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| 1 | Isolation and characterization of H7N9 viruses from live poultry markets--Implication of the source of current H7N9 infection in humans显示文摘On March 31, 2013, the National Health and Family Planning Commission announced that human infections with a previously undescribed influenza A (H7N9) virus had occurred in Shanghai and Anhui Province, China. To investigate the possible origins of the H7N9 viruses causing these human infections, we collected 970 samples, including drinking water, soil, and cloacal and tracheal swabs of poultry from live poultry markets and poultry farms in Shanghai and Anhui Province. Twenty samples were positive for the H7N9 influenza virus. Notably, all 20 viruses were isolated from samples collected from live poultry markets in Shanghai. Phylogenetic analyses showed that the six internal genes of these novel human H7N9 viruses were derived from avian H9N2 viruses, but the ancestor of their HA and NA genes is uncertain. When we examined the phylogenetic relationship between the H7N9 isolates from live poultry markets and the viruses that caused the human infections, we found that they shared high homology across all eight gene segments. We thus identified the direct avian origin of the H7N9 influenza viruses that caused the human infections. Importantly, we observed that the H7N9 viruses isolated from humans had acquired critical mutations that made them more 'human-like'. It is therefore imperative to take strong measures to control the spread of H7N9 viruses in birds and humans to prevent further threats to human health. | SHI JianZhong DENG GuoHua LIU PeiHong ZHOU JinPing GUAN LiZheng LI WenHui LI XuYong GUO Jing WANG GuoJun FAN Jun WANG JinLiang LI YuanYuan JIANG YongPing LIU LiLing TIAN GuoBin LI ChengJun CHEN HuaLan | 2013 | Chinese Science Bulletin2013，58，16： | 85 |
| 2 | Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China显示文摘The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza. | Pengfei Cui Xianying Zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen | 2022 | Science China(Life Sciences)2022，65，4： | 12 |
| 3 | Differentiation of human adipose-derived stem cells into neuron-like cells by Radix Angelicae Sinensis显示文摘Human adipose tissues are an ideal source of stem cells.It is important to find inducers that can safely and effectively differentiate stem cells into functional neurons for clinical use.In this study,we investigate the use of Radix Angelicae Sinensis as an inducer of neuronal differentiation.Primary human adipose-derived stem cells were obtained from adult subcutaneous fatty tissue,then pre-induced with 10%Radix Angelicae Sinensis injection for 24 hours,and incubated in serum-free Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 containing 40%Radix Angelicae Sinensis to induce its differentiation into neuron-like cells.Butylated hydroxyanisole,a common inducer for neuronal differentiation,was used as the control.After human adipose-derived stem cells differentiated into neuron-like cells under the induction of Radix Angelicae Sinensis for 24 hours,the positive expression of neuron-specific enolase was lower than that of the butylated hydroxyanisole-induced group,and the expression of glial fibrillary acidic protein was negative.After they were induced for 48 hours,the positive expression of neuron specific enolase in human adipose-derived stem cells was significantly higher than that of the butylated hydroxyanisole-induced group.Our experimental findings indicate that Radix Angelicae Sinensis can induce human adipose-derived stem cell differentiation into neuron-like cells and produce less cytotoxicity. | Qiaozhi Wang Lile Zhou Yong Guo Guangyi Liu Jiyan Cheng Hong Yu | 2013 | Neural Regeneration Research2013，8，35： | 3 |
| 4 | Observation of a bi-critical point between antiferromagnetic and superconducting phases in pressurized single crystal Ca0.73La0.27FeAs2显示文摘One of the most strikingly universal features of the high-temperature superconductors is that the superconducting phase emerges in the close proximity of the antiferromagnetic phase, and the interplay between these two phases poses a long-standing challenge. It is commonly believed that, as the antiferromagnetic transition temperature is continuously suppressed to zero, there appears a quantum critical point, around which the existence of antiferromagnetic fluctuation is responsible for the development of the superconductivity. In contrast to this scenario, we report the observation of a bi-critical point identified at 2.88 GPa and 26.02 K in the pressurized high-quality single crystal Ca_(0.73)La_(0.27)FeAs_2 by complementary in-situ high pressure measurements. At the critical pressure, we find that the antiferromagnetism suddenly disappears and superconductivity simultaneously emerges at almost the same temperature, and that the external magnetic field suppresses the superconducting transition temperature but hardly affects the antiferromagnetic transition temperature. | Yazhou Zhou ShanJiang Qi wu Vladimir A.Sidorov Jing Guo Wei Yi Shan Zhang Zhe Wang HonghongWang shu Cai Ke Yang Sheng Jiang Aiguo Li Ni Ni Guangming Zhang Liling Sun Zhongxian Zhao | 2017 | Science Bulletin2017，62，12： | 3 |
| 5 | Fast elimination of malaria by infectious source eradication with artemisinin-based compound显示文摘Malaria is one of the three most hazardous infectious diseases(HIV/AIDS,Malaria and tuberculosis)in the world.Since 1960,falciparum malaria gradually became resistant to original antimalarial drugs such as Chloroquine and Fansidar.With the global spread of drug-resistant falciparum malaria,morbidity and mortality increased greatly.In | Liling Feng Xiaoli Luo Yaofang Zhou Xingbo Guo Guoqiao Li | 2016 | Science China(Life Sciences)2016，59，1： | 2 |
| 6 | H7N9 Influenza Viruses Are Transmissible in Ferrets by Respiratory Droplet显示文摘 | Qianyi Zhang Jianzhong Shi Guohua Deng Jing Guo Xianying Zeng Xijun He Huihui Kong Chunyang Gu Xuyong Li Jinxiong Liu Guojun Wang Yan Chen Liling Liu Libin Liang Yuanyuan Li Jun Fan Jinliang Wang Wenhui Li Lizheng Guan Qimeng Li Huanliang Yang Pucheng Che | 2013 | Science2013，，6144： | 1 |
| 7 | 显示文摘 | Shi Kunyu Guo Liling Liu Hanxing | 2006 | Solid State Phenom2006，111，： | 1 |
| 8 | 显示文摘 | Dai Yadong Hu Mingjie Guo Liling | 2006 | Solid State Phenom2006，111，： | 1 |
| 9 | 显示文摘 | Liu Hanxing Guo Liling Cao Minhe | 2004 | Materials Science and Engineering B2004，113，2： | 1 |
| 10 | 显示文摘 | Guo Liling Liu Hanxing Dai Yadong | 2007 | J Phys Chem Solids2007，68，9： | 1 |
| 11 | 显示文摘 | Dai Yadong Guo Liling Hu Mingjie | 2005 | Solid-State Chem Inorg Mater2005，848，： | 1 |
| 12 | 显示文摘 | Guo Liling Dai Yadong Hu Mingjie | 2005 | Solid-State Chem Inorg Mater2005，848，： | 1 |
| 13 | H7N9 Influenza Viruses Are Transmissible in Ferrets by Respiratory Droplet显示文摘 | Qianyi Zhang Jianzhong Shi Guohua Deng Jing Guo Xianying Zeng Xijun He Huihui Kong Chunyang Gu Xuyong Li Jinxiong Liu Guojun Wang Yan Chen Liling Liu Libin Liang Yuanyuan Li Jun Fan Jinliang Wang Wenhui Li Lizheng Guan Qimeng Li Huanliang Yang Pucheng Che | 2013 | Science2013，，6144： | 1 |
| 14 | The structural significance of the interlayer distances of the layered hybrids (CnH2n+1NH3)2MCl4显示文摘 | GUO Liling XU Shengdong ZHAO Guanghui | 2012 | J Phys Chem Solids2012，73，： | 1 |
| 15 | Prepa ration and characterization of the layered hybrids (CnH2n+1 NH3)2FeCl4 显示文摘 | Guo Liling Liu Hanxing Dai Yadong | 2007 | J Phys Chem Solids2007，68，9： | 1 |
| 16 | A comprehensive profile of TCF1^(+)progenitor and TCF1−terminally exhausted PD-1^(+)CD8^(+)T cells in head and neck squamous cell carcinoma:implications for prognosis and immunotherapy显示文摘The heterogeneity of exhausted T cells(Tex)is a critical determinant of immune checkpoint blockade therapy efficacy.However,few studies have explored exhausted T cell subpopulations in human cancers.In the present study,we examined samples from two cohorts of 175 patients with head and neck squamous cell cancer(HNSCC)by multiplex immunohistochemistry(mIHC)to investigate two subsets of Tex,CD8+PD1+TCF1+progenitor exhausted T cells(TCF1+Texprog)and CD8+PD1+TCF1−terminally exhausted T cells(TCF1−Texterm).Moreover,fresh tumor samples from 34 patients with HNSCC were examined by flow cytometry and immunohistochemistry to further investigate their properties and cytotoxic capabilities and their correlation with regulatory T cells(Tregs)in the tumor immune microenvironment(TIME).mIHC and flow cytometry analysis showed that TCF1−Texterm represented a greater proportion of CD8+PD1+Tex than TCF1+Texprog in most patients.TCF1+Texprog produced abundant TNFα,while TCF1−Texterm expressed higher levels of CD103,TIM-3,CTLA-4,and TIGIT.TCF1−Texterm exhibited a polyfunctional TNFα+GZMB+IFNγ+phenotype;and were associated with better overall survival and recurrence-free survival.The results also indicated that larger proportions of TCF1−Texterm were accompanied by an increase in the proportion of Tregs.Therefore,it was concluded that TCF1−Texterm was the major CD8+PD1+Tex subset in the HNSCC TIME and that these cells favor patient survival.A high proportion of TCF1−Texterm was associated with greater Treg abundance. | Dikan Wang Juan Fang Shuqiong Wen Qunxing Li Jinming Wang Lisa Yang Wenxiao Dai Huanzi Lu Junyi Guo Zhongyan Shan Wenqiang Xie Xiangqi Liu Liling Wen Jie Shen Anxun Wang Qianming Chen Zhi Wang | 2022 | International Journal of Oral Science2022，14，1： | 0 |
| 17 | Conformation of Organic Chain in Phase Transition of Hybrid(C1_2H_(25)NH_3)_2MnCl_4显示文摘The structural change in phase transition of hybrid(C 12 H 25 NH 3 ) 2 MnCl 4 was investigated.The temperature and the structures of the phase transition is investigated by thermal gravimetry(TG)and differential scanning calorimetry(DSC),infrared spectrum(IR)and X-ray diffraction(XRD).The results suggest that the phase transition is reversible and the structural change arises from the conformation change of the organic chain. The interlayer distance increases when the hybrid transforms from low temperature phase to high temperature phase.This is explained by the diffusion of gauche-bond along the organic chains and they move away from each other when the phase transition occurs.Combining the experimental data with theoretical calculation, we propose that organic chain of the hybrid in high temperature phase is the conformation of gauche-bond alternating with trans bond(noted as GTG’TGTG’TGTG’T). | GUO Liling SUN Yubing MEI Ao DAI Yadong LIU Hanxing | 2008 | Journal of Wuhan University of Technology(Materials Science)2008，23，4： | 0 |
| 18 | Mycorrhizal Networks Interacting with Litter Improves Nutrients and Growth for One Plant through the Vary of N/P Ratio under Karst Soil显示文摘Arbuscular mycorrhizae(AM)fungi affect nutrient uptake for host plants,while it is unclear how AM fungi interacting with soil litter affect plant growth and nutrient utilization through mycorrhizal networks in karst soil of deficient nutrients beyond the rhizosphere.An experiment was conducted in a microcosm composed of a planting compartment for Cinnamomum camphora seedlings with or without Glomus mosseae fungus(M+vs.M−)and an adjacent litter compartment containing or not containing additional litter material of Arthraxon hispidus(L+vs.L−),where the compartments are connected either by nylon mesh of 20μm or 0.45μm which either allow available mycorrhizal networks within the litter compartment or prevent mycelium entering into the litter compartment(N+vs.N−).Plant biomass and nutrients were measured.The results showed that the addition of litter changed the symbiotic process in mycorrhizal colonization,spore,and hyphal density,which when in association with the host plant then affected the biomass,and accumulations of N(nitrogen)and P(phosphorus)in the individual plant as well as root,stem,and leaf respectively.AM fungi increased N and P accumulations and N/P ratio in individual plants and plant tissues.A decrease of the N/P ratio of the individual plant was observed when AM fungus interacted significantly with litter through mycorrhizal networks in the litter compartment.The results indicate that the C.camphora seedlings benefited from litter in nutrient utilization of N and P through the vary of N/P ratio when accessing mycorrhizal networks.These findings suggest that mycorrhizal networks interacting with litter improve growth and nutrients of N and P for plants through the vary of N/P ratio in order to alleviate nutrient limitation under karst soil. | Liling Kang Yuejun He Lipeng Zang Jianpeng Si Ying Yang Kaiping Shen Tingting Xia Qiyu Tan Bangli Wu Yun Guo Wei Wang Qin Liang | 2021 | Phyton-International Journal of Experimental Botany2021，90，3： | 0 |
| 19 | AGO2a but not AGO2b mediates antiviral defense against infection of wild-type cucumber mosaic virus in tomato显示文摘Evolutionarily conserved antiviral RNA interference(RNAi)mediates a primary antiviral innate immunity preventing infection of broad-spectrum viruses in plants.However,the detailed mechanism in plants is still largely unknown,especially in important agricultural crops,including tomato.Varieties of pathogenic viruses evolve to possess viral suppressors of RNA silencing(VSRs)to suppress antiviral RNAi in the host.Due to the prevalence of VSRs,it is still unknown whether antiviral RNAi truly functions to prevent invasion by natural wild-type viruses in plants and animals.In this research,for the first time we applied CRISPR-Cas9 to generate ago2a,ago2b,or ago2ab mutants for two differentiated Solanum lycopersicum AGO2s,key effectors in antiviral RNAi.We found that AGO2a but not AGO2b was significantly induced to inhibit the propagation of not only VSR-deficient Cucumber mosaic virus(CMV)but also wild-type CMV-Fny in tomato;however,neither AGO2a nor AGO2b regulated disease induction after infection with either virus.Our findings firstly reveal a prominent role of AGO2a in antiviral RNAi innate immunity in tomato and demonstrate that antiviral RNAi evolves to defend against infection of natural wild-type CMV-Fny in tomato.However,AGO2a-mediated antiviral RNAi does not play major roles in promoting tolerance of tomato plants to CMV infection for maintaining health. | Liling Zhao Yingfang Chen Xingming Xiao Haiying Gao Jiamin Cao Zhongkai Zhang Zhongxin Guo | 2023 | Horticulture Research2023，10，5： | 0 |
| 20 | Establishment of cervical model of squamous cell cavity lymph node metastasis carcinoma in the oral显示文摘 | Rui Sun Liling Wu Jiangang Zhang Chuanbin Guo | 2008 | 中国口腔颌面外科杂志2008，6，B05： | 0 |
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