|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Sodium glucose co-transporter 2 inhibition reduces succinate levels in diabetic mice显示文摘BACKGROUND Type 1 diabetes(T1D) is associated with major chronic microvascular complications which contribute significantly to diabetes associated morbidity.The protein primarily responsible for glucose reabsorption in the kidney is sodium glucose co-transporter 2(SGLT2). Presently, SGLT2 inhibitors are widely used in diabetic patients to improve blood glucose levels and prevent cardiovascular and renal complications. Given the broad therapeutic application of SGLT2 inhibitors, we hypothesised that SGLT2 inhibition may exert its protective effects via alterations of the gut microbiome and tested this in a type 1 diabetic mouse model of diabetic retinopathy.AIM To determine whether the treatment with two independent SGLT2 inhibitors affects gut health in a type 1 diabetic mouse model.METHODS The SGLT2 inhibitors empagliflozin or dapagliflozin(25 mg/kg/d) or vehicle dimethylsulfoxide(DMSO) were administered to C57 BL/6 J, Akita, Kimba and Akimba mice at 10 wk of age for 8 wk via their drinking water. Serum samples were collected and the concentration of succinate and the short chain fatty acid(SCFA) butyric acid was measured using gas chromatography-mass spectrometry. Enzyme-linked immunosorbent assay(ELISA) was performed to determine the concentration of insulin and leptin. Furthermore, the norepinephrine content in kidney tissue was determined using ELISA. Pancreatic tissue was collected and stained with haematoxylin and eosin and analysed using brightfield microscopy.RESULTS Due to the presence of the Akita allele, both Akita and Akimba mice showed a reduction in insulin production compared to C57 BL/6 J and Kimba mice.Furthermore, Akita mice also showed the presence of apoptotic bodies within the pancreatic islets. The acinar cells of Akita and Akimba mice showed swelling which is indicative of acute injury or pancreatitis. After 8 wk of SGLT2 inhibition with dapagliflozin, the intermediate metabolite of gut metabolism known as succinate was significantly reduced in Akimba mice when compared to DMSO treated mice. In addition, empagliflozin resulted in suppression of succinate levels in Akimba mice. The beneficial SCFA known as butyric acid was significantly increased in Akita mice after treatment with dapagliflozin when compared to vehicle treated mice. The norepinephrine content in the kidney was significantly reduced with both dapagliflozin and empagliflozin therapy in Akita mice and was significantly reduced in Akimba mice treated with empagliflozin.In non-diabetic C57 BL/6 J and Kimba mice, serum leptin levels were significantly reduced after dapagliflozin therapy.CONCLUSION The inhibition of SGLT2 reduces the intermediate metabolite succinate, increases SCFA butyric acid levels and reduces norepinephrine content in mouse models of T1 D. Collectively, these improvements may represent an important mechanism underlying the potential benefits of SGLT2 inhibition in T1 D and its complications. | Lakshini Y Herat Natalie C Ward Aaron L Magno Elizabeth P Rakoczy Marcio G Kiuchi Markus P Schlaich Vance B Matthews | 2020 | World Journal of Gastroenterology2020，26，23： | 3 |
| 2 | Endoscopic ultrasound-guided fiducial marker placement in pancreatic cancer:A systematic review and meta-analysis显示文摘BACKGROUND Pancreatic cancer(PC)mortality remains high despite advances in therapy.Combination chemoradiotherapy offers modest survival benefit over monotherapy with either.Fiducial markers serve as needed landmarks for imageguided radiotherapy(IGRT).Traditionally,these markers were placed surgically or percutaneously with limitations of each.Endoscopic ultrasound-guided placement overcomes these limitations.AIM To evaluate the safety,efficacy,and feasibility of endoscopic ultrasound(EUS)-guided fiducial placement for PC undergoing IGRT.METHODS Articles were searched in MEDLINE,PubMed,and Ovid journals.Pooling was conducted by fixed and random effects models.Heterogeneity was assessed using Cochran’s Q test based upon inverse variance weights.RESULTS Initial search identified 1024 reference articles for EUS-guided fiducial placement in PC.Of these,261 relevant articles were reviewed.Data was extracted from 11 studies(n=820)meeting inclusion criteria.Pooled proportion of successful placement was 96.27%(95%CI:95.35-97.81)with fiducial migration rates low at 4.33%(95%CI:2.45-6.71).Adverse event rates remained low,with overall pooled proportion of 4.85%(95%CI:3.04-7.03).CONCLUSION EUS-guided placement of fiducial markers for IGRT of PC is safe,feasible,and efficacious.The ability to target deep structures under direct visualization while remaining minimally invasive are added benefits.Moreover,the ability to perform fine needle aspiration or celiac plexus neurolysis add value and increase patient-care efficiency.Whether EUS-guided fiducial placement improves outcomes in IGRT or offers any mortality benefits over traditional placement remains unknown and future studies are needed. | Jaymon B Patel Vakya Revanur David G Forcione Matthew L Bechtold Srinivas R Puli | 2020 | World Journal of Gastrointestinal Endoscopy2020，12，8： | 2 |
| 3 | Cloning and mapping of variety-speeific rice genomic DNA sequences:amplifled fragment length polymorphisms(AFLP)from silver-stained Polyaerylamide gels显示文摘 | Yong G C MattheW w B OliVier P et a1 | 1996 | Genome1996，39，： | 1 |
| 4 | Adaptation of HIV-1to human leukocyte antigen class I显示文摘 | Kawashima Y Pfafferott K Frater J Matthews P Payne R Addo M Gatanaga H Fujiwara M Hachiya A Koizumi H Kuse N Oka S Duda A Prendergast A Crawford H Leslie A Brumme Z Brumme C Allen T Brander C Kaslow R Tang J Hunter E Allen S Mulenga J Branch S Roach T John M Mallal S Ogwu A Shapiro R Prado J G Fidler S Weber J Pybus O G Klenerman P Ndung'u T Phillips R Heckerman D Harrigan P R Walker B D Takiguchi M Goulder P | 2009 | Nature2009，458，7238： | 1 |
| 5 | A system for Canadian dental readiness in the air force reserve显示文摘 | Callison G M Matthews B | 1998 | Mil Med1998，163，4： | 1 |
| 6 | Extraction and characterisation of lipids from Antarctic krill (Euphausia superba)显示文摘 | JOSEPH C G MATTHEW P D SARAH K B | 2010 | Food Chemistry2010，125，3： | 1 |
| 7 | Transparent conducting Nnc oxide thin films doped with aluminum and molybdenum显示文摘 | JOEL N D TIMOTHY A G DAVID M W TERESA M B MATTHEW Y BOBBY T TIMOTHY J C | 2007 | Journal V-acuum Society Technology2007，25，4： | 1 |
| 8 | Bleeding complications following percutaneous tendoachilles tenotomy in the treatment of clubfoot deformity显示文摘 | Matthew B Dobbs J Eric G | 2004 | J Pediatr Othop2004，24，4： | 1 |
| 9 | Caspase-9, caspase-3 and easpase-7 have distinct roles during in- trinsic apoptosis显示文摘 | MATTHEW B LUIS R M REBEKA L D G | 2013 | BMC Cell Biology2013，，14： | 1 |
| 10 | Rapid evolution of animal mitochondrial DNA显示文摘 | Wesley M B Matthew G JR Allen C W | 1979 | Proc Natl Acad Sci USA1979，76，4： | 1 |
| 11 | The effect of neoprene shorts on leg proprioception in Australian football players显示文摘 | MATTHEW L CAMERON ROGER D ADAMS CHRIS G MAHER B | 2008 | Journal of Science and Medicine in Sport2008，11，： | 1 |
| 12 | The layered and historical nature of ecological systems and the risk assessment ofpesticides显示文摘 | Landis W G Matthews R A Matthews G B | 1996 | Environ Toxicol Chem1996，15，： | 1 |
| 13 | Strepto- myces competition and co-evolution in relation to plant disease suppression 显示文摘 | Linda L K Daniel C S Matthew G B | 2012 | Research in Microbiology2012，163，8： | 1 |
| 14 | Extraction and characterization of lipids from Antarctic krill(Euphausia superba)显示文摘 | Joseph C G Matthew P D Sarah K B | 2011 | Food Chemistry2011，，125： | 1 |
| 15 | Development of an on-time logger for irrigation systems显示文摘 | Leib B G Matthews G Kroeger M | 2003 | Agricultural Water Management2003，62，1： | 1 |
| 16 | The community conditioning hypothesis and its application to environmentaltoxicology显示文摘 | Matthews R A Landis W G Matthews G B | 1996 | Environ Toxicol Chem1996，15，： | 1 |
| 17 | Evaluating uncertainty introduced to process-based simulation model estimates by alternative sources of meteorological data显示文摘 | Rivington M Matthews K B Bellocchi G | 2006 | Agricultural Systems2006，88，2： | 1 |
| 18 | Soybean development and yield as affected by three postemergence herbicides显示文摘 | Young B G Young J M Matthews J L | 2003 | Agron J2003，95，5： | 1 |
| 19 | Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array 显示文摘 | Wang SC Debbie W Kerrie F Alexandra A Shiaoman C Bevan EH Marco M Silvio S Sara GM Luigi C Anna MM Alex W Stuart S Gary B Ralf W Joerg P International Wheat Genome Sequencing Consortium Morten L Diane M Rudi A Rudy D Gina BG Abraham K Alina RA Catherine F Jerome S Michele M Curtis P Luo MC Jan DMM Jorge D Martin G Roberto T Cindy L Ivan M Colin C Keith JE Matthew H Eduard A | 2014 | Plant Biotechnol J2014，12，： | 1 |
| 20 | Risk factors affecting operating approach,conversion,and morbidity for adrenalectomy:a single institusion series of 402patients显示文摘 | Bittner J G 4th Gershuni V M Matthews B D | 2013 | Surg Endosc2013，7，27： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员