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| 1 | 间断或持续PTH刺激对骨及间充质干细胞的不同分化作用显示文摘目的探讨不同甲状旁腺素(PTH)给药情况下及撤药后,骨及间充质干细胞的不同分化作用机制。方法6周龄雄性C57/BL6J小鼠,基因型为SOX9-creERT/Td-Tomato/Osteocalcin-GFP,分为间断PTH刺激及撤药组(PTH 500 μg·kg^-1·d^-1,皮下注射)、持续PTH刺激及撤药组(PTH泵,80 μg·kg^-1·d^-1,速度0.25 μl/h,皮下埋入)、空白对照组及撤药对照组,共6组,每组8只。分别于给药2周及撤药2周时观察小鼠血清中钙离子浓度及骨密度,并取小鼠胫骨骨骼分别进行病理及免疫荧光染色。结果间断PTH刺激组血清钙离子浓度一过性升高,达(1.36±0.03)mmol/L。持续PTH泵刺激组血清中钙离子浓度逐渐升高,48 h达高峰[(2.33±0.03)mmol/L],2周后恢复正常(1.12~1.27 mmol/L)。PTH刺激2周后,间断组[(0.047±0.002)g/cm2]和持续组[(0.046±0.001)g/cm2]骨密度均高于对照组[(0.044±0.001)g/cm2],但间断组和持续组差异无统计学意义。停药2周后,三组间BMD差异无统计学意义。股骨病理可见间断组骨皮质不明显,大量的骨小梁增生,伴少量纤维组织增生;持续PTH刺激大片纤维组织增生,尤以骨皮质内侧显著,撤药后纤维化消失。间断PTH刺激后骨细胞和成骨细胞明显升高,撤药后明显下降;衬里细胞数量骤减,撤药后有所增加;破骨细胞给药及撤药后差异均无统计学意义。持续PTH刺激破骨细胞增多,衬里细胞骤减,撤药后破骨细胞和衬里细胞增加,成骨细胞减少。腓骨干骺端免疫荧光显示,间断PTH刺激组大量未分化的Td-tomato间充质干细胞,撤药后较前明显减少。持续PTH组少量未分化的Td-tomato间充质干细胞增生,撤药后较前减少。结论间断PTH刺激可通过增加未分化的间充质干细胞和成骨细胞数量,将衬里细胞转换为骨细胞和成骨细胞,进而促进骨生成;持续PTH刺激可使少量未分化的间充质干细胞、成骨细胞和骨细胞增生,伴大量纤维化增生和破骨细胞数量骤增,最终可能导致代谢性骨病,撤药后衬里细胞明显增加,或有利于代谢性骨病的缓解。 | 张瀶曦 Deepak H. Balani Sophia Trinh Henry M. Kronenberg 母义明 | 2018 | 中华医学杂志2018，98，10： | 4 |
| 2 | Post‐stroke depression: mechanisms, translation and therapy显示文摘 | Isabelle Loubinoux Golo Kronenberg Matthias Endres Pascale Schumann‐Bard Thomas Freret Robert K. Filipkowski Leszek Kaczmarek Aurel Popa‐Wagner | 2012 | J. Cell. Mol. Med2012，，9： | 3 |
| 3 | Wnt Signaling in the Niche Enforces Hematopoietic Stem Cell Quiescence and Is Necessary to Preserve Self-Renewal In Vivo显示文摘 | Heather E. Fleming Viktor Janzen Cristina Lo Celso Jun Guo Kathleen M. Leahy Henry M. Kronenberg David T. Scadden | 2008 | Cell Stem Cell2008，，3： | 2 |
| 4 | Repeated Multiview Imaging for Estimating Seedling Tiller Counts of Wheat Genotypes Using Drones显示文摘Early generation breeding nurseries with thousands of genotypes in single-row plots are well suited to capitalize on high throughput phenotyping.Nevertheless,methods to monitor the intrinsically hard-to-phenotype early development of wheat are yet rare.We aimed to develop proxy measures for the rate of plant emergence,the number of tillers,and the beginning of stem elongation using drone-based imagery.We used RGB images(ground sampling distance of 3mm pixel-1)acquired by repeated flights(≥2 flights per week)to quantify temporal changes of visible leaf area.To exploit the information contained in the multitude of viewing angles within the RGB images,we processed them to multiview ground cover images showing plant pixel fractions.Based on these images,we trained a support vector machine for the beginning of stem elongation(GS30).Using the GS30 as key point,we subsequently extracted plant and tiller counts using a watershed algorithm and growth modeling,respectively.Our results show that determination coefficients of predictions are moderate for plant count(R^(2)=0:52),but strong for tiller count(R^(2)=0:86)and GS30(R^(2)=0:77).Heritabilities are superior to manual measurements for plant count and tiller count,but inferior for GS30 measurements.Increasing the selection intensity due to throughput may overcome this limitation.Multiview image traits can replace hand measurements with high efficiency(85-223%).We therefore conclude that multiview images have a high potential to become a standard tool in plant phenomics. | Lukas Roth Moritz Camenzind Helge Aasen Lukas Kronenberg Christoph Barendregt Karl-Heinz Camp Achim Walter Norbert Kirchgessner Andreas Hund | 2020 | Plant Phenomics2020，2，1： | 2 |
| 5 | Endogenous Bone Marrow MSCs Are Dynamic, Fate-Restricted Participants in Bone Maintenance and Regeneration显示文摘 | Dongsu Park Joel A. Spencer Bong Ihn Koh Tatsuya Kobayashi Joji Fujisaki Thomas L. Clemens Charles P. Lin Henry M. Kronenberg David T. Scadden | 2012 | Cell Stem Cell2012，，3： | 2 |
| 6 | Milestones of neuronal development in the adult hippocampus显示文摘 | Gerd Kempermann Sebastian Jessberger Barbara Steiner Golo Kronenberg | 2004 | Trends in Neurosciences2004，，： | 2 |
| 7 | Serum microRNA-1 and microRNA-122 are prognostic markers in patients with hepatocellular carcinoma显示文摘 | Verena K?berle Bernd Kronenberger Thomas Pleli J?rg Trojan Esther Imelmann Jan Peveling-Oberhag Martin-Walter Welker Mohammed Elhendawy Stefan Zeuzem Albrecht Piiper Oliver Waidmann | 2013 | European Journal of Cancer2013，，： | 2 |
| 8 | Post-stroke depression:Clinical aspects,epidemiology,therapy and pathophysiology显示文摘 | Kronenberg G Katchanov J Endres M | 2006 | Nervenarzt2006，77，9： | 1 |
| 9 | Regulatory B cells prevent and reverse allergic airway inflammation via FoxP3-positive T regulatory cells in a murine model 显示文摘 | Amu S Saunders SP Kronenberg M | 2010 | J Allergy Clin Immunol2010，125，： | 1 |
| 10 | Post-stroke depression:mechanisms,translation and therapy显示文摘 | Loubinoux I Kronenberg G Endres M | 2012 | J Cell Mol Med2012，16，9： | 1 |
| 11 | Mastoidectomy reconstruction of the posterior wall and obliteration(MAPRO):preliminary results显示文摘 | Kronenberg J Shapira Y Migirov L | | 0，，04： | 1 |
| 12 | Steroids, carbogen or placebo for sudden hearing loss: a prospective double-blind study显示文摘 | U. Cinamon E. Bendet J. Kronenberg | 2001 | European Archives of Oto - Rhino - Laryngology2001，，9： | 1 |
| 13 | Regulatory B cells prevent and reverse allergic airway inflammation via Foxp3 posi tire T regulatory cells in a murine model 显示文摘 | Amu S Saunders SP Kronenberg M | 2010 | J Allergy Clin Im munol2010，125，5： | 1 |
| 14 | Association of plasma bilirubin with coronary heart disease and segregation of bilirubin as a major gene trait: the NHLBI family heart study 显示文摘 | Hunt SC Kronenberg F Eckfeldt JH | 2001 | Atherosclerosis2001，154，3： | 1 |
| 15 | The chondrogenic transcription factor Sox9 is a target of signaling by the parathyroid hormone-related peptide in the growth plate of endochondral bones显示文摘 | Huang W Chung U I Kronenberg H M | 2001 | Proc Natl Acad Sci U S A2001，98，1： | 1 |
| 16 | Low apolipoprotein A-Ⅳ plasma concentration in men with coronary artery disease显示文摘 | Stuhlinger M Trenkwalder E | 2000 | J Am Coll Cardiol2000，36，3： | 1 |
| 17 | A safery study of albumex 5, a human albumin solution produced by ion exchange chromatography显示文摘 | Wolf M Kronenberg H Dodds A | 1996 | Vax Sang1996，70，4： | 1 |
| 18 | Influence of leisure time physical activity and television watching on atherosclerosis risk factors in the NHLBI Family Heart Study显示文摘 | Kronenberg F Pereira MA Schmitz MK | 2000 | Atherosclerosis2000，153，2： | 1 |
| 19 | 显示文摘 | Kronenberg M | 1994 | Curr Opin Immunol1994，6，： | 1 |
| 20 | Electrical stimulation levels and electrode impedance values in children using the Med-El Combi 40 + cochlear implant:a one year follow-up显示文摘 | Henkin Y Kaplan-Neeman R Kronenberg J | 2005 | J Basic Clin Physiol Pharmacol2005，16，： | 1 |
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