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| 1 | Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low-and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs显示文摘Background: Bacillus spp. seem to be an alternative to antimicrobial growth promoters for improving animals' health and performance. However, there is little information on the effect of Bacillus spp. in combination with different dietary crude protein(CP) levels on the ileal digestibility and microbiota composition. Therefore, the objective of this study was to determine the effect of Bacillus spp. supplementation to low-(LP) and high-protein diets(HP) on ileal CP and amino acid(AA) digestibility and intestinal microbiota composition.Methods: Eight ileally cannulated pigs with an initial body weight of 28.5 kg were randomly allocated to a rowcolumn design with 8 pigs and 3 periods of 16 d each. The assay diets were based on wheat-barley-soybean meal with two protein levels: LP(14% CP, as-fed) and HP diet(18% CP, as-fed). The LP and HP diets were supplemented with or without Bacil us spp. at a level of 0.04%(as-fed). The apparent ileal digestibility(AID) and standardized ileal digestibility(SID) of CP and AA was determined. Bacterial community composition from ileal digesta was analyzed by Illumina amplicon sequencing and quantitative real-time PCR. Data were analyzed as a 2 × 2 factorial design using the GLIMMIX procedures of SAS.Results: The supplementation with Bacillus spp. did not affect both AID and SID of CP and AA in growing pigs.Moreover, there was no difference in AID of CP and AA between HP and LP diets, but SID of cystine, glutamic acid,glycine, and proline was lower(P < 0.05) in pigs fed the HP diets. The HP diets increased abundance of Bifidobacterium spp. and Lactobacillus spp.,(P < 0.05) and by amplicon sequencing the latter was identified as predominant genus in microbiota from HP with Bacillus spp., whereas dietary supplementation of Bacillus spp. increased(P < 0.05) abundance of Roseburia spp.Conclusions: The HP diet increased abundance of Lactobacillus spp. and Bifidobacterium spp.. The supplementation of Bacil us spp. resulted in a higher abundance of healthy gut associated bacteria without affecting ileal CP and AA digestibility, whereas LP diet may reduce the flow of undigested protein to the large intestine of pigs. | Chanwit Kaewtapee Katharina Burbach Georgina Tomforde Thomas Hartinger Amelia Camarinha-Silva Sonja Heinritz Jana Seifert Markus Wiltafsky Rainer Mosenthin Pia Rosenfelder-Kuon | 2017 | Journal of Animal Science and Biotechnology2017，8，3： | 5 |
| 2 | Does intra-ruminal nitrogen recycling waste valuable resources? A review of major players and their manipulation显示文摘Nitrogenous emissions from ruminant livestock production are of increasing public concern and, together with methane, contribute to environmental pollution. The main cause of nitrogen-(N)-containing emissions is the inadequate provision of N to ruminants, leading to an excess of ammonia in the rumen, which is subsequently excreted. Depending on the size and molecular structure, various bacterial, protozoal and fungal species are involved in the ruminal breakdown of nitrogenous compounds(NC). Decelerating ruminal NC degradation by controlling the abundance and activity of proteolytic and deaminating microorganisms, but without reducing cellulolytic processes, is a promising strategy to decrease N emissions along with increasing N utilization by ruminants. Different dietary options, including among others the treatment of feedstuffs with heat or the application of diverse feed additives, as well as vaccination against rumen microorganisms or their enzymes have been evaluated. Thereby, reduced productions of microbial metabolites, e.g. ammonia, and increased microbial N flows give evidence for an improved N retention. However, linkage between these findings and alterations in the rumen microbiota composition, particularly NC-degrading microbes, remains sparse and contradictory findings confound the exact evaluation of these manipulating strategies, thus emphasizing the need for comprehensive research. The demand for increased sustainability in ruminant livestock production requests to apply attention to microbial N utilization efficiency and this will require a better understanding of underlying metabolic processes as well as composition and interactions of ruminal NC-degrading microorganisms. | Thomas Hartinger Nina Gresner Karl-Heinz Südekum | 2018 | Journal of Animal Science and Biotechnology2018，9，3： | 5 |
| 3 | Discrimination of the irritancy potential of surfactants in vitro by two cytotoxicity assays using normal human keratinocytes,HaCaT cells and 3T3 mouse fibroblasts:correlation with in vivo data from a soap chamber assay显示文摘 | KORTING H C HERZINGER T HARTINGER A | 1994 | Dermatol Sci1994，7，2： | 1 |
| 4 | Extracorporeal shock wave therapy (ESWT) for wound healing: technology, mechanisms, and clinical efficacy 显示文摘 | Mittermayr R Antonic V Hartinger J | 2012 | Wound Repair Regen2012，20，4： | 1 |
| 5 | Pulmonary passage is a major obstacle for intravenous stem cell delivery:the pulmonary first-pass effect显示文摘 | Fischer UM Harting MT Jimenez F | | 0，，05： | 1 |
| 6 | Location of pulmonary metastasis in pediatric osteosarcoma is predictive of outcome显示文摘 | Phillip A. Letourneau Lianchun Xiao Matthew T. Harting Kevin P. Lally Charles S. Cox Richard J. Andrassy Andrea A. Hayes-Jordan | 2011 | Journal of Pediatric Surgery2011，，7： | 1 |
| 7 | Variances of GPS phase observations:The SIGMA-model显示文摘 | HARTINGER H BRUNNER F K | 1999 | GPS Solutions1999，24，： | 1 |
| 8 | GPS Signal Diffraction Modelling: the SIGMA-D Model显示文摘 | Brunner F K Hartinger H | 1999 | Journal of Geodesy1999，73，： | 1 |
| 9 | Real-time management of faulty electrodes in electrical impedance tomography显示文摘 | Hartinger A A Guardo R Adler A | 2009 | IEEE Trans Biomed Eng2009，56，2： | 1 |
| 10 | Improving gastroschisis outcomes: Does birth place matter?显示文摘 | Kate B. Savoie Eunice Y. Huang Shahroz K. Aziz Martin L. Blakely Sid Dassinger Amanda R. Dorale Eileen M. Duggan Matthew T. Harting Troy A. Markel Stacey D. Moore-Olufemi Sohail R. Shah Shawn D. St. Peter Koujen Tsao Deidre L. Wyrick Regan F. Williams | 2014 | Journal of Pediatric Surgery2014，，： | 1 |
| 11 | Systemic-and barrier contraceptives for the dermatologist:a review显示文摘 | Chan CS Harting M Rosen T | | 0，，08： | 1 |
| 12 | Treatment issues in pediatric gastric adenocarcinoma显示文摘 | Matthew T. Harting Martin L. Blakely Cynthia E. Herzog Kevin P. Lally Jaffer A. Ajani Richard J. Andrassy | 2004 | Journal of Pediatric Surgery2004，，8： | 1 |
| 13 | Variance of GPS Phase Observations : The SIGMA-delta Model 显示文摘 | H Hartinger F K Brunner | 1999 | GPS Solutions1999，2，4： | 1 |
| 14 | Composide grafts of auto-genic cultured epidermis and glycerol-preserved allogenic dermis for definitive coverage of full - thickness burn wounds ; case reports显示文摘 | Schiojet WA Hartinger A Hencdel G | 1994 | Burns1994，20，： | 1 |
| 15 | GPS signal diffraction modelling: the stochastic SIGMA-δ model显示文摘 | F. K. Brunner H. Hartinger L. Troyer | | 0，1999，： | 1 |
| 16 | Interactions of antitumor metallodrugs with serum proteins:Advances in characterization using modern analytical methodology显示文摘 | TIMERBAEV A R HARTINGER C G ALEKSENKO S S | 2006 | Chem Rev2006，106，： | 1 |
| 17 | Bioorganometallic Chemistry From Teaching Paradigms to Medicinal Applications显示文摘 | Hartinger C G Dyson P J | 2009 | Chem Soc Rev2009，38，2： | 1 |
| 18 | Variances of GPS Phase Observations: The SIGMA-? Model显示文摘 | H. Hartinger F. K. Brunner | | 0，1999，： | 1 |
| 19 | On the distribution of dividend payments and the discounted penalty function in risk model with linear dividend barrier显示文摘 | Albrecher H Hartinger J Tichy R F | 2005 | Scandinavian Actuarial Journal2005，2，： | 1 |
| 20 | Interactions of antitumor metallodrugs with serum proteins:Advances in characterization using modern analytical methodology显示文摘 | TIMERBAEV A R HARTINGER C G ALEKSENKO S S | 2006 | Chemical Reviews2006，106，6： | 1 |
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