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| 1 | Engineering of Yarrowia lipolytica for production of astaxanthin显示文摘Astaxanthin is a red-colored carotenoid,used as food and feed additive.Astaxanthin is mainly produced by chemical synthesis,however,the process is expensive and synthetic astaxanthin is not approved for human consumption.In this study,we engineered the oleaginous yeast Yarrowia lipolytica for de novo production of astaxanthin by fermentation.First,we screened 12 different Y.lipolytica isolates for β-carotene production by introducing two genes for β-carotene biosynthesis:bi-functional phytoene synthase/lycopene cyclase(crtYB)and phytoene desaturase(crtI)from the red yeast Xanthophyllomyces dendrorhous.The best strain produced 31.1±0.5 mg/L β-carotene.Next,we optimized the activities of 3-hydroxy-3-methylglutaryl-coenzyme A reductase(HMG1)and geranylgeranyl diphosphate synthase(GGS1/crtE)in the best producing strain and obtained 453.9±20.2 mg/L β-carotene.Additional downregulation of the competing squalene synthase SQS1 increased the β-carotene titer to 797.1±57.2 mg/L.Then we introduced β-carotene ketolase(crtW)from Paracoccus sp.N81106 and hydroxylase(crtZ)from Pantoea ananatis to convert β-carotene into astaxanthin.The constructed strain accumulated 10.4±0.5 mg/L of astaxanthin but also accumulated astaxanthin biosynthesis intermediates,5.7±0.5 mg/L canthaxanthin,and 35.3±1.8 mg/L echinenone.Finally,we optimized the copy numbers of crtZ and crtW to obtain 3.5 mg/g DCW(54.6 mg/L)of astaxanthin in a microtiter plate cultivation.Our study for the first time reports engineering of Y.lipolytica for the production of astaxanthin.The high astaxanthin content and titer obtained even in a small-scale cultivation demonstrates a strong potential for Y.lipolytica-based fermentation process for astaxanthin production. | Kanchana Rueksomtawin Kildegaard Belen Adiego-Perez David Domenech Belda Jaspreet Kaur Khangura Carina Holkenbrink Irina Borodina | 2017 | Synthetic and Systems Biotechnology2017，2，4： | 12 |
| 2 | Thermal decomposition and kinetics of plastic bonded explosives based on mixture of HMX and TATB with polymer matrices显示文摘This work describes thermal decomposition behaviour of plastic bonded explosives(PBXs) based on mixture of 1,3,5,7-tetranitro-1,3,5,7-tetrazocane(HMX) and 2,4,6-triamino-1,3,5-trinitrobenzene(TATB)with Viton A as polymer binder. Thermal decomposition of PBXs was undertaken by applying simultaneous thermal analysis(STA) and differential scanning calorimetry(DSC) to investigate influence of the HMX amount on thermal behavior and its kinetics. Thermogravimetric analysis(TGA) indicated that the thermal decomposition of PBXs based on mixture of HMX and TATB was occurred in a three-steps. The first step was mainly due to decomposition of HMX. The second step was ascribed due to decomposition of TATB, while the third step was occurred due to decomposition of the polymer matrices. The thermal decomposition % was increased with increasing HMX amount. The kinetics related to thermal decomposition were investigated under non-isothermal for a single heating rate measurement. The variation in the activation energy of PBXs based on mixture of HMX and TATB was observed with varying the HMX amount. The kinetics from the results of TGA data at various heating rates under non-isothermal conditions were also calculated by Flynn—Wall—Ozawa(FWO) and Kissinger-Akahira-Sunose(KAS)methods. The activation energies calculated by employing FWO method were very close to those obtained by KAS method. The mean activation energy calculated by FWO and KAS methods was also a good agreement with the activation energy obtained from single heating rate measurement in the first step decomposition. | Arjun Singh Tirupati C.Sharma Mahesh Kumar Jaspreet Kaur Narang Prateek Kishore Alok Srivastava | 2017 | Defence Technology(防务技术)2017，13，1： | 8 |
| 3 | Risk of complications and urinary incontinence following cytoreductive prostatectomy: a multi-institutional study显示文摘当时,新兴的证据建议了 cytoreductive 前列腺切除术(CRP ) 允许优异 oncologic 控制与照顾雄激素剥夺标准的电流相比治疗独自一个。然而,在变形前列腺癌症(mPCa ) 的 cytoreduction 的安全和利益没被证明。因此,我们评估了在最新与 mPCa 诊断的人跟随 CRP 的复杂并发症的发生。在四个第三级的外科的中心从 2006 ~ 2014 经历了 CRP 的 68 个病人的一个总数与为临床上局部性的前列腺癌症(PCa ) 经历了激进的前列腺切除术的 598 个人相比。尿不能自制被定义为任何垫的使用。CRP 有更长起作用的时间(200 min 对 140 min, P < 0.0001 ) 并且更高估计的血损失(250 ml 对 125 ml, P < 0.0001 ) 与控制组相比。然而,外套(8.82% 对 5.85%) 和主要复杂并发症率(4.41% 对 2.17%) 在二个组之间是可比较的。重要地,尿不能自制率在在外科以后 1 年在 CRP 组是显著地更高的(57.4% 对 90.8% , P < 0.0001 ) 。Univariate 逻辑分析证明估计的血损失在 unadjusted 模型两个都是 perioperative 复杂并发症的唯一的独立预言者(或:1.18;95% CI:1.02-1.37;P = 0.025 ) 并且外科调整类型的模型(或:1.17;95% CI:1.01-1.36;P = 0.034 ) 。在结论, CRP 比激进的前列腺切除术更挑战性、与尿不能自制的尤其是更高的发生联系。不过, CRP 是为选择谁的 PCa 病人的一个技术上可行、安全的手术与节点积极或多骨的转移在场当由富有经验的外科医生表现了时。未来的、多机构的临床的试用当前是在进行之中的验证这个概念。 | Dae Keun Kim Jaspreet Singh Parihar Young Suk Kwon Sinae Kim Brian Shinder Nara Lee Nicholas Farber Thomas Ahlering Douglas Skarecky Bertram Yuh Nora Ruel Wun-lae Kim Koon Ho Rha Isaac Yi Kim | 2018 | Asian Journal of Andrology2018，20，1： | 5 |
| 4 | In vitro evaluation of antimicrobial and antioxidant activities of methanolic extract of Jasminum humile Leaves显示文摘Objective:To evaluate in vitro antimicrobial and antioxidant activities of methanolic extract of Jasminum humile(J.humile) leaves extract.Method:Methanolic extract of J.humile was evaluated for its antimicrobial activity by using agar well diffusion method & their possible antioxidant assay by two complementary test systems,namely DPPH and hydrogen peroxide scavenging activity.These various antioxidant activities were compared to standard antioxidants such as ascorbic acid for both the tests.Results:In the DPPH & hydrogen peroxide scavenging activity,the IC50 value of methanol extract was 70.43μg/mL & 60.79μg/mL respectively. Further,the extract showed inhibitory activity for Gram-positive and negative bacteria at different concentrations.The maximum antibacterial activity of extract was exhibited against Staphylococcus aureus(S.aureus) at concentration 50 mg/mL when compared with ciprofloxacin Conclusions:These results clearly indicate that J.humile is effective in scavenging free radicals and has the potential to be a powerful antioxidant.Thus,the results obtained in the present study indicate that J.humile leaves extract could be considered as a potential source of natural antioxidants and that could be used as an effective source against bacterial diseases. | Parminder Nain Ashok Kumar Sunil Sharma Jaspreet Nain | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，10： | 3 |
| 5 | Mechanism of cell death during warm hepatic ischemia-reperfusion in rats: Apoptosis or necrosis?显示文摘 | Jaspreet S. Gujral Thomas J. Bucci Anwar Farhood Hartmut Jaeschke | 2001 | Hepatology2001，，2： | 2 |
| 6 | Effects of different ingredients and microwave power on popping characteristics of popcorn 显示文摘 | Jaspreet S Narpinder S | 1999 | Journal of Food Engineering1999，，42： | 1 |
| 7 | Factors influencing the physico-chemical, morphological, thermal and rheological properties of some chemically modified starches for food applications-a review 显示文摘 | Jaspreet Singh Lovedeep Kaur McCarthy O J | 2007 | Food Hydrocolloids2007，21，： | 1 |
| 8 | Experimental study on transport mechanisms during deep fat frying of chicken nuggets 显示文摘 | Sravan L Jaspreet S S Pawan S T | 2013 | LWT-Food Science and Technology2013，50，1： | 1 |
| 9 | Studies on the morphological and rheological properties of granular cold water soluble corn and potato starches显示文摘 | Jaspreet Singh Narpinder Singh | 2003 | Food Hydrocolloids2003，17，： | 1 |
| 10 | Dynamics of the arch-type reconfigurable machine tool显示文摘 | Jaspreet Dhupia Bartosz Powalka Reuven Katz A. Galip Ulsoy | 2006 | International Journal of Machine Tools and Manufacture2006，，2： | 1 |
| 11 | Disparities in the Use of Sacral Neuromodulation among Medicare Beneficiaries显示文摘 | Melissa A. Laudano Stephan Seklehner Jaspreet Sandhu W. Stuart Reynolds Kelly A. Garrett Jeffrey W. Milsom Alexis E. Te Steven A. Kaplan Bilal Chughtai Richard K. Lee | 2015 | The Journal of Urology2015，，： | 1 |
| 12 | Antidiabetic and antioxidant potential of Emblica officinalis Gaertn leaves extract in streptozotocin-induced type-2 diabetes mellitus ( T2DM ) rats 显示文摘 | Parminder Nain Vipin Saini Jaspreet Nain | 2012 | J Ethnopharmacol2012，142，1： | 1 |
| 13 | Studies on the morphological,thermal and rheological properties of starch separated from some Indian potato cultivars显示文摘 | Jaspreet Singh Narpinder Singh | 2001 | Food Chemistry2001，75，： | 1 |
| 14 | Visual function and vision-related quality of life after macular hole surgery with short-duration, 3-day face-down positioning显示文摘 | Jaspreet Rayat David R.P. Almeida Michel Belliveau Jonathan Wong Jeffrey Gale | 2011 | Canadian Journal of Ophthalmology/Journal canadien d’ophtalmologie2011，，5： | 1 |
| 15 | Morphological,thermal and rheological properties of starches from different botanical sources显示文摘 | NARPINDER S JASPREET S LOVEDEEP K | 2003 | Food Chemistry2003，81，2： | 1 |
| 16 | Rice Germination and Its Impact on Technological and Nutritional Properties:A Review显示文摘Grain germination is a process involving numerous factors that influence the biochemical processes inside the plant cells.This review covered the abiotic factors that lead to the germination and significantly impact the nutritional properties and digestion behavior of rice grains.The macro-and micro-nutrients can be changed depending on the intensity of the applied variables during germination.For instance,germination time can increase the protein content in the grain and concurrently reduce its protein digestibility.In most cases,the number of bioactive compounds present in rice grains are increased regardless of germination conditions.Germination can promote the complexation of nutrients and thus negatively interfere with the digestibility of macronutrients.This review highlighted the influence of the germination process on the nutritional quality of rice grains,providing information about the germination conditions and their impacts on the anabolic and catabolic reactions of the grain,emphasizing the health benefits. | LucasÁvila do NASCIMENTO Abhilasha ABHILASHA Jaspreet SINGH Moacir Cardoso ELIAS Rosana COLUSSI | 2022 | Rice science2022，29，3： | 1 |
| 17 | Morphological,thermal and rheological properties of star-ches from different botanical sources显示文摘 | NarpinderSingh Jaspreet Singh Lovedeep Kaur | 2003 | Food Chemistry2003，81，： | 1 |
| 18 | Bioadhesive mierospheres as a controlled drug delivery system显示文摘 | Jaspreet KV Tambwekar K Garg S | 2003 | Int J Pharm2003，255，12： | 1 |
| 19 | Studies on the Morphological and Rheological Properties of Granular Cold Water Soluble Com and Potato Starches 显示文摘 | Jaspreet Singh Narpinder Singh | 2003 | Food Hydrocolloids2003，17，1： | 1 |
| 20 | Effect of cross-linking on some properties of potato starches显示文摘 | Lovedeep K Jaspreet S | 2006 | Science of Food and Agriculture2006，86，： | 1 |
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