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1Experimental study on hydration damage mechanism of shale from the Longmaxi Formation in southern Sichuan Basin, China显示文摘As a serious problem in drilling operation,wellbore instability restricts efficient development of shale gas.The interaction between the drilling fluid and shale with hydration swelling property would have impact on the generation and propagation mechanism of cracks in shale formation,leading to wellbore instability.In order to investigate the influence of the hydration swelling on the crack propagation,mineral components and physicochemical properties of shale from the Lower Silurian Longmaxi Formation(LF)were investigated by using the XRD analysis,cation exchange capabilities(CEC)analysis,and SEM observation,and we researched the hydration mechanism of LF shale.Results show that quartz and clay mineral are dominated in mineral composition,and illite content averaged 67%in clay mineral.Meanwhile,CEC of the LF shale are 94.4 mmol/kg.The process of water intruding inside shale along microcracks was able to be observed through high power microscope,meanwhile,the hydration swelling stress would concentrate at the crack tip.The microcracks would propagate,bifurcate and connect with each other,with increase of water immersing time,and it would ultimately develop into macro-fracture.Moreover,the macrocracks extend and coalesce along the bedding,resulting in the rock failure into blocks.Hydration swelling is one of the major causes that lead to wellbore instability of the LF shale,and therefore improving sealing capacity and inhibition of drilling fluid system is an effective measure to stabilize a borehole.Xiangjun Liu Wei Zeng Lixi Liang Jian Xiong 2016Petroleum2016,2,1:14
2对于提高奥氏体不锈钢离子氮化表面腐蚀性能的措施显示文摘离子氮化已经非常成功地应用在奥氏体不锈钢的表面强化方面,以提高其耐磨性与摩擦学性能。然而由于氮化铬在氮化层中沉淀析出,在许多受环境严重腐蚀的应用场合,使传统的离子氮化耐腐蚀性能恶化,这是无法接受的。最近几年,作者企图提高奥氏体不锈钢离子氮化的耐腐蚀性能,以获得既耐磨又耐腐蚀的表面。为此,进行了两方面的探索即传统的高温氮化后紧接着表面钝化处理,以及低温氮化处理。前者产生一种较厚且耐磨的亚表面氮化层和耐腐蚀的表面钝化膜。而低温氮化处理可产生一层薄且硬的无沉淀析出的非常耐腐蚀的氮化物层。本文讲述了针对奥氏体不锈钢进行各种氮化处理的最新试验结果,并讨论了其显微组织、动力学、耐磨性和耐腐蚀性能。T.BELL LiXY SunY 刘家浚 张秋英 1998中国表面工程1998,11,4:13
3Whole-Genome Resequencing of a Worldwide Collection of Rapeseed Accessions Reveals the Genetic Basis of Ecotype Divergence显示文摘Rapeseed (Brassica napus),an important oilseed crop,has adapted to diverse climate zones and latitudes by forming three main ecotype groups,namely winter,semiwinter,and spring types. However,genetic variations underlying the divergence of these ecotypes are largely unknown. Here,we report the global pattern of genetic polymorphisms in rapeseed determined by resequencing a worldwide collection of 991 germplasm accessions.A total of 5.56 and 5.53 million singlenucleotide polymorphisms (SNPs)as Well as 1.86 and 1.92 million InDels were identified by mapping reads to the reference genomes of 'Darmor-bzh'and 'Tapidor,'respectively.We generated a map of allelic drift paths that shows splits and mixtures of the main populations,and revealed an asymmetric evolution of the two subgenomes of B.napus by calculating the genetic diversity and linkage disequilibrium parameters.Selective-sweep analysis revealed genetic changes in genes orthologous to those regulating various aspects of plant development and response to stresses.A genome-wide association study identified SNPs in the promoter regions of FLOWERING LOCUS T and FLOWERING LOCUS C orthologs that corresponded to the different rapeseed ecotype groups. Our study provides important insights into the genomic footprints of rapeseed evolution and flowering-time divergence among three ecotype groups,and will facilitate screening of molecular markers for accelerating rapeseed breeding.Dezhi Wu Zhe Liang Tao Yan Ying Xu Lijie Xuan Juan Tang Gang Zhou Ulrike Lohwasser Shuijin Hua Haoyi Wang Xiaoyang Chen Qian Wang Le Zhu Antony Maodzeka Nazim Hussain Zhilan Li Xuming Li Imran Haider Shamsi Ghulam Jilani Linde Wu Hongkun Zheng Guoping Zhang Boulos Chalhoub Lisha Shen Hao Yu Lixi Jiang 2019Molecular Plant2019,12,1:10
4Bioflocculant Produced by Klebsiella sp. MYC and Its Appli-cation in the Treatment of Oil-field Produced Water显示文摘Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension and oil-field produced water. The bacterial strain was identified as Klebsiella sp. MYC according to its morphological and biochemical characteristics and 16S rDNA sequence. The optimal medium for bioflocculant production by this bacterial strain was composed of cane sugar 20 g L -1, KH2PO4 2 g L -1, K2HPO4 5 g L -1, (NH4)2SO4 0.2 g [KG-*9]L -1, urea 0.5 g L -1 and yeast extract 0.5 g L -1, the initial pH being 5.5. When the suspension of kaolin clay was treated with 0.5% of Klebsiella sp. MYC culture broth, the flocculating rate reached more than 90.0% in the presence of 500 mg L -1 CaCl2, while the flocculating rate for oil-field produced water was near 80[KG-*3].0% in a pH range of 7.0-9.0 with the separation of oil and suspended particles from the oil-field produced water under similar conditions. The environment-friendly nature of the bioflocculant and high flocculating rate of the strain make the bioflocculant produced by Klebsiella sp. MYC an attractive bioflocculant in oil-field produced water treatment.YUE Lixi MA Chunling CHI Zhenming 2006Journal of Ocean University of China2006,5,4:7
5Electromagnetic and microwave absorbing properties of W-type barium ferrite doped with Gd^(3+)显示文摘W-type barium ferrites doped with Gd3+,Ba1-xGdx(Zn0.3Co0.7)2Fe16O27(x = 0,0.05,0.10,0.15,0.20),were prepared by a sol-gel method.The effects of Gd3+ substitution on their microstructure,electromagnetic properties and microwave absorptive behavior were analyzed.The XRD patterns showed the single phase of W-type barium ferrite when x ≤ 0.15.Microwave electromagnetic properties of samples were studied at the frequency range from 2 GHz to 18 GHz using a network analyzer(Agilent 8722ET).The complex permittivity ε(ε',ε'') increased gradually when x ≤ 0.10,but it decreased as x = 0.15.The real permeability(μ') decreased with the increase of Gd3+ content,while the imaginary permeability(μ'') increased when x ≤ 0.10.All these reasons were discussed using the electromagnetic theory.Furthermore,the ferrite-epoxy compound coating materials with 80 wt.% of Ba0.9Gd0.1(Zn0.3Co0.7)2Fe16O27 were prepared to measure the microwave absorbing properties.The maximum of reflection loss(RL) reached about-27 dB and RL was below-10 dB in the frequency range of 8-18 GHz when the thickness was 1.92 mm.HUANG Xiaogu CHEN Jiao WANG Lixi ZHANG Qitu 2011Rare Metals2011,30,1:7
6State-of-the-Art Review of High-Throughput Statistical Spatial-Mapping Characterization Technology and Its Applications显示文摘Macroscopic materials are heterogeneous,multi-elementary,and complex.No material is homogeneous or isotropic at a certain small scale.Parts of the material that differ from one another can be termed'natural chips.'At different spots on the material,the composition,structure,and properties vary slightly,and the combination of these slight differences establishes the overall material performance.This article presents a state-of-the-art review of research and applications of high-throughput statistical spatialmapping characterization technology based on the intrinsic heterogeneity within materials.Highthroughput statistical spatial-mapping uses a series of rapid characterization techniques for analysis from the macroscopic to the microscopic scale.Datasets of composition,structure,and properties at each location are obtained rapidly for practical sample sizes.Accurate positional coordinate information and references to a point-to-point correspondence are used to set up a database that contains spatialmapping lattices.Based on material research and development design requirements,dataset spatialmapping within required target intervals is selected from the database.Statistical analysis can be used to select a suitable design that better meets the targeted requirements.After repeated verification,genetic units that reflect the material properties are determined.By optimizing process parameters,the assembly of these genetic unit(s)is verified at the mesoscale,and quantitative correlations are established between the microscale,mesoscale,macroscale,practical sample,across-the-scale span composition,structure,and properties.The high-throughput statistical spatial-mapping characterization technology has been applied to numerous material systems,such as steels,superalloys,galvanization,and ferrosilicon alloys.This approach has guided the composition and the process optimization of various materials.Haizhou Wang Lei Zhao Yunhai Jia Dongling Li Lixi Yang Yuhua Lu Guang Feng Weihao Wan 2020Engineering2020,6,6:7
7The influence of water-based drilling fluid on mechanical property of shale and the wellbore stability显示文摘Because of high cost and pollution of oil-based drilling fluid,the water-based drilling fluid is increasingly used now.However,bedding planes and micro-cracks are rich in shale formation.When water-based drilling fluid contacts formation rock,it causes the propagation of crack and invasion of drilling fluid,which decrease shale strength and cause wellbore instability.In this paper,we analyzed influence of water-based drilling fluid on shale strength and failure mode by mechanics experiment.Based on those experimental results,considering the effect of bedding plane and drilling time,we established modeling of wellbore stability for shale formation.The result from this model indicates that in certain azimuth of horizontal well,collapsing pressure increases dramatically due to shale failure along with bedding plane.In drilling operation,those azimuths are supposed to be avoided.This model is applicable for predication of collapsing pressure in shale formation and offers reference for choosing suitable mud weight.Shu He Lixi Liang Yinjin Zeng Yi Ding Yongxue Lin Xiangjun Liu 2016Petroleum2016,2,1:6
8The association between early-onset cardiac events caused by neoadjuvant or adjuvant chemotherapy in triple-negative breast cancer patients and some novel autophagy-related polymorphisms in their genomic DNA: a real-world study显示文摘Background:An increasing number of cancer patients die of cardiovascular diseases.The cardiotoxicity of chemo-therapy is particularly important in triple-negative breast cancer(TNBC)with limited therapeutic options.Cardiac autophagy is an important mechanism of cardiotoxicity.This research was aimed to investigate the cardiotoxicity of chemotherapy in TNBC,screen the susceptible population,and determine the relationship between cardiotoxicity and autophagy-related polymorphisms.Methods:From a total of 2450 stage I-III TNBC patients,147 met the inclusion criteria and finally recruited.Electro-cardiography(ECG)was performed before most chemotherapy cycles,and echocardiography(UCG)was performed according to clinical needs.All ECG and UCG records were re-interpreted by cardiologists at the National Center for Cardiovascular Disease,Fuwai Hospital.According to the National Center for Biotechnology Information and the Catalog of Somatic Mutations in Cancer database,we selected 25 single nucleotide polymorphisms(SNPs)related to autophagy and genotyped the 147 TNBC patients.Paired-sample T tests,Chi squared tests,and logistic regression models were employed for the analysis.Results:Only 46(31.3%)patients had normal ECG records after every chemotherapy cycle.Among the 16 patients who underwent UCG,2(12.5%)had a reversible decrease of left ventricular ejection fraction.The use of anthracyclines and excessive alcohol consumption were risk factors of ECG abnormalities.With the continuation of chemotherapy,heart rate gradually increased.Anthracyclines were associated with QRS duration abnormalities(P=0.043).After genotyping for 25 autophagy-related SNPs,we found that the G allele of autophagy-related 13(ATG13)rs10838611 was significantly associated with ECG abnormalities(odds ratio=2.258,95%confidence interval=1.318-3.869;P=0.003).Conclusion:ECG abnormalities caused by chemotherapy are common in the real world.Autophagy-related SNPs are associated with chemotherapy-induced cardiotoxicity,thereby providing new evidence for autophagy as a cause of chemotherapy-induced cardiac damage.Binliang Liu Tao An Meiying Li Zongbi Yi Chunxiao Li Xiaoying Sun Xiuwen Guan Lixi Li Yanfeng Wang Yuhui Zhang Binghe Xu Fei Ma Yixin Zeng 2018Cancer Communications2018,38,1:5
9Differential gene expression in human hepatocellular carcinoma Hep3B cells induced by apoptosis-related gene BNIPL-2显示文摘AIM:Bcl-2/adenovirus E1B 19 ku interacting protein 2-like (BNIPL-2) is a novel protein recently identified in our laboratory.BNIPL-2 is homologous to human BNIP-2,a potentially proapoptotic protein,and can interact with Bcl-2 and Cdc42GAP and promote apoptosis in BEL-7402 cells.Here we report the gene-expression profile regulated by BNIPL-2 in human hepatocarcinoma Hep3B cells and the analysis of its potential roles in cell apoptosis.METHODS:BNIPL-2 was overexpressed in Hip3B cells using tetracycline indecible or Tet-on system.Screened by Western blot,the cells with low background and high induction fold if BNIPL-2 were obtained.We performed Altlas human cDNA expression array hybridization on these cells and analyzed the data with Quantarray coftwate to identify BNIPL-2-regulated genes and their expression profile.RT-PCR was used to confirm the altered expression level of part of genes identified by the Atlas arrar hybridization.RESULTS:Fifteen of 588 genes spotted on the Atlas membrane showed altered expression levels in BNIPL-2-transfected Hep3B-Tet-on cells,in which 8 genes involved in cell apoptosis or growth inhibition were uregulated and 7 genes involved in cellular proliferation were down-regulated following overexpression of BNIPL-2.CONCLUSION:cDNA arrar is a powerful tool to explore gene expression profiles under inducible conditions.The data obtained usind the cDNA expression microarray techonlogy indicates that BNIPL-2 may play its roles in apoptosis through regulating the expression of gnees associated with cell apoptosis,growth inhibition and cell proliferation .LiXie Wen-XinQin Xiang-HuoHe Hui-QunShu Gen-FuYao Da-FangWan Jian-RenGu 2004World Journal of Gastroenterology2004,10,9:4
10Microstructure and microwave electromagnetic properties of Dy^(3+)-doped W-type hexaferrites显示文摘Ba 1 x Dy x Co 2 Fe 16 O 27 (x = 0.00, 0.05, 0.10, 0.15, and 0.20) was prepared by the solid-state method. The phase structure was studied using powder X-ray diffraction (XRD), the electromagnetic properties were measured, and the reflection loss of Dy 3+ -doped ferrite material was calculated using electromagnetic parameters by the transmission line theory. All XRD patterns showed the single phase of the magnetoplumbite barium ferrite without other intermediate phase when x ≤ 0.15. The values of ε′ and ε″ increased slightly with Dy 3+ ions doping. The values of μ″ and μ′ were improved with Dy 3+ doping, exhibiting excellent microwave magnetic performance. The reasons have also been discussed using the electromagnetic theory. Dy substitution could increase microwave-absorbing performance and broaden frequency band (reflection loss (RL) < -10 dB), and the absorbing peak shifted to high-frequency position. When x = 0.2, ferrite layer exhibited the most excellent microwave-absorbing performance at a thin matching thickness of 1.5 mm. The peak value of RL was around -15 dB, and the frequency band (RL < -10 dB) was about 7 GHz (from 8 to 15 GHz).WANG Lixi HUANG Xiaogu ZHANG Jing WANG Hongzhou ZHANG Qitu XU Naicen 2011Rare Metals2011,30,5:4
11Two single nucleotide polymorphisms in the GDF5 gene are associated with development dysplasia of the hip in Chinese female population显示文摘Development dysplasia of the hip(DDH)is one of the most common hip defects in newborns.The main phenotypes of the disease are partial or whole femoral head extrusion from the acetabulum[1].Because the frequency of DDH in monozygotic twins was higher than that in dizygotic twins,and the affected individuals were often in the same family[2],we are focusing on the genetic factor in the present study.ZHAO LiXi PAN Hong WANG Jing CHENG Zhi CHENG LongFei WANG BinBin MA Xu 2013Science China(Life Sciences)2013,56,11:3
12AstudyofthesystemandnewtaxaofgenusGlycyrrhizaL[J]显示文摘LIXY(李学禹) 植物研究0,,:2
13Microwave Absorbing Properties of Magnesium-substituted MnZn Ferrites Prepared by Citrate-EDTA Complexing Method显示文摘Magnesium-substituted MnZn ferrites with composition of Mn0.4Zn0.6MgxFe(2-x)O4(x=0.00,0.05) were prepared by citrate-EDTA(ethylenediamine tetraacetic acid) complexing method.The crystal structure,surface morphology and electromagnetic properties of the calcined samples were characterized by X-ray diffraction(XRD) analysis,scanning electron microscopy(SEM) and network analyzer(Agilent 8722ET).All the XRD patterns showed the single phase of the spinel-type MnZn ferrite without other intermediate phase.The lattice parameter and average particle size increased with the substitution of magnesium.The microwave electromagnetic properties of the samples have been studied at the frequency range from 2 to 18 GHz.It was shown that the values of ε′ and ε″ decreased slightly with the substitution of magnesium.The values of μ′ and μ″ were improved significantly,and the peak of μ″ shifted to low frequency with Mg2+ ions substituting.Furthermore,the reasons have also been discussed by using electromagnetic theory.The reflection loss(RL) ascended after magnesium was substituted.Magnesium-substituted ferrite had excellent microwave absorption properties.The frequency(with respect to-10 dB) started from 11.3 GHz,and the bandwidth reached about 3.5 GHz.The peak value of RL was about-19 dB at a matching thickness of 3.1 mm.Jie Song Lixi Wang Naicen Xu Qitu Zhang 2010Journal of Materials Science & Technology2010,26,9:2
14Research on fabric characteristics and borehole instability mechanisms of fractured igneous rocks显示文摘There are favorable exploration prospects in igneous rock reservoirs.However,problems of borehole instability occur frequently during drilling igneous formations,which is a serious impediment to oil and gas exploration and production.The lack of systematic understanding of the inherent instability mechanisms is an important problem.A series of experiments were conducted on several igneous rock samples taken from the sloughing formations in the Tuha area in an attempt to reveal the inherent mechanisms of wellbore instability when drilling in fractured igneous rocks.Research methods involved slurry chemistry,analysis of micro-geological features(Micro-CT imaging,SEM),and rock mechanics testing.The experimental results indicated that clay minerals were widely distributed in the intergranular space of the diagenetic minerals,crystal defects,and microcracks.Drilling fluid filtrate would invade the rock along the microcracks.The invasion amount gradually increased over time,which constantly intensified the hydration and swelling of clay minerals,leading to changes in the microscopic structure of igneous rocks.Primary and secondary microcracks can propagate and merge into single cracks and thus reducing rock cohesion and the binding force along cleavage planes.Based on this result the authors propose that a key towards solving wellbore instability in igneous formations is that specific micro-geological characteristics of the igneous rocks should be taken into consideration in the design of antisloughing drilling muds.Liu Xiangjun Zhu Honglin Liang Lixi 2013Petroleum Science2013,10,2:2
15The genome and gene editing system of sea barleygrass provide a novel platform for cereal domestication and stress tolerance studies显示文摘The tribe Triticeae provides important staple cereal crops and contains elite wild species with wide geneticdiversity and high tolerance to abiotic stresses. Sea barleygrass (Hordeum marinum Huds.), a wildTriticeae species, thrives in saline marshlands and is well known for its high tolerance to salinity and waterlogging. Here, a 3.82-Gb high-quality reference genome of sea barleygrass is assembled de novo, with 3.69Gb (96.8%) of its sequences anchored onto seven chromosomes. In total, 41 045 high-confidence (HC)genes are annotated by homology, de novo prediction, and transcriptome analysis. Phylogenetics, nonsynonymous/synonymous mutation ratios (Ka/Ks), and transcriptomic and functional analyses provide genetic evidence for the divergence in morphology and salt tolerance among sea barleygrass, barley, andwheat. The large variation in post-domestication genes (e.g. IPA1 and MOC1) may cause interspecies differences in plant morphology. The extremely high salt tolerance of sea barleygrass is mainly attributed tolow Na+ uptake and root-to-shoot translocation, which are mainly controlled by SOS1, HKT, and NHX transporters. Agrobacterium-mediated transformation and CRISPR/Cas9-mediated gene editing systems weredeveloped for sea barleygrass to promote its utilization for exploration and functional studies of hubgenes and for the genetic improvement of cereal crops.Liuhui Kuang Qiufang Shen Liyang Chen Lingzhen Ye Tao Yan Zhong-Hua Chen Robbie Waugh Qi Li Lu Huang Shengguan Cai Liangbo Fu Pengwei Xing Kai Wang Jiari Shao Feibo Wu Lixi Jiang Dezhi Wu Guoping Zhang 2022Plant Communications2022,3,5:2
16Chiral assembly of achiral pseudoisocyanine with D-and L-phenylalanine显示文摘Supramolecular chirality and molecular self-assembly are important and interesting phenomena in living and non-living systems.In this work,supramolecular chirality of achiral pseudoisocyanine(PIC) J-aggregates was successfully induced by D-,L-phenylalanine(Phe) and other amino acids in NaCl solution.The chiral J-aggregates showed a characteristic,induced circular dichroism(ICD) in the visible region of J-band chromophore which depends on the absolute configuration,concentration and side groups of α-amino acids,as well as temperature.The atomic force microscopy images indicated that the J-aggregates exist in large bundles of entangled nanofibers,and the observed ICD might result from the macroscopic helical arrangement of the assemblies.ZENG LiXi HE YuJian DAI ZhiFeng WANG Jian WANG CaiQi YANG YongGang 2009Science China Chemistry2009,52,8:2
17Antibody-drug conjugates in HER2-positive breast cancer显示文摘Antibody-drug conjugates(ADCs)combine the high specificity of monoclonal antibodies with the high anti-tumor activity of small molecular cytotoxic payloads.The anti-tumor activity of ADCs is mainly achieved by the direct blocking of the receptor by monoclonal antibodies,direct action and bystander effect of cytotoxic drugs,and antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity.ADCs have been used in adjuvant therapy and rescue treatment of human epidermal receptor 2(HER2)-positive breast cancer,greatly improving the prognosis of breast cancer patients.Several ongoing clinical trials of ADC for breast cancer and other solid tumors proved the potential of ADCs will provide more promising treatment options for patients with malignant tumors.This review introduces the mechanism and latest clinical progress of ADC drugs approved for HER2-positive breast cancer to guide clinical practice and conduct research.Lixi Li Di Zhang Binliang Liu Dan Lv Jingtong Zhai Xiuwen Guan Zongbi Yi Fei Ma 2022Chinese Medical Journal2022,,3:2
18Efficient separation between trivalent americium and lanthanides enabled by a phenanthroline-based polymeric organic framework显示文摘Separation of the minor actinides(Am and Cm)from lanthanides in high-level liquid wastes(HLLW)is one of the most challenging chemical separation tasks known owing to their chemical similarities and is highly significant in nuclear fuel reprocessing plants because it could practically lead to sustainable nuclear energy by closing the nuclear fuel cycle.The solid phase extraction is proposed to be a possible strategy but all reported sorbent materials severely suffer from limited stability and/or efficiency caused by the harsh conditions of high acidity coupled with intense irradiation.Herein,a phenanthroline-based polymeric organic framework(PhenTAPB-POF)was designed and tested for the separation of trivalent americium from lanthanides for the first time.Due to its fully conjugated structure,PhenTAPB-POF exhibits previously unachieved stability under the combined extreme conditions of strong acids and high irradiation field.The americium partitioning experiment indicates that PhenTAPB-POF possesses an ultrahigh adsorption selectivity towards Am(Ⅲ)over lanthanides(e.g.,SFAm(Ⅲ)/Eu(Ⅲ)=3326)in highly acidic simulated HLLW and relatively fast adsorption kinetics in both static and dynamic experiments.Am(Ⅲ)can be almost quantitatively eluted from the PhenTAPB-POF packed-column using a concentrated nitric acid elution.The high stability and superior separation performance endow PhenTAPB-POF with the promising alternative for separating minor actinides over lanthanides from highly acidic HLLW streams.Afshin Khayambashi Long Chen Xue Dong Kai Li Zhipeng Wang Linwei He Suresh Annam Lixi Chen Yaxing Wang Matthew V.Sheridan Chao Xu Shuao Wang 2022Chinese Chemical Letters2022,33,7:1
19Association analysis between HOXD9 genes and the development of developmental dys- plasia of the hip in Chinese female Han population显示文摘Wei Tian Lixi Zhao Jing Wang 2012BMC Musculoskeletal Disorders2012,13,:1
20显示文摘Mu Lei Huang Yun Wang lixi 2007Journal of Rare Earths Suppl2007,25,:1
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