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| 1 | Predictive factors associated with gefitinib response in patients with advanced non-small-cell lung cancer(NSCLC)显示文摘Purpose: A number of different clinical characteristics have been reported to singly correlate with therapeutic activity of epidermal growth factor receptor(EGFR) tyrosine kinase inhibitors(TKIs) in advanced non-small-cell lung cancer(NSCLC). This study aimed to identify predictive factors associated with prognostic benefits of gefitinib.Patients and methods: EGFR gene typing in 33 advanced NSCLC patients received gefitinib(250 mg/day) were analyzed with mutant-enriched PCR assay. Gefitinib response was evaluated with potential predictive factors retrospectively.Results: The overall objective response rate(ORR) and median progression-free survival(PFS) in the 33 patients treated by gefitinib were 45.5% and 3.0(2.0-4.0) months. The ORR and median PFS in EGFR gene mutation patients were significantly higher/longer than those in EGFR gene wild-type patients(P<0.01). Similarly, the ORR and median PFS in non-smoker patients were significantly higher/longer than those in smoker patients(P<0.05, P<0.01, respectively). However, no difference for ORR and median PFS occurred between male and female patients. Logistic multivariate analysis showed that only EGFR mutated gene was significantly associated with the ORR(P<0.01). Both EGFR mutated gene and non-smoker were the major factors that contributed to PFS(P<0.05). Conclusions: EGFR mutated gene and non-smoker status are potential predictors for gefitinib response in NSCLC patients. | Lian Chen Rui Chen Zhe Zhu Yichen Zhang Zhengwei Wen Yun Li Xiaoming Li Yuwen Luo Liyu Ma Shuguang Lin Xin Chen | 2014 | Chinese Journal of Cancer Research2014，26，4： | 7 |
| 2 | Effect of amino acid mutation at position 127 in 3A of a rabbitattenuated foot-and-mouth disease virus serotype Asia1 on viral replication and infection显示文摘An amino acid mutation(R127→I) in the 3A non-structural protein of an FMDV serotype Asia1 rabbit-attenuated ZB strain was previously found after attenuation of the virus. To explore the effects of this mutation on viral replication and infection, the amino acid residue isoleucine(I) was changed to arginine(R) in the infectious cDNA clone of the rabbit-attenuated ZB strain by sitedirected mutagenesis, and the R127-mutated virus was rescued. BHK monolayer cells and suckling mice were inoculated with the R127-mutated virus to test its growth property and pathogenicity, respectively. The effects of the R127 mutation on viral replication and virulence were analyzed. The data showed that there was a slight difference in plaque morphology between the R127-mutated and wild-type viruses. The growth rate of the mutated virus was lower in BHK-21 cells and its virulence in suckling mice was also attenuated. This study indicates that the R127 mutation in 3A may play an important role in FMDV replication in vitro and in pathogenicity in suckling mice. | Aiguo Xin Mingwang Zhu Qi Hu Haisheng Miao Zhenqi Peng Yuwen He Lin Gao Huachun Li | 2014 | Virologica Sinica2014，29，5： | 2 |
| 3 | Characterization and Proteomic Analysis of Novel Rice Lesion Mimic Mutant with Enhanced Disease Resistance显示文摘Lesion mmic mutants(LMMs)are plants that spontaneously form lesions without pathogeninfection or external stimulus and exhibit resistance to pathogens.Here,a rice LMM was created by ethylmethane sulfonate mutagenesis,named as hpil(hydrogen peroxide induced lesion).Diaminobenzidineand trypan blue staining showed that large amounts of H_(2)O_(2) were produced and cell death was occurredat and around the parts of lesion mimic in the rice leaves.The phenotype of hpil is controlled by a singlerecessive gene,localized at a 2 Mb interval on chromosome 2.The data suggested that hpil is a novelLMM with enhanced bacterial and fungal disease resistance,and multiple pathogenesis-related proteins(PRs)were up-regulated.The proteomes of leaves at three positions(different degrees of lesion mimicseverity)were characterized in hpil compared with its wild type plant.Differentially expressed proteinswere detected by two dimensional difference gel electrophoresis and 274 proteins were identified byMALDITOF/TOFTM.These proteins were related to metabolic process,cellular process and response tostimulus,with mostly down-regulated in hpil leaves.Many of these proteins were related to the Calvincycle,photosynthetic electron transport chain,glycolysis/gluconeogenesis and phosphonates pathways.Some resistance-related proteins including 14-3-3 proteins,OsPR10 and antioxidases such asperoxidase,superoxide dismutase and ascorbate peroxidase were up-regulated in leaves with lesionmimic.These results provide the foundation for cloning of the target gene and shed light on themechanism involved in autaimmunity of rice. | Yang Yong Lin Qiujun Chen Xinyu Liang Weifang Fu Yuwen Xu Zhengjin Wu Yuanhua Wang Xuming Zhou Jie Yu Chulang Yan Chengqi Mei Qiong Chen Jianping | 2021 | Rice science2021，28，5： | 2 |
| 4 | Anti-inflammatory activities of aqueous extract from Radix Ophiopogon japonicus and its two constituents 显示文摘 | Kou Junping Sun Yang Lin Yuwen | 2005 | Biol Pharm Bull2005，28，7： | 1 |
| 5 | Effect of rare earth elements on electrochemical properties of La-Mg-Ni-based hydrogen storage alloys 显示文摘 | Li Yuan Han Da Han Shumin Zhu Xilin Hu Lin Zhang Zhong Liu Yuwen | 2009 | International Journal of Hydrogen Energy2009，34，3： | 1 |
| 6 | Motion Vector Coding in the HEVC Standard 显示文摘 | Lin Jianliang Chen Yiwen Huang Yuwen | 2013 | IEEE Journal of Selected Topics in Signal Processing2013，7，6： | 1 |
| 7 | Effect of high pressure heat treatment on microstructure and corrosion resistance of brass 显示文摘 | Liu Yuwen Liu Lin Zhang Wei | 2011 | Advanced Materials Research2011，，： | 1 |
| 8 | Motion vector coding in the HEVC standard显示文摘 | LIN Jianliang CHEN Yiwen HUANG Yuwen | 2013 | IEEE Journal of Selected Topics in Signal Processing2013，6，7： | 1 |
| 9 | Characteristics of Ti-MCM-41 and its catalytic properties in oxidation of benzene 显示文摘 | Lin Hsinyu | 2002 | Journal of Porous Materials2002，9，： | 1 |
| 10 | 显示文摘 | Lin Hsinyu Chen Yuehfang Chen Yuwen | 2007 | International Journal of Hydrogen Energy2007，32，1： | 1 |
| 11 | Magnetic Resonance Imaging and DWI Features of Orbital Rhabdomyosarcoma显示文摘Purpose: To describe the magnetic resonance imaging(MRI)features of orbital rhabdomyosarcoma(RMS).Methods: Thirty-nine patients with histopathologically confirmed orbital RMS were retrospectively reviewed. All patients underwent orbital conventional MRI, including axial, sagittal,and coronal T1-weighted, T2-weighted, and postcontrast T1-weighted sequences. The location, shape, margin, and MRI signal of the 39 lesions were reviewed. DWI in 15 patients and susceptibility weighted imaging(SWI) in 2 patients were also analyzed.Results: Orbital MRI was available in 39 patients and revealed a soft tissue mass in the orbital region in all cases. Of the 39 patients, the primary tumor sites were limited to the orbital proper in 31 cases, while 28 cases had extraocular muscle invasion and 8 cases had extraorbital invasion. All lesions were unilateral. Thirty-three cases were well-defined soft tissue masses and 6 cases appeared as less well-defined softtissue masses. Thirty-four cases showed homogeneous isointense or slightly hypointense signals on T1-weighted imaging(T1WI) and hyperintense signal on T2-weighted imaging(T2WI) compared with extraocular muscles. Five cases had heterogeneous signals with focal areas of increased signal on T1WI or decreased signal on T2WI, including 1 case with hypointense signal on SWI. The mean apparent diffusion coefficient(ADC) value of the viable part of tumors was(0.925±0.09)×10-3mm2/ s. All cases showed moderate to marked enhancement after contrast administration.Conclusion: Several MRI features-including homogeneous isointense or slightly hypointense signal on T1WI and slightly hyperintense signal on T2WI, relative low ADC values, and moderate to marked enhancement, extraocular muscles invasion, and extraorbital extensionare helpful in the diagnosis of orbital RMS. | Xuetao Mu Hong Wang Yueyue Li Yuwen Hao Chunnan Wu Lin Ma | 2014 | Eye Science2014，29，1： | 0 |
| 12 | Antioxidant procyanidin B2 protects oocytes against cryoinjuries via mitochondria regulated cortical tension显示文摘Background:Irreversible cryodamage caused by oocyte vitrification limited its wild application in female fertility preservation.Antioxidants were always used to antagonist the oxidative stress caused by vitrification.However,the comprehensive mechanism underlying the protective role of antioxidants has not been studied.Procyanidin B2(PCB2)is a potent natural antioxidant and its functions in response to vitrification are still unknown.In this study,the effects of PCB2 on vitrified-thawed oocytes and subsequent embryo development were explored,and the mechanisms underlying the protective role of PCB2 were systematically elucidated.Results:Vitrification induced a marked decline in oocyte quality,while PCB2 could improve oocyte viability and further development after parthenogenetic activation.A subsequent study indicated that PCB2 effectively attenuated vitrification-induced oxidative stress,rescued mitochondrial dysfunction,and improved cell viability.Moreover,PCB2 also acts as a cortical tension regulator apart from strong antioxidant properties.Increased cortical tension caused by PCB2 would maintain normal spindle morphology and promote migration,ensure correct meiosis progression and finally reduce the aneuploidy rate in vitrified oocytes.Further study reveals that ATP biosynthesis plays a crucial role in cortical tension regulation,and PCB2 effectively increased the cortical tension through the electron transfer chain pathway.Additionally,PCB2 would elevate the cortical tension in embryo cells at morula and blastocyst stages and further improve blastocyst quality.What's more,targeted metabolomics shows that PCB2 has a beneficial effect on blastocyst formation by mediating saccharides and amino acids metabolism.Conclusions:Antioxidant PCB2 exhibits multi-protective roles in response to vitrification stimuli through mitochondria-mediated cortical tension regulation. | Qingrui Zhuan Jun Li Xingzhu Du Luyao Zhang Lin Meng Yuwen Luo Dan Zhou Hongyu Liu Pengcheng Wan Yunpeng Hou Xiangwei Fu | 2023 | Journal of Animal Science and Biotechnology2023，14，1： | 0 |
| 13 | Problems and Countermeasures to Improve Clinical Trial Participants’Compensation Rights in China显示文摘Objective To discuss the problems existing in the compensation of Chinese clinical trial participants and propose some suggestions for improving their rights.Methods The literature related to the participants’right to compensation at home and abroad was searched to study the inadequacy of the compensation right for clinical trial participants in China from four aspects:insurance system,principle of attribution,legal relationship and compensation regulations.Then,some suggestions to improve the participants’right to compensation were proposed.Results and Conclusion China lacks clear legal norms for participants’right to compensation.There are problems such as unclear insurance rules and compensation rules,unclear contractual relationships between parties to clinical trials,and no laws and regulations to rely on for attribution and compensation.China should issue regulatory guidelines related to the right to compensation of participants in clinical trials,so that all parties in clinical trials can have rules to follow if there is the occurrence of injury,which can better protect the rights and interests of the participants. | Yang Xiuqiao Wen Ping Lin Hong Chen Wenhuan Chen Yuwen | 2024 | Asian Journal of Social Pharmacy2024，19，1： | 0 |
| 14 | Performance Comparison of Computational Methods for the Prediction of the Function and Pathogenicity of Non-coding Variants显示文摘Non-coding variants in the human genome significantly influence human traits and complex diseases via their regulation and modification effects.Hence,an increasing number of computational methods are developed to predict the effects of variants in human non-coding sequences.However,it is difficult for inexperienced users to select appropriate computational methods from dozens of available methods.To solve this issue,we assessed 12 performance metrics of 24 methods on four independent non-coding variant benchmark datasets:(1)rare germline variants from clinical relevant sequence variants(ClinVar),(2)rare somatic variants from Catalogue Of Somatic Mutations In Cancer(COSMIC),(3)common regulatory variants from curated expression quantitative trait locus(eQTL)data,and(4)disease-associated common variants from curated genomewide association studies(GWAS).All 24 tested methods performed differently under various conditions,indicating varying strengths and weaknesses under different scenarios.Importantly,the performance of existing methods was acceptable for rare germline variants from ClinVar with the area under the receiver operating characteristic curve(AUROC)of 0.4481–0.8033 and poor for rare somatic variants from COSMIC(AUROC=0.4984–0.7131),common regulatory variants from curated eQTL data(AUROC=0.4837–0.6472),and disease-associated common variants from curated GWAS(AUROC=0.4766–0.5188).We also compared the prediction performance of 24 methods for non-coding de novo mutations in autism spectrum disorder,and found that the combined annotation-dependent depletion(CADD)and context-dependent tolerance score(CDTS)methods showed better performance.Summarily,we assessed the performance of 24 computational methods under diverse scenarios,providing preliminary advice for proper tool selection and guiding the development of new techniques in interpreting non-coding variants. | Zheng Wang Guihu Zhao Bin Li Zhenghuan Fang Qian Chen Xiaomeng Wang Tengfei Luo Yijing Wang Qiao Zhou Kuokuo Li Lu Xia Yi Zhang Xun Zhou Hongxu Pan Yuwen Zhao Yige Wang Lin Wang Jifeng Guo Beisha Tang Kun Xia Jinchen Li | 2023 | Genomics, Proteomics & Bioinformatics2023，21，3： | 0 |
| 15 | Investigation of transfer ionization processes in the collision of partially stripped carbon ions on Argon显示文摘The ratios of the cross section of the transfer-ionization to the single-electron-capture of Argon induced by Cq+ (q=1,2,3) ions are measured by means of position sensitive and time-of-flight techniques. Our experimental results are compared with the data of Heq+ (q=1,2)-Ar of DuBois. A qualitative interpretation is presented based on the Classical-Over-Barrier Model of Bohr. | WANG Jun CHEN XiMeng DING BaoWei FU HongBin CUI Ying SHAO JianXiong LU YanXia GAO ZhiMin LIU YuWen Du Juan SUN GuangZhi CHEN Lin | 2008 | Science China(Physics,Mechanics & Astronomy)2008，51，7： | 0 |
| 16 | Single-cell transcriptome analysis uncovers underlying mechanisms of acute liver injury induced by tripterygium glycosides tablet in mice显示文摘Tripterygium glycosides tablet(TGT),the classical commercial drug of Tripterygium wilfordii Hook.F.has been effectively used in the treatment of rheumatoid arthritis,nephrotic syndrome,leprosy,Behcet's syndrome,leprosy reaction and autoimmune hepatitis.However,due to its narrow and limited treatment window,TGT-induced organ toxicity(among which liver injury accounts for about 40%of clinical reports)has gained increasing attention.The present study aimed to clarify the cellular and molecular events underlying TGT-induced acute liver injury using single-cell RNA sequencing(scRNA-seq)technology.The TGT-induced acute liver injury mouse model was constructed through short-term TGT exposure and further verified by hematoxylin-eosin staining and liver function-related serum indicators,including alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase and total bilirubin.Using the mouse model,we identified 15 specific subtypes of cells in the liver tissue,including endothelial cells,hepatocytes,cholangiocytes,and hepatic stellate cells.Further analysis indicated that TGT caused a significant inflammatory response in liver endothelial cells at different spatial locations;led to marked inflammatory response,apoptosis and fatty acid metabolism dysfunction in hepatocytes;activated hepatic stellate cells;brought about the activation,inflammation,and phagocytosis of liver capsular macrophages cells;resulted in immune dysfunction of liver lymphocytes;disturbed the intercellular crosstalk in liver microenvironment by regulating various signaling pathways.Thus,these findings elaborate the mechanism underlying TGT-induced acute liver injury,provide new insights into the safe and rational applications in the clinic,and complement the identification of new biomarkers and therapeutic targets for liver protection. | Qiuyan Guo Jiangpeng Wu Qixin Wang Yuwen Huang Lin Chen Jie Gong Maobo Du Guangqing Cheng Tianming Lu Minghong Zhao Yuan Zhao Chong Qiu Fei Xia Junzhe Zhang Jiayun Chen Feng Qiu Jigang Wang | 2023 | Journal of Pharmaceutical Analysis2023，13，8： | 0 |
| 17 | Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms显示文摘Rheumatoid arthritis(RA)is a common chronic inflammatory disorder that usually affects joints.It was found that roburic acid(RBA),an ingredient from anti-RA herb Gentiana macrophylla Pall.,displayed strong anti-inflammatory activity.However,its medical application is limited by its hydrophobicity,lack of targeting capability and unclear functional mechanism.Here,we constructed a pH responsive dual-target drug delivery system hitchhiking RBA(RBA-NPs)that targeted both CD44 and folate receptors,and investigated its pharmacological mechanism.In rat RA model,the nanocarriers effectively delivered RBA to inflammatory sites and significantly enhanced the therapeutic outcomes compared with free RBA,as well as strongly reducing inflammatory cytokine levels and promoting tissue repair.Following analysis revealed that M1 macrophages in the joints were reprogrammed to M2 phenotype by RBA.Since the balance of pro-and anti-inflammatory macrophages play important roles in maintaining immune homeostasis and preventing excessive inflammation in RA,this reprogramming is likely responsible for the anti-RA effect.Furthermore,we revealed that RBA-NPs drove M1-to-M2 phenotypic switch by down-regulating the glycolysis level via blocking ERK/HIF-1α/GLUT1 pathway.Thus,our work not only developed a targeting delivery system that remarkably improved the anti-RA efficiency of RBA,but also identified a potential molecular target to reversely reprogram macrophages though energy metabolism regulation. | Na Jia Yunzhen Gao Min Li Yi Liang Yuwen Li Yunzhu Lin Shiqi Huang Qing Lin Xun Sun Qin He Yuqin Yao Ben Zhang Zhirong Zhang Ling Zhang | 2023 | Signal Transduction and Targeted Therapy2023，8，8： | 0 |
| 18 | The shadows hang over immunotherapy-neutrophil extracellular traps in cancer显示文摘The development of immune checkpoint inhibitors(ICIs)was a milestone in the advancement of anticancer treatment.ICIs exert their effects by enhancing the function of T cells via inhibition of immune checkpoints,such as programmed cell death protein-1(PD-1)/programmed cell death-ligand 1(PDL1). | Dan Pu Liyuan Yin Xiaoqian Zhai Ruoxiang Wang Lin Huang Qiang Wu Lingling Zhu Yuwen Zhou Qinghua Zhou Lu Li | 2023 | Science China(Life Sciences)2023，66，5： | 0 |
| 19 | 大规模无创产前检测的基因组分析揭示了多个性状的遗传关联、病毒的感染模式以及中国人群的遗传历史显示文摘文章简介本研究分析了参与无创产前基因检测(NIPT)的141,431名中国女性的全基因组低深度测序数据。研究团队使用这些数据描绘了中国人的群体遗传特征,并探索了遗传因素与母婴健康等性状的相互关联情况。 | Siyang Liu Shujia Huang Fang Chen Lijian Zhao Yuying Yuan Stephen Starko Francis Lin Fang Zilong Li Long Lin Rong Liu Yong Zhang Huixin Xu Shengkang Li Yuwen Zhou Robert W.Davies Qiang Liu Robin G.Walters Kuang Lin Jia Ju Thorfinn Korneliussen Melinda A.Yang Qiaomei Fu Jun Wang Lijun Zhou Anders Krogh Hongyun Zhang Wei Wang Zhengming Chen Zhiming Cai Ye Yin Huanming Yang Mao Mao Jay Shendure 汪建 Anders Albrechtsen 金鑫 Rasmus Nielsen 徐讯 | 2019 | 科学新闻2019，0，2： | 0 |
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