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| 1 | Significance and relationship between Yes-associated protein and survivin expression in gastric carcinoma and precancerous lesions显示文摘AIM:To analyze the differences and relevance of Yes-associated protein (YAP) and survivin, and to explore the correlation and signifi cance of their expression in gastric carcinoma and precancerous lesions.METHODS: The PV9000 immunohistochemical method was used to detect the expression of YAP and survivin in 98 cases of normal gastric mucosa, 58 intestinal metaplasia (IM), 32 dysplasia and 98 gastric carcinoma.RESULTS: The positive rates of YAP in dysplasia (37.5%) and gastric carcinoma (48.0%) were significantly higher than that in normal gastric mucosa (13.3%), P<0.01. The positive rates of survivin in IM (53.4%), dysplasia (59.4%) and gastric carcinoma (65.3%) were significantly higher than in normal gastric mucosa (11.2%), P<0.01. Survivin expression gradually increased from 41.7% in well differentiated adenocarcinoma through 58.3% in moderately differentiated adenocarcinoma to 75.6% in poorly differentiated adenocarcinoma, with significant Rank correlation, rk=0.279, P<0.01. The positive rate of survivin in gastric carcinoma of diffused type (74.6%) was significantly higher than that in intestinal type (51.3%), P<0.05. In gastric carcinoma with lymph node metastasis (76.9%), the positive rate of survivin was signifi cantly higher than that in the group without lymph node metastasis (41.2%), P<0.01. In 98 cases of gastric carcinoma, the expression of YAP and of survivin were positively correlated, rk=0.246, P<0.01.CONCLUSION: YAP may play an important role as a carcinogenic factor and may induce survivin expression. Detecting both markers together may help in early diagnosis of gastric carcinoma. | Chun-Li Da Yan Xin Jing Zhao Xiang-Dong Luo | 2009 | World Journal of Gastroenterology2009，15，32： | 53 |
| 2 | A community-derived classification for extant lycophytes and ferns显示文摘发展史长通知了蕨类植物分类。当我们推断进化的树的能力改善了,针对认出生来的组的分类变得逐渐地预兆、稳定。这里,我们为 lycophytes 和蕨纲植物提供一个现代、全面分类,在下面,利用一条基于社区的途径类水平。我们 monophyly 用作主要标准让 taxa,而且目的识别保存两个广泛地被接受的存在 taxa 和界限并且与我们蕨类植物发展史的理解一致。总共,这个分类对待一在 337 个类, 51 个家庭, 14 目,和二个班估计了 11 916 种类。这个分类没在 lycophyte 和蕨纲植物上作为最后的词被打算分类,而是当前的假设的概括陈述,源于最好的可得到的数据并且在问题由熟悉植物的那些大多数塑造了。我们希望它将在蕨类植物上为最近的文学的那些想要的参考用作一个资源发展史和分类,为指导未来调查的一个框架,和推进讲话的刺激。 | Eric Schuettpelz Harald Schneider Alan R. Smith Peter Hovenkamp Jefferson Prado Germinal Rouhan Alexandre Salino Michael Sundue Thafs Elias Almeida Barbara Parris Emily B. Sessa Ashley R. Field Andre Luis de Gasper Carl J. Rothfels Michael D. Windham Marcus Lehnert Benjamin Dauphin Atsushi Ebihara Samuli Lehtonen Pedro Bond Schwartsburd Jordan Metzgar Li-Bing Zhang Li-Yaung Kuo Patrick J. Brownsey Masahiro Kato Marcelo Daniel Arana Francine C. Assis Michael S. Barker David S. Barrington Ho-Ming Chang Yi-Han Chang Yi-Shan Chao Cheng-Wei Chen De-Kui Chen Wen-Liang Chiou Vinicius Antonio de Oliveira Dittrich Yi-Fan Duan Jean-Yves Dubuisson Donald R. Farrar Susan Fawcett Jose Maria Gabriel y Galan Luiz Armando de Araujo Goes-Neto Jason R. Grant Amanda L. Grusz Christopher Haufler Warren Hauk Hai He Sabine Hennequin Regina Yoshie Hirai Layne Huiet Michael Kessler Petra Korall Paulo H. Labiak Anders Larsson Blanca Leen Chun-Xiang Li Fay-Wei Li Melanie Link-Perez Hong-Mei Liu Ngan Thi Lu Esteban I. Meza-Torres Xin-Yuan Miao Robbin Moran Claudine Massi Mynssens Nathalie Nagalingum Benjamin Ollgaard Alison M. Paul Jovani B. de S. Pereira Leon R. Perrie Monica Ponce Tom A. Ranker Christian Schulz Wataru Shinohara Alexander Shmakov Erin M. Sigel Filipe Soares de Souza Lana da Silva Sylvestre Weston Testo Luz Amparo Triana-Moreno Chie Tsutsumi Hanna Tuomisto IvAn A. Valdespino Alejandra Vasco Raquel Stauffer Viveros Alan Weakley Ran Wei Stina Weststrand Paul G. Wolf George Yatskievych Xiao-Gang Xu Yue-Hong Yan Liang Zhang Xian-Chun Zhang Xin-Mao Zhou | 2016 | Journal of Systematics and Evolution2016，54，6： | 44 |
| 3 | Role and limitation of FMPSPGR dynamic contrast scanning in the follow-up of patients with hepatocellular carcinoma treated by TACE显示文摘AIM:To evaluate the role and limitation of fast multiplanarspoiled gradient-recalled (FMPSPGR) MR dynamiccontrast scanning in the follow-up of patients with HCCtreated by transarterial chemoembolization (TACE).METHODS:Twenty-two patients with 24 HCC lesionsconfirmed by biopsy or surgical resection underwentMR imaging in 4-9wks after TACE with a superconducting1.5 T MR scanner, including SE T1WI, T2WI and FMPSPGRdynamic contrast scanning. The signal intensities of alllesions on SE T1WI, T2WI and the enhancement patternson FMPSPGR dynamic contrast scanning were observed,and the comparison was made between MRI findingsand pathological results in ail the cases.RESULTS:Of the 24 lesions, the signal intensities werevarious on SE T1WI and T2WI. On T1WI, 13 lesionsappeared as hyperintense, 4 lesions were isointenseand the other 7 lesions were hypointensese.Histologically, hyperintense lesions showed on T1WIwere viable tumor or hemorrhage; isointensities werecoagulative necrosis or inflammatory infiltration;hypointensities were tumor, liquified necrosis,coagulative necrosis or inflammatory infiltration. OnT2WI, 15 lesions appeared as hyperintense, 3 lesionswere isointense and the other 6 lesions werehypointensese. Hyperintense lesions showed on T2WIwere residuals of viable tumor, hemorrhage, liquefiednecrosis or inflammatory infiltration; isointense lesionswere residuals of viable tumor or inflammatoryinfiltration; hypointense lesions were coagulativenecrosis. On FMPSPGR dynamic contrast scanning, 18of the 24 lesions enhanced on early-phase dynamicscanning conesponding to residuals of viable tumor andthe other 6 lesions had no enhancement at this phasebecause complete necrosis were seen in the histologicexamination. On delayed-phase dynamic scanning, 6lesions had permanent enhancement appeared asinhomogeneous hyperintensity and both residuals ofviable tumor and inflammatory infiltration were foundby histologic examination. 18 lesions were hypointenseat this phase and 8 of them coexisted with peripheralring-like enhancement of the lesions resulting fromviable tumors or inflammatory infiltration.CONCLUSION: FMPSPGR MR dynamic contrast scanningcan reflect the pathologic changes of HCC treated byTACE. Especially, early-phase dynamic scanning canevaluate accurately residuals of viable tumor andnecrosis in HCC lesions. FMPSPGR dynamic contrastscanning is useful in the follow-up of patients with HCCtreated by TACE combined with SE T1WI and T2WI, butit is difficult to differentiate peripheral viable tumorsfrom inflammatory infiltration. | Fu-Hua Yan Kang-Rong Zhou Jie-Min Cheng Jian-Hua Wang Zhi-Ping Yan Reng-Rong Da Department of Radiology,Zhongshan Hospital,Fudan University,Shanghai 200032,China Jia Fan Department of Hepatobiliary-surgery,Zhongshan Hospital,Fudan University,Shanghai 200032,China Yuan Ji Department of Pathology,Zhongshan Hospital,Fudan University,Shanghai 200032,China | 2002 | World Journal of Gastroenterology2002，8，4： | 32 |
| 4 | The DTH8-Hd1 Module Mediates Day-Length-Dependent Regulation of Rice Flowering显示文摘Photoperiodic flowering 是最重要的小径之一在米饭(Oryza sativa ) 管理 flowering,在哪个标题日期 1 (Hd1 ) , Arabidopsis CONSTANS 基因的 ortholog,编码一个枢轴的管理者。Hd1 在短天的条件(SD ) 下面支持 flowering,但是镇压在由调整领导日期 3a (Hd3a ) 的表示的长天的条件(LD ) 下面的 flowering,在米饭的 FLOWERING 地点 T (英尺) ortholog。然而, Hd1 怎么响应天长度改变它的规章的活动的分子的机制仍然保持大部分未知。在这研究,我们证明在由 Hd1 的 LD 的 flowering 的压抑依赖于到领导 8 的抄写因素天(DTH8 ) 。DTH8 功能的损失由 Hd1 导致 Hd3a 的激活,导致早 flowering。我们发现 Hd1 直接与 DTH8 交往并且 DTH8-Hd1 建筑群的形成为由在 LD 的 Hd1 的 Hd3a 的 transcriptional 压抑是必要的,含有 Hd1 功能的开关被 DTH8 在 LD 而非在 SD 调停。而且,我们表明 DTH8 与 Hd3a 倡导者联系在 Hd3a 地点调制 H3K27 trimethylation (H3K27me3 ) 的水平。面对 DTH8-Hd1 建筑群, H3K27me3 水平在 Hd3a 被增加,而 DTH8 功能的损失在 Hd3a 导致了减少的 H3K27me3 水平。一起拿,我们的调查结果显示响应天长度, DTH8 在调停起一个关键作用 ? 由通过到在 photoperiodic flowering 的形状 epigenetic 修正的 DTH8-Hd1 模块的 Hd1 的 Hd3a 的 transcriptional 规定。 | Anping Dul Wei Tian Menghao Wei Wei Yan Hang He Da Zhou Xi Huang Shigui Li Xinhao Ouyang | 2017 | Molecular Plant2017，10，7： | 21 |
| 5 | Effectiveness of Different Waist Circumference Cut-off Values in Predicting Metabolic Syndrome Prevalence and Risk Factors in Adults in China显示文摘Objective To study the effectiveness of waist circumference cut-off values in predicting the prevalence of metabolic syndrome(MetS) and risk factors in adults in China. Methods A cross-sectional survey was condcuted in 14 provinces(autonomous region, municipality) in China. A total of 47 325 adults aged ≥20 years were selected by multistage stratified sampling, and questionnaire survey and physical and clinical examination were conducted among them. MetS was defined according to the International Diabetes Federation(IDF) criteria and modified IDF criteria.Results The age-standardized prevalence of MetS was 24.2%(22.1% in men and 25.8% in women) and 19.5%(22.1% in men and 18.0% in women) according to the IDF criteria and modified IDF criteria respectively. The age-standardized prevalence of pre-MetS was 8.1%(8.6% in men and 7.8% in women) according to the modified IDF criteria. The prevalence of MetS was higher in urban residents than rural residents and in northern China residents than in southern China residents. The prevalence of central obesity was about 30% in both men and women according to the ethnicity-specific cut-off values of waist circumference for central obesity(90 cm for men and 85 cm for women). Multivariate regression analysis revealed no significant difference in risk factors between the two MetS definitions. Conclusion Using both the modified IDF criteria and ethnicity-specific cut-off values of waist circumference can provide more useful information about the prevalence of MetS in China. | ZHOU Hai Cheng LAI Ya Xin SHAN Zhong Yan JIA Wei Ping YANG Wen Ying LU Ju Ming WENG Jian Ping JI Li Nong LIU Jie TIAN Hao Ming JI Qiu He ZHU Da Long CHEN Li GUO Xiao Hui ZHAO Zhi Gang Li Qiang ZHOU Zhi Guang GE Jia Pu SHAN Guang Liang | 2014 | Biomedical and Environmental Sciences2014，27，5： | 17 |
| 6 | Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis显示文摘骨头转移从疾病是乳癌的经常的复杂并发症和病态和死亡的一个普通原因。在转移期间,分泌蛋白质在在癌症房间和主人基质之间的相互作用起关键作用。为了描绘分泌蛋白质,那与乳癌骨头转移被联系,我们比较四个 MDA-MB-231 (MDA231 ) 衍生物房间的 secretomes 的没有标签的 proteomic 分析与骨头转移的改变的能力衬里的 preformed。128 蛋白质的一个总数被发现是一致地在骨头回归线癌症房间的调节媒介的 up-/down-regulated。改变的蛋白质的充实的分子的函数包括了受体绑定和 peptidase 抑制。通过乳癌房间的另外的 transcriptomic 分析,我们选择了 cystatin E/M (CST6 ) ,在骨头变形的房间下面调整的一个半胱氨酸朊酶禁止者,为进一步功能的研究。我们的结果证明 CST6 压制了乳癌房间的增长,殖民地形成,迁居和侵略。对癌症房间活动性的镇压功能被癌症执行导出房间的可溶的 CST6。更重要地,在癌症房间的 CST6 的宫外的表示在动物学习救了老鼠免于公开 osteolytic 转移和死亡,当时 CST6 击倒的显著地提高的癌症房间骨头转移和弄短的动物幸存。总的来说,我们的学习提供了乳癌骨头向性的全身的 secretome 分析并且作为乳癌 osteolytic 转移的真正的 suppressor 建立了分泌 CST6。 | Lei Jin Yan Zhang Hui Li Ling Yao Da Fu Xuebiao Yao Lisa X Xu Xiaofang Hu Guohong Hu | 2012 | Cell Research2012，22，9： | 15 |
| 7 | On-treatment monitoring of liver fibrosis with serum hepatitis B core-related antigen in chronic hepatitis B显示文摘BACKGROUND Non-invasive evaluation for liver fibrosis is clinically important,especially in patients with undetectable hepatitis B virus(HBV)DNA treated with nucleoside analogs.AIM To clarify the monitoring power of hepatitis B core-related antigen(HBcrAg)for hepatic histologic changes in patients with chronic hepatitis B(CHB)treated with entecavir.METHODS This prospective multicenter study used multiple ordinal and multivariate logistics regression analysis to assess variables associated with Ishak fibrosis score and regression for fibrosis regression,respectively,in 403 CHB patients,including 374 with entecavir for 72 weeks(291 underwent paired liver biopsy)and 29 as controls.RESULTS Level of HBcrAg correlated negatively with liver fibrosis staging(γ=-0.357,P<0.001)in hepatitis B e antigen(HBeAg)-positive patients,and positively with liver fibrosis staging in HBeAg-negative patients.Higher HBcrAg concentration was associated with younger age,HBeAg positive status,high HBV DNA loads,high level of hepatitis B surface antigen(HBsAg)and higher necroinflammation,but not with HBV genotype.Serum concentration of HBcrAg,basal core promoter/precore(BCP/PC)mutant,quantitation of HBsAg(qHBsAg)and platelet counts were independently associated with Ishak fibrosis score on multiple ordinal regression.HBV DNA was undetectable in 88.37%of patients treated with entecavir at week 72,while their level of HBcrAg was still detectable.A greater reduction in post-treatment HBcrAg concentration was associated with the regression of hepatic fibrosis and histological improvement.HBcrAg concentration>6.33 log IU/mL at baseline and logarithmic reduction>1.03 log IU/mL at week 72 were associated with a higher chance of regression of liver fibrosis and histological improvement,respectively.CONCLUSION HBcrAg level is associated with liver fibrosis progression.HBcrAg is an excellent monitor of hepatic histological changes,especially in CHB patients treated with nucleoside analogs. | Xiu-Juan Chang Chao Sun Yan Chen Xiao-Dong Li Zu-Jiang Yu Zheng Dong Wen-Lin Bai Xiao-Dong Wang Zhi-Qin Li Da Chen Wen-Juan Du Hao Liao Qi-Yu Jiang Li-Jun Sun Yin-Yin Li Cui-Hong Zhang Dong-Ping Xu Yong-Ping Chen Qin Li Yong-Ping Yang | 2019 | World Journal of Gastroenterology2019，25，32： | 12 |
| 8 | In situ optical backpropagation training of diffractive optical neural networks显示文摘Training an artificial neural network with backpropagation algorithms to perform advanced machine learning tasks requires an extensive computational process.This paper proposes to implement the backpropagation algorithm optically for in situ training of both linear and nonlinear diffractive optical neural networlks,which enables the acceleration of training speed and improvement in energy efficiency on core computing modules.We demonstrate that the gradient of a loss function with respect to the weights of diffractive layers can be accurately calculated by measuring the forward and backward propagated optical fields based on light reciprocity and phase conjunction principles.The diffractive modulation weights are updated by programming a high-speed spatial light modulator to minimize the error between prediction and target output and perform inference tasks at the speed of light.We numerically validate the effectiveness of our approach on simulated networks for various applications.The proposed in situ optical learning architecture achieves accuracy comparable to in silico training with an electronic computer on the tasks of object dlassification and matrix-vector multiplication,which further allows the diffractive optical neural network to adapt to system imperfections.Also,the self-adaptive property of our approach facilitates the novel application of the network for all-optical imaging through scattering media.The proposed approach paves the way for robust implementation of large-scale difractive neural networks to perform distinctive tasks all-optically. | TIANKUANG ZHOU LU FANG TAO YAN JIAMIN WU YIPENG LI JINGTAO FAN HUAQIANG WU XING LIN QIONGHAI DA | 2020 | Photonics Research2020，8，6： | 11 |
| 9 | Key nodes of a micro RNA network associated with the integrated mesenchymal subtype of high-grade serous ovarian cancer显示文摘Metastasis is the main cause of cancer mortality. One of the initiating events of cancer metastasis of epithelial tumors is epithelial-to-mesenchymal transition(EMT), during which cells dedifferentiate from a relatively rigid cell structure/morphology to a flexible and changeable structure/morphology often associated with mesenchymal cells. The presence of EMT in human epithelial tumors is reflected by the increased expression of genes and levels of proteins that are preferentially present in mesenchymal cells. The combined presence of these genes forms the basis of mesenchymal gene signatures, which are the foundation for classifying a mesenchymal subtype of tumors. Indeed, tumor classification schemes that use clustering analysis of large genomic characterizations, like The Cancer Genome Atlas(TCGA), have defined mesenchymal subtype in a number of cancer types, such as high-grade serous ovarian cancer and glioblastoma. However, recent analyses have shown that gene expression-based classifications of mesenchymal subtypes often do not associate with poor survival. This 'paradox' can be ameliorated using integrated analysis that combines multiple data types. We recently found that integrating m RNA and micro RNA(mi RNA) data revealed an integrated mesenchymal subtype that is consistently associated with poor survival in multiple cohorts of patients with serous ovarian cancer. This network consists of 8 major mi RNAs and 214 m RNAs. Among the 8 mi RNAs, 4 are known to be regulators of EMT. This review provides a summary of these 8 mi RNAs, which were associated with the integrated mesenchymal subtype of serous ovarian cancer. | Yan Sun Fei Guo Marina Bagnoli Feng-Xia Xue Bao-Cun Sun Ilya Shmulevich Delia Mezzanzanica Ke-Xin Chen Anil K. Sood Da Yang Wei Zhang | 2015 | Chinese Journal of Cancer2015，34，1： | 10 |
| 10 | Human organoids in basic research and clinical applications显示文摘Organoids are three-dimensional(3D)miniature structures cultured in vitro produced from either human pluripotent stem cells(hPSCs)or adult stem cells(AdSCs)derived from healthy individuals or patients that recapitulate the cellular heterogeneity,structure,and functions of human organs.The advent of human 3D organoid systems is now possible to allow remarkably detailed observation of stem cell morphogens,maintenance and differentiation resemble primary tissues,enhancing the potential to study both human physiology and developmental stage.As they are similar to their original organs and carry human genetic information,organoids derived from patient hold great promise for biomedical research and preclinical drug testing and is currently used for personalized,regenerative medicine,gene repair and transplantation therapy.In recent decades,researchers have succeeded in generating various types of organoids mimicking in vivo organs.Herein,we provide an update on current in vitro differentiation technologies of brain,retinal,kidney,liver,lung,gastrointestinal,cardiac,vascularized and multi-lineage organoids,discuss the differences between PSC-and AdSC-derived organoids,summarize the potential applications of stem cell-derived organoids systems in the laboratory and clinic,and outline the current challenges for the application of organoids,which would deepen the understanding of mechanisms of human development and enhance further utility of organoids in basic research and clinical studies. | Xiao-Yan Tang Shanshan Wu Da Wang Chu Chu Yuan Hong Mengdan Tao Hao Hu Min Xu Xing Guo Yan Liu | 2022 | Signal Transduction and Targeted Therapy2022，7，6： | 10 |
| 11 | Advanced data analytics for enhancing building performances: From data-driven to big data-driven approaches显示文摘Buildings have a significant impact on global sustainability.During the past decades,a wide variety of studies have been conducted throughout the building lifecycle for improving the building performance.Data-driven approach has been widely adopted owing to less detailed building information required and high computational efficiency for online applications.Recent advances in information technologies and data science have enabled convenient access,storage,and analysis of massive on-site measurements,bringing about a new big-data-driven research paradigm.This paper presents a critical review of data-driven methods,particularly those methods based on larger datasets,for building energy modeling and their practical applications for improving building performances.This paper is organized based on the four essential phases of big-data-driven modeling,i.e.,data preprocessing,model development,knowledge post-processing,and practical applications throughout the building lifecycle.Typical data analysis and application methods have been summarized and compared at each stage,based upon which in-depth discussions and future research directions have been presented.This review demonstrates that the insights obtained from big building data can be extremely helpful for enriching the existing knowledge repository regarding building energy modeling.Furthermore,considering the ever-increasing development of smart buildings and IoT-driven smart cities,the big data-driven research paradigm will become an essential supplement to existing scientific research methods in the building sector. | Cheng Fan Da Yan Fu Xiao Ao Li Jingjing An Xuyuan Kang | 2021 | Building Simulation2021，14，1： | 10 |
| 12 | Mycoplasma pneumoniae Macrolide Resistance and MLVA Typing in Children in Beijing,China,in 2016:Is It Relevant?显示文摘Objective The aim of this study is to investigate the macrolide resistance rate and molecular type with multiple-locus variable-number tandem-repeat analysis(MLVA)of Mycoplasma pneumoniae of Beijing in 2016 in pediatric patients.Methods Real-time quantitative polymerase chain reaction(PCR)was used to identify M.pneumoniae,and MLVA was performed.The domain V of the 23 S rRNA was sequenced to detect macrolide-resistant point mutations.We also investigated the activities of antibiotics against M.pneumoniae isolates in vitro.Results The PCR detection rate of M.pneumoniae in children in Beijing was 40%,and the macrolide resistance rate was 66%.The A2063 G mutation in the 23 S rRNA V region is the dominant mutation(137/146,93.84%),whereas the A2064 G mutation is rare(9/146,6.16%).Seventy-three samples were typed successfully by MLVA typing,including 86.3%(63/73)were MLVA type 4-5-7-2,and 13.7%(10/73)were MLVA type 3-5-6-2.No other types were found.No strains were resistant to levofloxacin or tetracycline.Conclusion In 2016,a specific decrease in the macrolide resistance rate occurred in Beijing.The detection rate and macrolide resistance rate of outpatients are lower than those of inpatients.The A2063 G mutants M.pneumoniae have high levels of resistance to erythromycin and azithromycin.The primary MLVA type is 4-5-7-2,followed by 3-5-6-2.No other MLVA types were detected.No strains resistant to tetracycline or levofloxacin were found in vitro. | DOU Hai Wei TIAN Xiu Jun XIN De Li WEI Ran ZHOU Wei WANG Hong QIN Xuan Guang SHAO Jun Yan XU Bao Ping GE Li Xia SHI Da Wei | 2020 | Biomedical and Environmental Sciences2020，33，12： | 9 |
| 13 | A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts显示文摘Terminally differentiated cells can be generated by lineage reprogramming,which is,however,hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming. | Bingqing Xie Da Sun Yuanyuan Du Jun Jia Shicheng Sun Jun Xu Yifang Liu Chengang Xiang Sitong Chen Huangfan Xie Qiming Wang Guangya Li Xuehui LYU Hui Shen Shiyu Li Min Wu Xiaonan Zhang Yue Pu Kuanhui Xiang Weifeng Lai Peng Du Zhenghong Yuan Cheng Li Yan Shi Shichun Lu Hongkui Deng | 2019 | Cell Research2019，29，9： | 9 |
| 14 | Clinicopathological significance of B-cell-specific Moloney murine leukemia virus insertion site 1 expression in gastric carcinoma and its precancerous lesion显示文摘AIM: To explore the relation between B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi-1) expression and the clinicopathological features of gastric carcinoma (GC). METHODS: Immunohistochemistry was used to detect the expression of Bmi-1 and ki-67. Double-labeling staining was used to display the distribution of Bcl-2+/ki-67- cells in 162 cases of GC and its matched normal mucosa and precancerous lesion. RESULTS: The positive rate of Bmi-1 expression in GC (52.5%) was significantly higher than that in normal gastric mucosa (21.6%, χ2 = 33.088, P < 0.05). The Bmi-1 expression in GC was closely related with the Lauren's and Borrmann's classification and clinical stage (χ2 = 4.400, 6.122 and 11.190, respectively, P < 0.05). The expression of ki-67 was related to the Borrmann's classification (χ2 = 13.380, P < 0.05). Bcl-2 expression was correlated with the Lauren's classification (χ2 = 4.725, P < 0.05), and the Bmi-1expression both in GC (rk = 0.157, P < 0.05) and in intestinal metaplasia (rk = 0.270, P < 0.05). CONCLUSION: Abnormal Bmi-1 expression in GC may be involved in cell proliferation, apoptosis and cancerization. This marker can objectively indicate the clinicopathological characteristics of GC. | Jing Zhao Xiang-Dong Luo Chun-Li Da Yan Xin | 2009 | World Journal of Gastroenterology2009，15，17： | 9 |
| 15 | Cloned pigs derived from somatic cell nuclear transferembryos cultured in vitro atlow oxygen tension显示文摘Pig cloning has great potential to human xenotransplantation. The present study was designed to establish a more efficient system for producing cloned pigs by somatic cell nuclear transfer (SCNT). Our approach was as follows: SCNT embryos were reconstructed by using fetal fibroblasts of Chinese miniature pig as donors and in vitro matured oocytes of prepubertal gilts as recipients. Reconstructed em- bryos were induced by electrical fusion/activation and cultured in BSA-containing North Carolina State University 23 medium (NCSU-23) or Porcine Zygote Medium (PZM-3) at the gas condition of 5% CO2, 7% O2, 88% N2. A total of 230 cloned embryos were transferred to three surrogate sows, producing three piglets. One of them is apparently healthy. The clonal provenance of the piglet was indicated by its coat color and confirmed by DNA microsatellite analysis. These results indicate that the use of in vitro matured oocytes from prepubertal gilts as recipient, combined with cloned embryos cultured at low oxygen tension is an effective way to produce cloned pigs. | PAN Dengke ZHANG Yunhai SUN Xiuzhu ZHANG Jian LI Xuyang LI Yan GU Zhiliang DA Yunping WU Changxin LI Ning | 2006 | Chinese Science Bulletin2006，51，7： | 8 |
| 16 | Boundedness of Some Maximal Commutators in Hardy-type Spaces with Non-doubling Measures显示文摘让μ是满足一些仅仅生长条件的 R^d 上的一项非否定的氡措施。在这个假设下面,在一些强壮类型的空格的围住的海角为是 Calderon-Zygmund 操作符和 RBMO 产生的平常的最大的整流器的变体的最大的 Calderon-Zygmund 操作符和最大的整流器的一个类被建立(μ)函数,在强壮类型的空格是一些的地方,拨出潜水艇空格,与考虑 RBMO 联系了(μ)函数,强壮的空格 H^1 ( Tolsa 的μ)。 | Guo En HU Yan MENG Da Chun YANG | 2007 | Acta Mathematica Sinica,English Series2007，23，6： | 8 |
| 17 | Can ultrasound predict the severity of acute pancreatitis early by observing acute fluid collection?显示文摘INTRODUCTIONThe spectrum of acute pancreatitis (AP) ranges from a mild spontaneously resolved disorder to severe disease with mortality up to 20%-48.4%[1-3]. sAP is defined as the AP with organ failure and /or local complications which developed form acute fluid collection (AFC) including necrosis ,abscess , pseudocyst formation into or around the pancreas [4]. | Yan Luo1 Chao Xin Yuan1 Yu Lan Peng1 Pei Lin Wei1 Zhao Da Zhang2 Jun Ming Jiang3 Lin Dai1 Yun Kai Hu1 1Ultrasound Department of First Affiliated Hospital of West China University of Medical Science, Chengdu 610041, Sichuan Province, China2Surgery Department of First Affiliated Hospital of West China University of Medical Science, Chengdu 610041, Sichuan Province, China3Tranditional Chinese Medicine, Department of First Affiliated Hospital of West China University of Medical Science, Chengdu 610041, Sichuan Province, China | 2001 | World Journal of Gastroenterology2001，7，2： | 8 |
| 18 | Mechanism of exogenous nucleic acids and their precursors improving the repair of intestinal epithelium after 7-irradiation in mice显示文摘AIM To clone expressed genes associated withrepair of irradiation-damaged mice intestinalgland cells treated by small intestinal RNA,andto explore the molecular mechanism ofexogenous nucleic acids improving repair ofintestinal crypt.METHODS The animal mode of test group andcontrol group was established,forty-five micebeing irradiated by γ ray were treated with smallintestinal RNA as test group,forty mice beingirradiated by γ ray were treated withphysiological saline as control group,five micewithout irradiation were used as normal control,their jejunal specimens were collectedrespectively at 6h,12h,24h,4d and 8d afterirradiation.Then by using LD-PCR based onsubtractive hybridization,these gene fragmentsdifferentially expressed between test group andcontrol group were obtained,and then werecloned into T vectors as well as beingsequenced.Obtained sequences were screenedagainst.GeneBank,if being new sequences,they were submitted to GeneBank.RESULTS Ninety clones were associated withrepair of irradiation-damaged intestinal glandcells treated by intestinal RNA.These clonesfrom test group of 6h,12h,24h,4d and 8dwere respectively 18,22,25,13,12.By screening against GeneBank,18 of which werenew sequences,the others were dramaticallysimilar to the known sequences,mainly similarto hsp,Nmi,Dutt1,alkaline phosphatase,homeobox,anti-CEA ScFv antibody,arginine/serine kinase and BMP-4,repA.Eighteen genefragments were new sequences,their acceptnumbers in GeneBank were respectivelyAF240164-AF240181.CONCLUSION Ninety clones were obtained tobe associated with repair of irradiation-damagedmice intestinal gland cells treated by smallintestinal RNA,which may be related toabnormal expression of genes and matchedproteins of hsp,Nmi,Duttl,Na,K-ATPase,alkalineph-osphatase,glkA,single strandedreplicative centromeric gene as well as 18 newsequences. | Da Xiang Cui~1 Guei Ying Zeng~2 Feng Wang~1 Jun Rong Xu~1 Dong Qing Ren~2 Yan Hai Guo~1 Fu Rong Tian~2 Xiao Jun Yan~1 Yu Hou~1 Cheng Zhi Su~1 1 Institute of Genetic Diagnosis of the Fourth Military Medical University,Xi’an 710032,China 2 Department of Irradiation Medicine of the Fourth Military Medical University,Xi’an 710032,China | 2000 | World Journal of Gastroenterology2000，6，5： | 8 |
| 19 | Two new phenylethanoid glycosides and a new secoiridoid glycoside from the roots of Picrorhiza scrophulariiflora显示文摘二新 phenylethanoid glycosides,命名 scroside H (1 ) , scroside 我(2 ) ,并且新 secoiridoid glycoside,命名 picrogentioside 我(3 ) ,从地下被孤立 Picrorhiza scrophulariiflora 的部分。他们的结构根据分光镜的证据被阐明。 | Lian Chun Zou Zhi Hui Yan Tong Fei Zhu Hua Xiang Da Cheng Wang Xu Ming Deng | 2010 | Chinese Chemical Letters2010，21，9： | 7 |
| 20 | An action-based Markov chain modeling approach for predicting the window operating behavior in office spaces显示文摘Reliable energy and performance prediction for building design and planning is important for newly-designed or retrofitted buildings.Window operating behavior has an important influence on the ventilation and energy consumption of these buildings under different realistic scenarios.Therefore,quantitatively describing this behavior and constructing a prediction model are important.In this work,an action-based Markov chain modeling approach for predicting window operating behavior in office spaces was proposed.Two summer measurement data(2016 and 2018)were used to verify the accuracy and validity of the modeling approach.The opening rate,outdoor temperature,time distribution,and on-off curve were proposed as four inspection standards.This study also compared the prediction performance between the action-based Markov chain modeling approach with the state-based Markov chain modeling approach,which is the most popular modeling approach to model occupant window operating behavior.This study proved that the yearly variation of occupants’behavior performed a form of action that remained unchanged during a certain period.Meanwhile,the results also proved that the action-based Markov chain modeling approach can reflect the actual window operating behavior accurately within an open-plan office,which is a beneficial supplement for energy-consumption simulation software in a window-state prediction module.The state-based Markov chain modeling approach showed better stability and accuracy in terms of the opening rate,whereas the action-based Markov chain modeling approach showed good consistency with the measurement data in the on-off curves and in situations with little data.For the on-off curves,the accuracy of action-based modeling approach in the prediction of window open-state is 20%higher. | Xin Zhou Tiance Liu Da Yan Xing Shi Xing Jin | 2021 | Building Simulation2021，14，2： | 7 |
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