increased risk for carotid atherosclerosis compared to those without MetS. | ZHOU Ping An ZHANG Chen Huan CHEN Yan Ru LI Dong SONG Dai Yu LIU Hua Min ZHOU Ming Yue SONG Guo Shun CHEN Sheng Yun | 2019 | Biomedical and Environmental Sciences2019，32，12： | 6 |
| 7 | Characterization and adsorption behavior of a novel triolein-embedded activated carbon composite adsorbent显示文摘A novel triolein-embedded activated carbon composite adsorbent was developed. Experiments were car-ried out in areas such as the preparation method, the char-acterization of physicochemical properties, and the adsorp-tion behavior of the composite adsorbent in removing diel-drin from aqueous solution. Results suggested that the novel composite adsorbent was composed of the supporting acti-vated carbon and the surrounding triolein-embedded cellu-lose acetate membrane. The adsorbent was stable in water, for no triolein leakage was detected after soaking the ad-sorbent for five weeks. The adsorbent had good adsorption capability to dieldrin, which was indicated by a residual di-eldrin concentration of 0.204 μg·L?1. The removal efficiency of the composite adsorbent was higher than the traditional activated carbon adsorbent. | RU Jia LIU Huijuan QU Jiuhui WANG Aimin DAI Ruihua | 2005 | Chinese Science Bulletin2005，50，23： | 6 |
| 8 | Gender differences are associated with the clinical features of systemic lupus erythematosus显示文摘 | DING Yan HE Jing GUO Jian-ping DAI Yi-jun LI Chun FENG Min LI Ru LI Zhan-guo | 2012 | Chinese Medical Journal2012，，14： | 5 |
| 9 | Cloning and Characterization of WOX4 Gene from Vitis vinifera L. Involved in Stem Cell Regulation显示文摘Wuschel-related homeobox (WOX) genes play essential, specific, and sometimes redundant roles in plant embryo development, shoot and root meristem maintenance, and plant development. Though much information was quickly gained with members of the WOX gene family of Arabidopsis, monocotyledonous crops, and gymnospermous conifers, little is known about perennial woody plants. In this study, we isolated the first WOX gene family member from grape (Vitis vinifera L. cv. Cabernet Sauvignon), and named it VvWOX4 based on its characteristic domains and phylogenetic analysis. The identity of VvWOX4 was validated by MALDI-TOF MS and Western blot with polyclonal antibody against Arabidopsis thaliana Wuschel. Functional analysis showed that VvWOX4 markedly increased shoot primordia structures when overexpressed under CaMV 35S promoter in tobacco. A different expression pattern was found for VvVOX4 compared with AtWUCHEL and its expression was detected in unique organs of grapevines. Besides the expression in the vegetative shoot apical meristem (SAM) of grape shoot tips, VvWOX4 is expressed in dormant winter buds, inflorescence, young leaves, and tendril tips, but not in root tips. In young leaves, the expression of VvWOX4 is strongly upregulated by wounding, and also by plant growth regulators such as 2 mg L-1 2,4-D, 1 mg L-1 NAA and 1 mg L-1 BAP treatments, while downregulation was monitored by 1 mg L-1 IBA treatment, and there was no response to 0.5 mg L-1 GA3 treatment. Together, our results revealed the first member of grape WOX gene family and indicated different roles and regulation of VvWOX4 in the perennial woody crop grapevine. | DAI Ru JIN Hai-peng WANG Zeng Avihai Perl XU Hai-ying ZHANG Wen CHEN Shang-wu MA Hui-qin | 2011 | Agricultural Sciences in China2011，10，12： | 3 |
| 10 | Preparation and characterization of a novel adsorbent for removing lipophilic organic from water显示文摘A novel composite adsorbent containing a kind of lipid-triolein is studied. The ad-sorbent is prepared by embedding triolein into cellulose acetate (CA) sphere. The preparation method, the physical-chemical properties of the adsorbent and the removal efficiency of two or-ganochlorinated pesticides are studied. The adsorbent is stable in water and no triolein leaks into water for 465 h soaking. The adsorbent has high adsorption capacity for organochlorinated pes-ticides such as dieldrin and aldrin. The results suggest that triolein-containing adsorbent could serve as a good adsorbent for lipophilic organic pollutants. The adsorption rate for lipophilic pol-lutants is very fast and has relation with the logKow of the compounds. | LIU Huijuan1, DAI Ruihua1, QU Jiuhui1 & RU Jia1,2 1. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China | 2005 | Science China Chemistry2005，48，6： | 3 |
| 11 | A Simple Approach to Control the Growth of Non-spherical Gold Nanoparticles显示文摘A simple method to prepare the non-spherical gold particles was developed. The result solution included trigonal, truncated trigonal, hexagonal layers, and a pseudo-pentagonal shaped gold nanocrystals. The key factor is to control the relative rates of nucleation and cluster growth in this method. These attributes make seeding growth method as a useful tool in the fabrication of colloidal metal materials. A longitudinal plasmon resonance of 866 nm was observed, which is in the near-IR spectral regions (600-1000 nm). The excellent optical properties as near-IR labels are used to develop highly sensitive analysis method. | Hong YUAN, Ru Xiu CAI*, Dai Wen PANG College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 | 2003 | Chinese Chemical Letters2003，14，11： | 2 |
| 12 | Hydrothermal Synthesis and Characterization of Perovskite PbTiO_3 Powders from Different Precursors显示文摘IntroductionPerovskite(PE)phasePbTiO3isawel-knownferroelectricmaterialwithexcelentdi-electric,pyroelectricandpiezoelectricpro... | CHEN Dai rong and XU Ru ren ** (Department of Chemistry, Jilin University, Changchun, 130023) | 1998 | Chemical Research in Chinese Universities1998，14，3： | 2 |
| 13 | The Establishment of an Agrobacterium-Mediated Transformation Platform for the Non-Embryogenic Calli of Vitis vinifera L.显示文摘Non-embryogenic calli (NEC) was inevitably and heavily produced when grape embryogenic calli (EC) was induced from explants or during the subculture of EC.A stable and highly efficient NEC transformation platform is required to further sort out and verify key genes which determine/switch the identity of NEC and EC.In this research,a vector pA5 containing a chitinase signal sequence fused to gfp (green fluorescent protein) and an HDEL motive was used to target and immobilize into Agrobacterium strain EHA105 to establish a transformation platform for Vitis vinifera L.cv.Chardonnay NEC.It was determined that NEC 10 d after subculture was the best target tissue;30 min for inoculation followed by 3 d co-cultivation with the addition of 200 μmol L-1 acetosyringone (AS) was optimized as protocol.The use of bacterial densities as 1.0 at OD600 did not result in serious tissue hypersensitive reaction and it had higher efficiency.Kanamycin at 200 mg L-1 was picked for positive expression selection.The stable transformation of NEC was proved by reverse transcription-polymerase chain reaction techniques (RT-PCR) and fluorescent microscopy after three sub-cultures of the selected cell line.Highly efficient genetic transformation protocol of grape NEC was achieved and some of the optimized parameters were different from that reported for EC.This transformation platform could facilitate the verification of candidate somatic embryogenesis (SE) decisive genes,and the successfully transformed NEC with certain genes can also be used as bioreactors for the production of functional products,as NEC not only proliferates fast,but also keeps in a rather stable condition. | ZHAO Feng-xia CHEN Shang-wu Perl Avihai DAI Ru XU Hai-ying MA Hui-qin | 2011 | Agricultural Sciences in China2011，10，5： | 2 |
| 14 | MimoDB:a new repositoryfor mimotope data derived from phage display technology 显示文摘 | RU Beibei HUANG Jian DAI Ping | 2010 | Molecules2010，15，11： | 1 |
| 15 | Dexmedetomidine reduces the incidence of fentanyl - induced colxgh: a double -blind, randomized, and placebo- controlled study 显示文摘 | HE Liang XU Jun - mei DAI Ru - ping | 2012 | Ups J Med Sci2012，117，1： | 1 |
| 16 | miR‐148a regulates osteoclastogenesis by targeting V‐maf musculoaponeurotic fibrosarcoma oncogene homolog B显示文摘 | Peng Cheng Chao Chen Hong‐Bo He Rong Hu Hou‐De Zhou Hui Xie Wu Zhu Ru‐Chun Dai Xian‐Ping Wu Er‐Yuan Liao Xiang‐Hang Luo | 2013 | J Bone Miner Res2013，，5： | 1 |
| 17 | Elect Ropolymerization of Thionine in Neutral Aqueous Medial and H2O2 Biosensor Based on Poly (Thionine) 显示文摘 | YANG Ru RUAN Chuan-min DAI Wei-lin | 1999 | Eleetrochem Acta1999，44，： | 1 |
| 18 | Influence of Iron Supplementation on DMT1(IRE)-induced Transport of Lead by Brain Barrier Systems in vivo显示文摘Objective To investigate the potential involvement of DMT1(IRE) protein in the brain vascular system in vivo during Pb exposure. Methods Three groups of male Sprague-Dawley rats were exposed to Pb in drinking water, among which two groups were concurrently administered by oral gavage once every other day as the low and high Fe treatment group, respectively, for 6 weeks. At the same time, the group only supplied with high Fe was also set as a reference. The animals were decapitated, then brain capillary-rich fraction was isolate from cerebral cortex. Western blot method was used to identify protein expression, and RT-PCR to detect the change of the m RNA. Results Pb exposure significantly increased Pb concentrations in cerebral cortex. Low Fe dose significantly reduced the cortex Pb levels, However, high Fe dose increased the cortex Pb levels. Interestingly, changes of DMT1(IRE) protein in brain capillary-rich fraction were highly related to the Pb level, but those of DMT1(IRE) m RNA were not significantly different. Moreover, the consistent changes in the levels of p-ERK1/2 or IRP1 with the changes in the levels of DMT1(IRE). Conclusion These results suggest that Pb is transported into the brain through DMT1(IRE), and the ERK MAPK pathway is involved in DMT1(IRE)-mediated transport regulation in brain vascular system in vivo. | AN Dai Zhi AI Jun Tao FANG Hong Juan SUN Ru Bao SHI Yun WANG Li Li WANG Qiang | 2015 | Biomedical and Environmental Sciences2015，28，9： | 1 |
| 19 | Eleetropolymerization of thionine in neutral aqueous media and H2O2 biosensor based on poly (thionine) 显示文摘 | YANG Ru RUAN Chuan-min DAI Wei-lin | 1999 | Electrochim Aeta1999，44，10： | 1 |
| 20 | Comparable outcomes but higher risks of prolonged viral RNA shedding duration and secondary infection in cancer survivors with COVID-19: A multi-center, matched retrospective cohort study显示文摘Results Sixty-one cancer survivors and 183 matched non-cancer patients were screened from 2,828 COVID-19 infected patients admitted to 4 hospitals in Wuhan,China.The median ages of the cancer survivor cohort and non-cancer patient cohort were 64.0(55.0–73.0)and 64.0(54.0–73.5),respectively(P=0.909).Cancer survivors reported a higher incidence of symptom onset than non-cancer patients.Fever(80.3%vs.65.0%;P=0.026)was the most prevalent symptom,followed by cough(65.6%vs.37.7%;P<0.001),myalgia,and fatigue(45.9%vs.13.6%;P<0.001).The risks of the development of severe events(adjusted hazard ratio[AHR]=1.25;95%confidence interval[CI]:0.76–2.06;P=0.378)and mortality(relative risk[RR]=0.90,95%CI:0.79–1.04;P=0.416)in the cancer survivor cohort were comparable to those of the matched non-cancer patient cohort.However,the cancer survivor cohort showed a higher incidence of secondary infection(52.5%vs.30.1%;RR=1.47,95%CI:1.11–1.95;P=0.002)and a prolonged viral RNA shedding duration(32 days[IQR 26.0–46.0]vs.24.0 days[IQR 18.0–33.0];AHR=0.54;95%CI:0.38–0.80;P<0.05).Conclusion Compared to non-cancer patients,cancer survivors with COVID-19 exhibited a higher incidence of secondary infection,a prolonged period of viral shedding,but comparable risks of the development of severe events and mortality.It is helpful for clinicians to take tailored measures to treat cancer survivors with COVID-19. | Hui Peng Sheng Wang Qi Mei Yuhong Dai Jian Li Ming Li Kathrin Halfter Xueyan Jiang Qin Huang Lei Wang Wei Wei Ru Liu Zhen cao Motuma Yigezu Daba Fangfang Wang Bingqing Zhou Hong Qiu Xianglin Yuan | 2020 | Oncology and Translational Medicine2020，6，6： | 1 |
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