|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | NEW METHOD OF SIMULATING CONCENTRATED DRAIN HOLES IN SEEPAGE CONTROL ANALYSIS显示文摘In this paper, based upon the basic solution of sink, the approximate solution of single drain hole in finite elements is derived by use of the superposition principle. Then, the theoretical solution is extended to the case of some drain holes in one finite element, and the method is used in seepage control analysis with quick convergence and high accuracy. On the other hand, if the positions of the drain holes are changed, only some control factors of drain holes are changed, but the finite element grid need not to be reformed. Therefore, the method is more suitable in optimal research of seepage control. | Zhan Mei li, Su Bao yu , Liu Jun yong, Shen Zhen zhong College of Water Conservancy and Hydropower Engineering, Hohai University, Nanjing 210098, P.R.China (Received Aug.1, 1998) | 1999 | Journal of Hydrodynamics1999，11，3： | 22 |
| 3 | Influence of whole peptidoglycan of bifidobacterium on cytotoxic effectors produced by mouse peritoneal macrophages显示文摘INTRODUCTIONBifidobacteria are physiologically beneficial bacteria which are perdominant in human intestine ,and possess the most important functions .They play an important role in maintaining microbial balance of the intestine .Furthermore , their presence is thought to be an important indication of health of the body [1-4].Whole peptidoglycan ( WPG) is the major component in the cell wall of bifidobacterium ,which is also a biological responsemodifier with nontoxic side dffcets. | Li Sheng Wang~1 Hui Ming Zhu~1 Dian Yuan Zhou~2 Yu Lin Wang~1 Wan Dai Zhang~2 ~1Departrnent of Gastroenterology,Shenzhen Municipal People’s Hospital,Jinan University of Medical Sciences,Shenzhen 518020,Guangdong Province,China ~2Chinese PLA Institute of Digestion,the First Military Medical University,Guangzhou 510515,Guangdong Province,ChinaLi Sheng Wang graduated and obtained Ph.D,from the First Military Medical University in 1998,now working at Department of Gastroenterology,Shenzhen Municipal People’s Hospital.Jinan University of Medical Sciences.having 35 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 15 |
| 4 | Influence of Long-Term Fertilization with Different Mineral Fertilizers and Farmyard Manure on Some Soil Chemical Properties and Crop Yields显示文摘Longtermexperimentshaveaspecialvalueforprovidinginformationtoguideagriculturaldevelopments.Asearlyasin1843,LawesandGilbertes... | LIU DEHUI 1, J. LABETOWICZ 2 and L. KUSZELEWSKI 2 1 Nanjing Agricultural University, Nanjing 210095 (China) 2 Department of Agricultural Chemistry, Warsaw Agricultural University, Warsaw 02 528 (Poland) (Received February 15, 1998 r | 1998 | Pedosphere1998，8，3： | 8 |
| 5 | Effect of Cultural Measures on Nutrient Contellts in Rice Plants with Erect Panicles显示文摘Since1981,newricevarietieswitherectpanicles,suchasLiaojing5,Shennong91,Liaojing326andShennong8801,havebeenbredinLiaoningProvinceofChina.Experimelltalresultsindicatedthatthegrainyieldsofvarietieswitherectpaniclesnotonlyexceededthoseofcultivarswithcurv... | WANG BOLUN LIU XINAN YU GUIRUI WANG SHUI and ZOU BANGJI(Shenyang Agricultural University, Shenyang 110161 (China))(Institute Of Applied Ecolopy, the Chinese Academy of Sciences, Shenyany 110015 (China))(Received November 8, 1998 revised December 21, 1998) | 1999 | Pedosphere1999，9，3： | 3 |
| 6 | Phosphate Rock Fertilizer in Acid Soils: Comparing Phosphate Extraction Methods for Measuring Dissolution显示文摘INTRODUCTIONTheextentofdissolutionofphosphaterock(PR)insoilhasbeendeterminedbyeithermeasuringtheincreaseintheamountsofsoluble... | T. S. ANSUMANA KAWA and WANG GUANGHUO Department of Soil Science and Applied Chemistry, College of Natural Resources and Environmental Sciences, Zhejiang Agricultural University, Hangzhou 310029 (China) (Received March 13, 1998 revised April 22, 199 | 1998 | Pedosphere1998，8，3： | 3 |
| 7 | Controlled Radical Polymerization of Styrene in the Presence of Different Copper Complexes and Organic Halides显示文摘The polymerization behaviors of Styrene (St) in the presence of CuX/L [X=Cl or Br; L= 2,2 bipyridine (bpy), 1,10 phenanthroline (phen) or 4,7 diphenyl 1,10 phenanthroline (DPP) ] and R X (R=trichloromethyl, benzyl or allyl; X=Cl or Br) have been studied and examined. In a CuCl/bpy/RCl/St system, a bimodal GPC peak at the early stage of polymerization was observed, and a concept of multi active species was proposed to explain this phenomenon. In a CuCl/phen (DPP)/RCl/St system, the \%M\%\-n of polystyrene (PS) increased linearly with St conversion and ln[M] o/[M] also increased linearly with time, indicating the living nature of this system. Furthermore, the stability of the propagating active species in a CuBr/phen/RBr/St system is higher than that in the CuBr/phen/RBr/St system. | CHENG Guang lou, HU Chun pu \{**\} and YING Sheng kang (Laboratory of Living Polymerization, East China University of Science and Technology, Shanghai 200237, P.R. China) (Received August 28, 1998) | 1999 | Chemical Research in Chinese Universities1999，15，4： | 2 |
| 8 | Recent developments in biochemistry of the plant lipoxygenase pathway显示文摘 | Alexander G 1998 | 1998 | Prog Lipid Res1998，37，5： | 2 |
| 9 | An Adaptive Identification and Control SchemeUsing Radial Basis Function Networks显示文摘In this paper, adaptive identification and control of nonlinear dynamical systems are investigated using radial basis function networks (RBF). Firstly, a novel approach to train the RBF is introduced, which employs an adaptive fuzzy generalized learning vector quantization (AFGLVQ) technique and recursive least squares algorithm with variable forgetting factor (VRLS). The AFGLVQ adjusts the centers of the RBF while the VRLS updates the connection weights of the network. The identification algorithm has the properties of rapid convergence and persistent adaptability that make it suitable for real-time control. Secondly, on the basis of the one-step ahead RBF predictor, the control law is optimized iteratively through a numerical stable Davidon’s least squares-based (SDLS) minimization approach. Four nonlinear examples are simulated to demonstrate the effectiveness of the identification and control algorithms. | Chen Zengqiang He Jiangfeng Yuan Zhuzhi (Department of Computer and System Science, Nankai University, Tianjin 300071, P. R. China)(Received July 12, 1998) | 1999 | Journal of Systems Engineering and Electronics1999，10，1： | 2 |
| 10 | NUMERICAL SOLUTION OF 2D FLOW USING A LOCAL COORDINATE SYSTEM显示文摘This paper is concerned with a new numerical method of two dimensional flow. The governing system of differential equations is transformed into an equivalent system applied over a square grid network in order to overcome the difficulties and inaccurancies associated with the determination of characteristics near the flow boundaries. The MacCormack two step explicit scheme with second order accurancy is used for the solution of the transformed system of equations. Comparison between computed and experimental data shows a satisfactory agreement. | Wei Wen li, Wang De yi, Liu Yu ling, Tan Li xin Xi′an University of Technology, Xi′an 710048, P.R.China (Receied Nov.30,1998) | 1999 | Journal of Hydrodynamics1999，11，3： | 2 |
| 11 | Changes of Protective Enzymes and Accumulation of Polyamines Induced by Boron Deficiency in Roots of Rape Seedlings显示文摘Boronisanesentialmicronutrientrequiredforgrowthanddevelopmentofhigherplants.SeveralreportsexpresedtheopinionthatBdirectlyafec... | XIAO FENGHUI Department of Biotechnology, Yunnan Agricultural University, Kunming 650201 (China) (Received April 23, 1998 revised June 2, 1998) | 1998 | Pedosphere1998，8，3： | 2 |
| 12 | Sectional distribution of salinity and its indication of Kuroshio'sintrusion in the southern Taiwan Strait and northeastern South China Sea late summer, 1994显示文摘Using the CTD data investigated at about 330 stations in the southern Taiwan Strait, the northeastern South China Sea and their adjacent seas in August and September of 1994, this paper anayses the sectional distributions of salinity in 10 selected sections and discusses the possibility of the Kuroshio’s intrusion into the northeastern South China Sea and the southern Taiwan Strait. The results show that: as the main stream of Kuroshio passes by the northern Luzon Strait during the survey period, the 'Kuroshio-influenced water' with some hydrological features of the Kuroshio may extend through the Luzon Strait to the sea areas near both the Dongsha Islands and the Taiwan Shoal of the southern Taiwan Strait. | Hu Jianyu Liang Hongxing and Zhang Xuebin 1. Institute of Subtropical Oceanography, Xiamen University, Xiamen 361005, China 2. Fujian Institute of Oceanology, Xiamen 361012, China (Received June 3, 1998 accepted August 8, 1998) | 1999 | Acta Oceanologica Sinica1999，18，2： | 2 |
| 13 | Study on topographic compensation model of the Okinawa Trough I. Calculation of theoretical isostatic response function显示文摘Based on regional compensation and experimental isostasy, the calculating formula of theoretical isostatic response function is deduced when the loads on top correlate with loads from below. A variety of curves of theoretical isostatic response function were calculated with different effective elastic thickness of the plate and by using different proportions between loads on top and loads from below. And preliminary discussion is made on shapes of the curves. | Liu Baohua Wu Jinlong Xin Baisen and Peng Chao 1. First Institute of Oceanography, State Oceanic Administration, Qingdao 266003, China 2. Ocean University of Qingdao, Qingdao 266003, China (Received October 12, 1997 accepted July 17, 1998) | 1999 | Acta Oceanologica Sinica1999，18，2： | 2 |
| 14 | Study on topographic compensation model of the Okinawa Trough Ⅱ. Geological interpretation of isostatic response function of the Okinawa Trough显示文摘Based on regional compensation model and experimental isostasy, the isostatic response function of the Okinawa Trough is calculated by using gravity and topographic data. The results are shown as follows: the effective elastic thickness of the plate and compensation depth in the southern Okinawa Trough is obviously greater than those in the middle Okinawa Trough. In reference with other geological and geophysical data, the differences between the two portions are explained to be caused mainly by their differences in temperature of the lithosphere and the compensation mechanism. | Xin Baisen Liu Baohua and Xia Zhifeng 1. Ocean University of Qingdao, Qingdao 266003, China 2. First Institute of Oceanography, State Oceanic Administration, Qingdao 266003, China (Received October 12, 1997 accepted July 17, 1998) | 1999 | Acta Oceanologica Sinica1999，18，2： | 2 |
| 15 | Organic molecular tracers for particulate air pollution sources 显示文摘 | 1998 | 1998 | Trends in Analytical Chemistry1998，17，6： | 1 |
| 16 | New chemically modified polymeric resin for solid-phase extraction of pesticides and phenolic compounds from water显示文摘 | Galia M Marce R M 1998 | 1998 | J Chromatogr A1998，803，： | 1 |
| 17 | Study of the tectonic movement of Guizhou in xishan period 显示文摘 | Qin SR Liu AM 1998 | 1998 | Guizhou Geology1998，15，2： | 1 |
| 18 | The influence of food quality on the phototaetie behavior of Daphnia magna straus显示文摘 | Michels E De Meester L 1998 | 1998 | Hydrobiologia1998，379，： | 1 |
| 19 | Cercidiphyllum crenatum (Unger) R W Brown in der Kohlenfuhrenden Abfolge von Oberdorf N Voitsber,Steiermark显示文摘 | Kovar-Eder J Meller B Zetter R 1998 | 1998 | Mitt Ref Geol Palantol Landesmus Joanneum1998，2，： | 1 |
| 20 | Cadmium uptake by barley as affected by Cd sources and pH levels显示文摘 | Singh B R Myhr K 1998 | 1998 | Geoderma1998，84，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员