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| 1 | Microvascular pathological features and changes in related injury factors in a rat acute blood stasis model显示文摘OBJECTIVE: To examine the microvascular pathological characteristics and changes in related injury factors in a rat model of acute blood stasis.METHODS: A total of 75 Sprague-Dawley rats were divided randomly and equally into a control group and four experimental groups assessed at different times after the induction of stasis(0, 1, 3 or 6 h after stasis)(n = 15). The acute blood stasis model was established through rat tail-vein injection of high-molecular-weight dextran. After Electrocardiograph(ECG) detection at predetermined times(0,1, 3 and 6 h after induction of stasis), the rats were sacrificed and blood and cardiac samples were harvested for analysis. Hematoxylin-eosin(HE) staining and transmission electron microscopy were used for histopathological detection; an enzyme linked immunosorbent assay(ELISA) was used to detect thromboxane B2(TXB2) and 6-keto-prostaglandin F1α(6-Keto-PGF1α) concentrations; a real-time polymerase chain reaction(PCR) reaction system was used to detect intercellular adhesion molecule1(ICAM-1) and vascular cell adhesion molecule1(VCAM-1) m RNA expression; western blotting was used to detect vascular endothelial cadherin(VE-cadherin) protein expression.RESULTS: The ST segment in the ECG showed gradual elevation after induction of stasis and continued elevation at a high level at 3 and 6 h. The HE staining showed changes in myocardial cell necrosis and tissue dissociation after the induction of stasis, along with inflammatory infiltration. Results of transmission electron microscopy showed immediate changes in blood stasis and lumen occlusion in the microvasculature, along with endothelial cell swelling. After the induction of stasis, TXB2 concentrations gradually increased while 6-Keto-PGF1αconcentrations were immediately significantly reduced. The TXB2/6-Keto-PGF1αratio was maintained at a high level. ICAM-1 m RNA expression showed an unstable elevation while VCAM-1 m RNA expression was significantly reduced after the induction of stasis. Compared with the control group, VE-cadherin protein expression increased at 0 and 3 h after the induction of stasis, while no change occurred at 1 and 6 h.CONCLUSION: The pathological manifestations of acute blood stasis are microvascular blood retention, lumen stenosis and even occlusion. The condition is also called 'blood coagulation and weep' in Traditional Chinese Medicine. The blood stasis model resulted in the injury and necrosis of endothelial cells and cardiomyocytes, along with the presence of an imbalance of vasomotor factor levels, platelet activation, and increases in the expression of adhesion molecules and endothelial barrier dysfunction,which corresponds to 'blood failed to nourish' in Traditional Chinese Medicine. | Zhang Junxiu Feng Yu Li Shaodan, Liu Yi Zhang Yin Guo Yunxia Yang Minghui | 2017 | Journal of Traditional Chinese Medicine2017，37，1： | 5 |
| 2 | Changes in nitric oxide,angiotensinⅡ,angiopoietin-like protein 4 mRNA,neuregulin 1 mRNA,and platelet endothelial cell adhesion molecule-1 in rats with acute blood stasis induced by high-molecular-weight dextran显示文摘OBJECTIVE: To investigate the influence of acute blood stasis on nitric oxide(NO), angiotensin Ⅱ(Ang Ⅱ), angiopoietin-like protein 4(ANGPTL4)m RNA, neuregulin 1(NRG-1) m RNA, and platelet endothelial cell adhesion molecule-1(PECAM-1) in rats with stasis induced by high-molecular-weight dextran(HMWD).METHODS: Seventy-five Sprague Dawley rats were divided randomly into five groups(n = 15 in each group): control group, immediate group, 1 h group,3 h group, and 6 h group. A model of acute blood stasis was established via injection of HMWD into the tail vein. After performing electrocardiogram at the predetermined times according to the grouping, we collected blood and cardiac samples for hematoxylin-eosin(HE) staining and histopathological examination via transmission electron microscopy. Enzyme-linked immunosorbent assay was used to detect plasma levels of NO, AngⅡ, and fibrinogen. Real-time polymerase chain reaction was used to detect the expression of ANGPTL4 m RNA and NRG-1 m RNA. Immunohistochemical methods were used to detect PECAM-1 protein expression.RESULTS: The rat model of blood stasis showed blood retention in the capillary lumens. The ST segment showed gradual elevation, and was still elevated at 3 and 6 h after induction of blood stasis.HE staining showed myocardial cell necrosis and dissolution after modeling, along with basement membrane rupture and mitochondrial structural damage. Transmission electron microscopy showed endothelial cell swelling and an increase in absorption vesicles immediately after modeling. Endothelial cell apoptosis was increased at 3 and 6 h after modeling. Cardiac muscle fibers were disordered and intercalated discs were blurred immediately after modeling. There were massive numbers of dissolved cardiac muscle fibers, ruptured basement membranes, and mitochondrial structural damage at 3 and 6 h after modeling. NO plasma concentration was significantly reduced immediately and 1 h after modeling, while it was increased at 3 and 6 h.AngⅡ plasma concentration was decreased immediately after modeling, but increased at 1, 3, and6 h. Fibrinogen plasma concentration was significantly increased at immediate, 1, 3, and 6 h after modeling. PECAM-1 protein expression was obviously increased immediately after modeling, at 1, 6 h was found mild augment. Expression of Ang PTL4 m RNA was increased at immediate, 1, 3, and 6 h after modeling, and was found further augment at 3,and 6 h. Expression of NRG-1 m RNA was increased at immediate, 1, 3, and 6 h after modeling, and the strongest expression was at 1 h.CONCLUSION: The pathological manifestation of acute blood stasis is characterized by microvascular blood retention. Prolonged blood stasis leads to worsening endothelial cell and cardiomyocyte damage, along with imbalances in the expression of vasomotor factors and increased vascular tone.The pathological damage caused by blood stasis also promotes the expression of cell protection factors. | Feng Yu Zhang Junxiu Li Shaodan Liu Yi Zhang Yin Guo Yunxia Yang Minghui | 2017 | Journal of Traditional Chinese Medicine2017，37，6： | 3 |
| 3 | Recent progress in synthesis of lanthanide-based persistent luminescence nanoparticles显示文摘Persistent luminescence nanoparticles(PLNPs)are a kind of phosphors that can remain luminescent for seconds to several days after the stoppage of excitation.Lanthanides show the special capability to largely broaden the emission range and enhance the luminescence intensity of PLNPs due to their dense energy structure and unique electronic configurations.In the past decades,various methods have been developed for the synthesis of lanthanide-based PLNPs with excellent pe rsistent luminescence propertie s,and the lanthanide-based PLNPs are widely studied in areas including biome dicine,energy,and information storage.In this review,we summarized the research progress in the synthe sis of lanthanidebased PLNPs and outline d several typical synthesis methods.We discussed the fundamental concepts of preparation methods as well as the advantages and drawbacks of the typical synthetic approache s.Moreove r,the current challenges and the potential solutions for the development of lanthanide-based PLNP s are also discussed in an attempt to provide strate gies to further improve the optical properties of lanthanide-based PLNPs.We hope this review can contribute to the design of lanthanide-based PLNPs with desired properties and further promote their applications in biomedicine,energy,and information science. | Yurong Wei Chengxu Gong Min Zhao Lei Zhang Shaodan Yang Peixu Li Zhao Ding Quan Yuan Yanbing Yang | 2022 | Journal of Rare Earths2022，40，9： | 2 |
| 4 | Modelling of dynamic contact length in rail grinding process显示文摘栏杆从去支持火车并且指导车轮的过去的火车忍受经常的动态负担。积累的压力集中将向产生折皱引起栏杆的塑料变丑,接触疲劳裂开,也,其它背叛,导致更多的危险地位甚至出轨风险。磨技术的栏杆因此与旋转磨与缺点移动在栏杆上压的石头被发明了。磨工作的如此的栏杆被经验指导,灵活、科学的操作方法缺乏而非科学地指导。与磨联合起来拿磨擦石头的控制,磨过程的栏杆有特征磨擦的不仅表面而且跑的铁路车辆。首先,它的对重要分析在磨擦石头和栏杆之间的接触长度,因为接触长度是一个批评参数测量石头的磨擦能力。而且,它的需要建立铁路车辆联合起来的模型与磨擦石头结合了代表磨擦的栏杆汽车。因此,为接触长度的理论模型基于几何分析被开发。并且精明的模型被改进在磨擦过程期间认为磨擦汽车是动态行为。最后,结果被拿操作参数和结构参数进计算基于模型获得,它对揭示栏杆由联合磨擦机制和铁路车辆系统磨擦的进程合适。 | Shaodan ZHI Jianyong LI A. M. ZAREMBSKI | 2014 | Frontiers of Mechanical Engineering2014，9，3： | 2 |
| 5 | Tea polyphenols inhibit the growth and angiogenesis of breast cancer xenografts in a mouse model显示文摘Objective:To investigate the anti-angiogenic effect of tea polyphenols(TPS)on breast cancer and normal tissues in a mouse model.Methods:Breast cancer was successfully implanted into 48 BALB/c mice,which were then randomly divided into a TP oral gavage group,a TP local injection group,a ginsenoside Rg3 group,and a model control group according to a random number table.The tumor inhibitory rates of each group were calculated,while microvessel density(MVD)and the expression of vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF),and tissue inhibitor of metalloproteinase(TIMP-2)were detected by immunohistochemistry.Results:TPs could inhibit the growth of breast cancer xenografts in the mouse model.The tumor inhibition rates of the TP oral gavage and TP local injection groups were 37.43%and 40.94%,respectively.Compared with the model control group,MVD and VEGF and bFGF expression was downregulated(all P<.05),whereas TIMP-2 expression was elevated in the TP oral gavage and TP local injection groups(P=.015 and P=.032).TPs showed no significant effect on MVD and VEGF and TIMP-2 expression in the heart,brain,and kidney of the mouse model.Conclusion:TPs can restrict the growth of breast cancer by specifically inhibiting the angiogenesis of breast tumor tissue while having little effect on the normal tissue of important organs including the heart,brain,and kidney. | Peng Lv Fengqin Shi Xinyi Chen Li Xu Chong Wang Shaodan Tian Heng Yang Li Hou | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，2： | 2 |
| 6 | Mechanism research on combination of decoction for reinforcing lung Qi and argon helium lancet in treatment of non-small cell lung cancer显示文摘OBJECTIVE: To observe the effect of decoction for reinforcing lung Qi on T-lymphocytic function, interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) in peripheral blood of patients with non-small cell lung cancer after operation with argon helium lancet in order to explore its mechanism.METHODS: A total of 76 patients suffering from non-small cell lung cancer without surgical indication were randomly divided into a treatment group treated with decoction for reinforcing lung Qi and argon helium lancet and a control group treated with argon helium lancet only to observe lymphocytic proliferation, detect the percentage of positive cells in the T-lymphocyte CD28 with flow cytometry and detect the expression of IL-2 and TNF-α in peripheral blood with enzyme linked immunosorbent assay.RESULTS: Proliferation of T-lymphocytes and expression of CD28, IL-2 and TNF-α in peripheral blood after treatment in the treatment group were more obviously strengthened than those before treatment and those in the control group (all P<0.05).CONCLUSION: The mechanism of using decoction for reinforcing lung Qi and argon helium lancet to treat lung cancer may be realized through promoting T-lymphocytic proliferation, up-regulating expression of CD28, IL-2 and TNF-α, and activating T-cells. | Yin Zhang Shaodan Li Ke Cao Yu Feng Xiao Zhang Yueyong Xiao Jie Li | 2013 | Journal of Traditional Chinese Medicine2013，33，3： | 2 |
| 7 | Separation and accumulation of Cu(Ⅱ),Zn(Ⅱ) and Cr(Ⅵ) from aqueous solution by magnetic chitosan modified with diethylenetria-mine显示文摘 | Li Haibo Bi Shaodan Liu Long | 2011 | Desalination2011，278，3： | 1 |
| 8 | Expert consensus statement on diagnosis and treatment of cancer-related depressed mood state based on Chinese medicine显示文摘This consensus statement is organized into six parts:1)Definitions:cancer-related depressed mood state is defined as a group of depressive symptoms,rather than major depressive disorder.Thus,“cancer-related depression”or“depressed mood state”is introduced as standard terminology and associated with the Chinese medicine concept of“yu zheng”(depression syndrome).2)Pathogenesis:factors including psychological stress,cancer pain,cancer fatigue,sleep disorders,surgery trauma,chemotherapy,and radiation therapy are strongly associated with cancer-related depressed mood state.Crucial elements of pathogenesis are cancer caused by depression,depression caused by cancer,and the concurrence of phlegm,dampness,and stasis from constrained liver-qi and spleen deficiency.3)Symptoms:these include core symptoms,psychological symptoms,and somatic symptoms.Depressed mood and loss of interest are the main criteria for diagnosis.4)Clinical evaluation:based on the Mini-International Neuropsychiatric Interview and a numeric rating scale,and taking mood changes during cancer diagnosis and treatment into consideration,a questionnaire can be drafted to distinguish between major depressive disorder and cancer-related depression.The aim is to assist oncology clinicians to identify,treat,and refer patients with cancer-related depression.5)Diagnosis:diagnosis should be based on the Chinese Classification for Mental Disorders(CCMD-3),taking patients’mood changes during diagnosis and treatment into consideration.6)Treatment:treatments for cancer-related depression must be performed concurrently with cancer treatment.For mild depression,non-pharmacologic comprehensive therapies,including psychological intervention,music therapy,patient education,physical activity,and acupuncture,are recommended;for moderate depression,classical Chinese herbal formulas based on syndrome pattern differentiation combined with antidepressants are suggested;for severe depressive symptoms that have progressed to major depressive disorder, patients should be referred to a psychiatric clinician for specialized care. | Shaodan Tian Mei Jia Li Hou Xinyi Chen Dongyun Li Tianwei Guo | 2015 | Journal of Traditional Chinese Medical Sciences2015，2，4： | 1 |
| 9 | An exper- imental study of bubble sliding characteristics in narrow channel 显示文摘 | Shaodan Li Sichao Tan Chao Xu | 2013 | International Journal of Heat and Mass Transfer2013，，57： | 1 |
| 10 | Separation and ac- cumulation of Cu( I ), Zn( lI ) and Cr( V显示文摘 | Li Haibo Bi Shaodan Liu Long | 2011 | Desalination2011，278，3： | 1 |
| 11 | Dynamic changes of beta-amyloid protein deposition in hippocampus of female ovariectomized rats显示文摘BACKGROUND: To evaluate and summarize the effects of cerebral perfusion and vascular reserve on the treatment of SICAS. Recently, research on β-amyloid protein has focused on the regulatory effects of es- trogen or phytoestrogen on its deposition. However, there have been only a few reports on dynamic changes of β-amyloid protein deposition in hippocampus of ovariectomized rats. OBJECTIVE: To measureβ-amyloid protein deposition in the hippocampal formation of ovariectomized rats by using immunohistochemistry; to observe time-dependent dynamic changes. DESIGN: Randomized controlled animal study. SETTING: Third Xiangya Hospital of Central South University. MATERIALS: The experiment was carried out in the Central Laboratory of the Third Xiangya Hospital of Central South University from November 2005 to December 2006. Fifty healthy female Sprague Dawley (SD) rats, weighing (293 ± 10) g, were provided by the Animal Laboratory of Xiangya Medical College, Central South University. All rats had neither a childbearing history nor hepatic or renal disease, or skeletal deformity. β-amyloid protein immunohistochemical kit was provided by Wuhan Boster Company. The ex- periment was in accordance with animal ethics standards. METHODS: All rats were randomly divided into five groups, including normal control group (n = 10), sham operation group (n = 10), and ovariectomized group (n = 30). After anesthesia in the ovariectomized group, the bilateral ovaries were separated and resected. The same volume of fat was resected in the sham operation group. Rats from the normal control group, however, did not receive any surgical treatments. Rats in the normal control group and sham operation group were sacrificed by anesthesia 7 weeks after surgery. Every ten rats from the ovariectomized group was respectively sacrificed at 7, 15, and 30 weeks after surgery. Immunohistochemistry was used to detectβ-amyloid protein deposition in hippocampal sections. Cell counting and gray value measurements served to record the dynamic changes in β-amyloid protein deposi- tion. MAIN OUTCOME MEASURES: ① Morphological changes. ② Positive cell counts from β-amyloid protein stainings and gray value measurements. RESULTS: All 50 rats were involved in the final analysis. ① Morphological changes. β-amyloid-positive cells were detected in the hippocampus of all rats. Biebrich scarlet stained neurites with a swollen cytoplasm. A few β-amyloid-positive cells were observed in all groups 7 weeks after surgery, and plasma and neurites were slightly stained. By 15 weeks after surgery, a number of β-amyloid-positive cells were observed in the ovariectomized group, and plasma and neurites were also slightly stained. By 30 weeks after surgery, how- ever, many β-amyloid-positive cells were observed in the ovariectomized group. These cells were partially aggregated and darkly stained. ② Positive cell counts and gray value of β-amyloid protein in hippocam- pus. At 7 weeks after surgery, cell counts and gray value measurements were not significantly different in the ovariectomized group compared to the sham operation group and normal control group (P > 0.05). Cell counts and gray value measurements were higher in the ovariectomized group by 15 weeks compared to those by 7 weeks in the normal control group, sham operation group and ovariectomized group (P < 0.05). At 30 weeks after surgery, cell counts and gray value measurements were higher in the ovariectomized group compared to the normal control group. In addition, there were significant differences between sham opera- tion group and ovariectomized group at 7 and 15 weeks after operation (P < 0.05-0.01). Cell counts and gray value measurements increased in all groups over time. CONCLUSION: Extended estrogen deficiency in rats can increase β-amyloid protein deposition in the hippocampus and the deposition increases over time. | Huiqing Xie Jianda Zhou Shaodan Sun Xuhong Li Liming Deng Fengmei Li | 2008 | Neural Regeneration Research2008，3，1： | 0 |
| 12 | Target prediction and activity verification for the antidepressant action of Huangqin(Radix Scutellariae Baicalensis)显示文摘OBJECTIVE:To decipher the antidepressant targets and mechanisms of Huangqin(Radix Scutellariae Baicalensis)(RSB)by a novel computational system based on prediction and experimental verification.METHODS:The putative targets of RSB against depression were identified from Traditional Chinese Medicine Systems Pharmacology(TCMSP)and Drug Bank.Next,protein-protein interaction network of the anti-depression targets of RSB were identified,and differentially expressed genes(DEGs)of depression were mined from the NCBI database.Then,Kyoto Encyclopedia of Genes and Genomes and Gene Ontology were used to analysis the common targets.Finally,the selected pathways and functions were verified by experimentation.RESULTS:Thirty active compounds in RSB were predicted with high confidence by TCMSP and DrugBank,and seventy-one DEGs were identified in the GEO database.Besides,eight core target proteins were screened out by descending order of degree value,including ACHE,IL6,SLC6A4,FOS,SLC6A3,MAOB,DPP4,and JUN.These target genes were further found to be associated with pathways involved in neuronal apoptosis,such as pathways in cancer,Toll-like receptor signaling pathway,and TNF signaling.The cell proliferation assay and wound-healing assay results showed that RSB does not affect PC12 cell proliferation and chemotaxis.Unexpectedly,RSB protected PC12 cells from oxidative stress induced by H2O2 via inhibiting autophagy and apoptosis.We revealed significant changes in mice treated with 400 mg/kg RSB compared with the lipopolysaccharide mice.The possible mechanism for the antidepressive action of RSB is by reducing the expression of LC3-B in CA1 neurons.CONCLUSIONS:Our research partially expounds the mechanism of the antidepressant effect of RSB by the combination of network pharmacology prediction and experimental verification.Furthermore,it is also conducive to the application of Traditional Chinese Medicine within modern medicine. | LI Ganggang LU Ye HE Pei ZHANG Shiyue CHENG Yating ZHANG Shaodan PEI Lin | 2021 | Journal of Traditional Chinese Medicine2021，41，6： | 0 |
| 13 | Short-time Fourier Transform Using Odd Symmetric Window Function显示文摘In this paper,a novel time–frequency(TF)analysis method,called the short-time Fourier transform using odd symmetric window function(OSTFT),is proposed using odd symmetric window function to replace the conventional even window function of STFT.Different from conventional STFT acquiring the amplitude maximum at time and frequency centers,OSTFT acquires the minimum amplitude of 0.Hence,OSTFT can obtain a TF representation(TFR)with high TF resolution by utilizing the leaked energy rather than restraining it.It is worth to mention that the proposed OSTFT can vitiate the effect of window size we choose on the TFR obtained.Furthermore,it also has a good performance on signals with complex instantaneous frequencies(IFs),even crossing IFs.Because we just replace the conventional window function of STFT,the time-consuming of the proposed OSTFT is at the same level as the conventional STFT.The effectiveness of proposed OSTFT has been validated on two complex multi-component simulated numerical signals and a signal collected from the brown bat. | Miaofen Li Youmin Liu Shaodan Zhi Tianyang Wang Fulei Chu | 2022 | Journal of Dynamics, Monitoring and Diagnostics2022，1，1： | 0 |
| 14 | The Dynamics of Interorganizational Collaboration in Disaster Management:A Network Study Based on Flood Disasters in China显示文摘Interorganizational collaboration networks have become an important tool for disaster management.However,research on how different organizations can effectively collaborate throughout the entire disaster management process in centralized states such as China is scarce.This study begins to fill this lacuna by investigating interorganizational collaboration in different phases of disaster management and analyzing changes in the structure of the networks constructed during the preparedness and response phases of the 2020 flood disaster in Hubei Province,China.Building on the complex adaptive systems(CAS)theory,we argue that interorganizational collaboration changes dynamically according to its tasks and requirements.In the preparedness phase,interorganizational collaborations primarily follow established plans and choose horizontal selforganized collaboration mechanisms.However,when the urgent information and resource requirements increase in the response phase,many organizations choose vertical mandatory collaboration mechanisms.We found that organizations at the central and provincial levels in China were well positioned to coordinate information and resources and strengthen the interorganizational collaboration and communication that is crucial in disaster management.These findings contribute to the study of interorganizational collaboration networks in disaster management. | Zhichao Li Long Liu Shaodan Liu | 2023 | International Journal of Disaster Risk Science2023，14，6： | 0 |
| 15 | Application of UAV-Based Imaging and Deep Learning in Assessment of Rice Blast Resistance显示文摘Rice blast is regarded as one of the major diseases of rice.Screening rice genotypes with high resistance to rice blast is a key strategy for ensuring global food security.Unmanned aerial vehicles(UAV)-based imaging,coupled with deep learning,can acquire high-throughput imagery related to rice blast infection.In this study,we developed a segmented detection model(called RiceblastSegMask)for rice blast detection and resistance evaluation.The feasibility of different backbones and target detection models was further investigated.RiceblastSegMask is a two-stage instance segmentation model,comprising an image-denoising backbone network,a feature pyramid,a trinomial tree fine-grained feature extraction combination network,and an image pixel codec module.The results showed that the model combining the image-denoising and fine-grained feature extraction based on the Swin Transformer and the feature pixel matching feature labels with the trinomial tree recursive algorithm performed the best.The overall accuracy for instance segmentation of RiceblastSegMask reached 97.56%,and it demonstrated a satisfactory accuracy of 90.29%for grading unique resistance to rice blast.These results indicated that low-altitude remote sensing using UAV,in conjunction with the proposed RiceblastSegMask model,can efficiently calculate the extent of rice blast infection,offering a new phenotypic tool for evaluating rice blast resistance on a field scale in rice breeding programs. | LIN Shaodan YAO Yue LI Jiayi LI Xiaobin MA Jie WENG Haiyong CHENG Zuxin YE Dapeng | 2023 | Rice science2023，30，6： | 0 |
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