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| 1 | Quantitative determination of AI-2 quorum-sensing signal of bacteria using high performance liquid chromatography–tandem mass spectrometry显示文摘Autoinducer 2(AI-2), an important bioactive by-product of the Lux S-catalyzed S-ribosylhomocysteine cleavage reaction in the activated-methyl-cycle, has been suggested to serve as a universal intra- and inter-species signaling molecule. The development of reliable and sensitive methods for quantitative determination of AI-2 is highly desired.However, the chemical properties of AI-2 cause difficulty in its quantitative analysis.Herein, we report a high performance liquid chromatography-tandem mass spectrometric method that enables reproducible and sensitive measurement of AI-2 concentrations in complex matrixes. 4,5-Dimethylbenzene-1,2-diamine(DMBDM), an easy-to-obtain commercial reagent, was used for the derivatization treatment. The assay was linear in the concentration range of 1.0–1000 ng/m L(R^2= 0.999) and had a lower limit of quantification of0.58 ng/m L. The method exhibited several advantages, e.g., high selectivity, wide linear response range, and good sensitivity. Furthermore, the effectiveness of the method was further validated through measuring AI-2 concentrations in the cell-free culture supernatant from Escherichia coli wild type. | Fang Xu Xiangning Song Peijie Cai Guoping Sheng Hanqing Yu | 2017 | Journal of Environmental Sciences2017，29，2： | 8 |
| 2 | Field Modeling Method for Identifying Urban Sphere of Influence:A Case Study on Central China显示文摘With rapid development of urbanization and regional interaction and interdependence,regional urban agglomeration planning becomes more and more important in China,in order to promote integrated development of various cities with close interrelationship.However,it is still arguable academically on how to define the boundary or which cities to be included for the urban agglomeration of a region.This paper aims to shed lights on how to identify urban spheres of influence scientifically by introducing field modeling method and by practicing a case study on 168 cities in Central China.In our field modeling method,the influence intensities of cities were measured by a comprehensive index and urban spheres of influence were represented spatially by field intensity.Then,their classification and spatial distribution characteristics of study area in 2007 were identified and explored by using GIS and statistical methods.The result showed that:1) Wuhan is the absolute dominant city in Central China;2) the provincial capital cities dominate their own provinces and there are no other lower grade agglomeration centers;and 3) the basic types of organization form of urban sphere of influence are single-polar type,agglomeration type,close-related group type and loose-related group type. | DENG Yu LIU Shenghe WANG Li MA Hanqing WANG Jianghao | 2010 | Chinese Geographical Science2010，20，4： | 7 |
| 3 | NUMERICAL STUDIES ON RESONANT AND ENHANCEMENT EFFECTS FOR COUPLING OF MICROWAVE PULSES INTO NARROW SLOTS显示文摘In this paper, modifications to the finite-difference time-domain(FD-TD) method for modeling microwave pulse coupling into a slot, which is much narrower than one conventional FD-TD cell, are discussed. The coupling process of microwave pulse into a slot is studied by using the modified FD-TD method, and the dependence of microwave coupling on slot sizes, the carrier frequencies and the polarization directions of the incident waves is analysed. Resonant and enhancement effects which occur in this process are observed. The condition at which the resonant effect takes place is also presented. | Wang Jianguo Yu Hanqing Liu Guozhi Chen Yusheng Fan Ruyu (Northwest Institute of Nuclear Technology, Branch 15, P. O. Box 69, Xi’an 710024)Ge Debiao(Department of Physics, Xidian University, Xi’an 710071) | 1998 | Journal of Electronics(China)1998，15，2： | 6 |
| 4 | Nonlinear health evaluation for lithium-ion battery within full-lifespan显示文摘Lithium-ion batteries(LIBs), as the first choice for green batteries, have been widely used in energy storage, electric vehicles, 3C devices, and other related fields, and will have greater application prospects in the future. However, one of the obstacles hindering the future development of battery technology is how to accurately evaluate and monitor battery health, which affects the entire lifespan of battery use. It is not enough to assess battery health comprehensively through the state of health(SoH) alone, especially when nonlinear aging occurs in onboard applications. Here, for the first time, we propose a brand-new health evaluation indicator—state of nonlinear aging(SoNA) to explain the nonlinear aging phenomenon that occurs during the battery use, and also design a knee-point identification method and two SoNA quantitative methods. We apply our health evaluation indicator to build a complete LIB full-lifespan grading evaluation system and a ground-to-cloud service framework, which integrates multi-scenario data collection, multi-dimensional data-based grading evaluation, and cloud management functions. Our works fill the gap in the LIBs’ health evaluation of nonlinear aging, which is of great significance for the health and safety evaluation of LIBs in the field of echelon utilization such as vehicles and energy storage. In addition, this comprehensive evaluation system and service framework are expected to be extended to other battery material systems other than LIBs, yet guiding the design of new energy ecosystem. | Heze You Jiangong Zhu Xueyuan Wang Bo Jiang Hao Sun Xinhua Liu Xuezhe Wei Guangshuai Han Shicong Ding Hanqing Yu Weihan Li Dirk Uwe Sauer Haifeng Dai | 2022 | Journal of Energy Chemistry2022，31，9： | 4 |
| 5 | Transmission line fault-cause identification method for large-scale new energy grid connection scenarios显示文摘The accurate fault-cause identification for overhead transmission lines supports the operation and maintenance personnel in formulating targeted maintenance strategies and shortening the time of inspecting faulty lines.With the goal of achieving“carbon peak and carbon neutrality”,the schemes for clean energy generation have rapidly developed.Moreover,new energy-consuming equipment has been widely connected to the power grid,and the operating characteristics of the power system have significantly changed.Consequently,these have impacted traditional fault identification methods.Based on the time-frequency characteristics of the fault waveform,new energy-related parameters,and deep learning model,this study proposes a fault identification method suitable for scenarios where a high proportion of new energy is connected to the power grid.Ten parameters related to the causes of transmission line fault and new energy connection scenarios are selected as model characteristic parameters.Further,a fault identification model based on adaptive deep belief networks was constructed,and its effect was verified by field data. | Hanqing Liang Xiaonan Han Haoyang Yu Fan Li Zhongjian Liu Kexin Zhang | 2022 | Global Energy Interconnection2022，5，4： | 3 |
| 6 | THE PREPARATION OF CROSSLINKED N,O-CARBOXYMETHYL CHITOSAN RESINSAND THEIR ADSORPTION PROPERTIESFOR TRIGLYCERIDE IN SERUM显示文摘Crosslinked N, O-carboxymethyl chitosan resins which can selectively adsorb triglyceride, were prepared by the reaction of N, O- carboxymethyl chitosan with glutaraldehyde solution. Adsorption experiments were performed by adding the resins to the serum. The results showed that this type of adsorbent could cut down the concentration of triglyceride in serum by 56. 4% (3. 35mg/gresin ) at most, while concentration of the total protein (TP ) decreased only by 10. 9% at least, so this novel adsorbent can be used to cure hypertriglyceridemia by hemoperfusion in the future. | Yu Yihau He Binglin(Institute of Polymer Chemistry, The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Nankai University, Tianjin 300071, P. R. China)Gu Hanqing(Tianjin Institute of Urological Surgery) | 1997 | Chinese Journal of Reactive Polymers1997，6，1： | 2 |
| 7 | Mechanisms behind the accelerated extracellular electron transfer in Geobacter sulfurreducens DL-1 by modifying gold electrode with self-assembled monolayers显示文摘有羧基的酸的电极表面的修正终止了自我装配的单层(SAM ) 被发现了是一条有效途径在电极表面上改进电气化学地活跃的细菌(EAB ) 的细胞外的电子转移(EET ) 的 alkanethiol,但是在这后面的内在的机制提高了 EET 遗体不清楚。在这个工作,金电极由 mercapto 醋性的酸和 mercaptoethylamine 修改了(Au-COOH, Au-NH 2) 在与 Geobacter sulfurreducens DL-1,和他们的电气化学的表演接种的微生物引起的电解房间(MEC ) 被用作阳极,细菌电极相互作用被调查。结果证明 Fe (CN )63-/4- 氧化还原作用反应在 Au 或 Au-COOH 上比那与更高的率和一个更低的电阻发生在 Au-NH 2 上。两个都,有 Au-COOH 和 Au-NH 2 阳极的 MEC 与赤裸的 Au 阳极比那展出了更高当前的密度。当 Au-NH 2 上的 biofilm 有更大的厚度时,在 Au-COOH 上形成的 biofilm 在赤裸的 Au 上是比那稠密的,建议在这个系统的直接 EET 的一个关键角色。这个工作建议那如此的 as-COOH and-NH 2 能提拔的功能的组由在电极表面上加速 EAB 的直接 EET 的电极性能。 | Feng ZHANG Shengsong YU Jie LI Wenwei LI Hanqing YU | 2016 | Frontiers of Environmental Science & Engineering2016，10，3： | 2 |
| 8 | Comparative study on constitutive relationship of as-cast Ti60 titanium alloy during hot deformation based on Arrhenius-type and artificial neural network models显示文摘 | Wenwen Peng Weidong Zeng Qingjiang Wang Hanqing Yu | 2013 | Materials and Design2013，，： | 2 |
| 9 | WindSat satellite comparisons with nearshore buoy wind data near the U.S. west and east coasts显示文摘Nearshore wind speeds retrieved by WindSat are validated by a comparison with the moored buoy observations near the U.S.west and east coasts.A 30 min and 25 km collection window is used for the Wind Sat wind data and buoy measurements from January 2004 to December 2014.Comparisons show that the overall root-mean-square error is better than 1.44 m/s near the U.S.coasts,and the result for the east coast is better than that for the west coast.The retrieval accuracy of the descending portions is slightly better than that of the ascending portions.Most buoy-to-buoy variations are not significantly correlated with the coastal topography,the longitude and the distance from the shore or satellite-buoy separation distance.In addition,comparisons between a polarimetric microwave radiometer and a microwave scatterometer are accomplished with the nearshore buoy observations from 2007 to 2008.The Wind Sat-derived winds tend to be lower than the buoy observations near the U.S.coasts.In contrast,the Quik SCAT-derived winds tend to be higher than the buoy observations.Overall,the retrieval accuracy of Wind Sat is slightly better than that of Quik SCAT,and these satellite-derived winds are sufficiently accurate for scientific studies. | ZHANG Lei SHI Hanqing YU Hong YI Xin | 2016 | Acta Oceanologica Sinica2016，35，7： | 2 |
| 10 | Purification and molecular weight distribution of a key exopolysaccharide component of Bacillus megaterium TF10显示文摘Extracellular polymeric substances(EPS) are organic metabolic compounds excreted by microorganisms. They largely impact microbial aggregate structures and functions.Extracellular polysaccharides(EP) in EPS are responsible for the formation of microbial aggregates. In this work, we successfully separated and characterized EP from EPS of the bacterium Bacillus megaterium TF10. Extraction of EP from EPS was optimized using Sevag's reagent. Chemical characteristics, functional groups, and molecular weight(MW) distribution of EP were compared with the harvested EPS and soluble microbial products(SMP). We found that the polymers of lower MW and free proteins were successfully removed by Sevag's reagent. The higher MW components of EPS were predominantly polysaccharides,while the polymers of lower MW tended to secrete to the supernatant and were described as SMP. A part of the proteins in the EP was polysaccharide-bonded. Our results can be further used in elucidating the complex flocculation mechanisms in which EP play a major role. | Lingling Wang Longfei Wang Qingshan Shi Hanqing Yu | 2018 | Journal of Environmental Sciences2018，30，1： | 2 |
| 11 | Coordinated regulation of active and repressive histone methylations by a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans显示文摘H3K9me2 和 H3K27me2 是与抄写压抑联系的重要 epigenetic 标记,当 H3K4me3 与抄写激活被联系时。活跃、压抑的 histone methylations 以一种互相独占的方式散布,这被显示出,但是内在的机制糟糕被理解。这里,我们识别了 ceKDM7A,一个哲学博士(植物 homeodomain )- 并且 JmjC 包含域的蛋白质,为 H3K9me2 和 H3K27me2 特定的 histone demethylase。我们进一步证明 ceKDM7A 的 PHD 领域在染色体宽的水平绑了 H3K4me3 和与 ceKDM7A co 局部性的 H3K4me3。到 H3K4me3 的 PHD 域绑定的混乱在 vivo 减少了 demethylase 活动,并且 ceKDM7A 的损失减少了它的联系目标基因的表达式。这些结果显示 ceKDM7A 被招募到倡导者到 demethylate H3K9me2 和 H3K27me2 并且通过 PHD 领域的绑定激活基因表示到 H3K4me3。因此,我们的学习识别双特性的 histone demethylase 并且提供新奇卓见进 histone methylation 的规定。 | Hanqing Lin Yiqin Wang Yanru Wang Feng Tian Pu Pu Yi Yu Hailei Mao Ying Yang Ping Wang Lulu Hu Yan Lin Yi Liu Yanhui Xu Charlie Degui Chen | 2010 | Cell Research2010，20，8： | 2 |
| 12 | Tuning of activated sludge in winter based on respirogram profiles under standard and site temperatures显示文摘Respirograms of activated sludge OUR_ x^Tand OUR_x^(20)were measured under site(T) and standard(20°C) temperatures, respectively, and the predicted standard temperature respirogram OUR_( x,cal)^(20)was also calculated using the Arrhenius equation. These respirogram profiles reveal more information than effluent quality. A decrease of OUR_ x ^(20)is a critical alarm signal for the loss of pollutant removal capacity, and a sudden increase of the predicted value OUR_( x,cal)^(20)is an alarm signal for the unrecoverable deterioration of biomass. The sign of OUR_x^(20)–OUR_(x,cal)^(20)can be used for selection of tuning strategies. For example, a negative value of OUR_x^(20)–OUR_( x,cal)^(20)indicates that doubling biomass is difficult,thus strategies such as extending the reaction time with limited available biomass is preferred. The findings in this study elucidated the respiration profile of activated sludge under changes of temperature and can be effectively used for the stable operation of Wastewater Treatment Plants under cold temperatures and seasonal variations. | Zhihua Li Zhenyu Hang Qian Zhang Shuangshuang Zhang Tianyu Zhang Hanqing Yu | 2019 | Journal of Environmental Sciences2019，31，5： | 2 |
| 13 | Formation and characterizationof aerobic granules in a sequencing batch reactor treatingsoybean-processing wastewater显示文摘 | Su Kuizu Yu Hanqing | 2005 | Environmental Scienceand Technology2005，39，8： | 1 |
| 14 | Stability of sludge flocs under shear conditions : roles of extracellular pol- ymeric substances (EPS)显示文摘 | SHENG Guoping YU Hanqing LI Xiaoyan | 2006 | Biotechnology and Bioengineer ing2006，93，6： | 1 |
| 15 | Biological hydrogen production by anaerobic sludge at various temperatures显示文摘 | MU Yang ZHENG Xianjun YU Hanqing | 2006 | International Journal of Hydrogen Energy2006，31，6： | 1 |
| 16 | Biosorption of 2,4-dichlorophenol from aqueous solution by Phanerochaete chrysosporium biomass:isotherms,kinetics and thermodynamics显示文摘 | WU Juan YU Hanqing | | 0，，01： | 1 |
| 17 | Hydrogen production from rice winery wastewater in an upflow anaerobic reactor by using mixed anaerobic culture显示文摘 | Yu Hanqing Zhu Zhenhu Hu Wenrong | 2002 | Int J Hydrogen Energy2002，27，： | 1 |
| 18 | Effect of processing parameters on hot deformation behavior and microstructural evolution during hot compression of as-cast Ti60 titanium alloy显示文摘 | Wenwen Peng Weidong Zeng Qingjiang Wang Qinyang Zhao Hanqing Yu | 2014 | Materials Science & Engineering A2014，，： | 1 |
| 19 | An empirical model for predicting the organic concentration of anaerobic filter effluents显示文摘 | Wilson F Hanqing Yu Joo Hwa Tay 1998 | 1998 | Asia Water Environs1998，，4： | 1 |
| 20 | Physical and chemical characteristics of granular activate sludge from a sequencing batch airlift reactor显示文摘 | Zheng Yuming Yu Hanqing Sheng Guoping | 2005 | Process Biochemistry2005，40，2： | 1 |
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