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| 1 | Isolation of bacteria from fermented food and grass carp intestine and their efficiencies in improving nutrient value of soybean meal in solid state fermentation显示文摘Background: Soybean meal is an excellent and cost-effective protein source; however, its usage is limited in the piglet due to the presence of anti-nutritional factors and the antigens glycinin and β-conglycinin. The objective of the current study was to screen and select for bacteria that can be efficiently adopted to ferment soybean meal in order to solve this problem.Results: Bacteria were isolated from fermented soy foods and the grass carp intestine, and strains selected for high protease, cellulase and amylase activities. The isolated bacteria were characterized as Bacillus cereus, Bacillus subtilis and Bacilus amyloliquefacien, respectively. Fermentation with food-derived Isolate-2 and fish-derived F-9 increased crude protein content by 5.32% and 8.27%, respectively; improved the amino acid profile by increasing certain essential amino acids, broke down larger soy protein to 35 k Da and under, eliminated antigenicity against glycinin and β-conglycinin, and removed raffinose and stachyose in the soybean meal following a 24-h fermentation.Conclusions: Our results suggest these two B. amyloliquefaciens bacteria can efficiently solid state ferment soybean meal and ultimately produce a more utilizable food source for growing healthy piglets. | Samantha Medeiros Jingjing Xie Paul W.Dyce Hugh Y.Cai Kees De Lange Hongfu Zhang Julang Li | 2018 | Journal of Animal Science and Biotechnology2018，9，3： | 12 |
| 2 | Characterization and validation of somatic mutation spectrum to reveal heterogeneity in gastric cancer by single cell sequencing显示文摘Gastric cancer(GC) is a highly heterogeneous disease with multiple cellular types and poor prognosis.However, the cellular evolution and molecular basis of GC at the individual intra-tumor level has not been well demonstrated. We performed single-cell whole exome sequencing to detect somatic singlenucleotide variants(SNVs) and significantly mutated genes(SMGs) among 34 tumor cells and 9 normal cells from a patient with GC. The Complete Prediction for Protein Conformation(CPPC) approach directly predicting the folding conformation of the protein 3D structure with Protein Folding Shape Code, combined with functional experiments were used to confirm the characterization of mutated SMGs in GC cells. We identified 201 somatic SNVs, including 117 non-synonymous mutations in GC cells. Further analysis identified 24 significant mutated genes(SMGs) in single cells, for which a single amino acid change might affect protein conformation. Among them, two genes(CDC27 and FLG) that were mutated only in single cells but not in the corresponding tumor tissue, were recurrently present in another GC tissue cohort, and may play a potential role to promote carcinogenesis, as confirmed by functional characterization. Our findings showed a mutational landscape of GC at intra-tumor level for the first time and provided opportunities for understanding the heterogeneity and individualized target therapy for this disease. | Lihua Peng Rui Xing Dongbing Liu Li Bao Wenxiang Cheng Hongyi Wang Yuan Yu Xiaofeng Liu Lu Jiang Yan Wu Zhongxue An Qiaoyi Liang Ryong Nam Kim Young Kee Shin Huanming Yang Jian Wang Jun Yu Xiuqing Zhang Xun Xu Jiaan Yang Kui Wu Shida Zhu Youyong Lu | 2019 | Science Bulletin2019，64，4： | 3 |
| 3 | The Role of Social Media in Providing Crisis Information in China:A Critical Evaluation of the Tianjin Fire Incident显示文摘This paper focusses on the information asymmetry in crisis news after a serious incident in Tianjin, China, in 2015. The incident caused enormous damage and resulted in societal unrest because of the lack of reliable information from the formal media channels. Social media — micro blogs —played a major role in reporting on crisis situations. We divided netizens(i.e., the citizens of the net)into high and low types according to their information-critical level to the crisis news. The data shows information deterioration on the crisis news, related to the netizens' information-critical level. For the traditional media there is the opportunity to use information quality distortion to make more marginal profits. This is possible only if the citizens' information stays under a certain quality level. The result is overprovision of low quality news and high quality news driven out of the market, whereupon adverse selection(i.e., a lack of symmetric information) appears. However, by adopting a process view, we found self-correcting mechanism(i.e., dying out of rumors) of the social media communities in China.We provided a agent-base model and simulation to show that the more media exist in the market, the faster speed of the information deterioration, but also the capacity to ‘discuss' rumors. | Xiangfei LI Kees BOERSMA | 2017 | Journal of Systems Science and Information2017，8，6： | 2 |
| 4 | Photolu- minescence properties of CeO2 : Eu:+ nanoparticles synthesized by a sol-gel method显示文摘 | Ling Li Hyun Kyoung Yang Byung Kee Moon | 2009 | Journal of Physical Chemistry C2009，113，2： | 1 |
| 5 | Hopxand Hdac2 interact to modulate Gata4 acetylation and embryoniccardiac myocyte proliferation显示文摘 | Trivedi CM Zhu W Wang Q Jia C Kee HJ Li L | 2010 | Dev Cell2010，19，3： | 1 |
| 6 | Seroepidemiology of hepatitis B virus infection among adults in Singapore: A 12-year review显示文摘 | Li Wei Ang Jeffery Cutter Lyn James Kee Tai Goh | 2013 | Vaccine2013，，1： | 1 |
| 7 | Efficient silencing of the multicopy DUX4 gene by ABE-mediated start codon mutation in human embryos显示文摘The Dux gene plays an important role in mouse embryo development(De Iaco et al.,2017;Hendrickson et al.,2017;Whiddon et al.,2017).It participates in the maternal-to-zygotic transition(MTZ),which mainly includes maternal transcript degradation and zygotic genome activation(ZGA)and is the key process for embryonic development.Approximately 11-100 copies of the DUX4 gene form a tandem cluster in the human genome(Snider et al.,2009,2010). | Yubing Liu Xinmei Lu Min Ye Ling Wang Rongxin Tang Zhiyong Yang Bongkoch Turathum Chenchen Liu Yujing Xue Menghua Wu Yannan Yang Ermeng Gao Di Zhang Feng Yang Keh-Kooi Kee Xingxu Huang Guanglei Li Ri-Cheng Chian | 2022 | Journal of Genetics and Genomics2022，49，10： | 1 |
| 8 | Application of static and dynamic liquid-phase microextraction in the determination of polycyclic aromatic hydrocarbons显示文摘 | Li Hou Hian Kee Lee | 2002 | Journal of Chromatography A2002，，1： | 1 |
| 9 | Developments in single-drop microextraction显示文摘 | Li Xu Chanbasha Basheer Hian Kee Lee | 2007 | J Chromatogr A2007，1152，12： | 1 |
| 10 | In Vitro and In Vivo Toxicity of Garcinia or Hydroxycitric Acid: A Review显示文摘 | Li Oon Chuah Swee Keong Yeap Wan Yong Ho Boon Kee Beh Noorjahan Banu Alitheen Debprasad Chattopadhyay | 2012 | Evidence-Based Complementary and Alternative Medicine2012，，： | 1 |
| 11 | A 193-amin acid fragment of the SARS coronavirus S protein efficiently binds angiotensin-converting enzyme 显示文摘 | Swee Kee Wong Wenhui li Michael J | 2004 | J Biol chem2004，279，5： | 1 |
| 12 | Determination of pesticides in soil by liquid-phase microextraction and gas chromatography–mass spectrometry显示文摘 | Li Hou Hian Kee Lee | 2004 | Journal of Chromatography A2004，，1： | 1 |
| 13 | Screening of synthetic PDE-5 inhibitors and their analogues as adulterants: Analytical techniques and challenges显示文摘 | Dhavalkumar Narendrabhai Patel Lin Li Chee-Leong Kee Xiaowei Ge Min-Yong Low Hwee-Ling Koh | 2013 | Journal of Pharmaceutical and Biomedical Analysis2013，，： | 1 |
| 14 | Echocardiography in serial evaluation of left ventricular systolic and diastolic function:importance of image acquisition,quantitation,and physiologic variability in clinical and investigational applications显示文摘 | Kuecherer HF Kee LI Modin G | 1991 | J Am Soc Echocardiogr1991，4，3： | 1 |
| 15 | Developments in single-drop microextraction 显示文摘 | Li Xu Chanbasha Basheer Hian Kee Lee | 2007 | J Chromatogr A2007，1152，12： | 1 |
| 16 | Cucurbitacin B induces apoptosis and S phase cell cycle arrest in BEL-7402 human hepatocellular carcinoma cells and is effective via oral administration显示文摘 | Kin Tak Chan Fan Yan Meng Qian Li Cheong Yip Ho Tsz Shan Lam Yu To Wai Him Lee Miao Li Kee Hung Chu Melvin Toh | 2010 | Cancer Letters2010，，1： | 1 |
| 17 | Supercritical Fluid Chromatographic Separation of 11 Priority Phenols Using Chlorodifluoromethane as Modfier 显示文摘 | Ong Chye Peng Lee Hian Kee Li San Fong Yau | 1992 | Journal of Chromatographic Science1992，30，8： | 1 |
| 18 | Screening of synthetic PDE-5 inhibitors and their analogues as adulterants: Analytical techniques and challenges显示文摘 | Dhavalkumar Narendrabhai Patel Lin Li Chee-Leong Kee Xiaowei Ge Min-Yong Low Hwee-Ling Koh | 2013 | Journal of Pharmaceutical and Biomedical Analysis2013，，： | 1 |
| 19 | Determination of Pesticides in Soil by Liquid Phase Microextraction and Gas Chromatography-mass Spectrometry 显示文摘 | Li Hou Hian Kee Lee | 2004 | Journal of Chromatography A2004，1038，12： | 1 |
| 20 | Determination of pesticides in soil by liquid-phase microextraction and gas chromatography mass spectrometry显示文摘 | HOU LI LEE HIAN KEE | 2004 | J Chromat A2004，1038，： | 1 |
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