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| 1 | Bcl-x_L and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells显示文摘AIM: To explore the role of Bcl-xL and Myeloid cell leukaemia (Mcl)-1 for the apoptosis resistance of colorectal carcinoma (CRC) cells towards current treat-ment modalities. METHODS: Bcl-xL and Mcl-1 mRNA and protein ex-pression were analyzed in CRC cell lines as well as human CRC tissue by Western blot,quantitative PCRand immunohistochemistry. Bcl-xL and Mcl-1 protein expression was knocked down or increased in CRC cell lines by applying specific siRNAs or expression plas-mids,respectively. After modulation of protein expres-sion,CRC cells were treated with chemotherapeutic agents,an antagonistic epidermal growth factor recep-tor (EGFR1) antibody,an EGFR1 tyrosine kinase inhibi-tor,or with the death receptor ligand TRAIL. Apoptosis induction and cell viability were analyzed. RESULTS: Here we show that in human CRC tis-sue and various CRC cell lines both Bcl-xL and Mcl-1 are expressed. Bcl-xL expression was higher in CRC tissue than in surrounding non-malignant tissue,both on protein and mRNA level. Mcl-1 mRNA expression was significantly lower in ma-lignant tissues. However,protein expression was slightly higher. Viability rates of CRC cells were significantly decreased after knock down of Bcl-xL expression,and,to a lower extent,after knock down of Mcl-1 expression. Furthermore,cells with reduced Bcl-xL or Mcl-1 expression was more sensitive towards oxaliplatin-and irinotecan-induced apoptosis,and in the case of Bcl-xL also towards 5-FU-induced apoptosis. On the other hand,upregulation of Bcl-xL by transfec-tion of an expression plasmid decreased chemothera-peutic drug-induced apoptosis. EGF treatment clearly induced Bcl-xL and Mcl-1 expression in CRC cells. Apop-tosis induction upon EGFR1 blockage by cetuximab or PD168393 was increased by inhibiting Mcl-1 and Bcl-xL expression. More strikingly,CD95-and TRAIL-induced apoptosis was increased by Bcl-xL knock down. CONCLUSION: Our data suggest that Bcl-xL and,to a lower extent,Mcl-1,are important anti-apoptotic factors in CRC. Specific downregulation of Bcl-xL is a promising approach to sensitize CRC cells towards chemotherapy and targeted therapy. | Henning Schulze-Bergkamen Roland Ehrenberg Lothar Hickmann Binje Vick Toni Urbanik Christoph C Schimanski Martin R Berger Arno Schad Achim Weber Steffen Heeger Peter R Galle Markus Moehler | 2008 | World Journal of Gastroenterology2008，14，24： | 4 |
| 2 | Obesity in general elective surgery显示文摘 | Daniel Dindo Markus K Muller Markus Weber Pierre-Alain Clavien | 2003 | The Lancet . 2003 (9374)2003，，9374： | 2 |
| 3 | Overconfidence and trading volume显示文摘 | Markus Glaser Martin Weber | 2007 | THE GENEVA RISK AND INSURANCE REVIEW2007，，1： | 2 |
| 4 | Cost-effectiveness of FDG-PET for the management of potentially operable non-small cell lung cancer: priority for a PET-based strategy after nodal-negative CT results显示文摘 | Markus Dietlein Kerstin Weber Afschin Gandjour Detlef Moka Peter Theissen Karl W. Lauterbach Harald Schicha | 2000 | European Journal of Nuclear Medicine2000，，11： | 1 |
| 5 | Health-Related Quality of Life and Psychological Adjustment of Children and Adolescents with Hypospadias显示文摘 | Verena B. Sch?nbucher Markus A. Landolt Rita Gobet Daniel M. Weber | 2008 | The Journal of Pediatrics2008，，6： | 1 |
| 6 | Obesity in general elective surgery显示文摘 | Daniel Dindo Markus K Muller Markus Weber Pierre-Alain Clavien | 2003 | The Lancet2003，，9374： | 1 |
| 7 | 测定血清S100蛋白用于诊断克-雅病:前瞻性病例对照研究显示文摘目的:分析克-雅病(Creutzfeldt-Jakob dis-ease,CJD)患者及对照组血清中脑特异性 S100蛋白浓度。设计:前瞻性病例对照研究。研究单位:国家 CJD 监测站。研究对象:224例疑为 CJD 的转诊患者及35例无痴呆的对照病例。主要结果测定:CJD 患者、其他疾病引起的痴呆患者及对照组血清 S100浓度。结果:224例疑为 CJD 的患者中,65例经病理证实确诊;6例有基因突变而肯定诊断;43例很可能患 CJD;36例可能患 CJD;74例患有其他疾病。108例为肯定或很可能 CJD 患者,其血清 S100浓度中位数为395 pg/ml(SD 387 pg/ml)。这明显高于患其他疾病的74例患者(S100浓度中位数为109pg/ml,SDl77pg/ml;P=0.0001)。以213pg/ml为界值,S100测定用于 CJD 诊断的敏感性为77.8%(95%的可信区间为66.8%~85.2%),特异性为81.1%(70.3%~89.3%)。在 Kaplan-Meier 曲线上,不同浓度 S100患者的存活时间差异有显著性(P=0.023)。结论:S100血清浓度测定是一种有价值的工具,在 CJD 的鉴别诊断中,较测定脑脊液更有价值。尚需更多的研究以确定连续测定是否有助于提高此病的诊断准确性。 | Markus Otto Jens Wiltfang Ekkehard Schtz Ingo Zerr Anke Otto Annette Pfahlberg Olaf Gefeller Manfred Uhr Armin Giese Thomas Weber Hans A Kretzschmar Sigrid Poser 张星虎 | 1998 | 英国医学杂志中文版1998，0，1： | 1 |
| 8 | Faster subgraph isomorphism detection by well-founded total indexing显示文摘 | Markus Weber Marc as Liwicki Andreas Dengel | 2012 | Pattern Recognition Letters2012，33，15： | 1 |
| 9 | The modulation of Amyotrophic Lateral Sclerosis risk by Ataxin-2 intermediate polyglutamine expansions is a specific effect显示文摘 | Suzana Gispert Alexander Kurz Stefan Waibel Peter Bauer Inga Liepelt Christof Geisen Aaron D. Gitler Tim Becker Markus Weber Daniela Berg Peter M. Andersen Rejko Krüger Olaf Riess Albert C. Ludolph Georg Auburger | 2011 | Neurobiology of Disease2011，，1： | 1 |
| 10 | FDG PET imaging of locally advanced gastric carcinomas: correlation with endoscopic and histopathological findings显示文摘 | Alexander Stahl Katja Ott Wolfgang Weber Karen Becker Thomas Link J?rg-Rüdiger Siewert Markus Schwaiger Ulrich Fink | 2003 | European Journal of Nuclear Medicine and Molecular Imaging2003，，2： | 1 |
| 11 | O-(2-[18F]Fluoroethyl)-l-tyrosine and l-[methyl-11C]methionine uptake in brain tumours: initial results of a comparative study显示文摘 | Wolfgang A. Weber Hans-Jürgen Wester Anca L. Grosu Michael Herz Brigitte Dzewas Horst-Jürgen Feldmann Michael Molls Gerhard St?cklin Markus Schwaiger | 2000 | European Journal of Nuclear Medicine2000，，5： | 1 |
| 12 | FDG PET imaging of locally advanced gastric carcinomas: correlation with endoscopic and histopathological findings显示文摘 | Alexander Stahl Katja Ott Wolfgang Weber Karen Becker Thomas Link J?rg-Rüdiger Siewert Markus Schwaiger Ulrich Fink | 2003 | European Journal of Nuclear Medicine and Molecular Imaging2003，，2： | 1 |
| 13 | Cyclic AMP blocks bacterial lipopolysaccharide-induced myosin light chain phospho- rylation in endithelial ceils through inhibition of Rho/Rho kinase signaling 显示文摘 | Markus E James JM Weber PC | 2000 | J Immnnol2000，164，12： | 1 |
| 14 | Overconfidence and trading volume显示文摘 | Markus Glaser Martin Weber | 2007 | THE GENEVA RISK AND INSURANCE REVIEW2007，，1： | 1 |
| 15 | Heat stress response in plants: a complex game with chaperones and more than twenty heat stress transcription factors显示文摘 | Sanjeev Kumar Baniwal Kapil Bharti Kwan Yu Chan Markus Fauth Arnab Ganguli Sachin Kotak Shravan Kumar Mishra Lutz Nover Markus Port Klaus-Dieter Scharf Joanna Tripp Christian Weber Dirk Zielinski Pascal Koskull-D?ring | 2004 | Journal of Biosciences2004，，4： | 1 |
| 16 | Laparoscopic Pouch Resizing and Redo of Gastro-jejunal Anastomosis for Pouch Dilatation following Gastric Bypass显示文摘 | Markus K Müller Stefan Wildi Thomas Scholz Pierre-Alain Clavien Markus Weber | 2005 | Obesity Surgery2005，，8： | 1 |
| 17 | Preoperative Evaluation of Pancreatic Masses with Positron Emission Tomography Using 18F-fluorodeoxyglucose: Diagnostic Limitations显示文摘 | Andreas Sendler Norbert Avril Hermann Helmberger Jens Stollfu? Wolfgang Weber Frank Bengel Markus Schwaiger Jürgen D. Roder J. Rüdiger Siewert | 2000 | World Journal of Surgery2000，，9： | 1 |
| 18 | Laparoscopic Versus Open Splenectomy for Nontraumatic Diseases显示文摘 | Christine F. Maurus Markus Sch?fer Markus K. Müller Pierre-Alain Clavien Markus Weber | 2008 | World Journal of Surgery2008，，11： | 1 |
| 19 | Daclizumab induction therapy in combination with tacrolimus显示文摘 | Markus BH Weber S Yarque J | 2001 | Transplant Proc2001，33，12： | 1 |
| 20 | Mammotome: Less Invasive than ABBI with Similar Accuracy for Early Breast Cancer Detection显示文摘 | Walter P. Weber Rosanna Zanetti Igor Langer Sophie Dellas Markus Zuber Holger Moch Eugenia Remmel Daniel Oertli Edward Wight Walter R. Marti | 2005 | World Journal of Surgery2005，，： | 1 |
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