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| 1 | Arachidyl amido cholanoic acid improves liver glucose and lipid homeostasis in nonalcoholic steatohepatitis via AMPK and mTOR regulation显示文摘BACKGROUND Arachidyl amido cholanoic acid(Aramchol)is a potent downregulator of hepatic stearoyl-CoA desaturase 1(SCD1)protein expression that reduces liver triglycerides and fibrosis in animal models of steatohepatitis.In a phase IIb clinical trial in patients with nonalcoholic steatohepatitis(NASH),52 wk of treatment with Aramchol reduced blood levels of glycated hemoglobin A1c,an indicator of glycemic control.AIM To assess lipid and glucose metabolism in mouse hepatocytes and in a NASH mouse model[induced with a 0.1%methionine and choline deficient diet(0.1MCD)]after treatment with Aramchol.METHODS Isolated primary mouse hepatocytes were incubated with 20μmol/L Aramchol or vehicle for 48 h.Subsequently,analyses were performed including Western blot,proteomics by mass spectrometry,and fluxomic analysis with 13C-uniformly labeled glucose.For the in vivo part of the study,male C57BL/6J mice were randomly fed a control or 0.1MCD for 4 wk and received 1 or 5 mg/kg/d Aramchol or vehicle by intragastric gavage for the last 2 wk.Liver metabolomics were assessed using ultra-high-performance liquid chromatography-time of flight-MS for the determination of glucose metabolism-related metabolites.RESULTS Combination of proteomics and Western blot analyses showed increased AMPK activity while the activity of nutrient sensor mTORC1 was decreased by Aramchol in hepatocytes.This translated into changes in the content of their downstream targets including proteins involved in fatty acid(FA)synthesis and oxidation[PACCα/β(S79),SCD1,CPT1A/B,HADHA,and HADHB],oxidative phosphorylation(NDUFA9,NDUFB11,NDUFS1,NDUFV1,ETFDH,and UQCRC2),tricarboxylic acid(TCA)cycle(MDH2,SUCLA2,and SUCLG2),and ribosome(P-p70S6K[T389]and P-S6[S235/S236]).Flux experiments with 13Cuniformely labeled glucose showed that TCA cycle cataplerosis was reduced by Aramchol in hepatocytes,as indicated by the increase in the number of rounds that malate remained in the TCA cycle.Finally,liver metabolomic analysis showed that glucose homeostasis was improved by Aramchol in 0.1MCD fed mice in a dose-dependent manner,showing normalization of glucose,G6P,F6P,UDP-glucose,and Rbl5P/Xyl5P.CONCLUSION Aramchol exerts its effect on glucose and lipid metabolism in NASH through activation of AMPK and inhibition of mTORC1,which in turn activate FAβ-oxidation and oxidative phosphorylation. | David Fernández-Ramos Fernando Lopitz-Otsoa Laura Delacruz-Villar Jon Bilbao Martina Pagano Laura Mosca Maider Bizkarguenaga Marina Serrano-Macia Mikel Azkargorta Marta Iruarrizaga-Lejarreta Jesús Sot Darya Tsvirkun Sebastiaan Martijn van Liempd Felix M Goni Cristina Alonso María Luz Martínez-Chantar Felix Elortza Liat Hayardeny Shelly C Lu JoséM Mato | 2020 | World Journal of Gastroenterology2020，26，34： | 5 |
| 2 | Chronological assessment of mast cell-mediated gut dysfunction and mucosal inflammation in a rat model of chronic psychosocial stress显示文摘 | María Vicario Mar Guilarte Carmen Alonso Pinchang Yang Cristina Martínez Laura Ramos Beatriz Lobo Ana González Meritxell Guilà Marc Pigrau Esteban Saperas Fernando Azpiroz Javier Santos | 2010 | Brain Behavior and Immunity2010，，7： | 1 |
| 3 | Consequence analysis by means of characteristic curves to determine the damage to build- ings from the detonation of explosive substances as a function of TNT equivalence 显示文摘 | Fernando Diaz Alonso Enrique Gonzalez Ferradas Marta Doval Minarro | 2008 | Journal of Loss Prevention in the Process Industries2008，，21： | 1 |
| 4 | BAALC is an important predictor of refractoriness to chemotherapy and poor survival in intermediate-risk acute myeloid leukemia (AML)显示文摘 | Carlos Santamaría María C. Chillón Ramón García-Sanz Cristina Pérez María D. Caballero María V. Mateos Fernando Ramos Alfonso García Coca José M. Alonso Pilar Giraldo Teresa Bernal José A. Queizán Juan N. Rodríguez Noemí Puig Ana Balanzategui María E. Sar | 2010 | Annals of Hematology2010，，5： | 1 |
| 5 | Synthesis and preliminary biological screening of sterol analogues as new antifungal agents against plantpathogens 显示文摘 | Fernando Alonso Adriana M Cirigliano Gabriela M Ca-brera | 2010 | Steroids2010，75，10： | 1 |
| 6 | Extractable soil heavy metals following the cessation of biosolids application to agricultural soil显示文摘 | Walter Ingrid Mart?nez Fernando Alonso Luis | 2002 | Environmental Pollution2002，117，： | 1 |
| 7 | Extractable soil heavy metals following the cessation of biosolids application to agricultural soil显示文摘 | Walter Ingrid Martinez Fernando Alonso Luis | 2002 | Environmental Pollution2002，117，： | 1 |
| 8 | Consequence analysis to determine the damage to humans from vapour cloud explosions using characteristic curves 显示文摘 | Fernando Diaz Alonso Enrique Gonz klez Ferrad's | 2008 | Journal of Hazardous Materials2008，150，1： | 1 |
| 9 | Role of 17β-estradiol administration on insulin sensitivity in the rat: implications for the insulin receptor显示文摘 | Celestino González Ana Alonso Natalia A. Grueso Fernando D??az Manuel M. Esteban Serafina Fernández Angeles M. Patterson | 2002 | Steroids2002，，13： | 1 |
| 10 | Olfaction and neurodegeneration in HD显示文摘 | Fernando A. Barrios Leopoldo Gonzalez Rafael Favila Maria E. Alonso Perla M. Salgado Rosalinda Diaz Juan Fernandez-Ruiz | 2007 | NeuroReport2007，，1： | 1 |
| 11 | The contribution of serum osteoprotegerin to bone mass and vertebral fractures in postmenopausal women显示文摘 | Pedro Mezquita-Raya Magdalena Higuera Diego Fernández García Guillermo Alonso María Estrella Ruiz-Requena Juan Dios Luna Fernando Escobar-Jiménez Manuel Mu?oz-Torres | 2005 | Osteoporosis International2005，，11： | 1 |
| 12 | Residual urinary output in high body mass index individuals on chronic hemodialysis:A disregarded life vest?显示文摘AIM:To assess residual diuresis and diverse variables according to body mass index(BMI).METHODS:Cross-sectional study(n = 57),with 3groups.Group A:BMI < 25,n = 22; Group B:BMI25-30,n = 15; Group C:BMI > 30,n = 20.Diuresis,hematocrit,albumin,C-reactive protein,Malnutrition inflammatory score,Pro-BNP,Troponin T,leptin and insulin levels are expressed as median and ranges(r).RESULTS:Albumin(g/dL):GA vs GC,3.70(r2.20-4.90)vs 3.85(r3.40-4.90),P = 0.02.Diuresis(m L/d):GA690(r0-1780); GB 660(r60-1800); GC 840(r40-2840).Diuresis GA vs GC,P = 0.01.Leptin(ng/m L):GA vs GC,3.81(r0.78-69.60) vs GC,32.80(r0.78-124.50),P< 0.001.Insulin(μU/mL):GA vs GB,7(r2-44) vs 11.50(r4-38),P = 0.02; GA vs GC,7(r2-44) vs 19.5(r5-155),P = 0.0001.Troponin T and Pro-BNP levels were not different.Significant correlations:GC,Insulin-UF:ρ= 0.53; P = 0.03; Troponin T-diuresis:ρ =-0.48,P < 0.05; Pro-BNP-diuresis:ρ =-0.39,P < 0.01; Troponin T-ProB NP:ρ = 0.77,P < 0.0001; albumin-Troponin T:ρ =-0.66,P < 0.0001; albumin-ProB NP:ρ =-0.44,P < 0.05.CONCLUSION:High BMI associated positively with higher diuresis and albuminemia,and negatively with TropT and Pro-BNP.High BMI-associated better survival may be explained by better urinary output,lowering cardiovascular stress. | Hernán Trimarchi María S Ra?a Alejandra Karl José Andrews Mariana Dicugno Vanesa Pomeranz Pablo Young Mariano Forrester Mirta Alonso Fernando Lombi Alexis Muryan | 2014 | World Journal of Nephrology2014，3，4： | 1 |
| 13 | Consequence analysis by meansof characteristic curves to determine the damage tobuildings from the detonation of explosive substances asa function of TNT equivalence显示文摘 | Fernando Diaz Alonso Enrique Gonzalez Ferradas Marta Doval Minarro | 2008 | Journal of LossPrevention in the Process Industries2008，，21： | 1 |
| 14 | Randomized Controlled Trial of Aspiration Needle versus Automated Biopsy Device for Transjugular Liver Biopsy显示文摘 | Rafael Ba?ares Sonia Alonso María-Vega Catalina Marta Casado Diego Rincón Magdalena Salcedo Emilio Alvarez Carmen Guerrero Antonio Echenagusía Fernando Camú?ez Gonzalo Simó | 2001 | Journal of Vascular and Interventional Radiology2001，，5： | 1 |
| 15 | CBF2/DREB1C is a negative regulator of CBF1/DREB1B and CBF3/DREB1A expression and plays a central role in stress tolerance in Arabidopsis显示文摘 | Fernando Novillo José M Alonso Joseph REcker | 2004 | Proc Nati Acad Sci USA2004，101，11： | 1 |
| 16 | Consequence analysis by means of characteristic curves to determinethe damage to buildings from the detonation of explosive substances as a function of TNT equivalence显示文摘 | Fernando Di az Alonso Gonza′lez Enrique Ferrada S | 2008 | Journal of Loss Prevention2008，21，： | 1 |
| 17 | Molecular cloning, characterization and tissue expression of porcine Toll-like receptor 4显示文摘 | Belén álvarez Concepción Revilla Sonia Chamorro Marta López-Fraga Fernando Alonso Javier Domínguez Angel Ezquerra | 2005 | Developmental and Comparative Immunology2005，，4： | 1 |
| 18 | Polymorphisms of antioxidant enzymes, blood pressure and risk of hypertension显示文摘 | María L Mansego Griselda De Marco Solar Mónica Pineda Alonso Fernando Martínez Guillermo T Sáez Juan C Martin Escudero Josep Redón Felipe J Chaves | 2011 | Journal of Hypertension2011，，3： | 1 |
| 19 | Impact of diff erent waves of COVID-19 on emergency medical services and out-of-hospital cardiopulmonary arrest in Madrid, Spain显示文摘Out-of-hospital cardiac arrest(OHCA)is one of the three leading causes of death in industrialized countries.[1,2]Some studies have described the impact of the first COVID-19 pandemic wave in terms of the number of cases and OHCA survival rates in various regions,[3-5]but few have addressed the relationship between the successive phases and how they affected OHCA.[6,7]The 14-d cumulative incidence peaked at more than 990 cases,with these rates remaining above 200 for an eight-month period between 15 March 2020 and 15 March 2021 in Madrid,Spain. | Jose-María Navalpotro-Pascual Diana Monge Martín Manuel-José González León Fernando Neria Serrano Carlos Alonso Blas Belén Muñoz Isabel Yago Muñecas Cuesta Alfredo Carrillo Moya Juan Les González Alonso Mateos Rodríguez | 2022 | World Journal of Emergency Medicine2022，13，5： | 1 |
| 20 | Preliminary stages before nasal reconstruction using forehead flap:restoring perinasal subunits and nostril patency显示文摘Due to the complex three-dimensional structure of the nose,the repair of the nasal defect requires reconstruction of three different layers:skin envelope,osteocartilaginous framework and nasal lining.Before nasal reconstruction can be accomplished,the nose must rest on a stable platform to avoid late nasal obstructions,and septal deviations resulting from scar contraction.We present three cases of nasal reconstruction using a forehead flap in which we performed a preliminary stage to increase reliability of outcomes. | Victor Diniz de Pochat Fernando César Câmara de Oliveira Felipe Simões da Rocha Mata Marcelo Sacramento Cunha Nivaldo Alonso JoséValber Lima Meneses | 2015 | Plastic and Aesthetic Research2015，2，1： | 0 |
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