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| 1 | Review of current diagnostic methods and advances in Helicobacter pylori diagnostics in the era of next generation sequencing显示文摘Helicobacter pylori(H.pylori)infection is highly prevalent in the human population and may lead to severe gastrointestinal pathology including gastric and duodenal ulcers,mucosa associated tissue lymphoma and gastric adenocarcinoma.In recent years,an alarming increase in antimicrobial resistance and subsequently failing empiric H.pylori eradication therapies have been noted worldwide,also in many European countries.Therefore,rapid and accurate determination of H.pylori’s antibiotic susceptibility prior to the administration of eradication regimens becomes ever more important.Traditionally,detection of H.pylori and its antimicrobial resistance is done by culture and phenotypic drug susceptibility testing that are cumbersome with a long turn-around-time.Recent advances in diagnostics provide new tools,like real-time polymerase chain reaction(PCR)and line probe assays,to diagnose H.pylori infection and antimicrobial resistance to certain antibiotics,directly from clinical specimens.Moreover,high-throughput whole genome sequencing technologies allow the rapid analysis of the pathogen’s genome,thereby allowing identification of resistance mutations and associated antibiotic resistance.In the first part of this review,we will give an overview on currently available diagnostic methods for detection of H.pylori and its drug resistance and their implementation in H.pylori management.The second part of the review focusses on the use of next generation sequencing technology in H.pylori research.To this end,we conducted a literature search for original research articles in English using the terms“Helicobacter”,“transcriptomic”,“transcriptome”,“next generation sequencing”and“whole genome sequencing”.This review is aimed to bridge the gap between current diagnostic practice(histology,rapid urease test,H.pylori culture,PCR and line probe assays)and new sequencing technologies and their potential implementation in diagnostic laboratory settings in order to complement the currently recommended H.pylori management guidelines and subsequently improve public health. | Daniel Pohl Peter M Keller Valentine Bordier Karoline Wagner | 2019 | World Journal of Gastroenterology2019，25，32： | 15 |
| 2 | Identification of CD13,CD107a,and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-de-pendent upregulation显示文摘Using two-colour flow cytometry >200 antibodies submitted to the 8th International Workshop of Human Leukocyte Differentiation Antigens (HLDA8) have been analyzed for their reactivity with resting and activated CD203c+ basophils. Four antibodies either non-reactive or weakly reactive with resting basophils exhibited an increased reactivity with basophils activated by anti-IgE-mediated cross-linking of the high affinity IgE receptor (FcεRI). These include antibod- ies against CD164 (WS-80160, clone N6B6 and WS-80162, clone 67D2), as well as two reagents with previously unknown specificities that were identified as CD13 (WS-80274, clone A8) and CD107a (WS-80280, clone E63-880). The activation patterns followed either the “CD203c-like” or “CD63-like” activation profile. The CD203c profile is characterized by a rapid and significant upregulation (of CD13, CD164, and CD203c), reaching maximum levels after 5- 15 min of stimulation. The phosphoinositide-3-kinase (PI3K)-specific inhibitor wortmannin inhibited the upregulation of these markers whereas 12-O-tetradecanoyl-phorbol-13-acetate (TPA) induced a rapid and FcεRI-independent upregulation within 1-2 min. In the CD63 profile, maximum upregulation (of CD63 and CD107a) was detected only after 20-40 min, and upregulation by TPA reached maximum levels after 60 min. In summary, our data identify CD13, CD107a, and CD164 as novel basophil-activation antigens. Based on time kinetics of upregulation, we hypothesize that molecules of the “CD203c group” and the “CD63 group” are linked to two different mechanisms of basophil activation. | Florian HENNERSDORF Stefan FLORIAN Andreas JAKOB Katharina BAUMGRTNER Karoline SONNECK Alfred NORDHEIM Tilo BIEDERMANN Peter VALENT Hans-Jrg BüHRING | 2005 | Cell Research2005，15，5： | 9 |
| 3 | Short-term complementary and alternative medicine on quality of life in women with fibromyalgia显示文摘BACKGROUND:Fibromyalgia(FMS)is a syndrome characterized by chronic widespread musculoskeletal pain,whose etiology is not completely understood.Different therapeutic approaches have been used with inconsistent results.This observation does not invalidate the continued search for alternative treatments aimed at improving quality of life(Qo L)in FMS.OBJECTIVE:This study compared three classical traditional Chinese medicine(TCM)therapies:acupuncture(AC),electroacupuncture(EAC)and moxibustion(MX)in the management of pain and promotion of Qo L in FMS patients.DESIGN,SETTING,PARTICIPANTS AND INTERVENTIONS:A preliminary,group-assigned,comparative study enrolled 30 women,mean age(46.90±9.24)years(range 20–60 years),who met the 1990 American College of Rheumatology criteria for FMS diagnosis and a pain-pressure threshold(PPT)< 4 kg/cm^2.The study was conducted in a teaching tertiary-care medical institution from May 2010 through April 2012.AC,EAC and MX were delivered for 30 min,once a week,for 8 weeks,bilaterally at Neiguan(PC6),Hegu(LI4),Yanglingquan(GB34),Sanyinjiao(SP6)and Taichong(LR3)acupoints.MAIN OUTCOME MEASURES:Each week,immediately before treatment and after treatment,subjects were tested for PPTs,Wong-Baker Faces Pain Scale(WBFPS;for pain intensity)and Medical Outcomes Study 36-item Short Form Health Survey(SF-36:for Qo L).RESULTS:There was no signifi cant improvement in pain or reduction of tender points in any of the groups studied,at the end of the 8th session.Signifi cant improvement of Qo L was perceived in vitality(after AC treatment)and in mental health(after EAC and MX treatments).CONCLUSION:TCM therapies(AC,EAC and MX)promoted an improvement in the Qo L in two areas(vitality and mental health)in FMS women.Further large-scale clinical trials are required to confi rm this effect. | Paulo Araujo Dias Andre Brito Bastos Guimaraes Andrea de Oliveira Albuquerque Karoline Lucas de Oliveira Maria Luzete Costa Cavalcante Sergio Botelho Guimaraes | 2016 | Journal of Integrative Medicine2016，14，1： | 7 |
| 4 | 为恶意、良性的胆汁的苛评的治疗的充分盖住的自我可扩充的金属 stents显示文摘 AIM:To present a series of covered self-expandable metal stents(CSEMS) placed for different indications and to evaluate the effectiveness,complications and extractability of these devices.METHODS:We therefore retrospectively reviewed the courses of patients who received CSEMS due to malignant as well as benign biliary strictures and postsphincterotomy bleeding in our endoscopic unit between January 2010 and October 2011.RESULTS:Twenty-six patients received 28 stents due to different indications(20 stents due to malignant biliary strictures,six stents due to benign biliary strictures and two stents due to post-sphincterotomy bleeding).Biliary obstruction was relieved in all cases,regardless of the underlying cause.Hemostasis could be achieved in the two patients who received the stents for this purpose.Complications occurred in five patients(18%).Two patients(7%) developed cholecystitis,stents dislocated/migrated in other two patients(7%),and in one patient(3.6%) stent occlusion was documented during the study period.Seven stents were extracted endoscopically.Removal of stents was easily possible in all cases in which it was desired using standard forceps.Twelve patients underwent surgery with pylorus preserving duodenopancreatectomy.In all patients stents could be removed during the operation without difficulties.CONCLUSION:Despite the higher costs of these devices,fully covered self-expanding metal stents may be suitable to relief biliary obstruction due to bile duct stenosis,regardless of the underlying cause.CSEMS may also represent an effective treatment strategy of severe post-sphincterotomy bleeding,not controlled by other measures. | Ahmed Abdel Samie Stephan Dette Ulrich Vhringer Michael Stumpf Karolin Kopischke Lorenz Theilmann | 2012 | World Journal of Gastrointestinal Endoscopy2012，4，9： | 5 |
| 5 | Eradication of H pylori for the prevention of gastric cancer显示文摘有 H pylori 的感染是与胃的癌症联系的最重要的已知的病因学的因素。当有 H pylori 的胃粘膜的殖民导致活跃、长期的胃炎在时几乎,所有个人感染了,得胃的癌症的可能性取决于环境、细菌的毒力和主人 specific 因素。所有胃的癌症盒子的多数对 H pylori 感染并且因此可归因理论上可防止。从在一个早时间点的 H pylori 的根除能阻止的动物模型有证据胃的癌症开发。然而,使随机化在人在胃的癌症的发生上探索 H pylori 根除的预防效果的临床的审判仍然保持稀少并且让步了冲突结果。为在为 H pylori 的风险的病人的鉴定的更好的标记导致了胃的恶意被需要允许更有效的公共根除策略的发展。同时,屏蔽并且在胃的癌症病人以及某些高风险的人口的一度的亲戚的 H pylori 的治疗可能是有益的。 | Karolin Trautmann Manfred Stolte Stephan Miehlke | 2006 | World Journal of Gastroenterology2006，12，32： | 4 |
| 6 | The measurement of sexual selection on females and males显示文摘 | Karoline FRITZSCHE Isobel BOOKSMYTHE | 2013 | Current Zoology2013，59，4： | 3 |
| 7 | The impact of the EU ETS on the sectoral innovation system for power generation technologies – Findings for Germany显示文摘 | Karoline S. Rogge Volker H. Hoffmann | 2010 | Energy Policy2010，，12： | 1 |
| 8 | Phase I trial of cetuximab in combination with capecitabine, weekly irinotecan, and radiotherapy as neoadjuvant therapy for rectal cancer显示文摘 | Ralf-Dieter Hofheinz Karoline Horisberger Christoph Woernle Frederik Wenz Uta Kraus-Tiefenbacher Georg K?hler Dietmar Dinter Rainer Grobholz Steffen Heeger Stefan Post Andreas Hochhaus Frank Willeke | 2006 | International Journal of Radiation Oncology Biology Physics2006，，5： | 1 |
| 9 | Identification and characterization of Lbh, a novel conserved nuclear protein expressed during early limb and heart development显示文摘 | KAROLINE J B ALEXANDRA L J | 2001 | Developmental Biology2001，233，： | 1 |
| 10 | Contributors to individual quality of life after liver transplantation显示文摘 | Christian Thiel Kerstin Landgrebe Evelyn Knubben Silvio Nadalin Ruth Ladurner Christian Grasshoff Alfred K?nigsrainer Martin Schenk Karolin Thiel | 2012 | European Journal of Clinical Investigation2012，，1： | 1 |
| 11 | Fluorescence and Absorption Spectroscopic Properties of Dipyrrometheneboron Difluoride(BODlPY) Derivatives in Liquids,Lipid Membranes,and Proteins显示文摘 | Karolin J Johansson B A Strundberg L | 1994 | J Am Chem Soc1994，116，： | 1 |
| 12 | 18FDG PET in oncology: The best and the worst (Review)显示文摘 | Tarik Belhocine Karoline Spaepen Michelle Dusart Catherine Castaigne Kristof Muylle Pierre Bourgeois Daniel Bourgeois Lawrence Dierickx Patrick Flamen | 2006 | International Journal of Oncology2006，，5： | 1 |
| 13 | Effect of solvent content on the separation and the energy consumption of extractive distillation columns显示文摘 | De Figueiredo M F Brito Karoline Dantas Ramos W B | 2015 | Chemical Engineering Communi- cations2015，202，9： | 1 |
| 14 | Porcine model characterizing various parameters assessing the outcome after acetaminophen intoxication induced acute liver failure显示文摘AIM To investigate the changes of hemodynamic and laboratory parameters during the course of acute liver failure following acetaminophen overdose.METHODS Eight pigs underwent a midline laparotomy following jejunal catheter placement for further acetaminophen intoxication and positioning of a portal vein Doppler flow-probe. Acute liver failure was realized by intrajejunal acetaminophen administration in six animals, two animals were sham operated. All animals were invasively monitored and received standardized intensive care support throughout the study. Portal blood flow, hemodynamic and ventilation parameters were continuously recorded. Laboratory parameters were analysed every eight hours. Liver biopsies were sampled every 24 h following intoxication and upon autopsy.RESULTS Acute liver failure (ALF) occurred after 28 ± 5 h resulted in multiple organ failure and death despite maximal support after further 21 ± 1 h (study end). Portal blood flow (baseline 1100 ± 156 m L/min) increased to a maximum flow of 1873 ± 175 m L/min at manifestation of ALF, which was significantly elevated(P < 0.01). Immediately after peaking, portal flow declined rapidly to 283 ± 135 m L/min at study end. Thrombocyte values (baseline 307 × 103/μL± 34 × 103/μL) of intoxicated animals declined slowly to values of 145 × 103/μL± 46 × 103/μL when liver failure occurred. Subsequent appearance of severe thrombocytopenia in liver failure resulted in values of 11 × 103/μL± 3 × 103/μL preceding fatality within few hours which was significant(P > 0.01).CONCLUSION Declining portal blood flow and subsequent severe thrombocytopenia after acetaminophen intoxication precede fatality in a porcine acute liver failure model. | Karolin Thiel Wilfried Klingert Kathrin Klingert Matthias H Morgalla Martin U Schuhmann Pamela Leckie Yalda Sharifi Nathan A Davies Rajiv Jalan Andreas Peter Christian Grasshoff Alfred Konigsrainer Martin Schenk Christian Thiel | 2017 | World Journal of Gastroenterology2017，23，9： | 1 |
| 15 | Detection of molecular targets on the surface of CD34+/CD38? stem cells in various myeloid malignancies显示文摘 | Stefan Florian Karoline Sonneck Alexander W. Hauswirth Maria-Theresa Krauth Gerit-Holger Schernthaner Wolfgang R. Sperr Peter Valent | 2006 | Leukemia & Lymphoma2006，，2： | 1 |
| 16 | Host-derived angiopoietin-2 affects early stages of tumor development and vessel maturation but Is dispensable for later stages of tumor growth显示文摘 | Patrick N Markus T Karoline K | | 0，，： | 1 |
| 17 | Chloride sensitive probes for biological applications显示文摘 | Apperson K Karolin J | 2001 | Dye & Pigments2001，48，： | 1 |
| 18 | Endothelial Dysfunction, Oxidative Stress, and Risk of Cardiovascular Events in Patients With Coronary Artery Disease显示文摘 | Thomas Heitzer Titus Schlinzig Karoline Krohn Thomas Meinertz Thomas Münzel | 2001 | Circulation: Journal of the American Heart Association2001，，22： | 1 |
| 19 | EU emissions trading: an early analysis of national allocation plans for 2008–2012显示文摘 | Regina Betz Karoline Rogge Joachim Schleich | 2006 | Climate Policy2006，，： | 1 |
| 20 | Effects of reserve pool on the relationship between respiration and photosynthesis显示文摘 | MOLDAU H KAROLIN A | 1977 | Photosynthetica1977，11，： | 1 |
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