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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Human acute myelogenous leukemia stem cells are rare and heterogeneous when assayed in NOD/SCID/IL2R[gamma]c-deficient mice显示文摘 | Sarry Jean-Emmanuel Murphy Kathleen Perry Robin Sanchez Patricia V Secreto Anthony Keefer Cathy Swider Cezary R Strzelecki Anne-Claire Cavelier Cindy Récher Christian Mansat-De Mas Véronique Delabesse Eric Danet-Desnoyers G Carroll Martin | 2011 | Journal of Clinical Investigation2011，，1： | 2 |
| 3 | Global prevalence of dementia: a Delphi consensus study显示文摘 | Cleusa P Ferri Martin Prince Carol Brayne Henry Brodaty Laura Fratiglioni Mary Ganguli Kathleen Hall Kazuo Hasegawa Hugh Hendrie Yueqin Huang Anthony Jorm Colin Mathers Paulo R Menezes Elizabeth Rimmer Marcia Scazufca | 2005 | The Lancet2005，，9503： | 2 |
| 4 | Global prevalence of dementia: a Delphi consensus study显示文摘 | Cleusa P Ferri Martin Prince Carol Brayne Henry Brodaty Laura Fratiglioni Mary Ganguli Kathleen Hall Kazuo Hasegawa Hugh Hendrie Yueqin Huang Anthony Jorm Colin Mathers Paulo R Menezes Elizabeth Rimmer Marcia Scazufca | 2005 | The Lancet . 2005 (9503)2005，，9503： | 2 |
| 5 | Bringing Engineer- ing Research to Market: How Universities, Industry and Government Are Attempting to Solve the Problem显示文摘 | Allen Kathleen R Taylor Cyrus C | 2005 | Engineering Management Journal2005，17，3： | 1 |
| 6 | General characteristics of temperature variation in China during the last two millennia显示文摘 | Ban Yang Achim Braeuning Kathleen R Johnson | 2002 | Geophysical Research Letters2002，29，9： | 1 |
| 7 | A Historical Comparison of Resource-based Theory and Five SchooIs of Thought Within Industrial Organization Economics: Do We Have a New Theory of the Firm? 显示文摘 | Conner Kathleen R | 1991 | Journal of Management1991，17，1： | 1 |
| 8 | Designing eco-industrial water exchange networks using fuzzy mathematical programming显示文摘 | KATHLEEN B RAYMOND R ALVIN B | 2009 | 8: 9548-95582009，8，： | 1 |
| 9 | prospective proof of concept study of the efficacy of tacrolimus ointment onuraemicpruritus (UP) in patients on chronic dialysis therapy显示文摘 | Dirk R Kuypers Kathleen Claes Pieter Eveneplel | 2004 | Nephrol Dial Transplant2004，19，： | 1 |
| 10 | Lung cancer screening with CT显示文摘 | Aberle D R Kathleen B | 2008 | Clin Chest Med2008，29，： | 1 |
| 11 | Nutrient additions generate prolific growth of Lyngbya majuscule (cyanobacteria) in field and bioassay experiments显示文摘 | KATHLEEN S A COLIN R A JAMES W U | 2007 | Harmful Algae2007，6，： | 1 |
| 12 | 显示文摘 | Scott R S Ken B A Kathleen A C | 2002 | Applied Catalysis2002，231，： | 1 |
| 13 | In situ field experiment shows Lyngbya majuscula (cyanobacterium) growth stimulated by added iron,phosphorus and nitrogen显示文摘 | KATHLEEN S A COLIN R A JAMES W U | 2008 | Harmful Algae2008，7，： | 1 |
| 14 | The Notch signaling pathway as a mediator of tumor survival 显示文摘 | Kathleen M Capaccione Sharon R P | 2013 | Carcinogenesis2013，34，7： | 1 |
| 15 | Capsaicin displays anti-proliferative activity against human small cell lung cancer in cell culture and nude mice models via the E2F pathway显示文摘 | KATHLEEN C BROWN TED R | 2010 | PLoS One2010，5，10： | 1 |
| 16 | Comparison of two dif- ferent methods for inoculating VX2 tumors in rabbit livers and hind limbs显示文摘 | Virmani S Kathleen R Harris BS | 2008 | J Vase Interv Radiol2008，19，6： | 1 |
| 17 | 显示文摘 | Scott R S Ken B A Kathleen A C | 2002 | Applied Catalysis2002，231，： | 1 |
| 18 | The toxicity of microcystin-LR in mice following 7 days of inhalation exposure显示文摘 | JANET M B JULIE A H KATHLEEN R | 2005 | Toxicon2005，45，： | 1 |
| 19 | Internet Use for Health Information among College Student 显示文摘 | Cam Escoffery Kathleen R Miner | 2005 | Journal of American College Health2005，53，4： | 1 |
| 20 | Antimicrobial residues in animal waste and water resources proximal to large-scale swine and poultry feeding operations显示文摘 | Enzo R Campagnolo Kammy R Johnson Adam Karpati Carol S Rubin Dana W Kolpin Michael T Meyer J.Emilio Esteban Russell W Currier Kathleen Smith Kendall M Thu Michael McGeehin | 2002 | Science of the Total Environment2002，，1： | 1 |
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