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| 1 | BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity显示文摘基础编辑() ,包含 cytidine 脱氨基酶和催化地有缺点的 Cas9,广泛地被用来执行基础编辑。然而,狭窄的编辑窗口是限制它的实用程序。这里,我们开发了依照到由熔化 10 的 U (T) 范围(加) 的更大的 C 为招募 GCN4 肽拷贝到 nCas9 (D10A ) 的编程作为基础编辑命名的一种新编辑技术 scFv-APOBEC-UGI-GB1 到目标地点。新系统完成与一扇拓宽的窗户的基础编辑,导致一个增加的指向染色体的范围。有趣地,新系统很产出更少讨厌的 indels 和 non-C-to-T 变换。我们也通过站 codons (iSTOP ) 的正式就职越过整个染色体在基因混乱表明了它的潜在的使用。一起拿,加系统与编辑窗口和提高的忠实增加提供一个新编辑工具。 | Wen Jiang Songjie Feng Shisheng Huang Wenxia Yu Guanglei Li Guang Yang Yajing Liu Yu Zhang Lei Zhang Yu Hou Jia Chen Jieping Chen Xingxu Huang | 2018 | Cell Research2018，28，8： | 20 |
| 2 | Horizontal-Based Orchestration for Multi-Domain SFC in SDN/NFV-Enabled Satellite/Terrestrial Networks显示文摘It's promising to use Software-Defined Networking(SDN) and Network Functions Virtualization(NFV) to integrate satellite and terrestrial networks. To construct network service function chains in such a multi-domain environment, we propose a horizontal-based Multi-domain Service Function Chaining(Md-SFC) orchestration framework. In this framework, multi-domain orchestrators can coordinate with each other to guarantee the end-to-end service quality. Intra-domain orchestrators also coordinate SDN controllers and NFV management components to implement intra-domain service function chains. Based on this, we further propose a heuristic SFC mapping algorithm with a cooperative inter-domain path calculation method to map service function chains to infrastructures. In this method, master multi-domain orchestrator and intra-domain orchestrators coordinate to select proper inter-domain links. We compare the cooperative method with a naive uncooperative way that domains' topology information is provided to the master multi-domain orchestrator and it calculates the shortest inter-domain path between intra-domain service function chains directly. Simulation results demonstrate that our solution is feasible. It is able to construct end-to-end performance guaranteed service function chain by horizontal-based cooperation. The cooperative inter-domain path calculation method decreasesthe mapping load for the master orchestrator and gets the same end-to-end performance. | Guanglei Li Huachun Zhou Bohao Feng Guanwen Li Qi Xu | 2018 | China Communications2018，15，5： | 11 |
| 3 | Back analysis of mechanical parameters of roller compacted concrete dam显示文摘In view of the diversity and complexity of mechanical parameters of roller compacted concrete dam(RCCD),the uniform design method,partial least-squares regression(PLS)and least squares support vector machine(LSSVM)were applied to the back analysis of RCCD with the use of the complex nonlinear relationship between dam mechanical parameters and dam displacements.During the process of back analysis,the initial samples of parameters were designed with uniform design method.Then,a transversely isotropic model of RCCD was established by MSC.Marc software.Through this model,training samples of LSSVM model could be obtained.And then,the complex nonlinear relationship between relative values of hydraulic components of dam displacements and mechanical parameters was established.Finally,actual relative values of dam hydraulic components are isolated from the measured data of dam displacements by using PLS.By inputting the isolated relative values into LSSVM model,the back analysis values of RCCD mechanical parameters can be obtained.The example analysis showed that mechanical parameters obtained by the above-mentioned back analysis method are reasonable,and the back analysis method is feasible. | GU ChongShi 1,2,3,LI Bo 1,XU GuangLei 4 &YU Hong 1,2,3 1 State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering,Hohai University,Nanjing 210098,China 2College of Water Conservancy Hydropower,Hohai University,Nanjing 210098,China 3National Engineering Research Center of Water Resources Engineering Safety,Efficient Utilization and Nanjing 210098,China 4 Nanjing Hydraulic Research Institute,Nanjing 210029,China | 2010 | Science China(Technological Sciences)2010，53，3： | 8 |
| 4 | Highly efficient and precise base editing in discarded human tripronuclear embryos显示文摘 | Guanglei Li Yajing Liu Yanting Zeng Jianan Li Lijie Wang Guang Yang Dunjin Chen Xiaoyun Shang Jia Chen Xingxu Huang Jianqiao Liu | 2017 | Protein & Cell2017，8，10： | 8 |
| 5 | Gene editing and its applications in biomedicine显示文摘The steady progress in genome editing, especially genome editing based on the use of clustered regularly interspaced short palindromic repeats(CRISPR) and programmable nucleases to make precise modifications to genetic material, has provided enormous opportunities to advance biomedical research and promote human health. The application of these technologies in basic biomedical research has yielded significant advances in identifying and studying key molecular targets relevant to human diseases and their treatment. The clinical translation of genome editing techniques offers unprecedented biomedical engineering capabilities in the diagnosis, prevention, and treatment of disease or disability. Here, we provide a general summary of emerging biomedical applications of genome editing, including open challenges. We also summarize the tools of genome editing and the insights derived from their applications, hoping to accelerate new discoveries and therapies in biomedicine. | Guanglei Li Xiangyang Li Songkuan Zhuang Liren Wang Yifan Zhu Yangcan Chen Wen Sun Zeguang Wu Zhuo Zhou Jia Chen Xingxu Huang Jin Wang Dali Li Wei Li Haoyi Wang Wensheng Wei | 2022 | Science China(Life Sciences)2022，65，4： | 8 |
| 6 | Cadmium pollution enhanced ozone damage to winter wheat:biochemical and physiological evidences显示文摘Combined effects of ozone (O3) and cadmium (Cd) on growth and physiology of winter wheat (Triticum aestivum L.cv.JM22) were determined.Wheat plants were grown without or with Cd and exposed to charcoal-filtered air (< 10 ppb O3) or elevated O 3 (80 ± 5 ppb,7 hr/day) for 20 days.Results showed that O 3 considerably depressed light saturated net photosynthetic rate (-20%),stomatal conductance (-33%),chlorophyll content (-33%),and total biomass (-29%) without Cd.The corresponding decreases were further enhanced by 45%,56%,60% and 59%,respectively with Cd,indicating a synergistic effect of O3 and Cd on wheat.Ozone significantly increased the activity of superoxide dismutase (46%),catalase (48%) and peroxidase (56%).However,great increases in malondialdehyde (MDA) content (2.55 folds) and intercellular CO2 concentration (1.13 folds) were noted in O3 +Cd treatment compared to control.Our findings demonstrated that the increased anti-oxidative activities in wheat plants exposed to O3 +Cd might not be enough to overcome the adverse effects of the combination of both pollutants as evidenced by further increase in MDA content,which is an important indicator of lipid peroxidation.Precise prediction model on O3 damages to crop should be conducted to ensure agricultural production security by considering environmental constraints in an agricultural system in peri-urban regions. | Yong Li Caihong Li Yanhai Zheng Guanglei Wu Tana Wuyun Hong Xu Xinhua He Gaoming Jiang | 2011 | Journal of Environmental Sciences2011，23，2： | 7 |
| 7 | Closed reduction external fixator fixation versus open reduction internal fixation in the patients with Bennett fracture dislocation显示文摘 | Li Zhongzhe Guo Yang Tian Wen Tian Guanglei | 2014 | Chinese Medical Journal2014，，22： | 6 |
| 8 | Enhancing prime editing by Csy4-mediated processing of pegRNA显示文摘Dear Editor,The most advanced prime editor 3(PE3)system comprises the editor,a fusion protein of Cas9 H840A nickase and mutant reverse transcriptase(RTase)(hereafter termed NMRT),a prime editing guide RNA(pegRNA)and an alternative single-guide RNA(sgRNA).1 The pegRNA contains a primer binding site(PBS)and a reverse transcription(RT)template for introducing new genetic information1(Fig.1a;Supplementary information,Fig.S1a).We noted that the PBS,which is generally 10–16 nt at the 3′end of pegRNA,is complementary to part of the spacer at the 5′end of pegRNA,and their annealing is expected to cause pegRNA circularization,which can potentially hamper editing(Fig.1a;Supplementary information,Fig.S1b,c). | Yao Liu Guang Yang Shuhong Huang Xiangyang Li Xin Wang Guanglei Li Tian Chi Yulin Chen Xingxu Huang Xiaolong Wang | 2021 | Cell Research2021，31，10： | 5 |
| 9 | Polyaniline-coated selenium/carbon composites encapsulated in graphene as efficient cathodes for Li-Se batteries显示文摘 | Biwu Wang Jingjing Zhang Zhigang Xia Meiqiang Fan Chunju Lv Guanglei Tian Xiaona Li | 2018 | Nano Research2018，11，5： | 5 |
| 10 | Efficient base editing in G/C-rich regions to model androgen insensitivity syndrome显示文摘Dear Editor, Most disease-associated genomic mutations are base substitutions and approximately half of pathogenic human single nucleotide polymorphisms (SNPs)are related to C-to-T substitutions in the ClinVar database.Base editors (BEs),which combine Casg-DIOA nickase and APOBEC (apolipoprotein B mRNA editing enzyme,catalytic polypeptide-like)or AID (activation-induced deaminase)cytidine deaminase family members, have been successfully applied to mediate C-to-T conversion in vitro and in vivo, providing a powerful tool to model or repair diseaserelated human SNPs. | Jianan Li Zhen Liu Shisheng Huang Xiao Wang Guanglei Li Yuting Xu Wenxia Yu Shanshan Chen Yu Zhang Hanhui Ma Zunfu Ke Jia Chen Qiang Sun Xingxu Huang | 2019 | Cell Research2019，29，2： | 3 |
| 11 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 12 | Current advances of transition metal dichalcogenides in electromagnetic wave absorption:A brief review显示文摘Transition metal dichalcogenides(TMDs)show great advantages in electromagnetic wave(EMW)absorption due to their unique structure and electrical properties.Tremendous research works on TMD-based EMW absorbers have been conducted in the last three years,and the comprehensive and systematical summary is still a rarity.Therefore,it is of great significance to elaborate on the interaction among the morphologies,structures,phases,components,and EMW absorption performances of TMD-based absorbers.This review is devoted to analyzing TMD-based absorbers from the following perspectives:the EMW absorption regulation strategies of TMDs and the latest progress of TMD-based hybrids as EMW absorbers.The absorption mechanisms and component-performance dependency of these achievements are also summarized.Finally,a straightforward insight into industrial revolution upgrading in this promising field is proposed. | Shijie Zhang Jiying Li Xiaotian Jin Guanglei Wu | 2023 | International Journal of Minerals,Metallurgy and Materials2023，30，3： | 3 |
| 13 | Tuning microwave absorption properties of Ti_(3)C_(2)T_(x) MXene-based materials:Component optimization and structure modulation显示文摘The current electromagnetic environment brings a growing demand for efficient microwave absorption(MA)materials.Ti_(3)C_(2)T_(x) MXene,one of the 2D transition-metal carbides,is considered to be a promising MA material owing to its superior dielectric properties and structural processability.In order to further improve the MA performance and environmental adaptability of Ti_(3)C_(2)T_(x) MXene,Ti_(3)C_(2)T_(x) MXene-based MA materials enhanced by composition and structure design have been extensively studied and the regu-lation ideas for its MA properties can be outlined as component optimization and structure manipulation strategies based on the microwave absorption mechanism.Herein,we briefly introduced the microwave absorption mechanism and focused on the design strategies of Ti_(3)C_(2)T_(x) MXene-based MA materials based on recent advances.In addition,the prospects of Ti_(3)C_(2)T_(x) MXene-based MA materials were also discussed. | Ming Chang Qingyu Li Zirui Jia Wanru Zhao Guanglei Wu | 2023 | Journal of Materials Science & Technology2023，，17： | 3 |
| 14 | Generation of isogenic single and multiplex gene knockout mice by base editing-induced STOP显示文摘Although CRISPR/Cas9 has been widely used to generate knockout mice, two major limitations remain:the founders usually carry a mixture of genotypes, and mosaicism harboring multiple genotypes.Therefore, it takes a long time to get homozygous mutants. Recently developed base editing(BE) system,which introduces C-to-T conversion without double strand DNA cleavage, has been used to introduce artificial stop codons(i-STOP) to prematurely terminate translation, providing a cleaner strategy for genome engineering. Using this strategy, we generated CD160 KO and VISTA/CD160 double KO mice by microinjection of a single sg RNA targeting CD160 and a mixture of sg RNAs targeting VISTA and CD160,respectively. The BE system induced STOP efficiently in mouse embryos and consequently in founder mice without detectable off-target. Most interestingly, the majority of the mutants harbor same genetic modifications, indicating we generated isogenic single and multiplex gene mutant mice by BE-induced STOP. We also obtained homozygous mutant mouse in F1 mice, demonstrating the accelerated strategy in generating animal models. | Guang Yang Tianyu Zhu Zongyang Lu Guanglei Li Hao Zhang Songjie Feng Yajing Liu Jianan Li Yu Zhang Jia Chen Xuejiang Guo Xingxu Huang | 2018 | Science Bulletin2018，63，17： | 2 |
| 15 | Microstructure evolution and mechanical behavior of Ni-based single crystal superalloy joint brazed with mixed powder at elevated temperature显示文摘Brazing of a Ni-based single crystal superalloy has been investigated with the additive Ni-based superalloy and filler Ni–Cr–W–B alloy at 1260℃, and attentions were paid to the microstructure evolution during brazing and the stress-rupture behavior at 980℃ of such brazed joints after homogenization. Microstructure in the brazed joint generally includes brazing alloy zone(BAZ), isothermally solidified zone(ISZ) and diffusion affected zone(DAZ). Microstructure evolution during this brazing process is discussed at the heating stage, the holding stage and the cooling stage respectively, according to the diffusion path of B atoms. Initially well-distributed γ’/γ’ microstructure in the homogenized bonded zone after heat treatment and substantial γ’ rafts enhance the post-brazed joint to obtain a stress-rupture lifetime of more than 120 h at 980℃/250 MPa. On the other hand, the decreased stress-rupture behavior of post-brazed joint, compared with parenting material, is ascribed to the presence of inside brazing porosity and stray grain boundary, which not only reduces the effective loading-carrying area but also offers preferential sites for creep vacancy aggregation to further soften stray grain boundary. And finally an early fracture of these post-brazed joints through the intergranular microholes aggregation and growth mode under this testing condition was observed. | Guanglei Wang Yuan Sun Xinguang Wang Jide Liu Jinlai Liu Jinguo Li Jinjiang Yu Yizhou Zhou Tao Jin Xudong Sun Xiaofeng Sun | 2017 | Journal of Materials Science & Technology2017，33，10： | 2 |
| 16 | Efficient silencing of the multicopy DUX4 gene by ABE-mediated start codon mutation in human embryos显示文摘The Dux gene plays an important role in mouse embryo development(De Iaco et al.,2017;Hendrickson et al.,2017;Whiddon et al.,2017).It participates in the maternal-to-zygotic transition(MTZ),which mainly includes maternal transcript degradation and zygotic genome activation(ZGA)and is the key process for embryonic development.Approximately 11-100 copies of the DUX4 gene form a tandem cluster in the human genome(Snider et al.,2009,2010). | Yubing Liu Xinmei Lu Min Ye Ling Wang Rongxin Tang Zhiyong Yang Bongkoch Turathum Chenchen Liu Yujing Xue Menghua Wu Yannan Yang Ermeng Gao Di Zhang Feng Yang Keh-Kooi Kee Xingxu Huang Guanglei Li Ri-Cheng Chian | 2022 | Journal of Genetics and Genomics2022，49，10： | 1 |
| 17 | Reliability Analysis of Random Fuzzy Repairable Series System 显示文摘 | Liu Ying Li Xiaozhong Yang Guanglei | 2010 | Fuzzy Information and Engineering2010，，1： | 1 |
| 18 | Oral administration of dibutyryl adenosine cyclophosphate improved growth performance in weaning piglets by enhancing lipid fatty acids metabolism显示文摘Dibutyryl adenosine cyclophosphate(dbc AMP-Ca), an analog of cyclic adenosine monophosphate(c AMP), plays greater roles in regulating physiological activities and energy metabolism than c AMP. The aim of this study was to investigate the effect of oral administration of dbc AMP-Ca on growth performance and fatty acids metabolism in weaning piglets. A total of 14 early weaning piglets(7 ± 1 d of age,3.31 ± 0.09 kg, Landrace ? Large White ? Duroc) were randomly divided into 2 groups: control group and dbc AMP-Ca group, and the piglets received 7 m L of 0.9% Na Cl or 1.5 mg dbc AMP-Ca dissolved in 7 m L of 0.9% Na Cl per day for 10 d, respectively. The results showed that the average daily gain(ADG)increased by 109.17%(P < 0.05) in the dbc AMP-Ca group compared with the control group. Besides,dbc AMP-Ca significantly decreased blood high density lipoprotein cholesterol(HDLC) concentration(P < 0.05) and significantly increased blood low density lipoprotein cholesterol(LDLC) concentration(P < 0.05) compared with the control group. Further, liver C18:2 n6 t content significantly increased in dbc AMP-Ca group(P < 0.05) compared with the control group. With the increase of C18:2 n6 t content,the m RNA expression levels of peroxisome proliferator-activated receptor a(PPARa) and hormone sensitive glycerol three lipase(HSL), of which genes are related to lipid metabolism, were also significantly increased(P < 0.05 or P < 0.01). All of the results indicated that dbc AMP-Ca improved the ADG, which was probably done by regulating fatty acids metabolism in the liver of weaning piglets. | Guanya Li Ling Chang Guanglei Zhang Zehe Song Dan Wan Chunyan Xie Hong Wang Zhiyong Fan | 2018 | Animal Nutrition2018，，3： | 1 |
| 19 | Ecological risk of invasive plants in Henan Province based on PRA显示文摘 | YU Guangli LI Wenzeng CHEN Guanglei | 2009 | Journal of Henan Normal University: Natural Science2009，4，： | 1 |
| 20 | A new route to prepare supported nickel phosphide/silica-alumina hydrotreating catalysts from amorhous alloys显示文摘 | Song Limin Li Wei Wang Guanglei | 2007 | Catalysis Today2007，125，34： | 1 |
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