|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 尿钠排泄、血压、心血管病与死亡率的关系:社区前瞻性流行病学队列研究显示文摘WHO建议人群钠摄人<2 g/d作为预防心血管病的措施之一,但这一目标在任何国家都没有实现。该建议主要基于短期血压试验的个体水平数据,没有来自随机试验或观察性研究的关于低钠摄入与降低心血管事件的数据。前瞻性城市农村流行病学研究目前正在21个国家进行。该文对其中18个国家的数据进行分析,其中包含了临床结果数据。 | Mente A O'Donnell M Rangarajan S McQueen M Dagenais G Wielgosz A Lear S Ah STL Wei L Diaz R Avezum A Lopez-Jaramillo P Lanas F Mony P Szuba A Iqbal R Yusuf R Mohammadifard N Khatib R Yusoff K Ismail N Gulec S Rosengren A Yusufali A Kruger L Tsolekile LP Chifamba J Dans A Alhabib KF Yeates K Teo K Yusuf S 刘莉 叶鹏 | 2018 | 中华高血压杂志2018，26，12： | 29 |
| 2 | Sorafenib inhibits growth and metastasis of hepatocellular carcinoma by blocking STAT3显示文摘AIM: To investigate the inhibitory role and the underlying mechanisms of sorafenib on signal transducer and activator of transcription 3 (STAT3) activity in hepatocellular carcinoma (HCC).METHODS: Human and rat HCC cell lines were treated with sorafenib. Proliferation and STAT3 dephosphorylation were assessed. Potential molecular mechanisms of STAT3 pathway inhibition by sorafenib were evaluated. In vivo antitumor action and STAT3 inhibition were investigated in an immunocompetent orthotopic rat HCC model.RESULTS: Sorafenib decreased STAT3 phosphorylationat the tyrosine and serine residues (Y705 and S727), but did not affect Janus kinase 2 (JAK2) and phosphatase shatterproof 2 (SHP2), which is associated with growth inhibition in HCC cells. Dephosphorylation of S727 was associated with attenuated extracellular signal-regulated kinase (ERK) phosphorylation, similar to the effects of a mitogen-activated protein kinase (MEK) inhibitor U0126, suggesting that sorafenib induced S727 dephosphorylation by inhibiting MEK/ERK signaling. Meanwhile, sorafenib could also inhibit Akt phosphorylation, and both the phosphatidylinositol-3-kinase (PI3K) inhibitor LY294002 and Akt knockdown resulted in Y705 dephosphorylation, indicating that Y705 dephosphorylation by sorafenib was mediated by inhibiting the PI3K/Akt pathway. Finally, in the rat HCC model, sorafenib signifi cantly inhibited STAT3 activity, reducing tumor growth and metastasis.CONCLUSION: Sorafenib inhibits growth and metastasis of HCC in part by blocking the MEK/ERK/STAT3 and PI3K/Akt/STAT3 signaling pathways, but independent of JAK2 and SHP2 activation. | Fang-Ming Gu, Quan-Lin Li, Qiang Gao, Jia-Hao Jiang, Xiao-Yong Huang, Jin-Feng Pan, Jia Fan, Jian ZhouFang-Ming Gu, Quan-Lin Li, Qiang Gao, Jia-Hao Jiang, Xiao-Yong Huang, Jin-Feng Pan, Jia Fan, Jian Zhou, Liver Cancer Institute, Zhongshan Hospital and Shanghai Medical School, Fudan University, Shanghai 200032, China Author contributions: Gu FM, Li QL and Gao Q contributed equally to this work Gu FM and Li QL performed the experi- ments and interpretation of the data and statistical analysis Zhou J and Gao Q contributed to the conception and design of the study Gu FM, Gao Q, Li QL and Zhou J wrote the manuscript Jiang JH, Huang XY, Pan JF and Fan J made substantial contri- bution to the design and conception of the study and interpreta- tion of data all authors read and approved the f inal manuscript. | 2011 | World Journal of Gastroenterology2011，17，34： | 18 |
| 3 | 采用特殊加工助剂改善绿色轮胎胎面胶性能显示文摘混炼白炭黑补强的S-SBR/BR轿车轮胎胎面胶料时,采用特殊的混炼程序可以获得最佳硅烷偶联的效果.在此混炼程序中,一段混炼仅加入生胶、填料、硅烷和油,二段混炼添加氧化锌、硬脂酸、防老剂和蜡.采用这种程序虽然可以获得最佳硅烷偶联效果,但可能会引起填料分散差和硅烷降解,而且还有添加的低熔点材料的润滑效应引起返炼初始胶料包辊性差的问题.分别采用常规混炼程序和特殊混炼程序制备了胶料,在一段混炼中分别添加和不添加特殊加工助剂.采用特殊程序混炼的胶料,在二段混炼中分别添加和不添加表面活性剂类加工助剂.一段混炼添加的加工助剂是专为不影响硅烷偶联效果而开发的.由于添加剂的润滑作用使返炼时混炼效果差,可以通过用表面活性剂类加工助剂代替硬脂酸加以改善.对未硫化胶的粘弹动态试验可以使混炼质量各个方面的单独量化成为可能.试验数据清楚地表明,在一段和二段混炼中添加的加工助剂对于白炭黑分散和硅烷偶联有积极的作用,而且它们的作用是叠加的.物理性能数据还显示出这些加工助剂的优点.对硫化样品进行的粘弹动态试验可用于预测轮胎使用性能,而且也再次证明了使用这些加工助剂的优越性. | Stone C R Menting K H Hensel M 涂学忠 | 2001 | 轮胎工业2001，21，4： | 18 |
| 4 | The IKK-related kinases: from innate immunity to oncogenesis显示文摘在过去的四年,天生的有免疫力的反应的地被 kinase ( IKK )联系了 kinases ,坦克有约束力的 Kinase 1 ( TBK1 )和 IKKi 的 IB 的发现高度影响了,它调整干扰素的活动规章的因素( IRF ) -3/IRF-7 和 NF-B 抄写因素。更最近,激活这些 IKK 相当或相同事物的发信号的小径的另外的必要部件被发现了。这些包括 RNA helicases RIGi 和 MDA5,和作为 CARDIF/MAVS/VISA/IPS-1 知道的下游的 mitochondrial 受动器。除了他们在控制天生的有免疫力的反应的必要功能,最近的研究在房间增长和 oncogenesis 加亮这些 kinases 的一个角色。正规 IKK 很好被认出是连接长期的发炎到癌症的一座桥。新调查结果现在建议 IKK 相关的 kinases TBK1 和 IKKi 也参予影响房间转变和肿瘤前进的发信号的小径。这评论将因此由集中于他们的规定和底层特性在细胞的转变和 oncogenesis 总结并且讨论 TBK1 和 IKKi 的角色。 | Jean-Francois Clément | 2008 | Cell Research2008，18，9： | 16 |
| 5 | 内脏脂肪组织通过产生骨桥蛋白调控成纤维细胞衰老促进心脏衰老显示文摘老化通过增加包括骨桥蛋白(osteopontin,OPN)在内的细胞外基质(extracellular matrix,ECM)蛋白的沉积引起心脏结构和功能改变进而导致进行性间质纤维化。虽然已知OPN参与各种病理状况,但其在心肌衰老中的作用仍不清楚。 | Sawaki D Czibik G Pini M Ternacle J Suffee N Mercedes R Marcelin G Surenaud M Marcos E Gual P Clément K Hue S Adnot S Hatem SN Tsuchimochi I Yoshimitsu T Hénégar C Derumeaux G 刘青 叶鹏 | 2018 | 中华高血压杂志2018，26，3： | 14 |
| 6 | Clinical relationship between MDR1 gene and gallbladder cancer显示文摘BACKGROUND: The most common mechanisms of mul- tidrug resistance (MDR) in cancer cells is the expression of an energy-dependent exfflux pump. P-glycoprotein (P-gp) encoded by MDR1 gene and multidrug associated protein (MRP) are well known proteins associated with MDR. In human cancers, the MDR1 gene expression is common in patients with intrinsic and acquired MDR. It is a major therapeutic problem in cancer chemotherapy. Previously we found that the MDR of HCC is related to MRP gene ex- pression and initiates the intrinsic MDR. The aim of this study is to study the expression of MDR1 gene encoding P-gp and MDR1 mRNA in primary gallbladder carcinoma, and analyze its clinical significance. METHODS: Immunohistochemistry (IHC) S-P method and in situ polymerase chain reaction (ISPCR) were used to detect the expression of P-gp and MDR1 mRNA in 53 cases of untreated primary gallbladder carcinoma and 12 ca- ses of cholecystitis (archival paraffin-embedded tissues). RESULTS: The positive expression rates of P-gp and MDR1 mRNA in the 53 cases and 12 cases were 60.38%, 71.69% and 25.00%, 33.33%, respectively. There was a significant difference between the two groups (P<0.05). The positive expression rate of P-gp and MDRlmRNA were 69.44%, 83.33% and 41.18%, 47.06% respectively in tissues in stage of Nevin against Nevin , (P<0.05). In well, moderately differentiated gallbladder carcinoma tissues, their expressions were 79.49%, 69.23% against 50.00%, 35.71% in low, undifferentiated tissues (P<0.05). CONCLUSIONS: MDR to gallbladder carcinoma is closely related to the intrinsic MDR and it provides an important evidence to reverse the MDR by detection of the MDR1gene. Meanwhile, MDR1 gene expression in gallbladder carcinoma is correlated with some biological characteris- tics , takes part in the carcinogenesis of gallbladder tissues, and acts as a valuable biomarker of prognosis. | Bai-Lin Wang, Hai-Ying Zhai, Bing-Yi Chen, Shu-Ping Zhai, Hai-Yan Yang, Xiao-Ping Chen, Wen-Tao Zhao and Lei Meng Guangzhou, China Third Department of Surgery, First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China Depart- ment of Surgery, First People’s Hospital, Jining 272100, China Department of Surgery, General Hospital of PLA, Beijing 100853, China and Hepatic Surgery Center, Tongji Hospital Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，2： | 9 |
| 7 | 新型远红外Ge-Te-Se-Sn硫系玻璃的热学与光学性质研究显示文摘采用熔融淬冷法制备了新型远红外Te基硫系玻璃Ge20-xTe65Se15Snx(x=0,2,4)。在Ge-Te-Se玻璃的基础上,通过引入重金属Sn,研究其对玻璃组成、结构和性能的影响。测试了样品的中红外热成像、X射线衍射(XRD)图谱、差热分析曲线(DSC)、可见/近红外吸收光谱、红外透射光谱。利用经典的Tauc方程计算了样品的光学带隙,根据金属标准和能量带隙理论讨论了玻璃光学带隙与组分变化的关系。分析结果表明,Ge-Te-Se-Sn玻璃具有良好的热稳定性,随着Sn的引入,玻璃的光学带隙减小,短波吸收截止边发生红移,但玻璃的红外截止波长基本不变。此外Sn可以有效减弱Ge-O杂质吸收峰的强度,提高玻璃红外透射性能。 | 孙杰 聂秋华 王训四 戴世勋 徐铁峰 章向华 Bruno Bureau Catherine Boussard Clément Conseil | 2011 | 光学学报2011，31，11： | 6 |
| 8 | Electrochemical ageing study of mixed lanthanum/praseodymium nickelates La2-xPrxNiO4+δ as oxygen electrodes for solid oxide fuel or electrolysis cells显示文摘The chemical and electrochemical stability of lanthanide nickelates La2 NiO4+δ(LNO),Pr2 NiO4+δ(PNO)and their mixed compounds La(2-x)PrxNiO4+δ(LPNOs)with x=0.5,1 or 1.5 is reported.The aim is to promote these materials as efficient electrodes for solid oxide fuel cell(SOFC)and/or solid oxide electrolysis cell(SOEC).La2 NiO4+δand La1.5Pr0.5NiO4+δcompounds are chemically very stable as powders over one month in the temperature range 600-800℃,while the other materials rich in praseodymium progressively decompose into various perovskite-deriving components with additional Pr6 O11.Despite their uneven properties,all these materials are quite efficient and sustainable as electrodes on top of gadolinium doped ceria(GDCBL)//yttrium doped zirconia(8 YSZ)electrolyte,for one month at 700℃without polarization.Under polarization(300 mA·cm-2),the electrochemical performances of LNO,PNO and La1.5Pr0.5NiO4+δ(LP5 NO)quickly degrade in SOFC mode,i.e.for the oxygen reduction reaction,while they show durability in SOEC mode,i.e.for the oxide oxidation reaction. | Vaibhav Vibhu Aurélien Flura Aline Rougier Clément Nicollet Sébastien Fourcade Teresa Hungria Jean-Claude Grenier Jean-Marc Bassat | 2020 | Journal of Energy Chemistry2020，29，7： | 6 |
| 9 | 肛门内括约肌侧切术对慢性肛裂患者生活质量的影响 | Mente B.B. Tezcaner T. Yilmaz U. 孟欣颖 | 2006 | 世界核心医学期刊文摘(胃肠病学分册)2006，0，12： | 5 |
| 10 | Magnesium sulfate and fetal neuroprotection: overview of clinical evidence显示文摘Antenatal administration of magnesium sulfate is an important part of the neuroprotective strategy for preterm infants. Strong evidence from five randomized controlled trials and five meta-analyses has demonstrated that magnesium sulfate, when administered before preterm delivery, significantly reduces the risk of cerebral palsy at two years. Through secondary analyses of randomized controlled trials and other original clinical studies, this state-of-the-art review highlights the absence of serious adverse effects in both pregnant women and neonates, as well as the impact of maternal body mass index and preeclamptic status on the maternal and neonatal magnesium levels, which could influence the magnitude of the neuroprotective effect. Although antenatal magnesium sulfate is a cost-effective strategy, some practice surveys have demonstrated that the use of magnesium sulfate is not sufficient and that its use is heterogeneous, differing among different maternity wards. Since 2010, an increasing number of obstetrical societies have recommended its use to improve the neurological outcomes of preterm infants, especially the International Federation of Gynecology and Obstetrics and World Health Organization in 2015, and France in 2017. Considering the neuroprotective impact of magnesium sulfate when administered before delivery, postnatal administration should be considered, and its effects should be assessed using randomized controlled trials. | Clément Chollat Stéphane Marret | 2018 | Neural Regeneration Research2018，13，12： | 5 |
| 11 | Effects of Dan-Shao-Hua-Xian on the expression of collagen type I and III in rats with hepatic fibrosis显示文摘BACKGROUND: Hepatic fibrosis is the common patholo gical change in various chronic liver diseases,and its major cause is the imbalance between the production and degra dation of the extracellular matrix, which is mainly com posed of collagens. Dan-Shao-Hua-Xian ( DSHX) cap sule, a traditional Chinese herbal compound, has shown marked preventive effects on hepatic fibrosis in rats in our previous studies. The present study was designed to further investigate its therapeutic actions on hepatic fibrosis in rats and its possible mechanisms. METHODS: Eighty male Wistar rats were randomly divid- ed into normal control group, hepatic fibrosis group, non DSHX-treated group, low-dose-treated group, and high dose-treated group. The rat models of hepatic fibrosis were established by subcutaneous injecton of CC14 , drinking al- cohol , giving diet of hyperliprosis and hypoprotein for 8 weeks. The two DSHX-treated groups were treated respec- tively with low dose (0.5 g/kg) and high dose (1.0 g/kg) of DSHX capsule p. o. everyday for 8 weeks. At the end of the experiment, liver indexes were calculated in each group in addition to the levels of the serum hyaluronic acid and alanine aminotransferase. Their degree of hepatic fibrosis and urinary excretion of hydroxyproline and expression of collagen , were detected. RESULTS: Comparison of the indexes of the hepatic fibro- sis group and non-DSHX-treated group revealed that the liver indexes, levels of serum hyaluronic acid and alanine aminotransferase, and stage of hepatic fibrosis were all sig- nificantly reduced in the two DSHX treated groups. The urinary excretion of hydroxyproline was increased and the expression of collagen I and HI in liver tissue was lessened. These alterations were more obviously observed in the high-dose-treated group. CONCLUSION: DSHX capsule has certain therapeutic effect on hepatic fibrosis in rats. | Xiao-Xia Geng, Qin Yang, Ru-Jia Xie, Xin-Hua Luo, Cheng-Xiu Li and Ming-Liang Cheng Guizhou, China Department of Infectious Diseases, Affiliated Hospital of Guiyang Medical College Department of Pathophysiology, Guiyang Medical College Depart- ment of Infectious Diseases, People’ s Hospital of Guizhou Province and Department of Pharmacology, Guiyang Medical College | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，4： | 4 |
| 12 | Prokineticin 2/Bv8 is expressed in Kupffer cells in liver and is down regulated in human hepatocellular carcinoma显示文摘AIM: To study the implication of prokineticin 1 (PK1/EG- VEGF) and prokineticin 2 (PK2/Bv8) in hepatocellular carcinoma angiogenesis. METHODS: The gene induction of PK1/EG-VEGF and PK2/Bv8 was investigated in 10 normal, 28 fibrotic and 28 tumoral livers by using real time PCR. Their expression was compared to the expression of VEGF (an angiogenesis marker), vWF (an endothelial cell marker) and to CD68 (a monocyte/macrophage marker). Furthermore, the mRNA levels of PK1/EG-VEGF, PK2/Bv8, prokineticin receptor 1 and 2 were evaluated by real time PCR in isolated liver cell populations. Finally, PK2/Bv8 protein was detected in normal liver paraffin sections and in isolated liver cells by immunohistochemistry and immunocytochemistry. RESULTS: PK2/Bv8 mRNA but not PK1/EG-VEGF was expressed in all types of normal liver samples examined. In the context of liver tumor development, we reported that PK2/Bv8 correlates only with CD68 and showed a significant decrease in expression as the pathology evolves towards cancer. Whereas, VEGF and vWF mRNA were significantly upregulated in both fibrosis and HCC,as expected. In addition, out of all isolated liver cells examined, only Kupffer cells (liver resident macrophages) express significant levels of PK2/Bv8 and its receptors, prokineticin receptor 1 and 2. CONCLUSION: In normal liver PK2/Bv8 and its receptors were specifically expressed by Kupffer cells. PK2/Bv8 expression decreased as the liver evolves towards cancer and did not correlate with HCC angiogenesis. | Justin Monnier Claire Piquet-Pellorce Jean-Jacques Feige Orlando Musso Bruno Clément Bruno Turlin Nathalie Théret Michel Samson | 2008 | World Journal of Gastroenterology2008，14，8： | 4 |
| 13 | 尿钠和钾排泄与血压的关系显示文摘高血压影响着全球10亿人口,是死亡、脑卒中、心肌梗死、充血性心力衰竭和慢性肾损伤的主要原因。钠摄入是高血压一个可调控的决定因素。盐和血压国际性研究(international study of salt and blood pressure,INTERSALT)结果显示钠摄入和血压呈中等程度正相关[1]。然而INTERSALT不是一个足够大型的研究,其钠摄入和血压的相关性是否受地区、参与人群特征和钠或钾摄入水平影响还有待于进一步研究。 | Mente A O'Donnell MJ Rangarajan S 练桂丽 叶鹏 | 2014 | 中华高血压杂志2014，22，9： | 4 |
| 14 | Development and evaluation of a safe and effective sugar-free herbal lollipop that kills cavity-causing bacteria显示文摘Dental caries (tooth decay) is caused by a specific group of cariogenic bacteria, like Streptococcus mutans, which convert dietary sugars into acids that dissolve the mineral in tooth structure. Killing cariogenic bacteria is an effective way to control or prevent tooth decay. In a previous study, we discovered a novel compound (Glycyrrhizol A), from the extraction of licorice roots, with strong antimicrobial activity against cariogenic bacteria. In the current study, we developed a method to produce these specific herbal extracts in large quantities, and then used these extracts to develop a sugar-free lollipop that effectively kills cariogenic bacteria like Streptococcus mutans. Further studies showed that these sugar-free lollipops are safe and their antimicrobial activity is stable. Two pilot human studies indicate that a brief application of these lollipops (twice a day for ten days) led to a marked reduction of cariogenic bacteria in oral cavity among most human subjects tested. This herbal lollipop could be a novel tool to promote oral health through functional foods. | Chu-hong Hu Jian He Randal Eckert Xiao-yang Wu Li-na Li Yan Tian Renate Lux Justin A. Shuffer Faina Gelman Janet Mentes Sue Spackman Janet Bauer Maxwell H. Anderson Wen-yuan Shi | 2011 | International Journal of Oral Science2011，3，1： | 3 |
| 15 | Volumetric Changes after 90 Y Radioembolization for Hepatocellular Carcinoma in Cirrhosis: An Option to Portal Vein Embolization in a Preoperative Setting?显示文摘 | Julien Edeline Laurence Lenoir Karim Boudjema Yan Rolland Anne Boulic Fanny Le Du Marc Pracht Jean-Luc Raoul Bruno Clément Etienne Garin Eveline Boucher | 2013 | Annals of Surgical Oncology2013，，8： | 3 |
| 16 | Phase Diversity Electro-optic Sampling:A new approach to single-shot terahertz waveform recording显示文摘Recording electric field evolution in single-shot with THz bandwidth is needed in science including spectroscopy,plasmas,biology,chemistry,Free-Electron Lasers,accelerators,and material inspection.However,the potential application range depends on the possibility to achieve sub-picosecond resolution over a long time window,which is a largely open problem for single-shot techniques.To solve this problem,we present a new conceptual approach for the so-called spectral decoding technique,where a chirped laser pulse interacts with a THz signal in a Pockels crystal,and is analyzed using a grating optical spectrum analyzer.By borrowing mathematical concepts from photonic time stretch theory and radio-frequency communication,we deduce a novel dual-output electro-optic sampling system,for which the input THz signal can be numerically retrieved—with unprecedented resolution—using the so-called phase diversity technique.We show numerically and experimentally that this approach enables the recording of THz waveforms in single-shot over much longer durations and/or higher bandwidth than previous spectral decoding techniques.We present and test the proposed DEOS(Diversity Electro-Optic Sampling)design for recording 1.5 THz bandwidth THz pulses,over 20 ps duration,in single-shot.Then we demonstrate the potential of DEOS in accelerator physics by recording,in two successive shots,the shape of 200 fs RMS relativistic electron bunches at European X-FEL,over 10 ps recording windows.The designs presented here can be used directly for accelerator diagnostics,characterization of THz sources,and single-shot Time-Domain Spectroscopy. | Eléonore Roussel Christophe Szwaj Clément Evain Bernd Steffen Christopher Gerth Bahram Jalali Serge Bielawski | 2022 | Light(Science & Applications)2022，11，1： | 3 |
| 17 | 树突状细胞自然杀伤细胞凝集素群受体1促进小鼠动脉粥样硬化的发展显示文摘动脉粥样硬化发展过程中的坏死核心形成与慢性炎症反应有关,其促进斑块发展加快和不稳定。然而,坏死与动脉粥样硬化发展之间的分子生物学联系机制尚未完全清楚。 | 刘莉 叶鹏 Haddad Y Lahoute C Clément M Laurans L Metghalchi S Lynda Z Giraud A Loyer X Vandestienne M Wain-Hobson J Esposito B Potteaux S Ait-Oufella H Tedgui A Mallat Z Taleb S | 2017 | 中华高血压杂志2017，25，8： | 3 |
| 18 | phosphatase and tensin homolog is a differential diagnostic marker between nonalcoholic and alcoholic fatty liver disease显示文摘AIM: To investigate the protein expression of phosphatase and tensin homolog(PTEN) in human liver biopsies of patients with alcoholic and non-alcoholic liver disease.METHODS: PTEN protein expression was assessed by immunohistochemistry in formalin-fixed, paraffinembedded liver sections of patients with non-alcoholic fatty liver disease(NAFLD)(n = 44) or alcoholic liver disease(ALD)(n = 25). Liver resections obtained from 3 healthy subjects candidate for partial liver donation served as controls. Histological evaluations were performed by two experienced pathologists, and diagnoses established based on international criteria. The intensity of the PTEN staining in nuclei was compared between steatotic and non-steatotic areas of each liver fragment analyzed. For each liver specimen, the antibody-stained sections were examined and scored blindly by three independent observers, who were unaware of the patients' clinical history.RESULTS: In healthy individuals, PTEN immunostaining was intense in both the cytoplasm and nuclei of all hepatocytes. However, PTEN was strongly downregulated in both the nucleus and the cytoplasm of hepatocytes from steatotic areas in patients with NAFLD, independently of the disease stage. In contrast, no changes in PTEN protein expression were observed in patients with ALD, regardless of the presence of steatosis or the stage of the disease. The degree of PTEN downregulation in hepatocytes of patients with NAFLD correlated with the percentage of steatosis(r = 0.3061, P = 0.0459) and the BMI(r = 0.4268, P = 0.0043). Hovewer, in patients with ALD, PTEN expression was not correlated with the percentage of steatosis with or without obesity as a confounding factor(P = 0.5574). Finally, PTEN expression level in steatotic areas of ALD patients was significantly different from that seen in steatotic areas of NAFLD patients(P < 0.0001).CONCLUSION: PTEN protein expression is downregulated early in NAFLD, but not in ALD. PTEN immunohistochemical detection could help in the differential diagnosis of NAFLD and ALD. | Andrea Sanchez-Pareja Sophie Clément Marion Peyrou Laurent Spahr Francesco Negro Laura Rubbia-Brandt Michelangelo Foti | 2016 | World Journal of Gastroenterology2016，22，14： | 2 |
| 19 | Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage显示文摘Background: Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results. The purpose of this study was to identify the most stable housekeeping genes in porcine articular cartilage subjected to a mechanical injury from a panel of 10 candidate genes. Results: Ten candidate housekeeping genes were evaluated in three different treatment groups of mechanically impacted porcine articular cartilage. The genes evaluated were: beta actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hydroxymethylbilane synthase, hypoxanthine phosphoribosyl transferase, peptidylprolyl isomerase A (cyclophilin A), ribosomal protein L4, succinate dehydrogenase flavoprotein subunit A, TATA box binding protein, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein-zeta polypeptide. The stability of the genes was measured using geNorm, BestKeeper, and NormFinder software. The four most stable genes measured via geNorm were (most to least stable) succinate dehydrogenase flavoprotein, subunit A, peptidylprolyl isomerase A, glyceraldehyde-3-phosphate dehydrogenase, beta actin; the four most stable genes measured via BestKeeper were glyceraldehyde-3-phosphate dehydrogenase, peptidylprolyl isomerase A, beta actin, succinate dehydrogenase flavoprotein, subunit A; and the four most stable genes measured via NormFinder were peptidylprolyl isomerase A, succinate dehydrogenase flavoprotein, subunit A, glyceraldehyde-3-phosphate dehydrogenase, beta actin. Conclusions: BestKeeper, geNorm, and NormFinder all generated similar results for the most stable genes in porcine articular cartilage. The use of these appropriate reference genes will facilitate accurate gene expression studies of porcine articular cartilage and suggest appropriate housekeeping genes for articular cartilage studies in other species. | Ryan S McCulloch Melissa S Ashwell Audrey T O'Nan Peter L Mente | 2012 | Journal of Animal Science and Biotechnology2012，3，4： | 2 |
| 20 | Early detection of apple bruises on different background colors using hyperspectral imaging显示文摘 | Gamal ElMasry Ning Wang Clément Vigneault Jun Qiao Adel ElSayed | 2007 | LWT - Food Science and Technology2007，，2： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员