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| 1 | Developmentally Regulated Glucosylation of Bitter Triterpenoid in Cucumber by the UDP-Glucosyltransferase UGT73AM3显示文摘 | Yang Zhong Xiaofeng Xue Zhiqiang Liu Yongshuo Ma Kewu Zeng Lida Han Jingjing Qi Dae-Kyun Ro Soren Bak Sanwen Huang Yuan Zhou Yi Shang | 2017 | Molecular Plant2017，10，7： | 7 |
| 2 | A structural and data-driven approach to engineering a plant cytochrome P450 enzyme显示文摘Functional manipulation of biosynthetic enzymes such as cytochrome P450 s(or P450 s) has attracted great interest in metabolic engineering of plant natural products.Cucurbitacins and mogrosides are plant triterpenoids that share the same backbone but display contrasting bioactivities.This structural and functional diversity of the two metabolites can be manipulated by engineering P450 s.However,the functional redesign of P450 s through directed evolution(DE) or structure-guided protein engineering is time consuming and challenging,often because of a lack of high-throughput screening methods and crystal structures of P450 s.In this study,we used an integrated approach combining computational protein design,evolutionary information,and experimental data-driven optimization to alter the substrate specificity of a multifunctional P450(CYP87 D20)from cucumber.After three rounds of iterative design and evaluation of 96 protein variants,CYP87 D20,which is involved in the cucurbitacin C biosynthetic pathway,was successfully transformed into a P450 mono-oxygenase that performs a single specific hydroxylation at C11 of cucurbitadienol.This integrated P450-engineering approach can be further applied to create a de novo pathway to produce mogrol,the precursor of the natural sweetener mogroside,or to alter the structural diversity of plant triterpenoids by functionally manipulating other P450 s. | Dawei Li Yongshuo Ma Yuan Zhou Junbo Gou Yang Zhong Lingling Zhao Lida Han Sergey Ovchinnikov Ling Ma Sanwen Huang Per Greisen Yi Shang | 2019 | Science China(Life Sciences)2019，62，7： | 5 |
| 3 | Optimisation of extraction conditions for polysaccharides from the roots of Isatis tinctoria L. by response surface methodology and their in vitro free radicals scavenging activities and effects on IL-4 and IFN-γ mRNA expression in chicken lymphocytes显示文摘 | Jiangwei Han Xingming Jiang Lida Zhang | 2011 | Carbohydrate Polymers2011，，3： | 1 |
| 4 | Plasma esterified and non-esterified fatty acids metabolic profiling using gas chromatography mass spectrometry and its application in the study of diabetic mellitus and diabetic nephropathy显示文摘 | Han LiDa Xia JianFei Liang QiongLin | 2011 | Anal Chim Acta2011，689，1： | 1 |
| 5 | Enhanced chemoselectivity of a plant cytochrome P450 through protein engineering of surface and catalytic residues显示文摘Cytochrome P450s(P450s)are the most versatile catalysts utilized by plants to produce structurally and functionally diverse metabolites.Given the high degree of gene redundancy and challenge to functionally characterize plant P450s,protein engineering is used as a complementarystrategy to study the mechanisms of P450-mediated reactions,or to alter their functions.We previously proposed an approach of engineering plant P450s based on combining high accuracy homology models generated by Rosetta combined with data-driven design using evoluti onary information of these enzymes.With this strategy,we repurposed a multi-functional P450(CYP87D20)into a monooxygenase after red esigning its active site.Since most plant P450s are membrane-anchored proteins that are adapted to the micro-environments of plant cells,expressing them in heterologous hosts usually results in problems of expression or activity.Here,we applied compu-tational design to tackle these issues by simultaneous optimization of the protein surface and active site.After screening 17 variants,effective su bstitutions of surface residues were observed to improve both expression and activity of CYP87D20.In addition,the identified substitutions were additive and by com-bining them a highly eficient C11 hydroxylase of cucurbitadienol was created to participate in the mogrol biosynthesis.This study shows the importance of considering the interplay between surface and active site residues for P450 engineering.Our integrated strategy also opens an avenue to create more tai loring enzymes with desired functions for the metabolic engineering of high-valued compounds like mogrol,the precursor of natural sweetener mogrosides. | Xiaopeng Zhang Wei Luo Yinying Yao Xuming Luo Chao Han Yang Zhong Bo Zhang Dawei Li Lida Han Sanwen Huang Per Greisen Yi Shang | 2021 | aBIOTECH2021，2，3： | 1 |
| 6 | Expression Profiles of 10,422 Genes at Early Stage of Low Nitrogen Stress in Rice Assayed using a cDNA Microarray显示文摘 | Xingming Lian Shiping Wang Jianwei Zhang Qi Feng Lida Zhang Danlin Fan Xianghua Li Dejun Yuan Bin Han Qifa Zhang | 2006 | Plant Molecular Biology2006，，5： | 1 |
| 7 | Quantitative analysis of collagen morphology in breast cancer from millimeter scale using multiphoton microscopy显示文摘The tumor microenvironment(TME)is now recognized as an important participant of tumor progression.As the most abundant extracellular matrix component in TME,collagen plays an important role in tumor development.The imaging study of collagen morphological feature in TME is of great significance for understanding the state of tumor.Multiphoton microscopy(MPM),based on second harmonic generation(SHG)and two-photon excitation fluorescence(TPEF),can be used to monitor the morphological changes of biological tissues without labeling.In this study,we used MPM for large-scale imaging of early invasive breast cancer from the tumor center to normal tissues far from the tumor.We found that there were signi¯cant di®erences in collagen morphology between breast cancer tumor boundary,near tumor transition region and normal tissues far from the tumor.Furthermore,the morphological feature of eight collagen¯bers was extracted to quantify the variation trend of collagen in three regions.These results may provide a new perspective for the optimal negative margin width of breast-conserving surgery and the understanding of tumor metastasis. | Yulan Liu Shunwu Xu Deyong Kang Xingxin Huang Shuoyu Xu Lianhuang Li Liqin Zheng Lida Qiu Zhenlin Zhan Xiahui Han Jianxin Chen | 2023 | Journal of Innovative Optical Health Sciences2023，16，4： | 0 |
| 8 | The domestication-associated L1 gene encodes a eucomic acid synthase pleiotropically modulating pod pigmentation and shattering in soybean显示文摘Pod coloration is a domestication-related trait in soybean,with modern cultivars typically displaying brown or tan pods,while their wild relative,Glycine soja,possesses black pods.However,the factors regulating this color variation remain unknown.In this study,we cloned and characterized L1,the classical locus responsible for black pods in soybean.By using map-based cloning and genetic analyses,we identified the causal gene of L1 and revealed that it encodes a hydroxymethylglutaryl-coenzyme A(CoA)lyase-like(HMGL-like)domain protein.Biochemical assays showed that L1 functions as a eucomic acid synthase and facilitates the synthesis of eucomic acid and piscidic acid,both of which contribute to coloration of pods and seed coats in soybean.Interestingly,we found that L1 plants are more prone to pod shattering under light exposure than l1 null mutants because dark pigmentation increases photothermal efficiency.Hence,pleiotropic effects of L1 on pod color and shattering,as well as seed pigmentation,likely contributed to the preference forl1 alleles during soybean domestication and improvement.Collectively,our study provides new insights into the mechanism of pod coloration and identifies a new target for future de novo domestication oflegume crops. | Xiangguang Lyu Ying-hui Li Yanfei Li Delin Li Chao Han Huilong Hong Yu Tian Lida Han Bin Liu Li-juan Qiu | 2023 | Molecular Plant2023，16，7： | 0 |
| 9 | Photocatalytic Decarboxylative Minisci Reaction Catalyzed by Palladium-Loaded Gallium Nitride显示文摘The decarboxylative Minisci reaction is a versatile tool for the direct C-H alkylation of heteroarenes,where stoichiometric amounts of oxidants or expensive,precious metal reagents are commonly used.Herein,we reported a photodriven decarboxylative Minisci reaction enabled by a gallium nitride-based heterogeneous photocatalyst under mild conditions.This method can be effectively applied to a broad substrate scope of acids,including primary,secondary,and tertiary carboxylic acids and N-heteroarenes effectively.The practicability and robustness of the approach are demonstrated for the functionalization of biologically active compounds. | Lida Tan Hyotaik Kang Mingxin Liu Hui Su Jing-Tan Han Chao-Jun Li | 2023 | Precision Chemistry2023，1，7： | 0 |
| 10 | Screening of Dominant Species of Triehogramma sp. and Evaluation of Field Control Effect显示文摘[Objective]The paper was to screen out the dominant species of Trichogramma sp.parasitized on Osirinia nub ilalis eggs and to evaluate the field control effect of Trichogramma sp..[Method]Six kinds of fresh egg masses of Trichogramma sp.parasitized on O.nubilalis were used to screen out three dominant species of Trichogramma sp.,and the single factor experimental design was used to evaluate field control effect.The dominant species were determined by combining yield,recovery yield loss rate and yield increase rate.[Result]T.osiriniae had the highest parasitic rate on O.nub ilalis;the parasitic rate of egg masses was 74.0%,and the parasitic rate of egg granules was 65.4%.The second was T.dendrolimi;the parasitic rate of egg masses was 62.0%,and the parasitic rate of egg granules was 59.5%.The parasitic rates of egg masses and egg granules of T.chilonis were 58.0%and 47.1%,respectively.The three Trichogramma sp.were the dominant bee species parasitized on O.nubilalis eggs.The average control effect of T.osirinae parasitized on O.nubilalis in the field was 73.86%;the average control effect of T.dendrolimi was 68.47%;and the average control effect of T.chilonis was 47.97%.The recovery yield loss rates was 9.58%,7.02%and 4.02%,and the yield increase rates were 9.65%,7.47%and 4.52%,respectively.[Conclusion]Considering the control effect and cost control comprehensively,T.dendrolimi should be used to control O.nubilalis in actual production. | Li Qingchao Wang Lida Zhao Xiumei Liu Yang Han Yehui Yang Ying Gao Chong | 2020 | Plant Diseases and Pests2020，11，3： | 0 |
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