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| 1 | New anti-proliferative agent,MK615,from Japanese apricot “Prunus mume” induces striking autophagy in colon cancer cells in vitro显示文摘AIM: To investigate the anti-neoplastic effects of MK615, an extract from the Japanese apricot (Prunus mume), against colon cancer cells. METHODS: Three colon cancer cell lines, SW480, COLO, and WiDr, were cultured with MK615. Growth inhibition was evaluated by cell proliferation assay and killing activity was determined by lactate dehydrogenase assay. Induction of apoptosis was evaluated by annexin Ⅴ flow cytometry. Morphological changes were studied by light and electron microscopy, and immunofluorescence staining with Atg8. RESULTS: MK615 inhibited growth and lysed SW480, COLO and WiDr cells in a dose-dependent manner. Annexin Ⅴ flow cytometry showed that MK615 induced apoptosis after 6 h incubation, at which point the occurrence of apoptotic cells was 68.0%, 65.7% and 64.7% for SW480, COLO, and WiDr cells, respectively. Light and electron microscopy, and immunofluorescence staining with Atg8 revealed that MK615 induced massive cytoplasmic vacuoles (autophagosomes) in all three cell lines. CONCLUSION: MK615 has an anti-neoplastic effect against colon cancer cells. The effect may be exerted by induction of apoptosis and autophagy. | Shozo Mori Tokihiko Sawada Toshie Okada Tatsushi Ohsawa Masakazu Adachi Kubota Keiichi | 2007 | World Journal of Gastroenterology2007，13，48： | 9 |
| 2 | Successful laparoscopic splenectomy after living-donor liver transplantation for thrombocytopenia caused by antiviral therapy显示文摘Although interferon (IFN) based therapy for recurrent hepatitis C virus (HCV) infection after liver transplantation has been widely accepted, it induces various adverse effects such as thrombocytopenia, resulting in its interruption. Recently, concomitant splenectomy at the time of living donor liver transplantation (LDLT) has been tried to overcome this problem, but this procedure leads to several complications such as excessive intraoperative bleeding and serious infection. A 60-year-old female received LDLT using a left lobe graft from her second son for liver failure caused by hepatitis C-related cirrhosis. Six months after LDLT, she was diagnosed as recurrent HCV infection by liver biopsy. IFN monotherapy was started from 7 mo after LDLT and her platelet count decreased to less than 50 000/μL, which thus made it necessary to discontinue the treatment. We decided to attempt laparoscopic splenectomy (LS) under general anesthesia. Since intra-abdominal findings did not show any adhesion formations around the spleen, LS could be successfully performed. After LS, since her platelet count immediately increased to 225 000/μL 14 d after operation, IFN therapy was restarted and we could convert the combination therapy of IFN and ribavirin, resulting in no detectable viral marker. Inconclusion, LS can be performed safely even after LDLT, and LS after LDLT is a feasible and less invasive modality for thrombocytopenia caused by antiviral therapy. | Hiroyuki Kato Masanobu Usui Yoshinori Azumi Ichiro Ohsawa Masashi Kishiwada Hiroyuki Sakurai Masami Tabata Shuji Isaji | 2008 | World Journal of Gastroenterology2008，14，26： | 7 |
| 3 | MK615 decreases RAGE expression and inhibits TAGE-induced proliferation in hepatocellular carcinoma cells显示文摘AIM:To investigate the proliferative effect of advanced glycation end-products(AGEs) and the role of their cellular receptor(RAGE) on hepatocellular carcinoma(HCC) cells,and the inhibitory effects of MK615,an extract from Japanese apricot,against AGEs were also evaluated.METHODS:Two HCC cell lines,HuH7 and HepG2,were used.Expression of RAGE was investigated by poly-merase chain reaction,Western blotting,and flow cytemetry(FACS).The effect of MK615 on RAGE expression was also evaluated by FACS.The proliferative effects of a control(unglycated bovine serum albumin),glucosederived AGEs(Glc-AGE),and glyceraldehyde-derived AGEs(Glycer-AGE),and the anti-proliferative effect of MK615 against AGEs,were evaluated using MTT assays.RESULTS:Expression of RAGE was confirmed at both the mRNA and protein levels in both HuH7 and HepG2.FACS revealed that the level of RAGE expression was higher in HuH7 than in HepG2.Treatment with 0.1 μg/mL MK615 decreased the expression level of RAGE from 24.3% to 3.7% in HuH7 and from 6.2% to 4.8% in HepG2.The growth indices for the control,Glc-AGE,and Glycer-AGE were 1.06 ± 0.08,0.99 ± 0.04,and 1.38 ± 0.05,respectively,in HuH7(P = 0.037),and were 1.03 ± 0.04,1.04 ± 0.03,and 1.07 ± 0.05,respectively,in HepG2(P > 0.05).When the cells were cultured simultaneously with Glycer-AGE and MK615,MK615 abrogated the proliferative effect of Glycer-AGE in HuH7.CONCLUSION:Only Glycer-AGE has a proliferative effect on HuH7,which expresses a higher level of RAGE.MK615 suppresses the proliferative effect of GlycerAGE on HuH7 by decreasing the expression of RAGE. | Yuhki Sakuraoka Tokihiko Sawada Toshie Okada Takayuki Shiraki Yoshikazu Miura Katsuya Hiraishi Tatsushi Ohsawa Masakazu Adachi Jun-ichi Takino Masayoshi Takeuchi Keiichi Kubota | 2010 | World Journal of Gastroenterology2010，16，42： | 6 |
| 4 | Effects of Solid Solution Treating on the Microstructureand Damping Behaviour of MnCuNiFe Alloys显示文摘一个高抑制条件容易在 Mn-(16-24 )Cu-(4-6 ) Ni-2Fe 被获得(在。%) 合金,当冷却时,率在 1173 K 在稳固的答案处理以后被控制。在 10 h 的对数的减少的温度依赖者变化使样品凉下来,这被观察对合金作文敏感。作为与熄灭处理的水相比,控制了 10 h 冷却由在合金生产充实 Mn 的矩阵部分广泛地改进合金的 T//N 温度。计算建议在矩阵部分的 Cu 和 Ni 内容的相对减少被 Ni 内容在原来的合金统治,并且因此, Cu 的体积部分,充实 Ni 猛抛被估计分别地在 4Ni 和 6Ni 合金是大约 20% 和 10% 。作为结果,为每合金的 T//N 温度,相应于对数的减少的升起的温度,在充实 Mn 的矩阵与 Cu,和 Ni 有关。左钩住的存在 110 条正确钩住 twinning 边界在 4Ni 和 6Ni 合金的微观结构被证实。然而,相对格子紧张, twinning 边界扮演提供,在二合金被发现大部分不同。有更小的取向偏差的边界能由周期的移动容易协调外部压力,这被考虑,因此,发生在 10 h 的更宽广的抑制山峰使 6Ni 凉下来合金变得可行。电子衍射结果也在矩阵阶段显示许多亚水晶的可能的形成,它相对在一些不变的飞机上被旋转。那些亚水晶边界可能在在 T//N 和房间温度之间的温度范围起一些变细作用。(编辑作者摘要) 9 个裁判员。 | Yin, Fuxing Ohsawa, Y. Sato, A. Kawahara, K. | 1998 | Journal of Materials Science & Technology1998，14，4： | 3 |
| 5 | ESTIMATION OF CHAIN REACTION BANKRUPTCY STRUCTURE BY CHANCE DISCOVERY METHO—WITH TIME ORDER METHOD AND DIRECTED KEYGRAPH显示文摘Chain reaction bankruptcy is regarded as common phenomenon and its effect is to be taken into account when credit risk portfolio is analyzed.But consideration and modeling of its effect leave much room for improvement.That is mainly because method for grasping relations among companies with limited data is underdeveloped.In this article,chance discovery method is applied to estimate industrial relations that are to include companies' relations that transmit chain reaction of bankruptcy. Time order method and directed KeyGraph are newly introduced to distinguish and express the time order among defaults that is essential information for the analysis of chain reaction bankruptcy.The steps for the data analysis are introduced and result of example analysis with default data in Kyushu, Japan,2005 is presented.The structure estimated by the new method is compared with the structure of actual account receivable holders of bankrupted companies for evaluation. | Shinichi GODA Yukio OHSAWA | 2007 | Journal of Systems Science and Systems Engineering2007，16，4： | 3 |
| 6 | DATA CRYSTALLIZATION APPLIED FOR DESIGNING NEW PRODUCTS显示文摘它仅仅是能在数据被存储的真实世界的可见部分。为如此的不完全、组织病的数据,结晶的数据瞄准 at presenting 在包括 unobservable 事件的事件之中的隐藏的结构。,这被数据结晶化认识到哑巴项目,相应于 unobservable 事件的潜在的存在,被插入到给定的数据。有可见事件的这些哑巴项目和他们的关系被 applyingKeyGraph 与哑巴项目设想到数据,象灰尘涉及水分子的结晶化的形成的雪的结晶化一样。为调节要设想的结构的颗粒度水平,数据结晶化的工具与人在真实世界上理解重要情形的过程是综合的。这个基本方法被期望为机会发现的以前的方法带人到成功的决策的各种各样的真实世界领域适用。在这篇论文,我们在一个真实公司与 human-interactiveannealing (DCHA ) 把数据结晶化用于产品的设计。结果显示出它的效果到工业决策。 | Kenichi HORIE Yoshiharu MAENO Yukio OHSAWA | 2007 | Journal of Systems Science and Systems Engineering2007，16，1： | 3 |
| 7 | Evaluation Method for Offshore Wind Energy Resources Using Scatterometer and Weibull Parameters显示文摘 | Katsutoshi Kozai Teruo Ohsawa Rinya Takahashi Yuko Takeyama | 2012 | Journal of Energy and Power Engineering2012，6，11： | 2 |
| 8 | Hydration of fly ash cement显示文摘 | Etsuo Sakai Shigeyoshi Miyahara Shigenari Ohsawa Seung-Heun Lee Masaki Daimon | 2004 | Cement and Concrete Research2004，，6： | 2 |
| 9 | Modeling Alzheimer’s Disease with iPSCs Reveals Stress Phenotypes Associated with Intracellular Aβ and Differential Drug Responsiveness显示文摘 | Takayuki Kondo Masashi Asai Kayoko Tsukita Yumiko Kutoku Yutaka Ohsawa Yoshihide Sunada Keiko Imamura Naohiro Egawa Naoki Yahata Keisuke Okita Kazutoshi Takahashi Isao Asaka Takashi Aoi Akira Watanabe Kaori Watanabe Chie Kadoya Rie Nakano Dai Watanabe Kei | 2013 | Cell Stem Cell2013，，4： | 2 |
| 10 | Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial ischemia–reperfusion injury显示文摘 | Kentaro Hayashida Motoaki Sano Ikuroh Ohsawa Ken Shinmura Kayoko Tamaki Kensuke Kimura Jin Endo Takaharu Katayama Akio Kawamura Shun Kohsaka Shinji Makino Shigeo Ohta Satoshi Ogawa Keiichi Fukuda | 2008 | Biochemical and Biophysical Research Communications2008，，1： | 2 |
| 11 | Generation and characterization of a microglial cell line,MG5, derived from a p53-deficient mouse显示文摘 | OHSAWA K IMAI Y NAKAJIMA K | 1997 | Glia1997，21，： | 1 |
| 12 | Hydrogen therapy attenuates irradiation - induced lung damage by reducing oxidative stress 显示文摘 | Terasaki Y Ohsawa I Terasaki M | 2011 | Am J Physiol Lung Cell Mol Physiol2011，301，4： | 1 |
| 13 | Involvement of Ibal in membrane ruffling and phagocytosis of macrophages/microglia显示文摘 | Ohsawa K lmai Y Kanazawa H | 2000 | Journal of Cell Science2000，113，17： | 1 |
| 14 | Necrotic death pathway in Fas receptor signaling显示文摘 | Matsumura H Shimizu Y Ohsawa Y | | 0，，13： | 1 |
| 15 | Kappa-opioid recep- tor-mediated antinociceptive effects of stereoisomer, and derivatives of (+) - matrine in mice显示文摘 | Xiao P Kubo H Ohsawa M | 1999 | Planta Med1999，65，3： | 1 |
| 16 | Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial ische- mia-reperfusion injury 显示文摘 | Hayashida K Sano M Ohsawa I | 2008 | Biochem Biophys Res Commun2008，373，1： | 1 |
| 17 | Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice显示文摘 | Ohsawa I Nishimaki K Yamagata K | 2008 | BBRC2008，377，4： | 1 |
| 18 | Use of immunohistochemical procedures in diagnosing angiosarcoma : evaluation of 98 cases 显示文摘 | Ohsawa M Naka N Tonmita Y | 1995 | Cancer1995，75，12： | 1 |
| 19 | Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals显示文摘 | Ohsawa I Ishikawa M Takahashi K | 2007 | Nat Med2007，13，6： | 1 |
| 20 | Localization ofoxidized phosphatidylcholine in nonalcoholic fatty liverdisease ; impact on disease progression 显示文摘 | Ikura Y Ohsawa M Suekane T | 2006 | Hepatology2006，43，3： | 1 |
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