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| 1 | IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity显示文摘Obesity and associated metabolic diseases are characterized by a chronic low-grade inflammatory state with the infiltration of many inflammatory cells,especially macrophages.Immune molecules,including some cytokines,have a close relationship with metabolism.Interleukin(IL)-25 is a member of the IL-17 cytokine family that can regulate macrophages and alleviate some metabolic dysfunction;however,its role and mechanisms in lipid metabolism remain to be extensively clarified.Human serum and liver biopsy specimens,high-fat diet-induced obesity mice and DB/DB(Lepr−/−)animal models were used to examine IL-25 expression in obesity and nonalcoholic fatty liver diseases(NAFLD).To observe the role of IL-25 in lipid metabolism,model mice were administered with IL-25 or adoptively transferred with IL-25-educated macrophages in vivo,whereas bone marrow-derived macrophages,the macrophage cell line RAW264.7 and adipocytes differentiated from 3T3-L1 were used in vitro.IL-25 was decreased in NAFLD patients and obese mice.In addition,IL-25 reduced body weight gain and lipid accumulation,enhanced lipid uptake by macrophages and increased the expression of lipolysis andβ-oxidation enzymes via alternatively activating macrophages.IL-25 also promoted lipolysis and suppressed lipogenesis in adipocytes co-cultured with the IL-25-educated macrophages.Furthermore,IL-25 improved the mitochondrial respiratory capacity and oxygen consumption rate of macrophages and produced more NAD+/NADH and ATP.In conclusion,IL-25 can stimulate M2 macrophage polarization and thereby promote lipolysis and mitochondrial respiratory capacity,highlighting the potential for IL-25 to be used as a therapeutic agent against obesity and associated metabolic syndromes. | Juan Feng Lingyi Li Zhiying Ou Qiao Li Baoyong Gong Zhenxian Zhao Weiwei Qi Ti Zhou Jun Zhong Weibin Cai Xia Yang Aiping Zhao Guoquan Gao Zhonghan Yang | 2018 | Cellular & Molecular Immunology2018，15，5： | 10 |
| 2 | Defined tumor antigen-specific T cells potentiate personalized TCR-T cell therapy and prediction of immunotherapy response显示文摘Personalized immunotherapy targeting tumor-specific antigens(TSAs)could generate efficient and safe antitumor immune response without damaging normal tissues.Although neoantigen vaccines have shown therapeutic effect in clinic trials,precise prediction of neoantigens from tumor mutations is still challenging.The host antitumor immune response selects and activates T cells recognizing tumor antigens.Hence,T cells engineered with T-cell receptors(TCRs)from these naturally occurring tumor antigen-specific T(Tas)cells in a patient will target personal TSAs in his/her tumor.To establish such a personalized TCR-T cell therapy,we comprehensively characterized T cells in tumor and its adjacent tissues by single-cell mRNA sequencing(scRNA-seq),TCR sequencing(TCR-seq)and in vitro neoantigen stimulation.Compared to bystander T cells circulating among tissues,Tas cells were characterized by tumor enrichment,tumor-specific clonal expansion and neoantigen specificity.We found that CXCL13 is a unique marker for both CD4^(+)and CD8^(+)Tas cells.Importantly,TCR-T cells expressing TCRs from Tas cells showed significant therapeutic effects on autologous patient-derived xenograft(PDX)tumors.Intratumoral Tas cell levels measured by CXCL13 expression precisely predicted the response to immune checkpoint blockade,indicating a critical role of Tas cells in the antitumor immunity.We further identified CD200 and ENTPD1 as surface markers for CD4^(^(+))and CD8^(^(+))Tas cells respectively,which enabled the isolation of Tas cells from tumor by Fluorescence Activating Cell Sorter(FACS)sorting.Overall,our results suggest that TCR-T cells engineered with Tas TCRs are a promising agent for personalized immunotherapy,and intratumoral Tas cell levels determine the response to immunotherapy. | Jingjing He Xinxin Xiong Han Yang Dandan Li Xuefei Liu Shuo Li Shuangye Liao Siyu Chen Xizhi Wen Kuai Yu Lingyi Fu Xingjun Dong Kaiyu Zhu Xiaojun Xia Tiebang Kang Chaochao Bian Xiang Li Haiping Liu Peirong Ding Xiaoshi Zhang Zhenjiang Liu Wende Li Zhixiang Zuo Penghui Zhou | 2022 | Cell Research2022，32，6： | 10 |
| 3 | Overexpression of Hdac6 enhances resistance to virus infection in embryonic stem cells and in mice显示文摘亲爱的编辑, | Dekun Wang Qingwen Meng Lihong Huo Meng Yang Lingling Wang Xinyu Chen JianchaoWang Zhiguo Li Xiaoying Ye Na Liu Qiuyan Li Zhen Dai Hongsheng Ouyang Ning Li Jun Zhou Lingyi Chen Lin Liu | 2015 | Protein & Cell2015，6，2： | 3 |
| 4 | Overexpression of Hdac6 extends reproductive lifespan in mice显示文摘 | Xiaoxi Zhang Jiao Yang Haiying Wang Renpeng Guo Yu Yin Dongdong Zhang Qian Zhang Hua Wang Zhongcheng Zhou Lingyi Chen Jun Zhou Lin Liu | 2017 | Protein & Cell2017，8，5： | 1 |
| 5 | Brassinosteroid and gibberellin coordinate rice seed germination and embryo growth by regulating glutelin mobilization显示文摘Seed germination is the beginning of a new lifecycle,and involves many complex physiological and biochemical reactions including seed reserve mobilization in the endosperm and nutrient transport and reuse in the embryo.Although glutelin is a dominant storage protein in rice,its contribution to seed germination and its regulatory mechanisms are mostly unknown.Gibberellin (GA) and brassinosteroid (BR),two major growth-promoting phytohormones,also play positive roles in controlling seed germination.However,how GA and BR interact and coordinate seed germination and facilitate glutelin mobilization remains unclear.In the present study,biochemical and physiological analyses of seed germination indicated that both GA and BR promote seed germination and post-germination growth.Exogenous application of GA restored germination defects caused by BR deficiency or insensitivity.Proteomic and q RT-PCR results showed that the expression of several glutelin proteins and their encoding genes was induced by BR and GA in the embryo.Expression assays suggested that the increased accumulation of glutelin protein in the embryo was due to the accelerated degradation of glutelin by a cysteine proteinase (REP-1) in the endosperm.The breakdown of glutelin in the endosperm showed a strict positive correspondence with the length of the shoot.The GluA2 mutation led to reduced degradation rate of glutelin and defects in seed germination,and the promotion effect of GA on seed germination was weakened in the glua2mutant.In vitro culture assay of rice embryos showed that glutelin mobilization functioned downstream of the GA and BR pathways to promote shoot elongation.These findings suggest a mechanism that mediates crosstalk between BR and GA in co-regulating rice seed germination and embryo growth. | Min Xiong Lingyi Chu Qianfeng Li Jiawen Yu Yihao Yang Peng Zhou Yong Zhou Changquan Zhang Xiaolei Fan Dongsheng Zhao Changjie Yan Qiaoquan Liu | 2021 | The Crop Journal2021，9，5： | 1 |
| 6 | Lupeol, a Dietary Triterpene, Inhibited Growth, and Induced Apoptosis Through Down-Regulation of DR3 in SMMC7721 Cells显示文摘 | Lin Zhang Youcheng Zhang Lingyi Zhang Xiaojun Yang Zhicheng Lv | 2009 | Cancer Investigation2009，，2： | 1 |
| 7 | Nitric oxide synthase as a postharvest response in pathogen resistance of tomato fruit显示文摘 | Yang Zheng Lin Shen Mengmeng Yu Bei Fan Danying Zhao Lingyi Liu Jiping Sheng | 2010 | Postharvest Biology and Technology2010，，1： | 1 |
| 8 | Transparent conducting Sn-doped Ga1.4In0.6O3 films prepared on α-Al 2 O 3 (0<ce:hsp sp='0.25'/>0<ce:hsp sp='0.25'/>0<ce:hsp sp='0.25'/>1) by MOCVD显示文摘 | Fan Yang Jin Ma Caina Luan Lingyi Kong | 2009 | Applied Surface Science2009，，11： | 1 |
| 9 | Efficient CO_(2)Reduction to Formate on CsPbI_(3) Nanocrystals Wrapped with Reduced Graphene Oxide显示文摘Transformation of greenhouse gas(CO_(2))into valuable chemicals and fuels is a promising route to address the global issues of climate change and the energy crisis.Metal halide perovskite catalysts have shown their potential in promoting CO_(2)reduction reaction(CO_(2)RR),however,their low phase stability has limited their application perspective.Herein,we present a reduced graphene oxide(rGO)wrapped CsPbI_3 perovskite nanocrystal(NC)CO_(2)RR catalyst(CsPbI_3/rGO),demonstrating enhanced stability in the aqueous electrolyte.The CsPbI_3/rGO catalyst exhibited>92%Faradaic efficiency toward formate production at a CO_(2)RR current density of~12.7 mA cm^(-2).Comprehensive characterizations revealed the superior performance of the CsPbI_3/rGO catalyst originated from the synergistic effects between the CsPbI_3 NCs and rGO,i.e.,rGO stabilized theα-CsPbI_3 phase and tuned the charge distribution,thus lowered the energy barrier for the protonation process and the formation of~*HCOO intermediate,which resulted in high CO_(2)RR selectivity toward formate.This work shows a promising strategy to rationally design robust metal halide perovskites for achieving efficient CO_(2)RR toward valuable fuels. | Minh Tam Hoang Chen Han Zhipeng Ma Xin Mao Yang Yang Sepideh Sadat Madani Paul Shaw Yongchao Yang Lingyi Peng Cui Ying Toe Jian Pan Rose Amal Aijun Du Tuquabo Tesfamichael Zhaojun Han Hongxia Wang | 2023 | Nano-Micro Letters2023，15，10： | 1 |
| 10 | A Rat Model of Autologous Oral Mucosal Epithelial Transplantation for Corneal Limbal Stem Cell Failure显示文摘Purpose: To establish an animal model of autologous oral mucosa grafting for limbal stem cell deficiency.Methods: The study was carried from August to October2012. Fourteen SD rats were randomly and evenly allocated to study group A and control group B. Limbal stem cell deficiency was established by alkali burn in the right eye of each rat in both groups. Rats in group A received autologous oral mucosa strip transplantation following the chemical burn. Rats in group B did not receive surgery after the chemical burn.Topical antibiotics and dexamethasone were used in all rats.Corneal clarity, corneal fluorescein staining, oral mucosal graft survival, and complications at postoperative days 1,3,7,14 were observed.Results: The oral mucosa strip graft was detached in one rat in group A. Reepithelialization was observed starting from the graft position and was completed within 14 days in the remaining 6 eyes in group A. However, persistent corneal epithelium defect was observed in all eyes in group B, among which corneal melting and perforation was observed in 2 eyes and corneal opacification with neovascularization was observed in the remaining 5 eyes.Conclusion: Autologous oral mucosa strip grafting for limbal stem cell deficiency can be achieved by a rat model following chemical burn. The fate of the transplanted oral mucosal epithelial cells warrants further study. | Weihua Li Qiaoli Li Wencong Wang Kaijing Li Shiqi Ling Yuanzhe Yang Lingyi Liang | 2014 | Eye Science2014，29，1： | 0 |
| 11 | Clubroot resistance introgression in interspecific hybrids between Raphanus sativus and Brassica napus显示文摘Clubroot is a prevailing soil-borne disease affecting rapeseed production worldwide.However,few clubroot resistant rapeseed accessions were available for breeding.Identification and introgression of new clubroot resistant genes from closely related species by distant hybridization is an effective strategy.In the present study,9 radish(Raphanus sativus L.,2n=18,RR)lines resistant to Plasmodiophora brassicae pathotype 4 were used as donors to transfer clubroot resistance into a susceptible rapeseed(Brassica napus L.,2n=38,AACC)line by distant hybridization combined with embryo rescue.Nine intergeneric crosses were made but only 1(411×93039)produced F1 plants both from embryo rescue and natural seed-setting.Authenticity of triploid F1 hybrids(2n=28,ACR)were verified by flower color,cytological observation and molecular marker analysis,and 2 genuine F1 hybrids were identified.After chromosome doubling,these synthetic allohexaploid plants(2n=56,AACCRR)became partially fertile(pollen viability rate=35%)and were backcrossed with rapeseed parent to generate a BC1 population(2n=47,AACCR).Totally 178 BC1 plants were obtained,of which the majority(96.1%)were resistant to clubroot.These backcrossing progenies could be used for the breeding of new rapeseed varieties resistant to clubroot. | Xiuzhen Wang Lingyi Zeng Li Xu Wang Chen Fan Liu Huan Yang Peng Chi Li Ren Ruibin Yan Guangyuan Lu Xiaoping Fang | 2019 | Oil Crop Science2019，4，3： | 0 |
| 12 | The regulatory roles of DDIT4 in TDCIPP-induced autophagy and apoptosis in PC12 cells显示文摘Tris(1,3-dichloro-2-propyl) phosphate(TDCIPP) is a commonly used organophosphatebased flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce autophagy.However, its regulatory mechanism remains unclear. In this study, we found that the expression upregulation of the DNA Damage-Inducible Transcript 4(DDIT4) protein, which might play essential roles in TDCIPP-induced neuronal autophagy and apoptosis, was observed in TDCIPP-treated differentiated rat PC12 cells. Furthermore, we determined the protective effect of the DDIT4 suppression on the autophagy and apoptosis induced by TDCIPP using Western blot(WB) and Flow cytometry(FACS) analysis. We observed that TDCIPP treatment increased the DDIT4, the autophagy marker Beclin-1, and the microtubule-associated protein light chain 3-II(LC_(3)II) expressions and decreased the mTOR phosphorylation levels. Conversely, the suppression of DDIT4 expression increased the p-mTOR expression and decreased cell autophagy and apoptosis. Collectively, our results revealed the function of DDIT4 in cell death mechanisms triggered by TDCIPP through the m TOR signaling axis in differentiated PC12 cells. Thus, this study provided vital evidence necessary to explain the mechanism of TDCIPP-induced neurotoxicity in differentiated PC12 cells. | Li Li Lingyi Xi JinWu Zunquan Zhao Youliang Chen Weili Liu Zhihui Pan Mingzhu Liu Danfeng Yang Zhaoli Chen Yanjun Fang | 2023 | Journal of Environmental Sciences2023，，3： | 0 |
| 13 | Photochemical cycloaddition and temperature-dependent breathing in pillared-layer metal–organic frameworks显示文摘Single crystallinity of metal–organic frameworks(MOFs) enables the studies of their flexible behaviors with atomic precision. Here, we investigated the structural transformations triggered by photochemical cycloaddition and with temperature-dependent breathing in a series of pillared-layer MOF structures using a variety of pyrazolecarboxylate linkers for the layers and bipyridyl linkers as the pillars. The ethylenic double bonds from the pillars in close proximity undergo quantitative and seteroselective photochemical [2 + 2] cycloaddition upon UV irradiation, transforming the MOFs into structures with cyclobutane-based pillars. Furthermore, reversible breathing of the new pillared-layer MOF was evidenced by the 10.8% unit cell parameter change along c axis upon temperature change between 298 and 173 K. As revealed by single crystal X-ray diffraction, this transformation originates from the relative flattening of the wavy layers upon cooling. These two different types of characteristic structural transformations responding to inherent reactions and external stimuli happen at single crystalline state, providing a well-defined robust system with controlled flexibility. | Qingqing Pang Binbin Tu Lingyi Yang Qiaowei Li | 2019 | Science Bulletin2019，64，24： | 0 |
| 14 | Optical fluence compensation for handheld photoacoustic probe:An in vivo human study case显示文摘Integrating photoacoustic(PA)and ultrasound(US)into a handheld probe to perform P A/US dual-modal imaging has been widely studied over the past few years.However,optical fuence decreases quickly in deeper tissue due to light scattering and absorption,which would signifi-cantly affect the quantitative PA imaging.In this paper,we performed a fluence compensation for a PA imaging study of human breast.The comparison of P A/US image with and without optical fuence compensation demonstrated that the fuence compensation could effectively improve imaging quality for handheld probe. | Lingyi Zhao Meng Yang Yuxin Jiang Changhui Li | 2017 | Journal of Innovative Optical Health Sciences2017，，4： | 0 |
| 15 | Neuroprotective effects of repetitive transcranial magnetic stimulation on Alzheimer's disease: Undetermined therapeutic protocols and mechanisms显示文摘Alzheimer's disease(AD)is a prevalent neurodegenerative disorder characterized by gradual deterioration of cognitive functions,for which an effective treatment is currently unavailable.Repetitive transcranial magnetic stimulation(rTMS),a well-established noninvasive brain stimulation method,is utilized in clinical settings to address various neuropsychiatric conditions,such as depression,neuropathic pain,and poststroke dys-function.Increasing evidence suggests that rTMs may enhance cognitive abilities in individuals with AD.However,its optimal therapeutic protocols and precise mechanisms are currently unknown,impeding its clinical implementation.In the present review,we aimed to summarize and discuss the efficacy-related parameters in rTMs treatment,encompassing stimulus frequency,stimulus pattern,stimulus intensity,and the configuration of the stimulus coil.Furthermore,we reviewed promising rTMS therapeutic protocols involving various combinations of these factors,that were examined in clinical studies.Based on our analysis,we propose that a multisite high-frequency rTMS(HF-rTMS)regimen has value in AD therapy,and that promising single-site protocols,such as HF-rTMS,applied over the left dorsolateral prefrontal cortex,precuneus,or cerebellum are required to be validated in larger clinical studies.Lastly,we provide a comprehensive review of the potential mechanisms underlying the neuroprotective effects of rTMS on cognition in AD in terms of brain network modulation as well as cellular and molecular reactions.In conclusion,the interaction of diverse mechanisms may be responsible for the total therapeutic effect of rTMS on AD.This review provides theoretical and practical evidence for the future clinical application and scientific research of rTMS in AD. | Yang Zhu Lingyi Liao Shihao Gao Yong Tao Hao Huang Xiangqin Fang Changyan Yuan Changyue Gao | 2024 | Neuroprotection2024，2，1： | 0 |
| 16 | Multidisciplinary team for the diagnosis and treatment of 2cases of primary intestinal yolk sac tumor显示文摘Extragonadal primary yolk sac tumor of the intestinal tract origin is exceedingly rare. Through a multiple disciplinary team, the diagnosis and treatment of primary intestinal yolk sac tumor were further defined. We report 2 such cases with detailed histologic and immunohistochemical analysis. The two patients were a 7-year-old girl and a 29-year-old woman. Both of them preoperatively had an elevated serum alpha fetoprotein(AFP) level(≥ 1,210 ng/mL). The tumors are located in the intestine and imaging examination indicated the rectum as the primary site. Grossly the mass was grey-white and crisp texture. Microscopic examination featured reticular, microcystic, macrocystic, papillary, solid, and some glandular patterns. Immunohistochemically,tumor cells of both cases were positive for SALL4, AFP, pan-cytokeratin(AE1/AE3), and glypican-3. Simultaneously, a stain for EMA, OCT4, CD30, HCG, vimentin and CK20 were negative in all 2 neoplasms. The features of morphology,immunohistochemistry, laboratory examinations and imaging studies consist of the diagnosis of primary yolk sac tumor of the intestine. | Qiujuan Huang Changxu Liu Ruijun Tang Jie Li Wei Li Lei Zhu Yuhong Guo Lin Zhang Tongyuan Qu Lingyi Yang Lingmei Li Yalei Wang Baocun Sun Bin Meng Jie Yan Wengui Xu Huilai Zhang Dengfeng Cao Wenfeng Cao | 2018 | Cancer Biology & Medicine2018，15，4： | 0 |
| 17 | Bioactive A-ring rearranged limonoids from the root barks of Walsura robusta显示文摘Screening active natural products, rapid identification, and accurate isolation are of great important for modern natural lead compounds discovery1. We hereby reported the isolation of seven new neotecleanin-type limonoids(1–7), seven new limonoids with 5-oxatricyclo[5.4.0.11,4]hendecane ring system(8–14), and two new precursors(15–16) together with four known limonoids(17–20) from the root barks of Walsura robusta. Their structures, including their absolute configurations, were elucidated based on analyses of HR-ESI-MS, 1D/2D NMR, ECD spectrum calculations and singlecrystal X-ray diffraction techniques. Compounds 2, 8, 9, 11, 13, 14, 18 showed significant anti-inflammatory activities in LPS-induced RAW 264.7 cell line, BV2 microglial cells, and Propionibacterium acnes-stimulated THP-1 human monocytic cells. Walrobsin M(11) exhibited anti-inflammatory activity with IC50 value of 7.9670.36 μmol/L, and down-regulated phosphorylation levels of ERK and p38 in a dose-dependent manner. | Faliang An Xiaobing Wang Minghua Yang Jun Luo Lingyi Kong | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 0 |
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