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| 1 | The genome and gene editing system of sea barleygrass provide a novel platform for cereal domestication and stress tolerance studies显示文摘The tribe Triticeae provides important staple cereal crops and contains elite wild species with wide geneticdiversity and high tolerance to abiotic stresses. Sea barleygrass (Hordeum marinum Huds.), a wildTriticeae species, thrives in saline marshlands and is well known for its high tolerance to salinity and waterlogging. Here, a 3.82-Gb high-quality reference genome of sea barleygrass is assembled de novo, with 3.69Gb (96.8%) of its sequences anchored onto seven chromosomes. In total, 41 045 high-confidence (HC)genes are annotated by homology, de novo prediction, and transcriptome analysis. Phylogenetics, nonsynonymous/synonymous mutation ratios (Ka/Ks), and transcriptomic and functional analyses provide genetic evidence for the divergence in morphology and salt tolerance among sea barleygrass, barley, andwheat. The large variation in post-domestication genes (e.g. IPA1 and MOC1) may cause interspecies differences in plant morphology. The extremely high salt tolerance of sea barleygrass is mainly attributed tolow Na+ uptake and root-to-shoot translocation, which are mainly controlled by SOS1, HKT, and NHX transporters. Agrobacterium-mediated transformation and CRISPR/Cas9-mediated gene editing systems weredeveloped for sea barleygrass to promote its utilization for exploration and functional studies of hubgenes and for the genetic improvement of cereal crops. | Liuhui Kuang Qiufang Shen Liyang Chen Lingzhen Ye Tao Yan Zhong-Hua Chen Robbie Waugh Qi Li Lu Huang Shengguan Cai Liangbo Fu Pengwei Xing Kai Wang Jiari Shao Feibo Wu Lixi Jiang Dezhi Wu Guoping Zhang | 2022 | Plant Communications2022，3，5： | 2 |
| 2 | Injectable bio-responsive hydrogel for therapy of inflammation related eyelid diseases显示文摘Eyelid plays a vital role in protecting the eye from injury or infection.Inflammation related eyelid diseases,such as blepharitis,are the most common ocular disorders that affect human’s vision and quality of life.Due to the physiological barriers and anatomical structures of the eye,the bioavailability of topical administrated therapeutics is typically less than 5%.Herein,we developed a bio-responsive hydrogel drug delivery system using a generally recognized as safe compound,triglycerol monostearate(TG-18),for in-situ eyelid injection with sustained therapeutics release.In vitro,drug release and disassembly time of Rosiglitazone loaded hydrogel(Rosi-hydrogel)were estimated in the presence or absence of MMP-9,respectively.Moreover,the disassembly of TG-18 hydrogel was evaluated with 9-month-old and 12-month-old mice in vivo.Owing to the bio-responsive nature of Rosi-hydrogel,the on-demand Rosiglitazone release is achieved in response to local enzymes.These findings are proved by further evaluation in the age-related meibomian gland dysfunction mice model,and the bio-responsive hydrogel is used as an in-situ injection to treat eyelid diseases.Taken together,the in-situ eyelid injection with sustained drug release opens a window for the therapy of inflammation related eyelid diseases. | Liangbo Chen Dan Yan Nianxuan Wu Qinke Yao Hao Sun Yan Pang Yao Fu | 2021 | Bioactive Materials2021，6，10： | 1 |
| 3 | Determination of the Concentration of Chlorella vulgaris Beij by Visible Spectrophotometer显示文摘[Objective]This study aimed to provide technical parameters for large-scale production and industrial development of Chlorella vulgaris Beij.[Method]By using 721 visible spectrophotometer,the transmitted light intensity of different concentrations of C.vulgaris liquid under different wavelengths was measured to normalize the absorbance data,thus determining the optimal wavelength for the growth of C.vulgaris and the concentration of C.vulgaris liquid under the optimal wavelength.The regression equation of absorbance at the optimal wavelength and C.vulgaris concentration was obtained to derive the formula between equivalent concentration C and transmitted light intensity I,thus calculating the equivalent concentration of 0.25-fold C.vulgaris liquid under different transmittance.[Result]The transmitted light intensity of C.vulgaris reached the peak at 670 nm,indicating that 670 nm is the optimal wavelength for measuring C.vulgaris concentration.The regression equation between absorbance and C.vulgaris concentration was y=-1.479 7+0.987x,with a correlation coefficient of 0.991,suggesting a high correlation between these two factors.The formula of equivalent concentration C and transmitted light intensity I was C=-ln(I/I0)/LK,where L indicates the optical path length(cuvette width)and K indicates the average cross-sectional area of C.vulgaris cells.[Conclusion]It is feasible to investigate indirectly the concentration of C.vulgaris with a spectrophotometer,which can be used to determine the optimal dehydration time of C.vulgaris. | Zhixu ZHAO Jingyang PAN Liangbo WU Wei ZHANG Junsheng XU Yugui CHEN Zhide FU | 2015 | Agricultural Biotechnology2015，4，2： | 0 |
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