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| 1 | Cloning and soluble expression of mature α-luffin from Luffa cylindrica and its antitumor activities in vitro显示文摘Luffin -- 一,一种单个链的类型我使核糖体失去活性的蛋白质,它被知道是最 luffin 家庭并且显然有毒拥有 antitumor 活动,从 Luffa cylindrica 种子被孤立。在现在的学习,成熟 -luffin 从 L 被克隆。cylindrica 和它被发现那成熟 -luffin 分享有 luffin 的 96% 氨基酸类似 -- 一。recombinant 成熟 -luffin 成功地在表示条件的优化以后在 Escherichia coli 在一种部分可溶的形式被表示。细菌的生长上的 recombinant 蛋白质的效果并且它的在里面 vitro 蛋白质合成抑制活动被测试。然后,它对不同人的癌症房间线的 antitumor 活动被 CCK-8 试金和流动 cytometry 评估。结果显示 recombinant -luffin 对 E 稍微有毒。coli。它能在兔子网状细胞 lysate 系统禁止蛋白质合成。同时,它在一个剂量依赖者和时间依赖者举止禁止了肿瘤细胞线的生长。另外, recombinant -luffin 能由 apoptosis 导致房间死亡。向肿瘤房间的 -luffin 的 cytotoxicity 使它成为一个潜在的 antitumor 代理人。 | Liling Liu Rupeng Wang Wei He Fengtian He Gang Huang | 2010 | Acta Biochimica et Biophysica Sinica2010，42，8： | 6 |
| 2 | Aquaporin 5 is degraded by autophagy in diabetic submandibular gland显示文摘Autophagy is a catabolic process which is involved in the development of many diseases including diabetes mellitus and its complications. Hyposalivation is a common complication of diabetes mellitus, whereas its mechanism remains unclear. Here, we observed that the stimulated salivary flow rate of SMG was significantly decreased in db/db mice, a diabetic mice model. The expressions of aquaporin 5(AQP5), a water channel protein, were decreased, whereas the m RNA level of AQP5 was increased in SMGs of both diabetic patients and mice. Under transmission electron microcope, more autophagosomes were detected in diabetic SMGs. Expressions of autophagy related proteins LC3 II, Beclin-1 and ATG5 were increased, meanwhile autophagy substrate p62 was decreased in SMGs of diabetic patients and mice, indicating that autophagy was activated in diabetic SMG.Double immunofluorescence staining showed that the colocalization of AQP5 and LC3 was increased in SMGs of diabetic mice.In cultured SMG-C6 cells, high glucose(HG), but not high osmotic pressure, reduced AQP5 protein expression and induced autophagy. Moreover, inhibition of autophagy by 3-methyladenin, an autophagy inhibitor, or by autophagy-related gene 5 siRNA, decreased HG-induced AQP5 reduction in SMG-C6 cells. Additionally, the expression of p-p85, p-Akt and p-mTOR were decreased in HG-treated SMG-C6 cells. Pretreatment with 740 Y-P, a PI3 K agonist, significantly suppressed HG-induced autophagy and AQP5 degradation. Taken together, these results indicate that autophagy plays a crucial role in AQP5 degradation in diabetic SMG via PI3 K/Akt/mTOR signaling pathway, which contributes to the dysfunction of diabetic SMG. Our study provides a novel mechanism of diabetic hyposalivation. | Yan Huang Xijin Shi Qianying Mao Yan Zhang Xin Cong Xueming Zhang Zhejing Zhang Liling Wu Ruolan Xiang Guangyan Yu | 2018 | Science China(Life Sciences)2018，61，9： | 5 |
| 3 | The first strain of monkeypox isolated in the Chinese Mainland and preserved at the National Pathogen Resource Center of China显示文摘On September 16,2022,the first imported monkeypox case was reported in the Chinese Mainland.Laboratory tests including nucleic acid detection were carried out in Chongqing Nan’an Centre for Disease Control and Prevention and Chongqing Center for Disease Control and Prevention.After that,monkeypox virus was isolated by the Institute for Viral Disease Control and Prevention and preserved at the National Pathogen Resource Center on September 18,2022.The National Pathogen Resource Center shared the basic information of monkeypox virus strain,samples,biosafety,strain imaging,RT-PCR primers,and probes sequence timely to support the prevention and control of monkeypox epidemic and facilitate the scientific research on vaccine development,drug screening and evaluation of monkeypox virus in the future. | Qiang Wei Baoying Huang Wei Huang Jingdong Song Li Mei Li Zhao Jing Yin Juan Zhang Wenling Wang Fei Ye Caiyun Li Liling Bai Yanhai Wang Yao Deng Bo Liu Mengnan Jiang Guizhen Wu Jun Han Hua Zhao Hua Ling Wenjie Tan Jianjun Liu | 2022 | Infectious Medicine2022，1，4： | 1 |
| 4 | The Phytoremediation Potential of a Singapore Forest Tree for Bioretention Systems显示文摘 | Xiangting Cleo Chenl Liling Huang Bee Lian Ong | 2014 | 材料科学与工程(中英文A版)2014，4，7： | 1 |
| 5 | Functional α1-adrenoceptor subtypes in human submandibular gland显示文摘 | Huang Zan Wu Liling Zhang Youyi | 2006 | J Dent Res2006，85，： | 1 |
| 6 | Plant Traits for Phytoremediation in the Tropics显示文摘Water is a limited and valuable resource.Singapore has four national sources of water supply,one of which is natural precipitation.Pollutants collected in stormwater runoff are deposited into drainage systems and reservoirs.Major nutrient pollutants found in local stormwater runoff include nitrate and phosphate,which may cause eutrophication.Bioretention systems are efficient in removing these pollutants in the presence of plants.This paper discusses plant traits that can enhance the phytoremediation of nutrient pollutants in stormwater runoff for application in bioretention systems.The plant species studied showed variations in chlorophyll florescence,leaf greenness,biomass production,and nitrate and phosphate removal.In general,dry biomass was moderately correlated to nitrate and phosphate removal(r=0.339–0.501).Root,leaf,and total dry biomass of the native tree species showed a moderate to strong correlation with nitrate removal(r=0.811,0.657,and 0.727,respectively).Leaf dry biomass of fastgrowing plants also showed a moderate to strong relationship with the removal of both pollutants(r=0.707 and 0.609,respectively).Root dry biomass of slow-growing plants showed a strong relationship with phosphate removal(r=0.707),but the correlation was weaker for nitrate removal(r=0.557).These results are valuable for choosing plants for application in bioretention systems. | Xiangting Cleo Chen Liling Huang Tze Hsien Agnes Chang Bee Lian Ong Say Leong Ong Jiangyong Hu | 2019 | Engineering2019，5，5： | 1 |
| 7 | AF9YEATS结构域在分子层面偶联组蛋白巴豆酰化和活跃转录显示文摘染色质结合蛋白质模块(“阅读器”)识别组蛋白共价修饰构成表观遗传调控的主要方式之一,例如溴域识别乙酰化赖氨酸修饰。除乙酰化之外,近来研究发现组蛋白赖氨酸还能发生巴豆酰化、丁酰化、丙酰化等酰基化修饰.但能够偏好识别这些新型酰基化修饰的阅读器尚未见报道。 | Yuanyuan Li Benjamin R.Sabari Tatyana Panchenko Hong Wen Dan Zhao Haipeng Guan Liling Wan He Huang Zhanyun Tang Yingming Zhao Robert G.Roeder Xiaobing Shi C.David Allis 李海涛 | 2017 | 科学新闻2017，19，4： | 0 |
| 8 | Linguistic Characteristics of Mandarin-Speaking Huntington’s Disease Patients显示文摘Objective Linguistic problem is common in Huntington’s disease(HD)patients.It has been studied before in native speakers of alphabetic languages,such as English.As a hieroglyphic language,Chinese differs from alphabetic languages in terms of phonology,morphology,semantics and syntax.We aimed to investigate the linguistic characteristics of manifest HD in native speakers of Mandarin.Meanwhile,we expected to explore the linguistic differences associated with cortical or subcortical pathology.Methods Five HD patients and five Alzheimer’s disease(AD)patients matched in age,gender,disease course and educational level were enrolled.All the participants were Mandarin native speakers.All finished history inquiry,physical examination,basic test,genetic test and neuropsychological assessment.Language evaluation was performed by Aphasia Battery of Chinese.Results HD patients had a mean disease course of 5.4±2.97(range,2-10)years.They showed a linguistic disorder close to transcortical motor aphasia.They exhibited prominent phonological impairment,as well as slight semantic and syntactic abnormality.Tonic errors were found in speech.Character structural errors and substitutions were detected in writing.In comparison,AD patients showed a more severe linguistic impairment,characterized by global aphasia with more semantic errors.Conclusion Mandarin-speaking HD patients have a transcortical motor aphasia-like disturbance with prominent phonological impairment,whereas AD patients have a more severe global aphasia with salient semantic impairment. | Liling Dong Caiyan Liu Chenhui Mao Shanshan Chu Jie Li Xinying Huang Jing Gao | 2020 | Chinese Medical Sciences Journal2020，35，3： | 0 |
| 9 | CPAL, as a New Mediator of Cardiomyocyte Metabolic Alterations and Pyroptosis, Regulates Myocardial Infarction Injury in Mice显示文摘Myocardial infarction (MI), the most serious of the ischemic heart diseases, is accompanied by myocardial metabolic disorders and the loss of cardiomyocytes. Increasing evidence has shown that long noncoding RNAs (lncRNAs) are involved in various pathological conditions such as cancer and cardiovascular diseases (CVDs), and are emerging as a novel biomarker for these disorders. This study aims to investigate the regulatory role and mechanisms of lncRNAs in myocardial remodeling in the setting of MI. We find that post-infarcted hearts exhibit a reduction of adenosine triphosphate (ATP) and an alteration of the glucose and lipid metabolism genes cluster of differentiation 36 (CD36), hexokinase 1 (HK1), and clucose transporter 4 (GLUT4), accompanied by cardiomyocyte pyroptosis. We then identify a previously unknown conserved lncRNA, AK009126 (cardiomyocyte pyroptosis-associated lncRNA, CPAL), which is remarkably upregulated in the myocardial border zone of MI mice. Importantly, the adeno-associated virus 9 (AAV9)-mediated silencing of endogenous CPAL by its short hairpin RNA (shRNA) partially abrogates myocardial metabolic alterations and cardiomyocyte pyroptosis during MI in mice. Mechanistically, CPAL is shown to bind directly to nuclear factor kappa B (NFκB) and to act as an activator of NFκB to induce NFκB phosphorylation in cardiomyocytes. We also find that CPAL upregulates caspase-1 expression at the transcriptional level and consequently promotes the release of interleukin (IL)-18 and IL-1β from cardiomyocytes. Collectively, our findings reveal the conserved lncRNA CPAL as a new regulator of cardiac metabolic abnormalities and cardiomyocyte pyroptosis in the setting of MI and suggest CPAL as a new therapeutic target to protect cardiomyocytes against ischemic injury in infarcted hearts. | Jiamin Li Hongru Xue Ning Xu Liling Gong Ming Li Sijia Li Di Huang Qingwei Zhang Pengyu Li Qingsui Li Hang Yu Yining Liu Yadong Xue Haixin Chen Jiali Liu Wanyu Zhang Mingbin Liu Siyu Chang Xianzhi Lang Xingmiao Zhao Weijie Du Benzhi Cai Ning Wang Baofeng Yang | 2023 | Engineering2023，，1： | 0 |
| 10 | Dual inhibition of glucose uptake and energy supply synergistically restrains the growth and metastasis of breast cancer显示文摘Metastatic breast cancer(MBC) is one of the most common and knotty diseases in female population which could place them in a life-threatening condition. For malignant proliferation and migration, cancer cells require a large amount of glucose and energy to meet the demand of rapid metabolism. Hence,efficiently diminishing the utilization of energy substances by cancer cells is emerging as validated therapeutic strategies for cancer therapy. Herein, a nanoplatform with dual-inhibition of glucose uptake and oxidative phosphorylation(OXPHOS) was designed, which consisted of albendazole(ABZ) and atovaquone(ATO) by simple carrier-free self-assembling. The introduction of ABZ could evidently decrease glucose uptake to reduce the main “energy fuel” of cancer cells. Meanwhile, as a blocker of OXPHOS, ATO would reduce adenosine triphosphate(ATP) production and ameliorate hypoxia microenvironment by suppressing mitochondrial respiratory chain. Under such dual inhibition of energy metabolism, AA NPs exerted synergistic energy exhaustion effect and outstanding hypoxia improvement function, efficiently inhibiting tumor growth and metastasis. This research not only illustrates the feasibility of energy metabolism therapy by co-inhibiting glucose uptake and OXPHOS, but also provides an ingenious tactic to diminish metastasis during MBC treatment. | Yuan Xu Liling Huang Yuyang Bi Qi Song Mengmeng Zhang Lingfeng Zhang Tianjiao Zhou Lei Xing Hulin Jiang | 2023 | Chinese Chemical Letters2023，34，4： | 0 |
| 11 | Study progression in application of process analytical technologies on film coating显示文摘Film coating is an important unit operation to produce solid dosage forms,thereby,the monitoring of this process is helpful to find problems in time and improve the quality of coated products.Traditional methods adopted to monitor this process include measurement of coating weight gain,performance of disintegration and dissolution test,etc.However,not only do these methods cause destruction to the samples,but also consume time and energy.There have recently emerged the applications of process analytical technologies(PAT)on film coating,especially some novel spectroscopic and imaging technologies,which have the potential to real-time track the progress in film coating and optimize production efficiency.This article gives an overview on the application of such technologies for film coating,with the goal to provide a reference for the further researches. | Tingting Peng Ying Huang Liling Mei Linna Wu Longkai Chen Xin Pan Chuanbin Wu | 2015 | Asian Journal of Pharmaceutical Sciences2015，10，3： | 0 |
| 12 | Quantum phase transition and destruction of Kondo effect in pressurized SmB6显示文摘SmB_6 has been a well-known Kondo insulator for decades, but recently attracts extensive new attention as a candidate topological system. Studying SmB_6 under pressure provides an opportunity to acquire the much-needed understanding about the effect of electron correlations on both the metallic surface state and bulk insulating state. Here we do so by studying the evolution of two transport gaps(low temperature gap E_l and high temperature gap E_h) associated with the Kondo effect by measuring the electrical resistivity under high pressure and low temperature(0.3 K) conditions. We associate the gaps with the bulk Kondo hybridization, and from their evolution with pressure we demonstrate an insulator-tometal transition at ~4 GPa. At the transition pressure, a large change in the Hall number and a divergence tendency of the electron-electron scattering coefficient provide evidence for a destruction of the Kondo entanglement in the ground state. Our results raise the new prospect for studying topological electronic states in quantum critical materials settings. | yazhou zhou qi wu priscila f.s.rosa rong yu jing guo wei yi shan zhang zhe wang honghong wang shu cai ke yang aiguo li zheng jiang shuo zhang xiangjun wei yuying huang peijie sun yi-feng yang zachary fisk qimiao si zhongxian zhao liling sun | 2017 | Science Bulletin2017，62，21： | 0 |
| 13 | ASb(SO_(4))_(2)(A=Rb,Cs):Two short-wave UV antimony sulfates exhibiting large birefringence显示文摘Herein,two antimony sulfates,named RbSb(SO_(4))_(2)(1)and CsSb(SO_(4))_(2)(2),have been successfully synthesized with the introduction of Sb^(3+)cation with stereochemically active lone pairs(SCALP)into sulfates by the conventional hydrothermal method.Both two compounds endow short ultraviolet(UV)absorption edges(281 nm and 278 nm,respectively)and large birefringence(0.171@546 nm and 0.174@546 nm,respectively),which means that they are promising short-wave UV optical materials.Interestingly,though both of the two compounds exhibit similar 1D chained structures,and possess the same functional moieties including SbO4 seesaws and SO4 tetrahedral groups,they exhibit significantly opposite macroscopic symmetries,i.e.,compound 1 crystallizes in a centrosymmetric(CS)manner(P2_(1)/n)and compound 2 in a noncentrosymmetric(NCS)manner(P2_(1)2_(1)2_(1)),due to the size of cations[r(Rb+)=1.56 A˚,r(Cs+)=1.67 A˚]affects the orientation of SCALP of the adjacent Sb^(3+). | Yang Lan Jinxuan Ren Pu Zhang Xuehua Dong Ling Huang Liling Cao Daojiang Gao Guohong Zou | 2024 | Chinese Chemical Letters2024，35，1： | 0 |
| 14 | Serum Proteomics Profile of Renal Allograft Rejection by Combination of Magnetic Bead Sample Isolation and MALDI- TOF MS显示文摘 | Weiguo Sui Liling Huang Yong Dai Jiejing Chen Qiang Yan He Huang | 2009 | 器官移植内科学杂志2009，4，1： | 0 |
| 15 | Serum Proteomic Study of Patients with Antibody-Mediated Chronic Rejection显示文摘 | Weiguo Sui Qiang Yan liling Huang Yong Dai Jiejing Chen | 2013 | 器官移植内科学杂志2013，8，4： | 0 |
| 16 | 能量匮乏条件下Snf1-Mec1-Atg1通过控制线粒体呼吸作用调控细胞自噬的发生显示文摘文章简介m TOR和AMPK两个信号分子在细胞自噬的发生过程中扮演着非常重要的调控作用。近年来,人们对于m TOR信号分子如何调控细胞自噬的研究比较清楚,但是对于在能量匮乏条件下,AMPK信号分子如何调控细胞自噬的发生尚不清楚。 | Cong Yi Jingjing Tong Puzhong Lu Yizheng Wang Jinxie Zhang Chen Sun Kangning Yuan Renyu Xue Bing Zou Nianzhong Li Shuhua Xiao Chong Dai Yuwei Huang Liling Xu Lin Li She Chen Di Miao Haiteng Deng Hongliang Li 俞立 | 2018 | 科学新闻2018，0，4： | 0 |
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