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| 1 | Hardy spaces H^p over non-homogeneous metric measure spaces and their applications显示文摘Let(X,d,)be a metric measure space satisfying both the geometrically doubling and the upper doubling conditions.Let ρ∈(1,∞),0 | FU Xing LIN Hai Bo YANG Da Chun YANG Dong Yong | 2015 | Science China Mathematics2015，58，2： | 32 |
| 2 | Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis显示文摘骨头转移从疾病是乳癌的经常的复杂并发症和病态和死亡的一个普通原因。在转移期间,分泌蛋白质在在癌症房间和主人基质之间的相互作用起关键作用。为了描绘分泌蛋白质,那与乳癌骨头转移被联系,我们比较四个 MDA-MB-231 (MDA231 ) 衍生物房间的 secretomes 的没有标签的 proteomic 分析与骨头转移的改变的能力衬里的 preformed。128 蛋白质的一个总数被发现是一致地在骨头回归线癌症房间的调节媒介的 up-/down-regulated。改变的蛋白质的充实的分子的函数包括了受体绑定和 peptidase 抑制。通过乳癌房间的另外的 transcriptomic 分析,我们选择了 cystatin E/M (CST6 ) ,在骨头变形的房间下面调整的一个半胱氨酸朊酶禁止者,为进一步功能的研究。我们的结果证明 CST6 压制了乳癌房间的增长,殖民地形成,迁居和侵略。对癌症房间活动性的镇压功能被癌症执行导出房间的可溶的 CST6。更重要地,在癌症房间的 CST6 的宫外的表示在动物学习救了老鼠免于公开 osteolytic 转移和死亡,当时 CST6 击倒的显著地提高的癌症房间骨头转移和弄短的动物幸存。总的来说,我们的学习提供了乳癌骨头向性的全身的 secretome 分析并且作为乳癌 osteolytic 转移的真正的 suppressor 建立了分泌 CST6。 | Lei Jin Yan Zhang Hui Li Ling Yao Da Fu Xuebiao Yao Lisa X Xu Xiaofang Hu Guohong Hu | 2012 | Cell Research2012，22，9： | 15 |
| 3 | Preliminary study on the production of transgenic mice harboring hepatitis B virus X gene显示文摘INTRODUCTIONDespiteoverwhelmingepidemiologicalevidencelinkingpersistentHepatitisBVirus(HBV)infectionandthedevelopmentofhepato... | ZHU Huan Zhang 1, CHENG Guo Xiang 2, CHEN Jian Qu 2, KUANG Shu Yuan 3, CHENG Yong 2, ZHANG Xin Li 1, Ll Hou Da 2, XU Shao Fu 2, SHI Jing Quan 1, QIAN Geng Sun 3 and GU Jian Ren 3 | 1998 | World Journal of Gastroenterology1998，4，6： | 14 |
| 4 | 中西医医学生在叙事医学上的不同视野显示文摘医学作为一种实践科学还是治疗艺术,在现在的医学界有广泛的争论。很多人认为,医学必须要求医生在自身建立叙事情感并且更深入地挖掘病人的内心。文中的调查研究活动以台湾某教学医院的学生为研究对象,开展叙事医学项目的系列活动,采用问卷调查和量表研究,并评估和比较了中西医学生对于叙事医学作为一种学习共情和医学专业精神的方法的不同认知。 | 张千会 周碧雯 Chien - Da Huang Kuo - Chen Liao Fu - Tsai Chuang | 2017 | 中国医学伦理学2017，30，10： | 14 |
| 5 | A Novel Semiconductor CIGS Photovoltaic Material and Thin-Film ED Technology显示文摘In order to achieve low cost high efficiency thin film solar cells,a novel Semiconductor Photovoltaic (PV) active material CuIn 1-x Ga x Se 2 (CIGS) and thin film Electro Deposition (ED) technology is explored.Firstly,the PV materials and technologies is investigated,then the detailed experimental processes of CIGS/Mo/glass structure by using the novel ED technology and the results are reported.These results shows that high quality CIGS polycrystalline thin films can be obtained by the ED method,in which the polycrystalline CIGS is definitely identified by the (112),(204,220) characteristic peaks of the tetragonal structure,the continuous CIGS thin film layers with particle average size of about 2μm of length and around 1 6μm of thickness.The thickness and solar grade quality of CIGS thin films can be produced with good repeatability.Discussion and analysis on the ED technique,CIGS energy band and sodium (Na) impurity properties,were also performed.The alloy CIGS exhibits not only increasing band gap with increasing x ,but also a change in material properties that is relevant to the device operation.The beneficial impurity Na originating from the low cost soda lime glass substrate becomes one prerequisite for high quality CIGS films.These novel material and technology are very useful for low cost high efficiency thin film solar cells and other devices. | ZHENG Guang fu 1,YANG Hong xing 1,MAN Cheuk ho 1,WONG Wing lok 2, AN Da wei 1 and John BURNETT 1(1 Centre for Development of Solar Energy Technology,Department of Building Services Engineering, The Hong Kong Polytechnic University,Hong Kong,C | 2001 | Journal of Semiconductors2001，22，11： | 10 |
| 6 | Advanced data analytics for enhancing building performances: From data-driven to big data-driven approaches显示文摘Buildings have a significant impact on global sustainability.During the past decades,a wide variety of studies have been conducted throughout the building lifecycle for improving the building performance.Data-driven approach has been widely adopted owing to less detailed building information required and high computational efficiency for online applications.Recent advances in information technologies and data science have enabled convenient access,storage,and analysis of massive on-site measurements,bringing about a new big-data-driven research paradigm.This paper presents a critical review of data-driven methods,particularly those methods based on larger datasets,for building energy modeling and their practical applications for improving building performances.This paper is organized based on the four essential phases of big-data-driven modeling,i.e.,data preprocessing,model development,knowledge post-processing,and practical applications throughout the building lifecycle.Typical data analysis and application methods have been summarized and compared at each stage,based upon which in-depth discussions and future research directions have been presented.This review demonstrates that the insights obtained from big building data can be extremely helpful for enriching the existing knowledge repository regarding building energy modeling.Furthermore,considering the ever-increasing development of smart buildings and IoT-driven smart cities,the big data-driven research paradigm will become an essential supplement to existing scientific research methods in the building sector. | Cheng Fan Da Yan Fu Xiao Ao Li Jingjing An Xuyuan Kang | 2021 | Building Simulation2021，14，1： | 10 |
| 7 | Mechanism of exogenous nucleic acids and their precursors improving the repair of intestinal epithelium after 7-irradiation in mice显示文摘AIM To clone expressed genes associated withrepair of irradiation-damaged mice intestinalgland cells treated by small intestinal RNA,andto explore the molecular mechanism ofexogenous nucleic acids improving repair ofintestinal crypt.METHODS The animal mode of test group andcontrol group was established,forty-five micebeing irradiated by γ ray were treated with smallintestinal RNA as test group,forty mice beingirradiated by γ ray were treated withphysiological saline as control group,five micewithout irradiation were used as normal control,their jejunal specimens were collectedrespectively at 6h,12h,24h,4d and 8d afterirradiation.Then by using LD-PCR based onsubtractive hybridization,these gene fragmentsdifferentially expressed between test group andcontrol group were obtained,and then werecloned into T vectors as well as beingsequenced.Obtained sequences were screenedagainst.GeneBank,if being new sequences,they were submitted to GeneBank.RESULTS Ninety clones were associated withrepair of irradiation-damaged intestinal glandcells treated by intestinal RNA.These clonesfrom test group of 6h,12h,24h,4d and 8dwere respectively 18,22,25,13,12.By screening against GeneBank,18 of which werenew sequences,the others were dramaticallysimilar to the known sequences,mainly similarto hsp,Nmi,Dutt1,alkaline phosphatase,homeobox,anti-CEA ScFv antibody,arginine/serine kinase and BMP-4,repA.Eighteen genefragments were new sequences,their acceptnumbers in GeneBank were respectivelyAF240164-AF240181.CONCLUSION Ninety clones were obtained tobe associated with repair of irradiation-damagedmice intestinal gland cells treated by smallintestinal RNA,which may be related toabnormal expression of genes and matchedproteins of hsp,Nmi,Duttl,Na,K-ATPase,alkalineph-osphatase,glkA,single strandedreplicative centromeric gene as well as 18 newsequences. | Da Xiang Cui~1 Guei Ying Zeng~2 Feng Wang~1 Jun Rong Xu~1 Dong Qing Ren~2 Yan Hai Guo~1 Fu Rong Tian~2 Xiao Jun Yan~1 Yu Hou~1 Cheng Zhi Su~1 1 Institute of Genetic Diagnosis of the Fourth Military Medical University,Xi’an 710032,China 2 Department of Irradiation Medicine of the Fourth Military Medical University,Xi’an 710032,China | 2000 | World Journal of Gastroenterology2000，6，5： | 8 |
| 8 | Prognostic factors and therapeutic effects of different treatment modalities for colorectal cancer liver metastases显示文摘BACKGROUND Colorectal cancer(CRC)is one of the most common malignant tumors in China,and the liver is the most common metastatic site in patients with advanced CRC.Hepatectomy is the gold standard treatment for colorectal liver metastases.For patients who cannot undergo radical resection of liver metastases for various reasons,ablation therapy,interventional therapy,and systemic chemotherapy can be used to improve their quality of life and prolong their survival time.AIM To explore the prognostic factors and treatments of liver metastases of CRC.METHODS A retrospective analysis was conducted on 87 patients with liver metastases from CRC treated at the Liaoning Cancer Hospital and Institute between January 2005 and March 2011.According to different treatments,the patients were divided into the following four groups:Surgical resection group(36 patients);ablation group(23 patients);intervention group(15 patients);and drug group(13 patients).The clinicopathological data and postoperative survival of the four groups were analyzed.The Kaplan-Meier method was used for survival analysis,and the Cox proportional hazards regression model was used for multivariate analysis.RESULTS The median survival time of the 87 patients was 38.747±3.062 mo,and the 1-and 3-year survival rates were 87.5%and 53.1%,respectively.The Cox proportional hazards model showed that the following factors were independent factors affecting prognosis:The degree of tumor differentiation,the number of metastases,the size of metastases,and whether the metastases are close to great vessels.The results of treatment factor analysis showed that the effect of surgical treatment was better than that of drugs,intervention,or ablation alone,and the median survival time was 48.83±4.36 mo.The drug group had the worst prognosis,with a median survival time of only 13.5±0.7 mo(P<0.05).For patients with liver metastases of CRC near the great vessels,the median survival time(27.3 mo)of patients undergoing surgical resection was better than that of patients using other treatments(20.6 mo)(P<0.05).CONCLUSION Patients with a low degree of primary tumor differentiation,multiple liver metastases(number of tumors>4),and maximum diameter of liver metastases>5 cm have a poor prognosis.Among drug therapy,intervention,ablation,and surgical treatment options,surgical treatment is the first choice for liver metastases.When liver metastases are close to great vessels,surgical treatment is significantly better than drug therapy,intervention,and ablation alone. | Zuo-Hong Ma Yong-Peng Wang Wen-Heng Zheng Ji Ma Xue Bai Yong Zhang Yuan-He Wang Da Chi Xi-Bo Fu Xiang-Dong Hua | 2020 | World Journal of Gastrointestinal Oncology2020，12，10： | 7 |
| 9 | The promoter analysis of the human C17orf25 gene, a novel chromosome 17pl3.3 gene显示文摘The human C17orf25 gene (Accession No. AF177342) is one of thirteen genes cloned from a region displaying a high score of loss of heterozygosity within chromosome 17p13.3 in human hepatocellular car-cinoma in China[l]. To unveil the underlying mechanisms for the transcription regulation of this gene and understand its implication to the hepatocellular carcinogenesis, we looked into the relevant aspects by both bioinformatic and experimental executions. We found: 1, The abundant expression of the C17orf25 gene was evident in all the cell lines and tissue samples tested, showing little hepatoma-selectivity; 2, Its tran-scription starts at a single site, locating at -60 from the translation initiation codon; 3, A 58 bp fragment containing the transcription start, extending from -112 to -55, represents the minimal promoter; 4, The consensus sequence within this fragment recognized by SP1 contributes predominantly to the activity of the minimal promoter; 5, The bioinformatic analysis suggests that the C17ort25 gene may encode a protein in the family of the glyoxalase. Our data has provided some deep insight into both function and regulation of the C17orf25 gene in the context of the normal liver and hepatocellular carcinoma. | JIAN YING GUO, JIAN XU, DA QIN MAO, LI LI FU, JIAN REN GU, JING DE ZHUThe State-Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Ln 2200/25, Xie-Tu Road, Shanghai 200032, China | 2002 | Cell Research2002，12，5： | 7 |
| 10 | Advances in lithium niobate photonics:development status and perspectives显示文摘Lithium niobate(LN)has experienced significant developments during past decades due to its versatile properties,especially its large electro-optic(EO)coefficient.For example,bulk LN-based modulators with high speeds and a superior linearity are widely used in typical fiber-optic communication systems.However,with everincreasing demands for signal transmission capacity,the high power and large size of bulk LN-based devices pose great challenges,especially when one of its counterparts,integrated silicon photonics,has experienced dramatic developments in recent decades.Not long ago,high-quality thin-film LN on insulator(LNOI)became commercially available,which has paved the way for integrated LN photonics and opened a hot research area of LN photonics devices.LNOI allows a large refractive index contrast,thus light can be confined within a more compact structure.Together with other properties of LN,such as nonlinear/acousto-optic/pyroelectric effects,various kinds of high-performance integrated LN devices can be demonstrated.A comprehensive summary of advances in LN photonics is provided.As LN photonics has experienced several decades of development,our review includes some of the typical bulk LN devices as well as recently developed thin film LN devices.In this way,readers may be inspired by a complete picture of the evolution of this technology.We first introduce the basic material properties of LN and several key processing technologies for fabricating photonics devices.After that,various kinds of functional devices based on different effects are summarized.Finally,we give a short summary and perspective of LN photonics.We hope this review can give readers more insight into recent advances in LN photonics and contribute to the further development of LN related research. | Guanyu Chen Nanxi Li Jun Da Ng Hong-Lin Lin Yanyan Zhou Yuan Hsing Fu Lennon Yao Ting Lee Yu Yu Ai-Qun Liu Aaron J.Danner | 2022 | Advanced Photonics2022，4，3： | 6 |
| 11 | Review:Recent advances in multisensor multitarge11racking using random finite set显示文摘In this study,we provide an overview of recent advances in multisensor multitarget tracking based on the random finite set(RFS)approach.The fusion that plays a fundamental role in multisensor filtering is classified into data-level multitarget measurement fusion and estimate-level multitarget density fusion,which share and fuse local measurements and posterior densities between sensors,respectively.Important properties of each fusion rule including the optimality and sub-optimality are presented.In particulax,two robust multitarget density-averaging approaches,arithmetic-and geometric-average fusion,are addressed in detail for various RFSs.Relevant research topics and remaining challenges are highlighted. | Kai DA Tiancheng LI Yongfeng ZHU Hongqi FAN Qiang FU | 2021 | Frontiers of Information Technology & Electronic Engineering2021，22，1： | 5 |
| 12 | Efficacy and Economic Evaluation of Nonbiological Artificial Liver Therapy in Acute-on-chronic Hepatitis B Liver Failure显示文摘Background and Aims:Nonbiological artificial liver(NBAL)is frequently used as a first-line treatment for hepatitis B virus-associated acute-on-chronic liver failure(HBV-ACLF).This study aimed to compare the therapeutic efficacy and cost-effectiveness ratio(CER)of comprehensive medical treatment,plasma exchange(PE),and double plasma molecular adsorption system(DPMAS)plus half-dose PE(DPMAS+PE)in patients with HBV-ACLF.Methods:A total of 186 patients with HBV-ACLF randomly received comprehensive medical treatment,PE,or DPMAS+PE and were prospectively evaluated.Patients were divided into four subgroups based on the pretreatment prothrombin activity(PTA):Group I(PTA>40%),group II(PTA 30–40%),group III(PTA 20–30%),and group IV(PTA<20%).The main outcome measures were 28 day effectiveness;90 day liver transplantation-free survival;change of biochemical parameters;and CER.Results:DPMAS+PE treatment was associated with significantly higher 28 day effectiveness and 90 day liver transplantation-free survival compared with PE treatment in patients with group I liver failure.Clearance of serum total bilirubin(TBIL),AST,and creatinine(Cr)were significantly higher in the DPMAS+PE group than in the PE group.For subjects with group I liver failure,DPMAS+PE treatment had advantages of lower CER values and better cost-effectiveness.Conclusions:Compared with comprehensive medical treatment and PE alone,DPMAS with halfdose sequential PE treatment more effectively improved TBIL,AST,and Cr in HBV-ACLF patients,improved 28 day effectiveness and 90 day survival rates in patients with group I liver failure,and was more cost effective.DPMAS+PE is a viable NBAL approach for treatment of HBV-ACLF. | Cichun Wu Wenting Peng Da Cheng Huimin Gu Fei Liu Shifang Peng Lei Fu | 2023 | Journal of Clinical and Translational Hepatology2023，11，2： | 5 |
| 13 | A Remark on the Boundedness of Calder6n-Zygmund Operators in Non-homogeneous Spaces显示文摘让μ是可能非加倍的 R^d 上的一项氡措施。μ满足的唯一的状况是那μ(B (x, r )) 为所有 x ∈ R^d 的≤ Cr^n, r > 0 并且一些修理了 0 < n ≤ d。在这篇论文,作者证明从 H^1 的围住的海角(μ) 进 L^(1,∞)( 有 Hoermander 类型的 Calderon-Zygmund 核的单个不可分的操作员 T 的μ) 暗示 itsL^2 (μ)-boundedness。 | Xiao Li FU Guo En HU Da Chun YANG | 2007 | Acta Mathematica Sinica,English Series2007，23，3： | 4 |
| 14 | Molten salt synthesis, formation mechanism, and oxidation behavior of nanocrystalline HfB2 powders显示文摘Nanocrystalline Hf B2 powders were successfully synthesized by molten salt synthesis technique at 1373 K using B and Hf O2 as precursors within KCl/Na Cl molten salts.The results showed that the as-synthesized powders exhibited an irregular polyhedral morphology with the average particle size of 155 nm and possessed a single-crystalline structure.From a fundamental aspect,we demonstrated the molten-salt assisted formation mechanism that the molten salts could accelerate the diffusion rate of the reactants and improve the chemical reaction rate of the reactants in the system to induce the synthesis of the high-purity nanocrystalline powders.Thermogravimetric analysis showed that the oxidation of the as-synthesized Hf B2 powders at 773–1073 K in air was the weight gain process and the corresponding oxidation behavior followed parabolic kinetics governed by the diffusion of oxygen in the oxide layer. | Da LIU Qiangang FU Yanhui CHU | 2020 | Journal of Advanced Ceramics2020，9，1： | 4 |
| 15 | The First Outbreak of Omicron Subvariant BA.5.2—Beijing Municipality,China,July 4,2022显示文摘On July 4,2022,the first case of Omicron subvariant BA.5.2 in Beijing Municipality was discovered in Yanqing District.The case was a 49-yearold Chinese male who had arrived in Shanghai Municipality via international flight DL9927 from North Carolina,U.S.on June 15.He stayed in a hotel for the 14-day arrival quarantine and was discharged on June 30.He arrived in Beijing via domestic flight MU5103 on July 1 and was transferred point-to-point from the airport to his residence community in Yanqing District.On July 3,his sample was collected through community mass screening and reported positive in the next morning.The case had received 3 doses of Moderna’s mRNA vaccines in the U.S.,with the last shot on May 26,2022. | Zhaomin Feng Ying Shen Shuang Li Jia Li Shaohua Wang Zhenquan Zhang Yunkui Shen Fu Li Yang Pan Quanyi Wang Da Huo | 2022 | China CDC weekly2022，4，30： | 4 |
| 16 | A New Triterpene from Cephalomappa sinensis显示文摘A new triterpene 1, 1(, 2(, 3(-trihydroxylup-20 (29) -ene was isolated from Cepha-lomappa sinensis. The structure was elucidated mainly on the basis of 1D and 2D NMR spectral means. | Wen Li MEI, Yun Bao MA, Shao Hua WU, Hao Fu DAI, Da Gang WU* Laboratory of Phytochemistry, Kunming Institute of Botany, Academia Sinica, Kunming 650204 | 2001 | Chinese Chemical Letters2001，12，6： | 4 |
| 17 | The Survey of Cronobacter spp.(formerly Enterbacter sakazakii) in Infant and Follow-up Powdered Formula in China in 2012显示文摘Objective To determine Cronobacter spp.contamination in infant and follow-up powdered formula in China.Methods All of 2282 samples were collected from the retail markets in China from January 2012 to December 2012,and analyzed for Cronobacter spp.by the Chinese National Food Safety Standard.Characterization of the isolates was analyzed by pulsed-field gel electrophoresis(PFGE)with XbaI and Spe I restriction enzymes.Results Cronobacter spp.strains were isolated from 25 samples,and the positive rates in infant powdered formulas and follow-up powdered formulas were 0.90%(10/1011)and 1.18%(15/1271),respectively.Analysis of variable data regarding different purchasing store formats,seasonality,and production locations as well as comparison of infant versus follow-up formulas did not reveal statistically significant factors.During the sampling period,one of six surveillance zones did exhibit a statistically significant trend towards higher positive rate.PFGE characterization of Cronobacter spp.to elucidate genetic diversity revealed only three pairs of Cronobacter spp.out of 25 having the same PFGE patterns.Conclusion The current investigation indicated a lower positive rate of Cronobacter spp.in the powdered formula in China.This evidence suggested contamination originating from multiple different sources during the manufacturing process. | PEI Xiao Yan YAN Lin ZHU Jiang Hui LI Ning GUO Yun Chang FU Ping JIA Hua Yun ZHANG Xiu Li YANG Xiao Rong YANG Da Jin | 2016 | Biomedical and Environmental Sciences2016，29，2： | 4 |
| 18 | 经自然腔道取标本手术(NOSES)治疗结直肠癌的国际共识显示文摘近年来,经自然腔道取标本手术(NOSES)治疗结直肠癌已引起了广泛关注。NOSES应用于结直肠手术的潜在优势包括减轻术后疼痛,减少伤口并发症,减少对术后镇痛的需求,加快肠道功能的恢复,缩短住院时间,更好的美容效果,以及给患者带来了更佳的心理状态。尽管NOSES的手术创伤显著减轻,然而该技术也存在一些潜在问题。尤其随着这项新技术的兴起,人们对其无菌的把控、肿瘤学结果和病例选择等问题表示出关切。因此,迫切需要一部操作指南来规范NOSES手术在结直肠癌中的开展。经过国际NOSES联盟全体成员的三轮讨论,最终形成了该部共识。这一共识有助于NOSES在全球范围内的长远发展。 | Xu Guan Zheng Liu Antonio Longo Jian-Chun Cai William Tzu-Liang Chen Lu-Chuan Chen Ho-Kyung Chun Joaquim Manuel da Costa Pereira Sergey Efetov Ricardo Escalante Qing-Si He Jun-Hong Hu Cuneyt Kayaalp Seon-Hahn Kim Jim S.Khan Li-Jen Kuo Atsushi Nishimura Fernanda Nogueira Junji Okuda Avanish Saklani Ali A.Shafik Ming-Yin Shen Jung-Tack Son Jun-Min Song Dong-Hui Sun Keisuke Uehara Gui-Yu Wang Ye Wei Zhi-Guo Xiong Hong-Liang Yao Gang Yu Shao-Jun Yu Hai-Tao Zhou Suk-Hwan Lee Petr V.Tsarkov Chuan-Gang Fu Xi-Shan Wang The International Alliance of NOSES | 2019 | Gastroenterology Report2019，7，1： | 3 |
| 19 | Effect of long-term fertilization on soil nitrate distribution显示文摘A thirteen years long term field fertilizer experiment was conducted to monitor the effect of different fertilization on soil nitrate distribution. The results showed: (1) Applying relative excessive N fertilizer could result large quantities of NO - 3 residue and NO - 3 movement downward in soil profiles; amending phosphate fertilizer or organic manure with nitrogen fertilizer together could significantly improve the status of NO - 3 leaching downward due to the balanced uptake of nutrients by crops. (2) Appropriate amounts of nitrogen fertilizer which was equal or smaller than the optimal fertilization rate could not result in more NO - 3 leaching in Northern China. (3) Precipitation influenced the amounts and depth of soil NO\+-\-3 leaching: NO - 3 could move to 80 cm depth or below at autumn or at the next spring when rainfall was higher during the rainy season through July to September in North China. | GUO Li ping *, ZHANG Fu suo, WANG Xing ren, MAO Da ru, CHEN Xin ping (Department of Plant Nutrition, China Agricultural University, Beijing 100094, China. E mail: guolp@ns.ami.ac.cn) | 2001 | Journal of Environmental Sciences2001，13，1： | 3 |
| 20 | Insulin-like growth factor 1-induced enolase 2 deacetylation by HDAC3 promotes metastasis of pancreatic cancer显示文摘Enolase 2(ENO2)is a key glycolytic enzyme in the metabolic process of glycolysis,but its potential function in pancreatic ductal adenocarcinoma(PDAC)is unclear.In this study,we observed a significant overexpression of ENO2 in PDAC tissues,and its expression was correlated with metastasis and poor prognosis in PDAC patients.K394 was identified as a major acetylation site in ENO2 that regulates its enzymatic activity,cell metabolism and PDAC progression.Knockdown of ENO2 suppressed tumor growth and liver metastasis in PDAC.Re-expression of wild-type(WT)ENO2,but not the K394 acetylation mimetic mutant,could reverse the decreased tumor malignancy.We further characterized histone deacetylase 3(HDAC3)and P300/CBP-associated factor(PCAF)as the potential deacetylase and acetyltransferase for ENO2,respectively.HDAC3-mediated deacetylation was shown to lead to ENO2 activation and enhancement of glycolysis.Importantly,insulin-like growth factor-1(IGF-1)was found to decrease K394 acetylation and stimulate ENO2 activity in a dose-and time-dependent manner.The PI3K/AKT/mTOR pathway facilitated the phosphorylation of HDAC3 on S424,which promoted K394 deacetylation and activation of ENO2.Linsitinib,an oral small-molecule inhibitor of IGF-1R,could inhibit IGF-1-induced ENO2 deacetylation by HDAC3 and the PI3K/AKT/mTOR pathway.Furthermore,linsitinib showed a different effect on the growth and metastasis of PDAC depending on the overexpression of WT versus K394-mutant ENO2.Our results reveal a novel mechanism by which acetylation negatively regulates ENO2 activity in the metastasis of PDAC by modulating glycolysis.Blockade of IGF-1-induced ENO2 deacetylation represents a promising strategy to prevent the development of PDAC. | Yan Zheng Chao Wu Jimeng Yang Yue Zhao Huliang Jia Min Xue Da Xu Feng Yang Deliang Fu Chaoqun Wang Beiyuan Hu Ze Zhang Tianen Li Shican Yan Xuan Wang Peter J.Nelson Christiane Bruns Lunxiu Qin Qiongzhu Dong | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 3 |
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