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| 1 | Chemical compound cinobufotalin potently induces FOXO1-stimulated cisplatin sensitivity by antagonizing its binding partner MYH9显示文摘In this study,we present novel molecular mechanisms by which FOXO1 functions as a tumor suppressor to prevent the pathogenesis of nasopharyngeal carcinoma(NPC).First,we observed that FOXO1 not only controlled tumor stemness and metastasis,but also sensitized NPC cells to cisplatin(DDP)in vitro and in vivo.Mechanistic studies demonstrated that FOXO1-induced miR-200b expression through the GSK3β/β-catenin/TCF4 network-mediated stimulation of ZEB1,which reduced tumor stemness and the epithelial–mesenchymal transition(EMT)signal.Furthermore,we observed FOXO1 interaction with MYH9 and suppression of MYH9 expression by modulating the PI3K/AKT/c-Myc/P53/miR-133a-3p pathway.Decreased MYH9 expression not only reduced its interactions with GSK3β,but also attenuated TRAF6 expression,which then decreased the ubiquitin-mediated degradation of GSK3βprotein.Increased GSK3βexpression stimulated theβ-catenin/TCF4/ZEB1/miR-200b network,which increased the downstream tumor stemness and EMT signals.Subsequently,we observed that chemically synthesized cinobufotalin(CB)strongly increased FOXO1-induced DDP chemosensitivity by reducing MYH9 expression,and the reduction in MYH9 modulated GSK3β/β-catenin and its downstream tumor stemness and EMT signal in NPC.In clinical samples,the combination of low FOXO1 expression and high MYH9 expression indicated the worst overall survival rates.Our studies demonstrated that CB potently induced FOXO1-mediated DDP sensitivity by antagonizing its binding partner MYH9 to modulate tumor stemness in NPC. | YongHao Li Xiong Liu Xian Lin Menyang Zhao Yanyi Xiao Chen Liu Zixi Liang Zelong Lin Renhui Yi Zibo Tang Jiahao Liu Xin Li Qingping Jiang Libo Li Yinyin Xie Zhen Liu Weiyi Fang | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 6 |
| 2 | Simultaneous extraction of DNA and RNA from Escherichia coli BL 21 based on silica-coated magnetic nanoparticles显示文摘The extraction of nucleic acid is recognized as one of the most essential steps in molecular biology for initiating other downstream applications such as sequencing, amplification, hybridization, and cloning. Many commercial kits and methods are currently available that allow the extraction of only one type of nucleic acids-DNA or RNA. However, in parallel clinical detection of several diseases, a method for simultaneous extraction of both DNA and RNA from the same source is needed in such cases. In this study, a method for simultaneous extraction of DNA and RNA from bacteria based on magnetic nanoparticles(MNPs) was described. Lysis buffers were prepared to help the nucleic acid released and adsorbed to MNPs. Then, two washing buffers were used to remove the contamination of proteins and carbohydrates. The nucleic acids were finally eluted by Deoxyribonuclease(DNase) and Ribonucleases(RNase) free water. Different factors which might affect the purification of the nucleic acid were investigated, and the quantity and quality parameters of the nucleic acid were also recorded. The DNA and RNA extracted from bacteria were then respectively subjected to polymerase chain reaction(PCR) and reverse transcription PCR(RT-PCR) to further confirm its quality. The results indicated that our method can be successfully used to simultaneously extract DNA and RNA from bacteria. | Jiuhai Wang Zeeshan Ali Nianyue Wang Wenbiao Liang Hongna Liu Fu Li Haowen Yang Lei He Libo Nie Nongyue He Zhiyang Li | 2015 | Science China Chemistry2015，58，11： | 5 |
| 3 | The correlation between nitrogen species in coke and NO_x formation during regeneration显示文摘Nitrogen oxides(NO x)emission during the regeneration of coked fluid catalytic cracking(FCC)catalysts is an environmental issue.In order to identify the correlations between nitrogen species in coke and different nitrogencontaining products in tail gas,three coked catalysts with multilayer structural coke molecules were prepared in a fixed bed with model compounds(o-xylene and quinoline)at first.A series of characterization methods were used to analyze coke,including elemental analysis,FT-IR,XPS,and TG–MS.XPS characterization indicates all coked catalysts present two types of nitrogen species and the type with a higher binding energy is related with the inner part nitrogen atoms interacting with acid sites.Due to the stronger adsorption ability on acid sites for basic nitrogen compounds,the multilayer structural coke has unbalanced distribution of carbon and nitrogen atoms between the inner part and the outer edge,which strongly affects gas product formation.At the early stage of regeneration,oxidation starts from the outer edge and the product NO can be reduced to N_2 in high CO concentration.At the later stage,the inner part rich in nitrogen begins to be exposed to O_2.At this period,the formation of CO decreases due to lack of carbon atoms,which is not bene ficial to the reduction of NO.Therefore,nitrogen species in the inner part of multilayer structural coke contributes more to NO X formation.Based on the multilayer structure model of coke molecule and its oxidation behavior,a possible strategy to control NO X emission was discussed merely from concept. | Teng Li Chaohe Yang Xiaobo Chen Libo Yao Wei Liang Xuemei Ding | 2016 | Chinese Journal of Chemical Engineering2016，24，5： | 5 |
| 4 | Flexible Waterproof Piezoresistive Pressure Sensors with Wide Linear Working Range Based on Conductive Fabrics显示文摘Developing flexible sensors with high working performance holds intense interest for diverse applications in leveraging the Internet-of-things(IoT)infrastructures.For flexible piezoresistive sensors,traditionally most efforts are focused on tailoring the sensing materials to enhance the contact resistance variation for improving the sensitivity and working range,and it,however,remains challenging to simultaneously achieve flexible sensor with a linear working range over a high-pressure region(>100 kPa)and keep a reliable sensitivity.Herein,we devised a laserengraved silver-coated fabric as'soft'sensor electrode material to markedly advance the flexible sensor's linear working range to a level of 800 kPa with a high sensitivity of 6.4 kPa^-1 yet a fast response time of only 4 ms as well as long-time durability,which was rarely reported before.The integrated sensor successfully routed the wireless signal of pulse rate to the portable smartphone,further demonstrating its potential as a reliable electronic.Along with the rationally building the electrode instead of merely focusing on sensing materials capable of significantly improving the sensor's performance,we expect that this design concept and sensor system could potentially pave the way for developing more advanced wearable electronics in the future. | Hongcheng Xu Libo Gao Yuejiao Wang Ke Cao Xinkang Hu Liang Wang Meng Mu Min Liu Haiyan Zhang Weidong Wang Yang Lu | 2020 | Nano-Micro Letters2020，12，11： | 4 |
| 5 | Nano-sized Fe_2O_3/Fe_3O_4 facilitate anaerobic transformation of hexavalent chromium in soil–water systems显示文摘The purpose of this study is to investigate the effects of nano-sized or submicro Fe_2O_3/Fe_3O_4 on the bioreduction of hexavalent chromium(Cr(VI)) and to evaluate the effects of nano-sized Fe_2O_3/Fe_3O_4 on the microbial communities from the anaerobic flooding soil.The results indicated that the net decreases upon Cr(VI) concentration from biotic soil samples amended with nano-sized Fe_2O_3(317.1 ± 2.1 mg/L) and Fe_3O_4(324.0 ± 22.2 mg/L) within21 days,which were approximately 2-fold of Cr(VI) concentration released from blank control assays(117.1 ± 5.6 mg/L).Furthermore,the results of denaturing gradient gel electrophoresis(DGGE) and high-throughput sequencing indicated a greater variety of microbes within the microbial community in amendments with nano-sized Fe_2O_3/Fe_3O_4 than the control assays.Especially,Proteobacteria occupied a predominant status on the phylum level within the indigenous microbial communities from chromium-contaminated soils.Besides,some partial decrease of soluble Cr(VI) in abiotic nano-sized Fe_2O_3/Fe_3O_4 amendments was responsible for the adsorption of nano-sized Fe_2O_3/Fe_3O_4 to soluble Cr(VI).Hence,the presence of nano-sized Fe_2O_3/Fe_3O_4 could largely facilitate the mobilization and biotransformation of Cr(VI) from flooding soils by adsorption and bio-mediated processes. | Yaxian Zhang Hua Li Libo Gong Guowen Dong Liang Shen Yuanpeng Wang Qingbiao Li | 2017 | Journal of Environmental Sciences2017，29，7： | 4 |
| 6 | Cloning of aminopeptidase N promoter and its activity in hematopoietic cell and different tumor cell lines显示文摘Aminopeptidase N (APN) promoter region was cloned and sequenced from peripheral blood mononuclear cells. The recombinant reporter construct containing the promoter and luciferase gene, designated pXPl-APNLuc, was introduced into myeloblastic cell line, T lymphocyte cell line and various tumor cell lines. Luciferase assay showed that APN upstream promoter is myeloid-specific for high expression in myeloblastic cell line and much lower expression in T lymphocyte cell line. The promoter activity was relatively high in lung adenoma cell line compared with other tumor cell lines including hepatoma cell line, tong cancer cell line and esophageal cancer cell line in which the promoter activity significantly diminished or was almost undetectable. The characteristics of APN promoter may provide a new strategy for specific myeloprotection while tumor patients are being treated with chemotherapy and/or radiotherapy. | LIANG Libo MA Yewei ZHAO Qingzheng YANG Jun LIU Yuying WANG Zheng | 2001 | Chinese Science Bulletin2001，46，19： | 4 |
| 7 | Simultaneous evaluation of two branches of a multifunctional integrated optic chip with an ultra-simple dual-channel configuration显示文摘We propose an ultra-simple dual-channel configuration for simultaneously evaluating two branches of a multifunctional integrated optic chip(MFIOC). In the configuration, the MFIOC is employed as a beam splitter to construct the demodulation interferometer together with a 2 × 2 fiber coupler. Interference happens between polarization modes traveling through different channels of the MFIOC. The cross-couplings of each channel are respectively characterized by the interference peaks which distribute on opposite sides of the central interference peak. Temperature responses of the MFIOC are experimentally measured from-40°C to 80°C. Results show that the proposed configuration can achieve simultaneous dual-channel transient measurements with resolution of-90 d B and dynamic range of 90 d B. In addition, the two channels of the configuration have consistent measuring performance, and the two branches of the MFIOC have different responses to temperature variation. | Yonggui Yuan Chuang Li Jun Yang Ai Zhou Shuai Liang Zhangjun Yu Bing Wu Feng Peng Yu Zhang Zhihai Liu Libo Yuan | 2015 | Photonics Research2015，3，4： | 2 |
| 8 | Tim‐3/galectin‐9 signaling pathway mediates T‐cell dysfunction and predicts poor prognosis in patients with hepatitis B virus‐associated hepatocellular carcinoma显示文摘 | Hang Li Ke Wu Kaixiong Tao Libo Chen Qichang Zheng Xiaoming Lu Jun Liu Liang Shi Chuanqiao Liu Guobin Wang Weiping Zou | 2012 | Hepatology2012，，： | 2 |
| 9 | Recent advances in the synthesis of nanoscale hierarchically porous metal–organic frameworks显示文摘Nanoscale hierarchically porous metal–organic frameworks(NHP-MOFs)have received unprecedented attention in many fields owing to their integration of the strengths of nanoscale size(<1μm)and hierarchical porous structure(micro-,meso-and/or macro-pores)of MOFs.This review focuses on recent advances in the main synthetic strategies for NHP-MOFs based on different metal ions(e.g.,Cu,Fe,Co,Zn,Al,Zr,and Cr),including the template method,composite technology,post-synthetic modification,in situ growth and the grind method.In addition,the mechanisms of synthesis,regulation techniques and the advantages and disadvantages of various methods are discussed.Finally,the challenges and prospects of the commercialisation of promising NHP-MOFs are also presented.The purpose of this review is to provide a road map for future design and development of NHP-MOFs for practical application. | Chongxiong Duan Kuan Liang Zena Zhang Jingjing Li Ting Chen Daofei Lv Libo Li Le Kang Kai Wang Han Hu Hongxia Xi | 2022 | Nano Materials Science2022，4，4： | 2 |
| 10 | 急腹症罕见诱因:巨大胃间质瘤自发破裂(英文)显示文摘A case of spontaneous rupture of giant gastrointestinal stromal tumor(GIST) of stomach causing acute abdomen is described below. A male patient with abdominal mass presented with symptoms and signs of acute abdomen after admitting for 1 day. After preoperative management and evaluation, an exploratory laparotomy was performed, where rupture of a huge tumor in the stomach was found. A subtotal gastrectomy including the mass was performed and the final immunohistochemical examination verified that the neoplasm was a high risk GIST. The postoperative period was uneventful and the patient received treatment with imatinib mesylate, and regular follow-up without recurrence. | Libo Feng Dong Xia Qing Liu Yi Liu Liang Xu | 2014 | The Chinese-German Journal of Clinical Oncology2014，13，4： | 1 |
| 11 | Fiber optic 2-D sensor for measuring the strain inside the concrete specimen显示文摘 | Libo Yuan Qingbin Li Yijun Liang | 2001 | Sensors and Actuators A2001，94，： | 1 |
| 12 | CDK6 inhibits lymphoid cell infiltration and represents a prognostic marker in HPV+ squamous cell carcinoma of head and neck显示文摘Objective:We investigated the correlations between cyclin-dependent kinase 4/6(CDK4/6)levels and human papillomavirus(HPV)infection state in head and neck squamous cell cancer(HNSCC).The aim was to explore the potential value of CDK4/6 inhibitors in the treatment of HNSCC.Methods:Multiomic sequencing data for HNSCC were obtained from The Cancer Genome Atlas(TCGA),and the mRNA levels and copy number variations(CNVs)of CDK4 and CDK6 were strictly analyzed.Overall survival(OS)curves were produced using the Kaplan-Meier method,and survival differences between groups were assessed by the log-rank test.Next,gene set enrichment analysis(GSEA)was applied to interrogate CDK4/6-associated molecular pathways in HPV-positive(HPV+)and HPV-negative(HPV-)HNSCC.Last,lymphoid cell infiltrates in each type of HNSCC were explored,and the correlations between CDK4/6 expression and lymphoid infiltrates were explored by Tumor Immune Estimation Resource(TIMER)analysis.Results:Overexpression of either CDK6 or CDK4 was not a relevant factor for OS in HPV-HNSCC(CDK6:top 40%vs.bottom 40%,P=0.885;CDK4:top 40%vs.bottom 40%,P=0.267).In HPV+HNSCC,CDK6 but not CDK4 was a relevant factor for OS(CDK6:top 40%vs.bottom 40%,P=0.002;CDK4:top 40%vs.bottom 40%,P=0.452).GSEA found that overexpressed CDK6 in HPV+HNSCC inhibited pathways involved in the tumor immune response,suggesting its roles in antitumor immunity.TIMER analysis results revealed that CDK6 but not CDK4 accumulation was negatively correlated with the number of tumor-infiltrating lymphocytes specific for HPV+HNSCC,which led to tumor response suppression.Conclusions:CDK6,but not CDK4,is a poor prognostic marker specific in HPV+HNSCC patients.Overexpressed CDK6 might stimulate tumor progression by suppressing lymphocytes infiltration independent of its kinase activity.Only abrogating its kinase activity using current CDK4/6 inhibitors was not enough to block its tumor promotion function. | Chuanhao Tang Li Lin Wei Zhou Xiang Liu Yali Fu Lingling Zhang Li Li Xiangyi Wang Libo Zhao Jun Liang Libo Zhao Jun Liang | 2019 | Chinese Journal of Cancer Research2019，31，6： | 1 |
| 13 | Effect of hydration pretreatment on tunnel etching behaviour of aluminium foil显示文摘 | LIANG Libo HE Yedong SONG Hongzhou YANG Xiaofei CAI Xiaoyu XIONG Chuanyong LI Yingri | 2013 | Corrosion Science2013，70，： | 1 |
| 14 | Time- and temperature-dependence of compressive and tensile behaviors of polypropylene fiber-reinforced cemented paste backfill显示文摘The understanding of compressive and tensile behaviors of polypropylene fiber-reinforced cemented paste backfill(FR-CPB)play crucial roles in the successful implementation of reinforcement technique in underground mine backfilling operations.However,very limited studies have been performed to gain insight into the evolution of compressive and tensile behaviors and associated mechanical properties of FR-CPB under various curing temperatures from early to advanced ages.Thus,this study aims to investigate the time(7,28,and 90 d)-and temperature(20°C,35°C,and 45°C)-dependence of constitutive behavior and mechanical properties of FR-CPB.The obtained results show that pre-and post-failure behaviors of FR-CPB demonstrate strongly curing temperature-dependence from early to advanced ages.Moreover,the pseudo-hardening behavior is sensitive to curing temperature,especially at early ages.Furthermore,the mechanical properties including elastic modulus,material stiffness,strengths,brittleness,cohesion,and internal friction angle of FR-CPB show increasing trends with curing temperature as curing time elapses.Additionally,a predictive model is developed to capture the strong correlation between compressive and tensile strength of FR-CPB.The findings of this study will contribute to the successful implementation of FR-CPB technology. | Iarley Loan Sampaio LIBOS Liang CUI | 2021 | Frontiers of Structural and Civil Engineering2021，15，4： | 1 |
| 15 | Controlling Ebola: what we can learn from China’s 1911 battle against the pneumonic plague in Manchuria显示文摘 | He Liu Mingli Jiao Siqi Zhao Kai Xing Ye Li Ning Ning Libo Liang Qunhong Wu Yanhua Hao | 2015 | International Journal of Infectious Diseases2015，，： | 1 |
| 16 | Immunosenescence:molecular mechanisms and diseases显示文摘Infection susceptibility,poor vaccination efficacy,age-related disease onset,and neoplasms are linked to innate and adaptive immune dysfunction that accompanies aging(known as immunosenescence).During aging,organisms tend to develop a characteristic inflammatory state that expresses high levels of pro-inflammatory markers,termed inflammaging.This chronic inflammation is a typical phenomenon linked to immunosenescence and it is considered the major risk factor for age-related diseases.Thymic involution,naïve/memory cell ratio imbalance,dysregulated metabolism,and epigenetic alterations are striking features of immunosenescence.Disturbed T-cell pools and chronic antigen stimulation mediate premature senescence of immune cells,and senescent immune cells develop a proinflammatory senescence-associated secretory phenotype that exacerbates inflammaging.Although the underlying molecular mechanisms remain to be addressed,it is well documented that senescent T cells and inflammaging might be major driving forces in immunosenescence.Potential counteractive measures will be discussed,including intervention of cellular senescence and metabolic-epigenetic axes to mitigate immunosenescence.In recent years,immunosenescence has attracted increasing attention for its role in tumor development.As a result of the limited participation of elderly patients,the impact of immunosenescence on cancer immunotherapy is unclear.Despite some surprising results from clinical trials and drugs,it is necessary to investigate the role of immunosenescence in cancer and other age-related diseases. | Zaoqu Liu Qimeng Liang Yuqing Ren Chunguang Guo Xiaoyong Ge Libo Wang Quan Cheng Peng Luo Yi Zhang Xinwei Han | 2023 | Signal Transduction and Targeted Therapy2023，8，6： | 1 |
| 17 | Fiber optic 2-D sensor for measuring the strain inside the concrete specimen 显示文摘 | YUAN Libo LI Qingbin LIANG Yongjun | 2001 | Sensors and Actuators A-Physical2001，94，12： | 1 |
| 18 | Incidental prostate cancer in Asian men: High prevalence of incidental prostatic adenocarcinoma in Chinese patients undergoing radical cystoprostatectomy for treatment of bladder cancer and selection of candidates for prostate‐sparing cystectomy显示文摘 | Xinyu Yang M. Francesca Monn Libo Liu Yuli Liu Jinwei Su Tianjing Lyu Yanqing Gong Lisha Wang Darrell D. Davidson Liang Cheng | 2015 | Prostate2015，，： | 1 |
| 19 | Mutation-associated transcripts reconstruct the prognostic features of oral tongue squamous cell carcinoma显示文摘Tongue squamous cell carcinoma is highly malignant and has a poor prognosis.In this study,we aimed to combine whole-genome sequencing,whole-genome methylation,and whole-transcriptome analyses to understand the molecular mechanisms of tongue squamous cell carcinoma better.Oral tongue squamous cell carcinoma and adjacent normal tissues from five patients with tongue squamous cell carcinoma were included as five paired samples.After multi-omics sequencing,differentially methylated intervals,methylated loop sites,methylated promoters,and transcripts were screened for variation in all paired samples.Correlations were analyzed to determine biological processes in tongue squamous cell carcinoma.We found five mutated methylation promoters that were significantly associated with mRNA and lncRNA expression levels.Functional annotation of these transcripts revealed their involvement in triggering the mitogen-activated protein kinase cascade,which is associated with cancer progression and the development of drug resistance during treatment.The prognostic signature models constructed based on WDR81 and HNRNPH1 and combined clinical phenotype-gene prognostic signature models showed high predictive efficacy and can be applied to predict patient prognostic risk in clinical settings.We identified biological processes in tongue squamous cell carcinoma that are initiated by mutations in the methylation promoter and are associated with the expression levels of specific mRNAs and lncRNAs.Collectively,changes in transcript levels affect the prognosis of tongue squamous cell carcinoma patients. | Libo Liang Yi Li Binwu Ying Xinyan Huang Shenling Liao Jiajin Yang Ga Liao | 2023 | International Journal of Oral Science2023，15，1： | 1 |
| 20 | Injectable nanofiber-reinforced bone cement with controlled biodegradability for minimally-invasive bone regeneration显示文摘Injectable materials show their special merits in regeneration of damaged/degenerated bones in minimally-invasive approach.Injectable calcium phosphate bone cement(CPC)has attracted broad attention for its bioactivity,as compared to non-degradable polymethyl methacrylate cement.However,its brittleness,poor anti-washout property and uncontrollable biodegradability are the main challenges to limit its further clinical application mainly because of its stone-like dense structure and fragile inorganic-salt weakness.Herein,we developed a kind of injectable CPC bone cement with porous structure and improved robustness by incorporating poly(lactide-co-glycolic acid)(PLGA)nanofiber into CPC,with carboxymethyl cellulose(CMC)to offer good injectability as well as anti-wash-out capacity.Furthermore,the introduction of PLGA and CMC also enabled a formation of initial porous structure in the cements,where PLGA nanofiber endowed the cement with a dynamically controllable biodegradability which provided room for cell movement and bone ingrowth.Inter-estingly,the reinforced biodegradable cement afforded a sustainable provision of Ca^(2+)bioactive components,together with its porous structure,to improve synergistically new bone formation and osteo-integration in vivo by using a rat model of femur condyle defect.Further study on regenerative mechanisms indicated that the good minimally-invasive bone regeneration may come from the synergistic enhanced osteogenic effect of calcium ion enrichment and the improved revascularization capacity contributed from the porosity as well as the lactic acid released from PLGA nanofiber.These results indicate the injectable bone cement with high strength,anti-washout property and controllable biodegradability is a promising candidate for bone regeneration in a minimally-invasive approach. | Peihao Cai Shunyi Lu Jieqin Yu Lan Xiao Jiayi Wang Haifeng Liang Lei Huang Guanjie Han Mengxuan Bian Shihao Zhang Jian Zhang Changsheng Liu Libo Jiang Yulin Li | 2023 | Bioactive Materials2023，，3： | 1 |
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