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| 1 | Whole-Genome Resequencing of a Worldwide Collection of Rapeseed Accessions Reveals the Genetic Basis of Ecotype Divergence显示文摘Rapeseed (Brassica napus),an important oilseed crop,has adapted to diverse climate zones and latitudes by forming three main ecotype groups,namely winter,semiwinter,and spring types. However,genetic variations underlying the divergence of these ecotypes are largely unknown. Here,we report the global pattern of genetic polymorphisms in rapeseed determined by resequencing a worldwide collection of 991 germplasm accessions.A total of 5.56 and 5.53 million singlenucleotide polymorphisms (SNPs)as Well as 1.86 and 1.92 million InDels were identified by mapping reads to the reference genomes of 'Darmor-bzh'and 'Tapidor,'respectively.We generated a map of allelic drift paths that shows splits and mixtures of the main populations,and revealed an asymmetric evolution of the two subgenomes of B.napus by calculating the genetic diversity and linkage disequilibrium parameters.Selective-sweep analysis revealed genetic changes in genes orthologous to those regulating various aspects of plant development and response to stresses.A genome-wide association study identified SNPs in the promoter regions of FLOWERING LOCUS T and FLOWERING LOCUS C orthologs that corresponded to the different rapeseed ecotype groups. Our study provides important insights into the genomic footprints of rapeseed evolution and flowering-time divergence among three ecotype groups,and will facilitate screening of molecular markers for accelerating rapeseed breeding. | Dezhi Wu Zhe Liang Tao Yan Ying Xu Lijie Xuan Juan Tang Gang Zhou Ulrike Lohwasser Shuijin Hua Haoyi Wang Xiaoyang Chen Qian Wang Le Zhu Antony Maodzeka Nazim Hussain Zhilan Li Xuming Li Imran Haider Shamsi Ghulam Jilani Linde Wu Hongkun Zheng Guoping Zhang Boulos Chalhoub Lisha Shen Hao Yu Lixi Jiang | 2019 | Molecular Plant2019，12，1： | 10 |
| 2 | Detection of Tocopherol in Oilseed Rape (Brassica napus L.) Using Gas Chromatography with Flame Ionization Detector显示文摘The variation among Chinese genotypes of Brassica napus L.for seed tocopherols content and their analysis using gas chromatography has not been comprehensively reported till to date.In the present study,the tocopherol contents of four Chinese genotypes of Brassica napus L.,namely,Gaoyou 605,Zhejiang 619,Zheshuang 758,and Zheshuang 72,were evaluated using three modified sample preparation protocols(P1,P2,and P3) for tocopherol extraction.These methods were distinguished as follows.Protocol one(P1) included the evaporation of solvent after extraction without silylation.Protocol two(P2) followed the direct supernatant collection after overnight extraction without drying and silylation.Protocol three(P3) included trimethylsilylation with N,O-bis(trimethylsilyl) trifluoroacetamide.Genotypic comparison of tocopherol and its isoforms revealed that Gaoyou 605 was dominant over the other genotypes with(140.5±10.5),(316.2± 9.2),and(559.1±24.3) μg g-1 of seed meal α-,γ-,and total(T-) tocopherol,respectively,and a 0.44±0.04 α-to γ-tocopherol ratio.The comparison of the sample preparation protocols,on the other hand,suggests that P3 is the most suitable method for the tocopherol extraction from Brassica oilseeds and for the analysis of tocopherols using gas chromatography flame ionization detector(GC-FID).Trimethylsilylation is the key step differentiating P3 from P1 and P2.Variations detected in tocopherol contents among the Chinese rapeseed(B.napus) genotypes signify the need to quantify a wide range of rapeseed germplasm for seed tocopherol dynamics in short and crop improvement in long. | Nazim Hussain Zahra Jabeen LI Yuan-long CHEN Ming-xun LI Zhi-lan GUO Wan-li Imran Haider Shamsi CHEN Xiao-yang JIANG Li-xi | 2013 | Journal of Integrative Agriculture2013，12，5： | 6 |
| 3 | Interactions of cadmium and aluminum toxicity in their effect on growth and physiological parameters in soybean显示文摘The effect of Al and Cd on the growth, photosynthesis, and accumulation of Al, Cd and plant nutrients in two soybean genotypes were determined using hydroponic culture. There were six treatments: pH 6.5; pH 4.0; pH 6.5+1.0 μmol/L Cd; pH 4.0+1.0 μmol/L Cd; pH 4.0+150 μmol/L Al; pH 4.0+1.0 μmol/L Cd+150 μmol/L Al. The low pH (4.0) and Al treatments caused marked reduction in root length, shoot height, dry weight, chlorophyll content (SPAD value) and photosynthetic rate. Al-sensitive cv. Zhechun 2 accumulated comparatively more Al and Cd in plants than Al-tolerant cv. Liao 1. Compared with pH 6.5, pH 4.0 resulted in significant increase in Cd and Al concentration in plants. Combined application of Cd and Al enhanced their accu-mulation in roots, but caused a reduction in shoots. The concentrations of all 10 nutrients (P, K, Ca, Mg, Fe, Mn, Cu, Zn and B), except Mo were also increased when plants were exposed to pH lower than pH 6.5. Al addition caused a reduction in the con-centration of most nutrients in plant roots and shoots; but K, Mn and Zn in roots were increased. Treatments with Cd alone or together with Al reduced the concentrations of all the plant nutrients in plants. Al-sensitive genotype Zhechun 2 has lower nutrient concentration than Al-tolerant genotype Liao 1. The current findings imply that Al and Cd are synergistic in their effect on plant growth, physiological traits and nutrient uptake. | SHAMSI Imran Haider JILANI Ghulam | 2007 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2007，8，3： | 5 |
| 4 | Synergistic interaction of NaCl and Cd on growth and photosynthetic parameters in soybean genotypes differing in salinity tolerance显示文摘The effects of salinity(50 mmol/L NaCl) and Cd(1 μmol/L CdCl2) as sole and combined on growth and photosyn-thetic parameters were studied using two soybean genotypes,Huachun 18 and NGB.The concentrations of Cd2+,Zn2+,Ca2+,Mg2+,K+ and Na+ were also determined in seeds and pods.Huachun 18 suffered a more serious decrease than NGB in net photosynthetic rate(Pn) in the treatments of salinity stress alone and combined stress(NaCl+Cd),showing that it is relatively sensitive to salinity.The decrease in Pn caused by salt stress in Huachun 18 was mainly due to the reduced total chlorophyll content and photosynthetic efficiency(the ratio of variable fluorescence to maximal fluorescence,Fv/Fm),whereas the decease in NGB was mainly related to reduced stomatal conductance(Gs).The combined stress of both Na and Cd did not induce further decrease in photosynthesis and fluorescence in the two genotypes relative to salt or Cd stress alone.Greater change in the pod concentrations of Zn2+,Ca2+,Mg2+,K+ and Na+ was detected under salt stress for Huachun 18 than for NGB.The results suggested that the interactive effect of NaCl-Cd on growth and nutrient uptake differs between the two soybean genotypes. | SHAMSI Imran Haider | 2007 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2007，8，4： | 3 |
| 5 | 茉莉酸对油菜(Brassica napus L.)受镉毒害的缓解作用(英文)显示文摘目的:本研究目的在于了解:(1)喷施外源茉莉酸对受到镉胁迫油菜的作用;(2)是否茉莉酸能够通过增强气体交换,从而保护受到氧化胁迫的地上部分组织的叶绿体,进而通过减少镉的吸收来维持离子平衡;(3)是否通过喷施茉莉酸来对具有减缓镉毒害效应的抗氧化酶的活性进行调节。创新点:茉莉酸能够调节响应胁迫的抗氧化酶的活性,从而通过保护叶绿体免受活性氧(ROS)伤害而提高光合产物的能力,最大限度地缓解油菜植株受到的镉毒害。方法:(1)叶片气体交换;(2)叶片光合色素分析;(3)丙二醛与抗氧化酶活性分析;(4)营养成分分析;(5)透射电镜亚细胞水平观察。结论:茉莉酸对于植物受镉毒害的缓解作用的机理在于减少叶片中镉的积累,从而减轻氧化胁迫过程中产生的ROS对于膜系统的损害程度。 | Essa ALI Nazim HUSSAIN Imran Haider SHAMSI Zahra JABEEN Muzammil Hussain SIDDIQUI Li-xi JIANG | 2018 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2018，19，2： | 2 |
| 6 | Alleviation of arsenic toxicity by phosphate is associated with its regulation of detoxification,defense,and transport gene expression in barley显示文摘Arsenic(As) contamination in soils has posed a severe threat to safe crop production. The previous studies showed the antagonism between phosphorus(P) and As in plant growth and As uptake, while the mechanisms of alleviating As toxicity by P is not completely clear. Due to the limiting P condition, it is imperative to understand how low P addition can be used to suppress arsenate As(V) uptake and the subsequent mechanisms involved. Thus in this study we investigated the effect of P addition on As uptake, anti-oxidative enzyme activity, and anti-oxidant content, and the relative expression of transport, defense, and detoxification genes using two barley genotypes differing in As toxicity tolerance. P addition significantly reduced As concentration in plant tissues, and caused the great changes in activities of catalase and superoxide dismutase, glutathione content, and the relative expression of examined genes when the plants of the two barley genotypes were exposed to 100 μmol L^(–1) As, with ZDB160(As-tolerant) being much more affected than ZDB475(As-sensitive). The current results show that P addition can alleviate As toxicity by regulating the expression of As transport, defense, and detoxification genes to a greater extent in As tolerance of barley, suggesting the possibility of controlling As uptake and toxicity by applying low amount of P fertilizers in the As-contaminated soils. | Gerald Zvobgo Jonas Lwalaba Wa Lwalaba Tichaona Sagonda James Mutemachani Mapodzeke Noor Muhammad Imran Haider Shamsi ZHANG Guo-ping | 2019 | Journal of Integrative Agriculture2019，18，2： | 2 |
| 7 | 巴基斯坦高校创业教育探究--一项教师视角的案例研究显示文摘作为一个发展中国家,巴基斯坦面临着贫穷、失业等诸多问题,关注高校创业教育并给予实践,鼓励和引导创业,将极大地促进就业率,为发展巴基斯坦国家经济做出巨大贡献。此研究以巴基斯坦两所高校的教师为研究对象,希望通过对巴基斯坦的旁遮普大学和白沙瓦大学的创业教育调研来进一步认识巴基斯坦高校创业教育的现状。目前巴基斯坦高校创业教育存在着诸如体制不均衡、课程改革滞后等问题,提升巴基斯坦创业教育水平需要政府和高校的共同努力。 | Iffat Batool 徐小洲 Imran Haider Shamsi 孙杨 李娜 | 2015 | 现代大学教育2015，31，4： | 2 |
| 8 | 锌和硅对2种水稻基因型的镉毒害及矿质元素转移的影响(英文)显示文摘为探究在镉(Cd)胁迫处理下锌(Zn)和硅(Si)对水稻中矿质元素从根到地上部转移的长期影响,以水稻耐镉基因型秀水110和镉敏感基因型HIPJ 1为供试材料,设置8个处理组合:3个Si处理水平(0,5,15μmol/L Na_2Si O_3·9H_2O)、3个Zn处理水平(0,1,10μmol/L Zn SO_4·7H_2O)和2个Cd处理水平(0,15μmol/L Cd Cl_2),测定其植株生物量、光合强度、抗氧化酶活性、脂质过氧化强度和矿质元素浓度。结果显示,Si(15μmol/L)或Zn(10μmol/L)可有效缓解Cd(15μmol/L)毒害对水稻植株生长的抑制效应,且以Zn的缓解效果更好。在Cd(15μmol/L)胁迫下添加Si(15μmol/L)或Zn(10μmol/L)可有效降低2个水稻基因型叶片中的丙二醛含量,并增强两者叶片中的过氧化氢酶活性,且以在秀水110中的效果更好。单一Zn或Si处理和Zn-Si复合处理均可减少HIPJ 1中的Cd^(2+)从根至地上部的转运。此外,Cd能显著降低水稻植株体内Ca、Mn、Mg等矿质元素的转运。单一Zn处理能够显著促进植物生长,且效果好于单一Si处理与Zn-Si联合处理。但是Zn-Si复合处理对提高Cd胁迫下矿质元素的吸收效果并不显著。 | MAPODZEKE James Mutemachani SAGONDA Tichaona SEHAR Shafaque 张欣 黄雨晴 ZVOBGO Gerald MAODZEKA Antony LWALABA WA LWALABA Jonas SHAMSI Imran Haider | 2018 | 浙江大学学报(农业与生命科学版)2018，44，3： | 2 |
| 9 | 氮肥种类与施量对油菜种子生育酚的影响(英文)显示文摘研究目的:生育酚是菜籽重要的品质指标,氮肥是影响作物生物学与籽粒产量最常用的肥料。这项研究旨在搞清楚氮肥种类与施量对油菜种子生育酚含量与组分的确切影响,以及这种影响在基因型之间的差异。创新要点:这篇论文研究了不同的氮肥种类(硝态氮与铵态氮)与低、中、高施用量对种子生育酚总量与组分的影响,并分析了其中的原因,为通过合理的氮肥施用方案配置,以达到最理想的菜籽生育酚含量或组分提供依据。研究方法:采用盆钵实验控制氮肥施量与流失的精准方法,五种基因型、二种氮肥种类、三档施量水平,三重复控制误差;尝试用气相色谱法检测菜籽生育酚含量的新方法。重要结论:尿素比硝酸氨更有利于菜籽总生育酚、阿尔法生育酚及伽马生育酚的有效形成;提高氮肥施量对于菜籽形成高含量的总生育酚与伽马生育酚非常有效,但对提高菜籽阿尔法生育酚含量的效果却不太明显。 | Nazim HUSSAIN Hui LI Yu-xiao JIANG Zahra JABEEN Imran Haider SHAMSI Essa ALI Li-xi JIANG | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，2： | 0 |
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