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| 1 | 亚麻籽环肽混合物抑制霉菌生长繁殖活性显示文摘选取宛氏拟青霉(AS 3.776)和黄曲霉(CICC 2476)作为试验菌株,于121℃高温灭菌前、后添加一定浓度梯度的亚麻籽环肽混合物(flaxseed cyclolinopeptides,FCLPs)到马铃薯葡萄糖琼脂培养基中。FCLPs对宛氏拟青霉孢子形成的半数抑制浓度IC50分别为0.53 mg/m L(高温灭菌)和0.34 mg/m L(未经高温灭菌);对黄曲霉孢子形成的IC50值分别为0.31 mg/m L(高温灭菌)和0.24 mg/m L(未经高温灭菌)。高温灭菌处理后,FCLPs保持了相当的抑菌活性,对宛氏拟青霉的抑制活性最低残留76.8%(菌落生长)和76.7%(孢子形成);对于黄曲霉的抑制活性,最低残留分别为61.6%(菌落生长)和41.7%(孢子形成)。FCLPs对孢子萌发率的平均抑制率为17.8%(宛氏拟青霉)和7.8%(黄曲霉)。采用高效液相色谱法分析霉菌培养后从培养基中提取的FCLPs,发现高温灭菌会降低环肽的含量,这可能是导致环肽抗菌活性下降的原因之一,但环肽中4种主要环肽的比例没有发生明显变化,且没有发现环肽霉菌代谢物产生。综上所述,FCLPs对霉菌具有一定抑制活性,对高温处理较为稳定,能耐食品的热加工,相比现有抗菌肽具有独特优势。 | 刘尊 汪勇 MARTIN J T REANEY FIORENTY OCTAVIA SUGIWAN NANDA AYU YULISTYANITA 张宁 | 2018 | 食品与发酵工业2018，44，3： | 11 |
| 2 | HPLC外标法测定亚麻籽油中4种环肽含量的研究显示文摘以环肽标准样品定性,采用外标法定量,建立了HPLC测定亚麻籽油中环肽A、环肽C、环肽D和环肽E的方法。采用固相萃取法提取亚麻籽油中的环肽,用Diamonsil C18色谱柱,以乙腈和水为流动相梯度洗脱,柱温35℃,二极管阵列检测器在波长214 nm处进行检测,30 min内完成对4种环肽的分离检测。结果表明:该方法的线性范围为10-160μg/m L,相关系数大于等于0.999 5;环肽A、环肽C、环肽D、环肽E的平均加标回收率分别为94.62%、92.25%、84.07%和100.14%。用该方法测定不同工艺制取的亚麻籽油中环肽的含量,发现未经过精炼的亚麻籽油中环肽含量高于市售亚麻籽油的,其中,冷榨工艺比热榨工艺更能保留亚麻籽油中的环肽。 | 连莹君 江紫琦 陆学茸 滕英来 REANEY Martin J T 汪勇 | 2015 | 中国油脂2015，40，5： | 9 |
| 3 | 棕榈油甲酯制备生物润滑油三羟甲基丙烷脂肪酸三酯的工艺研究显示文摘以棕榈油脂肪酸甲酯(fatty acid methyl ester,FAME)和三羟甲基丙烷(trimethylolpropane,TMP)为原料,经酯交换反应制备可用作生物润滑油基础油的三羟甲基丙烷脂肪酸三酯(trimethylolpropane fatty acidtiester,TFATE)。通过比较FAME的转化率和TFATE在反应混合物中的含量,探讨了催化剂种类、催化剂用量、反应时间、反应温度、反应物物质的量比及真空度对反应的影响。最佳合成条件为:催化剂为三羟甲基丙烷钾(Trimethylolpropane mono potassium salt,TMP-K),催化剂添加量为TMP-K与TMP物质的量比0.02∶1,反应时间1 h,反应温度128℃,FAME与TMP物质的量比4∶1,反应压力为300 Pa,在此条件下,脂肪酸甲酯的转化率为91.78%,反应混合物中TFATE质量分数为90.11%。经分子蒸馏分离纯化后终产品TFATE质量分数为97.02%。 | 汪勇 王尔佩 唐书泽 张震 Martin J T Reaney | 2013 | 中国粮油学报2013，28，2： | 8 |
| 4 | 乙醇萃取-HPLC法快速分析亚麻籽油中四种环肽研究显示文摘本文旨在利用乙醇快速富集亚麻籽油环肽,以期替代柱层析富集环肽法。在常温下,95%乙醇与亚麻籽油以料液比(g/mL)为5∶8震荡混合5 min,随后,将其在6 000 r/min下离心5 min,重复富集3次,收集上层清液并在40℃旋蒸,除去溶剂,用甲醇定容,根据HPLC外标法建立标准曲线,对环肽A、环肽C、环肽D和环肽E进行定量分析。通过加样回收试验可得,环肽C、环肽E、环肽D和环肽A的标准样品加样回收率分别为:99.5%、104.6%、109.7%、97.6%,相对标准偏差RSD分别为:1.4%,2.0%,2.5%和1.4%,证明了乙醇富集亚麻籽油环肽的方法准确可靠。此外,乙醇萃取法与传统的正相SPE硅胶柱分离法相比,操作简单,溶剂用量节省,富集效果更好。 | 利嘉祥 连莹君 张参 张宁 Martin Reaney 汪勇 | 2016 | 粮食与食品工业2016，23，2： | 8 |
| 5 | 亚麻籽环肽HPLC分析和氧化还原产物研究显示文摘对从天然亚麻籽中提取的粗环肽的组分和氧化还原性质进行探讨。采用HPLC测定亚麻籽粗环肽中环肽C(CLC)、环肽E(CLE)、环肽D(CLD)、环肽A(CLA)的含量,并通过还原的方法减少粗环肽中的环肽种类,以制备环肽单体。结果表明,环肽样品峰分离度良好,线性范围10~150μg/mL(环肽A的为10~100μg/mL),相关系数0.9990~0.9997。所测4种环肽的含量分别为CLC8.10%,CLE7.66%,CLD0.58%,CLA35.18%。粗环肽经过氧化或还原反应之后,其种类均发生改变。经HPLC定性分析,氧化后剩余6种环肽,还原后的环肽仅剩下4种,通过与已知峰对比,得知4种环肽分别为环肽B、环肽L、环肽A和环肽M,在C18分析柱上分离良好,以此为理论依据,采用Microsorb100-5C18制备柱(240mm×21.4mm),柱温30℃,流速5mL/min,70%乙腈溶液等度洗脱,在50min内可有效分离制备环肽单体。 | 左洋 陆学茸 滕英来 张宁 汪勇 REANEY Martin J T | 2015 | 中国油脂2015，40，8： | 4 |
| 6 | 产β-葡萄糖苷酶乳酸菌益生特性研究显示文摘研究了3株乳酸菌在不同碳源诱导下产β-葡萄糖苷酶的能力,并评价了其益生特性。研究发现,3株乳酸菌产生的β-葡萄糖苷酶同时具有水解烷基糖苷和芳香基糖苷的能力,纤维二糖诱导能显著提高β-葡萄糖苷酶活力。坚强肠球菌(Enterococcus durans)GW18275在纤维二糖为碳源时有最大β-葡萄糖苷酶活力(16.02 U/mL)。模拟胃肠液耐受试验结果表明,3株乳酸菌活菌数保持在10^(6) CFU/mL以上。植物乳植杆菌(Lactiplantibacillus plantarum)C5在3 g/L胆盐培养3 h有最大存活率(83.70±6.21)%,对二甲苯和乙酸乙酯的表面疏水性最佳。植物乳植杆菌(Lactiplantibacillus plantarum)C1有最高的自聚集率(80.72±1.58)%。此外,3株菌均无溶血性,对头孢菌素类、β-酰胺类及氨基糖苷类抗生素有不同的药敏性,对四环素均敏感。3株乳酸菌具有较高的β-葡萄糖苷酶活性,作为功能性益生菌候选物具有良好的益生特性,可为日后开发高产β-葡萄糖苷酶益生制剂提供基础。 | 冯程程 蔡子哲 陈琼 欧仕益 谢小冬 汪勇 MARTIN J T REANEY 张宁 | 2022 | 食品与发酵工业2022，48，15： | 4 |
| 7 | Diets enriched in trans -11 vaccenic acid alleviate ectopic lipid accumulation in a rat model of NAFLD and metabolic syndrome显示文摘 | M. Miriam Jacome-Sosa Faye Borthwick Rabban Mangat Richard Uwiera Martin J. Reaney Jianheng Shen Ariel D. Quiroga René L. Jacobs Richard Lehner Spencer D. Proctor | 2014 | The Journal of Nutritional Biochemistry2014，，： | 2 |
| 8 | Recent progress in the thermal treatment of oilseeds and oil oxidative stability:A review显示文摘Oxidative deterioration of vegetable oils is of great importance in the food industry.In China,vegetable oils produced via thermal pretreatment are popular owing to their strong oil flavor and enhanced yield.Here,we review:(i)the currently employed thermal treatment methods of oilseeds before oil extraction;(ii)effects of thermal treatments on the physicochemical properties,contents of minor lipid components,and oxidative stability of vegetable oils;and(iii)Maillard model systems that are related to oil and oilseed chemistry.Among the thermal pretreatment technologies,microwave and infrared radiations are promising,but these are not performed on the same large production scales as roasting.For most oilseeds,thermal treatments increase the yield of extracted oil and content of minor lipid compounds in the oil,such as polyphenols,tocopherols,and phytosterols.In addition,some Maillard reaction products(MRPs)generated by heating oilseeds have been extracted.The presence of both minor lipids and MRPs in the oil confers improved oxidative stability.However,the mechanism or relationship between thermal treatment and oxidative stability is yet to be clearly elucidated because vegetable oil oxidation is dependent on variables such as unsaturation,concentration and types of minor lipid components,MRPs,and the potential synergistic effects of these components.Recently,several Maillard reaction models related to thermally treated oilseeds have been established,suggesting that MRPs play a critical role during oxidation.However,comprehensive identification of antioxidants and the mechanism by which they inhibit oxidation are lacking.Future research can be performed to establish models that would help elucidate the antioxidative mechanisms of MRPs for more oilseeds.Using these models,it will be possible to predict the oil quality after processing,based on the presence of MRPs and oil chemistry. | Zizhe Cai Keyao Li Wan Jun Lee Martin T J Reaney Ning Zhang Yong Wang | 2021 | Fundamental Research2021，1，6： | 1 |
| 9 | The intestinal bioavailability of vaccenic acid and activation of peroxisome proliferator‐activated receptor‐α and ‐γ in a rodent model of dyslipidemia and the metabolic syndrome显示文摘 | Ye Wang Maria M. Jacome‐Sosa Megan R. Ruth Yan Lu Jianheng Shen Martin J. Reaney Shannon L. Scott Michael E. R. Dugan Hope D. Anderson Catherine J. Field Spencer D. Proctor Donna F. Vine | 2012 | Mol. Nutr. Food Res2012，，8： | 1 |
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