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| 1 | Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma显示文摘Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer featured with high intra-tumoral heterogeneity and poor prognosis. To comprehensively delineate the PDAC intra-tumoral heterogeneity and the underlying mechanism for PDAC progression, we employed single-cell RNA-seq (scRNA-seq) to acquire the transcriptomic atlas of 57,530 individual pancreatic cells from primary PDAC tumors and control pancreases, and identified diverse malignant and stromal cell types, including two ductal subtypes with abnormal and malignant gene expression profiles respectively, in PDAC. We found that the heterogenous malignant subtype was composed of several subpopulations with differential proliferative and migratory potentials. Cell trajectory analysis revealed that components of multiple tumor-related pathways and transcription factors (TFs) were differentially expressed along PDAC progression. Furthermore, we found a subset of ductal cells with unique proliferative features were associated with an inactivation state in tumor-infiltrating T cells, providing novel markers for the prediction of antitumor immune response. Together, our findings provide a valuable resource for deciphering the intra-tumoral heterogeneity in PDAC and uncover a connection between tumor intrinsic transcriptional state and T cell activation, suggesting potential biomarkers for anticancer treatment such as targeted therapy and immunotherapy. | Junya Peng Bao-Fa Sun Chuan-Yuan Chen Jia-Yi Zhou Yu-Sheng Chen Hao Chen Lulu Liu Dan Huang Jialin Jiang Guan-Shen Cui Ying Yang Wenze Wang Dan Guo Menghua Dai Junchao Guo Taiping Zhang Quan Liao Yi Liu Yong-Liang Zhao Da-Li Han Yupei Zhao Yun-Gui Yang Wenming Wu | 2019 | Cell Research2019，29，9： | 24 |
| 2 | Development and validation of an endoscopic images-based deep learning model for detection with nasopharyngeal malignancies显示文摘Background:Due to the occult anatomic location of the nasopharynx and frequent presence of adenoid hyperpla-sia,the positive rate for malignancy identification during biopsy is low,thus leading to delayed or missed diagnosis for nasopharyngeal malignancies upon initial attempt.Here,we aimed to develop an artificial intelligence tool to detect nasopharyngeal malignancies under endoscopic examination based on deep learning.Methods:An endoscopic images-based nasopharyngeal malignancy detection model(eNPM-DM)consisting of a fully convolutional network based on the inception architecture was developed and fine-tuned using separate training and validation sets for both classification and segmentation.Briefly,a total of 28,966 qualified images were collected.Among these images,27,536 biopsy-proven images from 7951 individuals obtained from January 1st,2008,to December 31st,2016,were split into the training,validation and test sets at a ratio of 7:1:2 using simple randomiza-tion.Additionally,1430 images obtained from January 1st,2017,to March 31st,2017,were used as a prospective test set to compare the performance of the established model against oncologist evaluation.The dice similarity coef-ficient(DSC)was used to evaluate the efficiency of eNPM-DM in automatic segmentation of malignant area from the background of nasopharyngeal endoscopic images,by comparing automatic segmentation with manual segmenta-tion performed by the experts.Results:All images were histopathologically confirmed,and included 5713(19.7%)normal control,19,107(66.0%)nasopharyngeal carcinoma(NPC),335(1.2%)NPC and 3811(13.2%)benign diseases.The eNPM-DM attained an overall accuracy of 88.7%(95%confidence interval(CI)87.8%-89.5%)in detecting malignancies in the test set.In the prospective comparison phase,eNPM-DM outperformed the experts:the overall accuracy was 88.0%(95%CI 86.1%-89.6%)vs.80.5%(95%CI 77.0%-84.0%).The eNPM-DM required less time(40 s vs.110.0±5.8 min)and exhibited encouraging performance in automatic segmentation of nasopharyngeal malignant area from the background,with an average DSC of 0.78±0.24 and 0.75±0.26 in the test and prospective test sets,respectively.Conclusions:The eNPM-DM outperformed oncologist evaluation in diagnostic classification of nasopharyngeal mass into benign versus malignant,and realized automatic segmentation of malignant area from the background of nasopharyngeal endoscopic images. | Chaofeng Li Bingzhong Jing Liangru Ke Bin Li Weixiong Xia Caisheng He Chaonan Qian Chong Zhao Haiqiang Mai Mingyuan Chen Kajia Cao Haoyuan Mo Ling Guo Qiuyan Chen Linquan Tang Wenze Qiu Yahui Yu Hu Liang Xinjun Huang Guoying Liu Wangzhong Li Lin Wang Rui Sun Xiong Zou Shanshan Guo Peiyu Huang Donghua Luo Fang Qiu Yishan Wu Yijun Hua Kuiyuan Liu Shuhui Lv Jingjing Miao Yanqun Xiang Ying Sun Xiang Guo Xing Lv | 2018 | Cancer Communications2018，38，1： | 9 |
| 3 | Improvement of Urban Impervious Surface Estimation in Shanghai Using Landsat7 ETM+ Data显示文摘This paper explores the potential to improve the impervious surface estimation accuracy using a multi-stage approach on the basis of vegetation-impervious surface-soil (V-I-S) model. In the first stage of Spectral Mixture Analysis (SMA) process, pixel purity index, a quantitative index for defining endmember quality, and a 3-dimensional endmember selection method were applied to refining endmembers. In the second stage, instead of obtaining impervious surface fraction by adding high and low albedo fractions directly, a linear regression model was built between impervious surface and high/low albedo using a random sampling method. The urban impervious surface distribution in the urban central area of Shanghai was predicted by the linear regression model. Estimation accuracy of spectral mixture analysis and impervious surface fraction were assessed using root mean square (RMS) and color aerial photography respectively. In comparison with three different research methods, this improved estimation method has a higher overall accuracy than traditional Linear Spectral Mixture Analysis (LSMA) method and the normalized SMA model both in root mean square error (RMSE) and standard error (SE). However, the model has a tendency to overestimate the impervious surface distribution. | YUE Wenze | 2009 | Chinese Geographical Science2009，19，3： | 7 |
| 4 | Storage and Density of Soil Organic Carbon in Urban Topsoil of Hilly Cities:A Case Study of Chongqing Municipality of China显示文摘Rapid urbanization results in the conversion of natural soil to urban soil,and consequently,the storage and density of the soil carbon pools change.Taking Chongqing Municipality of China as a study case,this investigation attempts to better understand soil carbon pools in hilly cities.First,the vegetated areas in the study area were derived from QuickBird images.Then,topsoil data from 220 soil samples(0-20 cm) in the vegetated areas were collected and their soil organic carbon(SOC) densities were analyzed.Using the Kriging interpolation method,the spatial pattern of SOC was estimated.The results show that the SOC density exhibited high spatial variability in the urban topsoil of Chongqing.First,the SOC density in topsoil decreased according to slope in the order 2°-6° < 25°-90° < 0°-2° < 6°-15° < 15°-25°.Second,the newly developed areas during 2001-2010 had a lower SOC density than the areas built before 1988.Third,urban parks and gardens had a higher SOC density in topsoil,residential green land followed,and scattered street green land ranked last.For hilly cities,the variability of terrain affects the distribution of SOC.The Kriging results indicate that Kriging method combining slope with SOC density produced a high level of accuracy.The Kriging results show that the SOC density to the north of the Jialing River was higher than the south.The vegetated areas were estimated to amount to 73.5 km2 across the study area with an SOC storage of 0.192 Tg and an average density of 2.61 kg/m2. | LIU Yong WANG Cheng YUE Wenze HU Yanyan | 2013 | Chinese Geographical Science2013，23，1： | 6 |
| 5 | Predicting dynamic cellular protein–RNA interactions by deep learning using in vivo RNA structures显示文摘Interactions with RNA-binding proteins(RBPs)are integral to RNA function and cellular regulation,and dynamically reflect specific cellular conditions.However,presently available tools for predicting RBP–RNA interactions employ RNA sequence and/or predicted RNA structures,and therefore do not capture their condition-dependent nature.Here,after profiling transcriptome-wide in vivo RNA secondary structures in seven cell types,we developed PrismNet,a deep learning tool that integrates experimental in vivo RNA structure data and RBP binding data for matched cells to accurately predict dynamic RBP binding in various cellular conditions.PrismNet results for 168 RBPs support its utility for both understanding CLIP-seq results and largely extending such interaction data to accurately analyze additional cell types.Further,PrismNet employs an“attention”strategy to computationally identify exact RBP-binding nucleotides,and we discovered enrichment among dynamic RBP-binding sites for structure-changing variants(riboSNitches),which can link genetic diseases with dysregulated RBP bindings.Our rich profiling data and deep learning-based prediction tool provide access to a previously inaccessible layer of cell-type-specific RBP–RNA interactions,with clear utility for understanding and treating human diseases. | Lei Sun Kui Xu Wenze Huang Yucheng T.Yang Pan Li Lei Tang Tuanlin Xiong Qiangfeng Cliff Zhang | 2021 | Cell Research2021，31，5： | 6 |
| 6 | Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2显示文摘In contrast to the extensive research about viral protein–host protein interactions that has revealed major insights about how RNA viruses engage with host cells during infection,few studies have examined interactions between host factors and viral RNAs(vRNAs).Here,we profiled vRNA–host protein interactomes for three RNA virus pathogens(SARS-CoV-2,Zika,and Ebola viruses)using ChIRP-MS.Comparative interactome analyses discovered both common and virus-specific host responses and vRNA-associated proteins that variously promote or restrict viral infection.In particular,SARS-CoV-2 binds and hijacks the host factor IGF2BP1 to stabilize vRNA and augment viral translation.Our interactome-informed drug repurposing efforts identified several FDA-approved drugs(e.g.,Cepharanthine)as broad-spectrum antivirals in cells and hACE2 transgenic mice.A co-treatment comprising Cepharanthine and Trifluoperazine was highly potent against the newly emerged SARS-CoV-2 B.1.351 variant.Thus,our study illustrates the scientific and medical discovery utility of adopting a comparative vRNA-host protein interactome perspective. | Shaojun Zhang Wenze Huang Lili Ren Xiaohui Ju Mingli Gong Jian Rao Lei Sun Pan Li Qiang Ding Jianwei Wang Qiangfeng Cliff Zhang | 2022 | Cell Research2022，32，1： | 5 |
| 7 | Impact of economic development on wetlands in Hangzhou Bay Industrial Belt显示文摘把 TM 基于 Landsat 在 2005 的图象和在 Hangzhou 海湾工业带的 20052020 的城市的计划数据,这份报纸由雇用 GIS 学习了对沼泽地的计划城市的构造陆地扩展的潜在的风险空间分析方法。结果显示出那:(1 ) 沼泽地资源广泛地分布式、多样,与空间地介绍城市的构造陆地的近似分布。(2 ) 从 2005 ~ 2020,城市的构造登陆将极大地并且很快膨胀,并且将在 Hangzhou 海湾南方海岸的开垦区域接管主要是池溏的沼泽地的大区域和水产养殖水区域。而且,这个变化将显然是空间地不平的,例如,沼泽地的最严肃的衰退在 Ningbo 城市, Hangzhou 城市和 Cixi 城市里被集中。(3 ) 总的来说,到沼泽地的潜在的风险很高,并且将不停地与都市化的趋势增加。(4 ) 数量和计划的空间模式城市的构造土地在沼泽地损失上有实质的影响。因此,一些政策和规定被建议土地计划协调在象调整构造那样的城市的经济开发和沼泽地资源保护之间的关系,补偿象构造沼泽地公园一样的沼泽地职业。 | REN Liyan YUE Wenze LI Jialin WU Cifang | 2010 | Journal of Geographical Sciences2010，20，3： | 4 |
| 8 | Remote sensing of spatial patterns of urban renewal using linear spectral mixture analysis: A case of central urban area of Shanghai (1997-2000)显示文摘It is very important to integrate remote sensing with urban geography that the spectral mix- ture analysis technique is applied to urban land cover evolvement and its eco-environmental effect. Urban land cover is mainly composed of complicated artifi- cial materials, which is the key factor to limit the de- velopment of the spectral mixture analysis technique. There are two main aspects in which the technique of spectral mixture analysis is applied to urban geog- raphy: one is to calculate vegetation fraction; the other is to build quantitative model of the urban im- pervious surface obtained from the combination be- tween high albedo fraction and low albedo fraction. The technique of spectral mixture analysis is firstly applied to study urban renewal pattern, scale and mode which happened in Shanghai City from 1997 to 2000. | YUE Wenze XU Jianhua WU Jiawei XU Lihua | 2006 | Chinese Science Bulletin2006，51，8： | 4 |
| 9 | Development of Perennial Wheat Through Hybridization Between Wheat and Wheatgrasses: A Review显示文摘 | Lei Cui Yongkang Ren Timothy D. Murray Wenze Yan Qing Guo Yuqi Niu Yu Sun Hongjie Li | 2018 | Engineering2018，4，4： | 4 |
| 10 | Prognostic value of MET, cyclin D1 and MET gene copy number in non-small cell lung cancer显示文摘The aim of this study was to analyze the correlation of the expression of MET and cyclin D1 and MET gene copy number in non-small cell lung cancer (NSCLC) tissues and patient clinicopathologic characteristics and survival. Sixty-one NSCLC tissue specimens were included in the study. The expression of MET and cyclin D1 was evaluated by immunohistochemistry and MET gene copy number was assessed by quantitative real-time polymerase chain reaction (Q-PCR). Positive expression of MET and cyclin D1 protein and increased MET gene copy number occurred in 59.0%, 59.0% and 18.0% of 61 NSCLC tissues, respectively. MET-positivity correlated with poor differentiation (P=0.009). Increased MET gene copy number was significantly associated with lymph node metastasis (P=0.004) and advanced tumor stage (P=0.048), while the expression of cyclin D1 was not associated with any clinicopathologic parameters. There was a significant correlation between the expression of MET and MET gene copy number (P=0.002). Additionally, the expression of cyclin D1 had a significant association with the expression of MET as well as MET gene copy number (P=0.002 and P=0.017, respectively). MET-positivity and increased MET gene copy number were significantly associated with poor overall survival (P=0.003 and P<0.001, respectively) in univariate analysis. Multivariate Cox proportional hazard analysis confirmed that the expression of MET and MET gene copy number were prognostic indicators of NSCLC (P=0.003 and P=0.001, respectively). The overexpression of MET and the increased MET gene copy number might be adverse prognostic factors for NSCLC patients. The activation of the MET/cyclin D1 signaling pathway may contribute to carcino- genesis and the development of NSCLC, and may represent a target for therapy. | Wenze Sun Liping Song Ting Ai Yingbing Zhang Ying Gao Jie Cui | 2013 | The Journal of Biomedical Research2013，27，3： | 3 |
| 11 | A preliminary report of the Yangbi, Yunnan, M_(S)6.4 earthquake of May 21, 2021显示文摘According to the reports of China Earthquake Networks Center,an Ms6.4 earthquake occurred in Yangbi City,Dali Prefecture,Yun-nan Province,on May 21,2021;the epicenter was located at 25.67°N and 99.87°E with a focal depth of 8 km.Within 5 km from the epicenter the average elevation is 2268 m. | ZhiGao Yang Jie Liu Xue-Mei Zhang WenZe Deng GuangBao Du XiYan Wu | 2021 | Earth and Planetary Physics2021，5，4： | 3 |
| 12 | Partial discharge measurement and analysis under lightning impulse superimposed DC voltage in SF6 gas显示文摘The presence of lightning impulses(LIs)on the DC waveform can seriously influence the insulation performance of a GIL system.The waveshape can be a superposition of the DC and impulse waveform.In this study,the behaviour of partial discharge(PD)on an epoxy surface is investigated under DC and LI superimposed voltage in SF6.The lightning impulses with different polarities are superimposed on the epoxy surface under both positive and negative DC voltages,respectively.Positive lightning impulses are superimposed on a negative DC voltage with a 90%of the partial discharge inception voltage(PDIV)magnitude.The number and amplitude of the PDs are used to evaluate the insulation performance.The experimental results show that the LI with a different polarity applied to DC can excite the PDs on the epoxy surface.The excitation effect of positive LI superimposed on negative DC is more significant.According to the impulse amplitude and PD characteristics,the influence of superimposed LI on PD excitation can be divided into three stages.As the superimposed LI increases,the amplitude of the excited PD increases gradually,while the PD number first increases and then decreases.This study is expected to provide reference for manufacture and protection of power equipment. | Haotian Wang Cong He Ruochen Guo Xuan Meng Wenze Yuan Junhao Li | 2022 | High Voltage2022，7，4： | 3 |
| 13 | Ultrastructural findings after intraarticular application of hyaluronan in a canine model of arthropathy显示文摘 | Wenz W Breusch SJ Graf J | | Journal of Orthopaedic Research0，，： | 2 |
| 14 | Enhancing effect of phase circular statistics vector for improvement imaging quality of ultrasonic total focusing method显示文摘To further improve the quality of the total focusing method(TFM)imaging,an ultrasonic TFM based on phase circular statistics vector(PCSV)has been presented.Firstly,the information of the signal phases of the full matrix capture(FMC)aperture data was extracted in the TFM implement processes.The phases of the same focusing pixel were regarded as the samples of the circular statistics.Then the PCSVs determined by the signal amplitudes and phases were acquired using the operation of saturation weighted statistics.Finally,the magnitudes of the PCSV were equivalent to the amplitudes of the TFM image.The results showed the TFM-PCSV was capable of improving SNR by amplifying the contribution of phase information.Also,the method could improve the lateral resolution by enhancing aperture beam directivity.Compared to the phase coherence weighted algorithm SCF and CCF,the TFM-PCSV had a higher tolerance to the error of phases. | MAO Qiuqin CHEN Yao ZHANG Baiyuan SHI Wenze LU Chao LI Qiufeng | 2021 | Chinese Journal of Acoustics2021，40，2： | 2 |
| 15 | An Introductory Overview of Earthquake Early Warning显示文摘Earthquake early warning(EEW)is discriminated from earthquake prediction by using initial seismic waves to predict the severity of ground motion and issue the warning information to potential affected area.The warning information is useful to mitigate the disaster and decrease the losses of life and economy.We reviewed the development history of EEW worldwide and summarized the methodologies using in different systems.Some new sensors came and are coming into EEW giving more developing potential to future implementation.The success of earthquake disaster mitigation relies on the cooperation of the whole society. | SUN Li DENG Wenze DAI Danqing | 2019 | Earthquake Research in China2019，33，4： | 2 |
| 16 | Mini-incision total hip arthroplesty: a comparative assessment of perioperative outcomes 显示文摘 | Wenz James F Gurkan llksen Jibodh Stefan R | 2002 | Orthopedics2002，25，10： | 2 |
| 17 | Adjuvant IMRT/XELOX radiochemotherapy improves long-term overall- and disease-free survival in advanced gastric cancer显示文摘 | J. Boda-Heggemann C. Weiss V. Schneider R.-D. Hofheinz S. Haneder H. Michaely H. Wertz U. Ronellenfitsch A. Hochhaus F. Wenz F. Lohr | 2013 | Strahlentherapie und Onkologie2013，，5： | 2 |
| 18 | Drosophila ubiquitin E3 ligase dSmurf is required for synapse remodeling and axon pruning by glia显示文摘Animal behaviors and higher-order functions rely on complex neural circuits built by synaptic connections(synapses)to deliver messages among different brain cells.As the major mediator in the nervous system,neurons communicate via synapses,which undergo constant structural remodeling with strict regulation.It | Changyan Chen Shuai Yin Wenze Cao Margaret S. Ho | 2017 | Journal of Genetics and Genomics2017，44，1： | 2 |
| 19 | Medical prevention and treatmerit of acute and chronic radiation induced enteritis is there any proven therapy显示文摘 | Zimmerer T Btieker U Wenz F | 2008 | Gastroenteml2008，46，5： | 1 |
| 20 | The activity of γδ T cells against paediatric liver tumour cells and spheroids in cell culture显示文摘 | Alexander Hoh Alexander Dewerth Fabian Vogt Julia Wenz Patrick A. Baeuerle Steven W. Warmann Joerg Fuchs Sorin Armeanu‐Ebinger | 2012 | Liver Int2012，，1： | 1 |
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